International journal of systematic and evolutionary microbiology, Jan 2, 2015
Eight strains representing two novel anamorphic yeast species consisted of six strains isolated f... more Eight strains representing two novel anamorphic yeast species consisted of six strains isolated from the external surface of rice leaves (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124 and YE-156) and a corn leaf (DMKU-CP430T) collected in Thailand, and two strains isolated from a composite flower (11-1114) and a fallen dead leaf (12-301) collected in Belize. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were assigned to be two novel Hannaella species. The seven strains (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124, YE-156, 11-1114 and 12-301) differed from each other by 0-3 nucleotide substitutions in the D1/D2 region and 0-1 nucleotide substitution in the ITS region. In terms of pairwise sequences similarity of the D1/D2 region these seven strains were closest to Hannaella zeae but with 1.2-1.7% (7-9) nucleotide substitutions. The sequence of ITS region of these seven strains differed fro...
International journal of systematic and evolutionary microbiology, Jan 2, 2015
Two yeast strains representing two novel yeast species were isolated from frass of an unidentifie... more Two yeast strains representing two novel yeast species were isolated from frass of an unidentified insect (ST-78) and the external surfaces of rice leaves (YE170) collected in Thailand. The two strains are genetically, morphologically and phenotypically distinct from recognized species and were found to represent two novel Yamadazyma species although formation of ascospores was not observed. In terms of pairwise sequence similarity of the D1/D2 region of the large subunit (LSU) rRNA gene, the closest species of the strain ST-78 was Candida lessepsii but with 3.8% nucleotide substitutions, while the closest species of the strain YE170 was the strain ST-78 but with 4.3% nucleotide substitutions. Analysis of the sequences of the internal transcribed spacer 1 and 2 (ITS1-2) regions revealed that strain ST-78 differed from C. lessepsii by 8.8% nucleotide substitutions and from strain YE170 by 9.4% nucleotide substitutions. The result of pairwise sequence similarity of the D1/D2 and ITS1-...
The Journal of General and Applied Microbiology, 2004
Six strains of anamorphic yeasts isolated from insect frass collected in several regions of Thail... more Six strains of anamorphic yeasts isolated from insect frass collected in several regions of Thailand were assigned to the genus Candida based on the conventional taxonomic criteria used for yeast classification. These strains have Q-7 as the major ubiquinone and are suggested to have close relationships to the genus Pichia. Three strains, ST-225, ST-228 and ST-229, have identical nucleotide sequences in the D1/D2 domain of 26S rDNA and are closely related to Pichia japonica, but differ by six nucleotides (1.1% ) from this species. These three strains are considered to represent a single new species, which is described as Candida easanensis sp. nov. Two strains, ST-311 and ST-320, have identical sequences in the D1/D2 domain and resemble Pichia veronae and Pichia fabianii but differ from them by nine nucleotides (1.6%) in D1/D2 sequences. The two strains are described as Candida pattaniensis sp. nov. The remaining strain, ST-37, is related to Pichia americana and Pichia bimundalis but differs by six(1.1%) and seven (1.2%) nucleotides from these species, respectively. This strain is described as Candida nakhonratchasimensis sp. nov.
The Journal of General and Applied Microbiology, 2007
Two strains of anamorphic yeasts isolated from insect frass collected in southern Thailand were a... more Two strains of anamorphic yeasts isolated from insect frass collected in southern Thailand were assigned to the genus Candida based on the conventional taxonomic criteria used for yeast classification. In the phylogenetic tree based on the D1/D2 domain of the 26S rDNA, these strains are distant from the known species of yeasts and considered to represent two different new species. They are named Candida kazuoi sp. nov. and Candida hasegawae sp. nov.
The Journal of General and Applied Microbiology, 2010
Fifteen strains of anamorphic yeasts isolated from various natural substrates collected in variou... more Fifteen strains of anamorphic yeasts isolated from various natural substrates collected in various places in Thailand were found to represent two novel species of anamorphic yeast genus Candida based on the sequence analysis of the D1/D2 domain of the large subunit rRNA genes, chemotaxonomic and conventional properties used for the classification of yeasts. These strains are located in the clade including Candida etchellsii and Candida magnoliae. Fourteen strains represented by ST-490(T) (BCC 15176(T)=NBRC 106439(T)= CBS 11674(T)) are closely related to Candida sorbosivorans in the D1/D2 sequences but 11 nucleotides (2.4%) were substituted. The remaining strain, ST-594(T) (=BCC 15278(T)=NBRC 106446(T)=CBS 11673(T)) showed a close relationship to Candida geochares but 21 nucleotides (4.7%) were substituted. Apparently, these strains represent two novel Candida species of the Starmerella clade. The two species are described as Candida potacharoeniae sp. nov. and Candida spenceri sp. nov. in the present paper. Like the most species of this clade, the two species contain galactose in the cells in addition to glucose and mannose and have high mol% G + C of 54.4-55.9 and 54.9, respectively.
The Journal of General and Applied Microbiology, 2011
Four strains of yeasts isolated in Thailand and Taiwan were found to represent four distinct nove... more Four strains of yeasts isolated in Thailand and Taiwan were found to represent four distinct novel species of the ascomycetous anamorphic yeast genus Candida. These strains are located in the Clavispora-Metschnikowia clade in a phylogenetic tree based on the D1/D2 domain sequences of the large subunit rRNA genes. Together with Candida picinguabensis and Candida saopaulonensis, the four novel species constitute a well-separated subclade from other species of the Clavispora-Metschnikowia clade. Three species from Thailand are described as Candida bambusicola sp. nov. (type strain, ST-50(T) = BCC 7750(T) = NBRC 106734(T) = CBS 11723(T)), Candida nongkhaiensis sp. nov. (type strain, ST-95(T) = BCC 8331(T) = NBRC 105874(T) =CBS 11724(T)) and Candida succicola sp. nov. (type strain, ST-631(T) = BCC 15314(T) = NBRC 106736(T) = CBS 11726(T)), respectively, and the species from Taiwan is described as Candida touchengensis sp. nov. (type strain, SY4S03(T) = NBRC 102647(T) = BCRC 23097(T) = CBS 10585(T)).
A yeast strain isolated from insect frass collected in Thailand was found to represent a new spec... more A yeast strain isolated from insect frass collected in Thailand was found to represent a new species of the genus Pichia. It is described as Pichia nongkratonensis sp. nov. In the phylogenetic tree based on the D1/D2 domain sequences of 26S rDNA, this yeast constitutes a cluster with Pichia dryadoides with high bootstrap confidence level; however, it differs from the
ABSTRACT A strain of yeast isolated from insect frass collected in Thailand was found to represen... more ABSTRACT A strain of yeast isolated from insect frass collected in Thailand was found to represent a hitherto undescribed species of a basidiomycetous anamorphic genus Trichosporon. It is described as Trichosporon siamense. In the phylogenetic tree based on the D1/D2 region sequences of 26S rDNA, this yeast constitutes a cluster with several Q-9 having species of Trichosporon including T. otae and T. brassicae but is clearly differentiated from these species by 1.8% or more base substitutions. In the internal transcribed spacer region (ITS1 and ITS2), this species differs from T. scarabaeorum, the nearest species, by 6.5% base substitution.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2012
Two strains (NT29(T) and NT31(T)) of xylose-assimilating yeasts were obtained from soils collecte... more Two strains (NT29(T) and NT31(T)) of xylose-assimilating yeasts were obtained from soils collected in northern Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer region, the two strains were found to represent two novel ascomycete yeast species. Strain NT29(T) was assigned to the genus Candida belonging to the Pichia clade as a representative of Candida phayaonensis sp. nov.; the type strain is NT29(T) (=BCC 47634(T)=NBRC 108868(T)=CBS 12319(T)). Strain NT31(T) represented a novel Wickerhamomyces species, which was named Wickerhamomyces xylosica sp. nov.; the type strain is NT31(T) (=BCC 47635(T)=NBRC 108869(T)=CBS 12320(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2011
Five strains (RV5(T), RV140, R31(T), RS17 and RS28(T)) representing three novel anamorphic ascomy... more Five strains (RV5(T), RV140, R31(T), RS17 and RS28(T)) representing three novel anamorphic ascomycetous yeast species were isolated by membrane filtration from estuarine waters collected from a mangrove forest in Laem Son National Park, Ranong Province, Thailand, on different occasions. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and the internal transcribed spacer region and phylogenetic analysis, three strains were found to represent two novel Candida species. Two strains (RV5(T) and RV140) represented a single novel species, for which the name Candida laemsonensis sp. nov. is proposed. The type strain is RV5(T) (=BCC 35154(T) =NBRC 105873(T) =CBS 11419(T)). Strain R31(T) was assigned to a novel species that was named Candida andamanensis sp. nov. (type strain R31(T) =BCC 25965(T) =NBRC 103862(T) =CBS 10859(T)). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and phylogenetic analysis, strains RS17 and RS28(T) represented another novel species of Candida, for which the name Candida ranongensis sp. nov. is proposed. The type strain is RS28(T) (=BCC 25964(T) =NBRC 103861(T) =CBS 10861(T)).
Six yeast strains of a novel anamorphic yeast species were isolated from natural samples collecte... more Six yeast strains of a novel anamorphic yeast species were isolated from natural samples collected in Thailand (RV60 T and LYSM9), Taiwan (SC5L04 and GE19S05), and Japan (JCM 11058 and JCM 11059). Analysis of the D1/D2 domain of the large subunit rRNA gene sequences revealed that the sequences of five strains (LYSM9, SC5L04, GE19S05, JCM 11058, and JCM 11059) were identical and differed from the sequence of strain RV60 T by only one nucleotide substitution. The closest recognized species in terms of pairwise sequence similarity was Candida abiesophila, but the level of nucleotide substitution (14.2-14.3%) was sufficient to justify the description of a separate species. Phenotypic characteristics of the six strains were almost the same except for two strains from Japan, which showed the ability to ferment glucose and grew in the presence of 10% NaCl and 5% glucose while the others did not. The phenotypic characteristics of the six strains included proliferation by multilateral budding, absence of ascospores, arthrospores, and ballistospores, and negative Diazonium blue B and urease tests. The major ubiquinone was Q-7. On the basis of the above findings, the six strains were assigned to a single novel species of Candida, for which the name Candida asiatica sp. nov. is proposed. The type strain is RV60 T (BCC 25966 T = NBRC 103863 T = CBS 10863 T ).
Three yeast stains were isolated from two unknown fruits (strains DD2-22-1 T and SK44) and moss (... more Three yeast stains were isolated from two unknown fruits (strains DD2-22-1 T and SK44) and moss (strain in Thailand. Analysis of the D1/ D2 domain of the large subunit (LSU) rRNA gene sequences of the three strains revealed that they belonged to the same species. In terms of pairwise sequence similarity, Candida cf. glabrata UWO(PS) 98-110.4 and Candida nivariensis were the closest undescribed and recognized taxa, but the levels of nucleotide substitutions were 1.7-1.9% and 2.0-2.2%, respectively. The levels of nucleotide substitutions were sufficient to justify the description of a separate species of Candida. In the phylogenetic tree based on the D1/D2 domain of the LSU rRNA gene the three strains were placed in a separate branch in the Nakaseomyces clade with C. cf. glabrata UWO(PS)98-110.4, C. nivariensis, Candida glabrata, Candida bracarensis, Candida kungkrabaensis and Nakaseomyces delphensis. Phenotypic characteristics of the three strains were similar which included proliferation by multilateral budding, absence of ascospores, arthrospores or ballistospores; negative for Diazonium blue B and urease tests. The major ubiquinone was Q-6. On the basis of the above findings, the three strains were assigned to a single novel species of Candida, for which the name Candida uthaithanina sp. nov is proposed. The type strain is DD2-22-1 T (= BCC 29899 T = NBRC 104876 T = CBS 10932 T ).
Teleomorphic species of the genus Zygozyma and anamorphic species of the genus Myxozyma were exam... more Teleomorphic species of the genus Zygozyma and anamorphic species of the genus Myxozyma were examined phylogenetically. In phylogenetic trees based on 18S rRNA-, 26S rRNA-, mitochondrial small subunit rRNA-and EF-1α-gene sequences and concatenated sequences of the latter four regions, derived from the neighbor-joining method, the four species of the genus Zygozyma constituted four clusters, respectively, with low bootstrap values, indicating that all four species can be distinguished from one another at the generic level. The name of Limtongia was newly suggested for Zygozyma smithiae, and Limtongia smithiae was proposed as new combination.
International journal of systematic and evolutionary microbiology, Jan 2, 2015
Eight strains representing two novel anamorphic yeast species consisted of six strains isolated f... more Eight strains representing two novel anamorphic yeast species consisted of six strains isolated from the external surface of rice leaves (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124 and YE-156) and a corn leaf (DMKU-CP430T) collected in Thailand, and two strains isolated from a composite flower (11-1114) and a fallen dead leaf (12-301) collected in Belize. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were assigned to be two novel Hannaella species. The seven strains (DMKU-RP72T, DMKU-RP109, DMKU-RP119, YE-124, YE-156, 11-1114 and 12-301) differed from each other by 0-3 nucleotide substitutions in the D1/D2 region and 0-1 nucleotide substitution in the ITS region. In terms of pairwise sequences similarity of the D1/D2 region these seven strains were closest to Hannaella zeae but with 1.2-1.7% (7-9) nucleotide substitutions. The sequence of ITS region of these seven strains differed fro...
International journal of systematic and evolutionary microbiology, Jan 2, 2015
Two yeast strains representing two novel yeast species were isolated from frass of an unidentifie... more Two yeast strains representing two novel yeast species were isolated from frass of an unidentified insect (ST-78) and the external surfaces of rice leaves (YE170) collected in Thailand. The two strains are genetically, morphologically and phenotypically distinct from recognized species and were found to represent two novel Yamadazyma species although formation of ascospores was not observed. In terms of pairwise sequence similarity of the D1/D2 region of the large subunit (LSU) rRNA gene, the closest species of the strain ST-78 was Candida lessepsii but with 3.8% nucleotide substitutions, while the closest species of the strain YE170 was the strain ST-78 but with 4.3% nucleotide substitutions. Analysis of the sequences of the internal transcribed spacer 1 and 2 (ITS1-2) regions revealed that strain ST-78 differed from C. lessepsii by 8.8% nucleotide substitutions and from strain YE170 by 9.4% nucleotide substitutions. The result of pairwise sequence similarity of the D1/D2 and ITS1-...
The Journal of General and Applied Microbiology, 2004
Six strains of anamorphic yeasts isolated from insect frass collected in several regions of Thail... more Six strains of anamorphic yeasts isolated from insect frass collected in several regions of Thailand were assigned to the genus Candida based on the conventional taxonomic criteria used for yeast classification. These strains have Q-7 as the major ubiquinone and are suggested to have close relationships to the genus Pichia. Three strains, ST-225, ST-228 and ST-229, have identical nucleotide sequences in the D1/D2 domain of 26S rDNA and are closely related to Pichia japonica, but differ by six nucleotides (1.1% ) from this species. These three strains are considered to represent a single new species, which is described as Candida easanensis sp. nov. Two strains, ST-311 and ST-320, have identical sequences in the D1/D2 domain and resemble Pichia veronae and Pichia fabianii but differ from them by nine nucleotides (1.6%) in D1/D2 sequences. The two strains are described as Candida pattaniensis sp. nov. The remaining strain, ST-37, is related to Pichia americana and Pichia bimundalis but differs by six(1.1%) and seven (1.2%) nucleotides from these species, respectively. This strain is described as Candida nakhonratchasimensis sp. nov.
The Journal of General and Applied Microbiology, 2007
Two strains of anamorphic yeasts isolated from insect frass collected in southern Thailand were a... more Two strains of anamorphic yeasts isolated from insect frass collected in southern Thailand were assigned to the genus Candida based on the conventional taxonomic criteria used for yeast classification. In the phylogenetic tree based on the D1/D2 domain of the 26S rDNA, these strains are distant from the known species of yeasts and considered to represent two different new species. They are named Candida kazuoi sp. nov. and Candida hasegawae sp. nov.
The Journal of General and Applied Microbiology, 2010
Fifteen strains of anamorphic yeasts isolated from various natural substrates collected in variou... more Fifteen strains of anamorphic yeasts isolated from various natural substrates collected in various places in Thailand were found to represent two novel species of anamorphic yeast genus Candida based on the sequence analysis of the D1/D2 domain of the large subunit rRNA genes, chemotaxonomic and conventional properties used for the classification of yeasts. These strains are located in the clade including Candida etchellsii and Candida magnoliae. Fourteen strains represented by ST-490(T) (BCC 15176(T)=NBRC 106439(T)= CBS 11674(T)) are closely related to Candida sorbosivorans in the D1/D2 sequences but 11 nucleotides (2.4%) were substituted. The remaining strain, ST-594(T) (=BCC 15278(T)=NBRC 106446(T)=CBS 11673(T)) showed a close relationship to Candida geochares but 21 nucleotides (4.7%) were substituted. Apparently, these strains represent two novel Candida species of the Starmerella clade. The two species are described as Candida potacharoeniae sp. nov. and Candida spenceri sp. nov. in the present paper. Like the most species of this clade, the two species contain galactose in the cells in addition to glucose and mannose and have high mol% G + C of 54.4-55.9 and 54.9, respectively.
The Journal of General and Applied Microbiology, 2011
Four strains of yeasts isolated in Thailand and Taiwan were found to represent four distinct nove... more Four strains of yeasts isolated in Thailand and Taiwan were found to represent four distinct novel species of the ascomycetous anamorphic yeast genus Candida. These strains are located in the Clavispora-Metschnikowia clade in a phylogenetic tree based on the D1/D2 domain sequences of the large subunit rRNA genes. Together with Candida picinguabensis and Candida saopaulonensis, the four novel species constitute a well-separated subclade from other species of the Clavispora-Metschnikowia clade. Three species from Thailand are described as Candida bambusicola sp. nov. (type strain, ST-50(T) = BCC 7750(T) = NBRC 106734(T) = CBS 11723(T)), Candida nongkhaiensis sp. nov. (type strain, ST-95(T) = BCC 8331(T) = NBRC 105874(T) =CBS 11724(T)) and Candida succicola sp. nov. (type strain, ST-631(T) = BCC 15314(T) = NBRC 106736(T) = CBS 11726(T)), respectively, and the species from Taiwan is described as Candida touchengensis sp. nov. (type strain, SY4S03(T) = NBRC 102647(T) = BCRC 23097(T) = CBS 10585(T)).
A yeast strain isolated from insect frass collected in Thailand was found to represent a new spec... more A yeast strain isolated from insect frass collected in Thailand was found to represent a new species of the genus Pichia. It is described as Pichia nongkratonensis sp. nov. In the phylogenetic tree based on the D1/D2 domain sequences of 26S rDNA, this yeast constitutes a cluster with Pichia dryadoides with high bootstrap confidence level; however, it differs from the
ABSTRACT A strain of yeast isolated from insect frass collected in Thailand was found to represen... more ABSTRACT A strain of yeast isolated from insect frass collected in Thailand was found to represent a hitherto undescribed species of a basidiomycetous anamorphic genus Trichosporon. It is described as Trichosporon siamense. In the phylogenetic tree based on the D1/D2 region sequences of 26S rDNA, this yeast constitutes a cluster with several Q-9 having species of Trichosporon including T. otae and T. brassicae but is clearly differentiated from these species by 1.8% or more base substitutions. In the internal transcribed spacer region (ITS1 and ITS2), this species differs from T. scarabaeorum, the nearest species, by 6.5% base substitution.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2012
Two strains (NT29(T) and NT31(T)) of xylose-assimilating yeasts were obtained from soils collecte... more Two strains (NT29(T) and NT31(T)) of xylose-assimilating yeasts were obtained from soils collected in northern Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer region, the two strains were found to represent two novel ascomycete yeast species. Strain NT29(T) was assigned to the genus Candida belonging to the Pichia clade as a representative of Candida phayaonensis sp. nov.; the type strain is NT29(T) (=BCC 47634(T)=NBRC 108868(T)=CBS 12319(T)). Strain NT31(T) represented a novel Wickerhamomyces species, which was named Wickerhamomyces xylosica sp. nov.; the type strain is NT31(T) (=BCC 47635(T)=NBRC 108869(T)=CBS 12320(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2011
Five strains (RV5(T), RV140, R31(T), RS17 and RS28(T)) representing three novel anamorphic ascomy... more Five strains (RV5(T), RV140, R31(T), RS17 and RS28(T)) representing three novel anamorphic ascomycetous yeast species were isolated by membrane filtration from estuarine waters collected from a mangrove forest in Laem Son National Park, Ranong Province, Thailand, on different occasions. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and the internal transcribed spacer region and phylogenetic analysis, three strains were found to represent two novel Candida species. Two strains (RV5(T) and RV140) represented a single novel species, for which the name Candida laemsonensis sp. nov. is proposed. The type strain is RV5(T) (=BCC 35154(T) =NBRC 105873(T) =CBS 11419(T)). Strain R31(T) was assigned to a novel species that was named Candida andamanensis sp. nov. (type strain R31(T) =BCC 25965(T) =NBRC 103862(T) =CBS 10859(T)). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and phylogenetic analysis, strains RS17 and RS28(T) represented another novel species of Candida, for which the name Candida ranongensis sp. nov. is proposed. The type strain is RS28(T) (=BCC 25964(T) =NBRC 103861(T) =CBS 10861(T)).
Six yeast strains of a novel anamorphic yeast species were isolated from natural samples collecte... more Six yeast strains of a novel anamorphic yeast species were isolated from natural samples collected in Thailand (RV60 T and LYSM9), Taiwan (SC5L04 and GE19S05), and Japan (JCM 11058 and JCM 11059). Analysis of the D1/D2 domain of the large subunit rRNA gene sequences revealed that the sequences of five strains (LYSM9, SC5L04, GE19S05, JCM 11058, and JCM 11059) were identical and differed from the sequence of strain RV60 T by only one nucleotide substitution. The closest recognized species in terms of pairwise sequence similarity was Candida abiesophila, but the level of nucleotide substitution (14.2-14.3%) was sufficient to justify the description of a separate species. Phenotypic characteristics of the six strains were almost the same except for two strains from Japan, which showed the ability to ferment glucose and grew in the presence of 10% NaCl and 5% glucose while the others did not. The phenotypic characteristics of the six strains included proliferation by multilateral budding, absence of ascospores, arthrospores, and ballistospores, and negative Diazonium blue B and urease tests. The major ubiquinone was Q-7. On the basis of the above findings, the six strains were assigned to a single novel species of Candida, for which the name Candida asiatica sp. nov. is proposed. The type strain is RV60 T (BCC 25966 T = NBRC 103863 T = CBS 10863 T ).
Three yeast stains were isolated from two unknown fruits (strains DD2-22-1 T and SK44) and moss (... more Three yeast stains were isolated from two unknown fruits (strains DD2-22-1 T and SK44) and moss (strain in Thailand. Analysis of the D1/ D2 domain of the large subunit (LSU) rRNA gene sequences of the three strains revealed that they belonged to the same species. In terms of pairwise sequence similarity, Candida cf. glabrata UWO(PS) 98-110.4 and Candida nivariensis were the closest undescribed and recognized taxa, but the levels of nucleotide substitutions were 1.7-1.9% and 2.0-2.2%, respectively. The levels of nucleotide substitutions were sufficient to justify the description of a separate species of Candida. In the phylogenetic tree based on the D1/D2 domain of the LSU rRNA gene the three strains were placed in a separate branch in the Nakaseomyces clade with C. cf. glabrata UWO(PS)98-110.4, C. nivariensis, Candida glabrata, Candida bracarensis, Candida kungkrabaensis and Nakaseomyces delphensis. Phenotypic characteristics of the three strains were similar which included proliferation by multilateral budding, absence of ascospores, arthrospores or ballistospores; negative for Diazonium blue B and urease tests. The major ubiquinone was Q-6. On the basis of the above findings, the three strains were assigned to a single novel species of Candida, for which the name Candida uthaithanina sp. nov is proposed. The type strain is DD2-22-1 T (= BCC 29899 T = NBRC 104876 T = CBS 10932 T ).
Teleomorphic species of the genus Zygozyma and anamorphic species of the genus Myxozyma were exam... more Teleomorphic species of the genus Zygozyma and anamorphic species of the genus Myxozyma were examined phylogenetically. In phylogenetic trees based on 18S rRNA-, 26S rRNA-, mitochondrial small subunit rRNA-and EF-1α-gene sequences and concatenated sequences of the latter four regions, derived from the neighbor-joining method, the four species of the genus Zygozyma constituted four clusters, respectively, with low bootstrap values, indicating that all four species can be distinguished from one another at the generic level. The name of Limtongia was newly suggested for Zygozyma smithiae, and Limtongia smithiae was proposed as new combination.
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