Photometer

DTN-510K
SEMIAUTOMATIC CLINICAL
CHEMISTRY ANALYZER

USER MANUAL

NOTES:

June 2014
Revision 03
Software Version 1.3e
Dialab Produktion und Vertrieb von chemisch-technischen Produkten
und Laborinstrumenten Gesellschaft m.b.H.
A-2351 Wiener Neudorf, IZ-N Sd, Hondastrae, Objekt M55
Tel.: +43-2236-660910-0
Fax.: +43-2236-660910-30
E-Mail.: office@dialab.at

CONTENT
SYMBOLS ON INSTRUMENT .................................................................................................... 1
SAFETY PRECAUTIONS AND POTENTIAL HAZARDS ....................................................... 2
CHAPTER 1

BRIEF DESCRIPTION ................................................................................ 6

PRODUCT APPLICABILITY ............................................................................................................ 6

1.1
INSTALLATION .................................................................................................................. 7
1.1.1
Unpacking ................................................................................................................. 7
1.1.2
Instruments location .................................................................................................. 7
1.1.3
Power requirements .................................................................................................. 8
1.1.4
Connect the instrument to AC power ........................................................................ 8
1.1.5
Paper Installation...................................................................................................... 8
1.1.6
Flow-cell and cuvette system..................................................................................... 9
1.2
SYSTEM OVERVIEW .......................................................................................................... 9
1.2.1
Front View ................................................................................................................. 9
1.2.2
Keyboard Functions ................................................................................................ 10
1.2.3
Rear view................................................................................................................. 11
1.2.4
Flow-cell and cuvette .............................................................................................. 11
1.2.5
Lamp and sipper pump ............................................................................................ 12
CHAPTER 2

OPERATING PROCEDURES ...................................................................... 14

2.1
POWERING ON ................................................................................................................ 14
2.2
BASIC OPERATING .......................................................................................................... 15
2.2.1
Mouse ...................................................................................................................... 15
2.2.2
Keyboard ................................................................................................................. 15
2.2.3
Soft-keyboard .......................................................................................................... 15
2.3
MAIN MENU AND SYSTEM PARAMETERS SETTING ........................................................ 15
2.4
PROGRAMMING TEST PARAMETERS .............................................................................. 16
2.4.1
Modifying preprogram ............................................................................................ 16
2.4.2
Programming a new assays..................................................................................... 17
2.4.3
Calibration parameter ............................................................................................ 17
2.4.4
Control parameter ................................................................................................... 18
2.4.5
Selecting a mode ..................................................................................................... 19
2.4.6
Save the changed ..................................................................................................... 20
2.4.7
Return ...................................................................................................................... 20
2.5
PERFORM TESTS ............................................................................................................. 20
2.5.1
Test procedure menu................................................................................................ 20
2.5.2
Perform ................................................................................................................... 21
2.5.3
Changing test .......................................................................................................... 21
CHAPTER 3
3.1
3.2
3.3

CALIBRATION BY STANDARD ............................................................... 22

GENERAL ........................................................................................................................ 22
PROGRAM CALIBRATION ............................................................................................... 22
PERFORM LINEAR TESTS ............................................................................................... 22

3.4
3.5

PERFORM THE CALIBRATION OF NON LINEAR TESTS ................................................... 23

LIST CALIBRATION ......................................................................................................... 23

CHAPTER 4
4.1
4.2
4.3
4.4

CONTROL .................................................................................................... 24

GENERAL ........................................................................................................................ 24
PROGRAM CONTROLS .................................................................................................... 24
MEASUREMENT OF CONTROLS ...................................................................................... 24
LIST CONTROLS .............................................................................................................. 25

CHAPTER 5

REPORT ........................................................................................................ 26

5.1
SUMMARY ....................................................................................................................... 26
5.2 PRINT BY PATIENT ................................................................................................................ 26
5.3
PRINT BY TEST ................................................................................................................ 27
5.4
PRINT HISTORY RECORDS .............................................................................................. 28
CHAPTER 6

SHUT DOWN PROCEDURE ...................................................................... 28

CHAPTER 7

MAINTENANCE .......................................................................................... 29

7.1
GENERAL ........................................................................................................................ 29
7.2
CLEANING THE OUTSIDE................................................................................................ 29
7.3
CLEAN PROCEDURE FOR THE FLOW-CELL.................................................................... 29
7.3.1
Washing when Changing tests ................................................................................. 29
7.3.2
Washing twice a day ................................................................................................ 29
7.3.3
Shut down the system by the cshut down procedure ................................................ 30
7.4
LAMP REPLACEMENT ..................................................................................................... 30
7.5
SIPPER PUMP TUBE REPLACEMENT ............................................................................ 32
7.6
FUSE REPLACEMENT ...................................................................................................... 33
7.7 CLEAN THE HISTROY RESULTS ........................................................................................ 34
7.8
TROUBLE SHOOTING ...................................................................................................... 35
APPENDIX I: GENERAL SPECIFICATIONS ......................................................................... 36
APPENDIX II: SERIAL CONNECTION PROTOCOL ........................................................... 37
1.
2.
3.

SERIAL PORT STATE........................................................................................................ 37

MESSAGE FORMAT ......................................................................................................... 37
SEND OVER ..................................................................................................................... 37

Symbols on instrument
This symbol means that the labeled item is hot while the instrument
is in use. Dont touch the labeled item as you could be scalded.
The symbol is labeled on the lamp support of optic system.
This means that the labeled item could lead to personal injury
and/or damage to the analyzer.
The symbol is labeled beside the power outlet and some external
interface.
Cautions should be followed carefully to ensure your instrument
work correctly and to avoid unnecessary personal injury
The symbols for SERIAL NUMBER, The serial number shall be
after or below the symbol, adjacent to it.
The symbol means the product is in vitro diagnostic medical
device.
The symbol indicates the manufacturer and its address, after which
are shown its name and address.
The symbol indicates EU representatives of the manufacturer and
their addresses, after which are shown their names and addresses.
The symbol indicates biological pollution, marked in the part where
the instrument contacts the clinical reagent. The symbol appears in
black side and yellow background.

Symbols on packaging
The symbol means that the environment of instruments must be
water proof in course of transport and the instrument must be kept
in a dry environment.
The instrument should be handled with care in course of
transportation, not damaging it.
The package must stay upright. Do not tilt it.
The symbol means that the level piled up can't exceed 8 layers, as
not to damage instrument.
The symbol indicates temperature range of the analyzers during
storage and transportation.

Safety Precautions and Potential Hazards

General
Before you start installing and working with the analyzer, you should read the safety precautions
and regulations shown in this chapter.
Operator Qualification
Please note that the operation with Semi-auto Chemistry Analyzer should be carried out only by
the doctor or clinical inspector who has undergone necessary training provided by the sales agent.
Service Technician Qualification
To install, maintain and repair the instrument, a service technician has to be trained on the
instrument by the manufacturer or their representative. A service technician is also expected to be
familiar with the normal operation of the instrument as described in the Users manual and the
special operations as described in the service manual.
Electrical
To use analyser safely, pay attention to the following items:
To prevent the risk of electrical shock and/or damage to the instrument Operator should not open
the white cover of the instrument. Only authorized personnel, for example, service technicians,
may open the instrument to perform maintenance or repair.
Touching the main board when the power is on may cause severe injury or death. Any problem,
please ask for helps from your supplier.
Mechanical
There is no risk presented by the mechanical parts of the instrument when the instrument is closed.
If the covers of the instruments are removed, mechanical parts could cause personal injury or the
instrument may be damaged if the following advice is not being considered: DO NOT wear loose
garments or jewellery that could catch in mechanisms. DO NOT put your fingers/hands into the
pathway or any part while the analyzer is in operation. DO NOT attempt mechanical repair unless
the instrument is not in operation or OFF.
Lamp
The source lamp becomes extremely hot during operation; never touch the lamp when it is on!
Look directly into the light path of the lamp may cause eyes damage.
If the lamp needs to be changed, always switch off the lamp by switching off the instrument and
then wait until the lamp has cooled down.

Chemical
The operator is responsible for taking all necessary precautions against hazards associated with the
use of clinical laboratory chemicals. Specific recommendations for each reagent used with the
analyzer are normally found on the manufacturers package inserts or the on the product
information sheets for each chemical. Wipe up any reagent spillage on the instrument
immediately.
Biohazardus Materials
As with all vitro diagnostic equipment, patient samples and serum-based quality control (QC)
products are assayed on this system, as well as all waste from the waste container, should be
treated as the potentially biohazardous. All materials and mechanical components associated with
the sampling and waste system should be handled according to your facilitys biohazard procedure.
Use the personal protective equipment recommended by your facility when handling any of these
components. Detailed recommendations:
- Samples
Treat all samples as potentially biological pollutions and infectious. If any sample is spit on the
instrument, utilize the correct personal protective equipment (PPE- gloves, lab coat, etc..), wipe it
up immediately and clean the contaminated with a disinfectant.
- Waste solutions and solid waste
Avoid direct contact with waste solution and/or solid waste. Both should be handled as potentially
biohazardous.
Dispose of waste solution and/or solid waste according to the relevant governmental regulations.
Consult the reagent manufacturer for information on the concentrations of heavy metals and other
toxic constituents in each reagent.
- Biohazardous parts
Avoid direct contact with the sipper tubing and all parts of the sample flow path. Treat these areas
as potentially biohazardous and /or infectious
- Reagents
Avoid direct body-contact with reagents. Direct body-contact may result in irritation or damage to
your skin. Refer to the manufacturers reagent kit box and package inserts, or product information
sheets for specific instructions.
Avoid direct body-contact with cleaning solution. Direct body-contact amy result in skin irritation
or damage. Refer to the manufacturers kit box and package inserts, or product information sheets
for specific instructions.

ATTENTION! Cautions

Require for samples and reagents, calibrators and controls.

The DTN-510K is a reagent open system. But before the testing, the following items should be
noted first.
Generally, all the reagent and samples have to be stored and prepared as per the
manufactures instructions. Dialab do recommend that these reagents should from the
manufactures with valid product quality certificate and production permission legalized by
local government or from the valid distributors appointed Dialab in your country.
Before running a test, please check the following.
-

Check the expiration date of the material

Check if the reagent is stored properly as required. (E.g. cooled or frozen storage before
using)
Check for proper programming in parameter setting menu according to the specified
reagent instructions.
Check for volume required for each material.
Check for proper and calibrated pipette to prepare the solution.

Additional Precautions
- Flammables
Avoid using dangerous flammable material around the instrument.
- Accuracy/Precision of the Measured Results
For proper use of the instrument, measure control samples and monitor the instrument during the
operation.
An incorrectly measured result may lead to an error in diagnosis, there by posing a danger to the
patient.
Treat all reagents according to the manufacturers recommendations. Refer to the reagent kit box
and package inserts, or product information sheets for specific instructions.
Make sure that the sample/reagent mixture does not contain any blood clots, dust or other
insoluble contaminants. If insoluble contaminants are contained in the sample, correct measuring
values may not be obtained.

- Application
The instrument is designed for clinical chemistry test analysis using water-soluble samples and
reagents.
Please note that other types of analysis may not be applicable to the instrument.
- Operation and Maintenance
During operation and maintenance of the instrument, proceed according to the instructors and do
not touch any parts of the instrument other than those specified.
Never leave a reagents/sample mixture in the flow-cell for longer than necessary. Always clean the
flow-cell after a batch of measurement and keep the flow cell filled with distilled water when not
in use. Its recommend to rinse the flow cell with 30% dilute Alcohol after daily work and Weekly.
Verify the front covers closed while the instrument in operation.
Avoid touching the mechanism, such as the sipper mechanism inside the instrument, while the
instrument is operating. This may cause operation stop or damage the instrument.
When the instrument works for around one year, please clear the results data inside the instrument.
If there are too many results, it will affect the stability of the System and software.

Chapter 1

Brief Description

The DTN-510K is a universal photometric semi-auto chemistry analyzer. The advanced software
based on Windows CE gives the instrument friendly interfaces which meet the individual
requirements of clinical chemistry laboratory.

47 of the most commonly assays are stored in the flashing RAM of the DTN-510K,
which enables user can start working right away with the instrument. 130 of assays
can be programmed.

6 Analytical Modes:
Endpoint
Bichromatic
Liner regression
Multi-Calibration
Two-point
Kinetics

The user could connect the unit to the PC of clinic lab by RS232 interface.

Options: Keyboard

Power-off protection, real-time data saving.

Product applicability

Product applicability
This IVD instrument is a semiautomatic clinical chemistry analyser for quantitative In-Vitro
determination of analytes, medicines and drugs, such as:
Albumin, Alkaline Phosphatase, Ammonia, Pancreatic Amylase, Bile Acids, Direct Bilirubin,
Total Bilirubin, Calcium, Chloride, Total Cholesterol, HDL cholesterol, LDL cholesterol,
Cholinesterase, Creatine kinase, Creatine kinase MB, Bicarbonate, Copper, Creatinine, Ethanol,
Fructosamine, G6PDH, Gamma-GT, GLDH, Glucose, GOT (AST), GPT (ALT), HBDH, Total
Hemoglobin, HbA1c, Homocysteine, Hydroxybutyrate, Iron, Lactate, Lactate Dehydrogenase
(LDH), Lipase, Lithium, Magnesium, Free fatty acids, Phosphate, Potassium, Total protein,
Sodium, Triglycerides, Total iron binding capacity, Urea, Uric acid, Zinc, Alpha-1-antitrypsin,
Alpha-1-acid glycoprotein, Alpha-2-macroglobulin, Alpha-1-microglobulin, Apolipoprotein A1,
Apolipoprotein A1, Apolipoprotein B, Anti-streptolysin O, Antithrombin III, Beta-2-microglobulin,
C1-esterase inhibitor, Complement C3, Complement C4, Ceruloplasmin, C-reactive protein,
Cystatin C, Ferritin, Fibrinogen, Fibronectin, Haptoglobin, Immunoglobulin A, Immunoglobulin
D, Immunoglobulin G, Immunoglobulin M, Kappa light chains, Lambda light chains, Lipoprotein
(a ), Microalbumin, Myoglobin, Prealbumin, Rheumatoid factor, Transferrin, Carbamazepine,
Digoxin, Phenobarbital, Phenytoin, Valproic acid, Amphetamines, Barbiturates, Buprenorphine,
Benzodiazepines, Cocaine, Cotinine, Ecstasy, Methadone, Opiates, Cannabinoids, etc.

in human samples and has to be used in combination with In-Vitro diagnostical reagents and
calibration material.
This IVD product is intended for professional use only!

1.1

Installation

1.1.1

Unpacking
Carefully unpack the instrument, removing it from plastic bag. Keep the boxes and
packaging material until is assured that nothing is missing, no (internal) transport
damage and a proper working.

1.1.2

Instruments location
The DTN-510K should be located on a flat surface, which is not exposed to heavy
vibrations (i.e. centrifuges, etc.) and direct sunlight. The ventilation openings in the
bottom plate and at the rear of the instrument should not be blocked.
Note!

Environmental requirements
Ambient temperature (operating):
Relative humidity:
Transport and storage environment
Temperature:
Humidity:
Atmospheric Pressure:

0C ~ 40C
85%
-10C ~ 40C
85%
86kPa ~ 106kPa

1.1.3

Power requirements

1.1.4

a.c.110V~220V
50/60 Hz
80VA

Connect the instrument to AC power

Plug the power cord into the instrument. Plug the power cord into an AC electrical
wall outlet.

ATTENTION! Cautions

AC power outlet must be connected to ground.

Shut (Power) off the power if smog, special smell or special sounds
from the inside of the instrument, and contact your supplier.

Hold the connector when you pull off the power cord. Never pull the
cord directly.

1.1.5

Paper Installation

Remove the paper cover by pulling up at the top end of the cover.

Unroll about 10 inches (~25cm) of paper and place the roll on the table behind
the instrument.

Feed a cleanly cut edge of the paper from the back into the printer.

A ragged edge or wrinkled paper will be difficult to load and could cause a paper
jam.

Feed just over 1 inch of paper in and then press the feed paper key several times
to feed over 3 inches of paper. Drop the roll of paper into the printer paper well in
back.

Feed the paper through the paper slot of the cover, and then close the cover.

1.1.6

Flow-cell and cuvette system

Figure 1.1

Remove flow-cell cover

Insert the flow-cell or cuvette into the flow-cell holder

Make sure the surface with characters of the flow-cell is faced to user.

Note!
Keep the surface of the flow-cell or cuvette clean. Dont touch it by fingers.

1.2

System overview

1.2.1

Front View
1)

Printer cover & Pump cover: Open the cover, user could change the lamp or
maintain the pump.

2)

Flow-cell cover

3)

Sample tube bracket

4)

Sample tube

5)

Sample button

6)

Key board cover

7)

LCD Screen

8)

Paper feed button

9)

Rinse button

10)

Power-on indicator

Figure 1.2

1.2.2

Keyboard Functions

Figure 1.3
1)

Rinse on/off:

Pressing this button will switch on the sipper pump in continuous

mode. Pressing this button again will stop the pumping action at the end
of a complete cycle.

2)

Feed Paper:

Activates embedded printer

10

1.2.3

Rear view
11

10

Figure 1.4
1)
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)

1.2.4

Power switch
Fuse
AC power connector
Fan
Pump
Brightness
LAN port
USB port
SD card
RS-232 port
Drain port

Flow-cell and cuvette

The following sketches will help you to locate and identify the flow-cell parts.

Figure 1.5
11

Figure 1.6

1.2.5

Lamp and sipper pump

1)

Remove the flow-cell cover.

2)

The sketches of the lamp and the sipper pump are as show in the following figure1.7
and figure 1.8:

ATTENTION! Cautions
The source lamp becomes extremely hot during operation; never touch the
lamp when it is on! Look directly into the light path of the lamp may cause
eyes damage. If the lamp needs to be changed, always switch off the lamp by
switching off the instrument and then wait until the lamp has cooled down.

Figure 1.7

12

Figure 1.8

13

Chapter 2
2.1

Operating Procedures

Powering on
Turn on the instrument using the power switch on the rear panel. The screen will display the
following:

Screen 2.1 System initialization

The instrument will wait for two minutes to stabilize the lamp. The screen will display as
following:

Screen 2.2
Raise a test tube of distilled water up to the aspiration flow-cell sample tube and press the
Sample Button. After you hear the control unit valve cycle, remove the tube. The
Semi-auto chemistry analyzer will now read and save the absorbance reading for water for all
seven filters in the machine. If the absorbance reading is too high or too low, the following
screen will displayed: Absorbency deviation too big, Are you retry?

Screen 2.3
Select No, the system will ignore the deviation above and move to test menu. Select Yes,
and raise the test tube of water up to the aspiration tube, and press Rinse to rinse the
flow-cell. After a few seconds, press Rinse again to stop the rinse, and then press the
Sample Button. If the above screen still displayed, contact the supplier.
14

2.2

Basic operating

2.2.1

Mouse
The instrument has an USB port for a mouse.

2.2.2

Keyboard
Connect the ext. Keyboard to the instrument when the analyzer is powered off. You
can use the keyboard just like operating with a PC keyboard.

2.2.3

Soft-keyboard
Use mouse to click the icon (soft-key) to pop-up a soft-keyboard, (Screen 2.5 Soft
keyboard) and click the icon again, the soft-keyboard will be close
Click here when dragging

Screen 2.4 icon (soft-key)

2.3

Screen 2.5 Soft keyboard

Main menu and system parameters setting

After the instrument initialization, the screen will display the following:

Screen 2.6 Main menu

Click System,

Screen 2.7 System setting menu

15

1)

Setting time and date

2)

Printer Select

3)

Printer inside: If you select Printer Inside, the Printer type can not be selected.

Printer outside: If you select this, you need to select the printer type also.

Print instantly: If you select this, test result will be printed instantly.

Inputting the hospital name

Select Serial trans., the screen will display the following:

Screen 2.8 Serial transfer

The test result in DTN-510K can be transferred to a desktop PC through standard RS232
cable; the serial port of the PC should be set as following:
19200 baud, Even Parity, 8 bit of data and 1 bit of stop
Details of Protocol please see Appendix II.

2.4

Programming test parameters

The DTN-510K has a built-in menu of 130 assays. 47 assays of most commonly applied data
reduction formulae are pre-programmed.

2.4.1

Modifying preprogram
Click Setting in main menu. The screen will display the following:

Screen 2.9 Test survey menus

1) Click the button which will be modified, such as UA, The screen will display
the following:

16

Screen 2.10 Test Enter menus

Select Edit to modify the parameter of the test.

Select Print to print the parameter of the test.

2) Modifying parameters

Screen 2.11 Programming menu

3) Click Save button to save the modification, or click Exit to ignore.
2.4.2

Programming a new assays

1) Select a free button from the User defined test , then click the Edit button
2) Input parameters
3) Save the input

2.4.3

Calibration parameter
Select Calibration, Calibration setting window will be open.

Screen 2.12 Calibration setting menu

17

1)

Calibration for linear tests

2)

Calibration for no-linear tests

Num of std.: This parameter means that how many standards (with the
different concentration) will be used for calibration.

Repeat times: This parameter determines how many times will repeat for the
same concentration. Possible inputs are 1, 2 and 3.The results of multiple
measurements will be averaged by the system.
Select OK to confirm the selecting.
Select Exit to go back to above menu.

2.4.4

Num of std.: This parameter means that how many standards (with the
different concentration) will be used for calibration.
Repeat times: This parameter determines how many times will repeat for the
same concentration. The results of multiple measurements will be averaged by
the system.
Select OK to confirm the selecting.
Select Exit to go back to above menu with modify

Control parameter
Select Control for programming control. The follow window will appear:

Screen 2.13 Controls setting menu

a)
b)
c)
d)

Control 1 and control 2:

Mean value:
Minimum:
Batch number:

Two controls could be defined.

Input the mean value of the control.
Input the minimum of the control.
The different batches of a control will have
different values. Enter the batch number.
e) Select OK to confirm the programming. Select Exit to go back the above menu
without modify.

18

2.4.5

Selecting a mode
In the menu of the Programming (screen 2.10), click Mode. A window will
pop-up.

Screen 2.14 Measurement mode menus

Select Yes, the following screen appears:

Screen 2.15 Change mode menus

a) Endpoint
After a required incubation time, the reaction reaches its maximum color
development, or its Endpoint. The color of the reaction will remain stable and
constant for certain period of time. During this time a measurement in Absorbance
is taken.
b) Endpoint Bichromatic
Bichromatic is actually endpoint determination measured at two different
wavelengths. The advantage is that some unwanted interference due to the color of
the serum sample can be eliminated by subtracting the measurement done with the
second wavelength filters. This can be done when you select a sub-wavelength
in Parameter change menu. It was described in 2.4.1.
c) Endpoint determinations against a standard Curve
d) Endpoint determinations against a Multi-standard Curve
This is a normal endpoint determination, in which linearity may not be perfect
throughout the whole range that is interest. As a result it is calibration curve (a
number of standards) instead of a single standard.
e) Kinetic
Kinetic determinations are tests whose enzyme activity is measured continuously
over a certain period of time and finally referred to 1 minute.

19

f)

2.4.6

Two-point
After a pre-defined incubation time, two absorbance readings are taken at
different and programmed time interval. From the delta absorbance between the
two measurements the final result is calculated. Each of the measurements taken
is a mean of a large number of readings taken in quick succession.

Save the changed

After selecting the measure code, select Save change to save the selection. The
menu will change to the test enter menu. Select Enter to perform the test
procedure.

2.4.7

Return
Select Exit to go back to the Setting menu.

2.5

Perform tests
Select the test to be performed in the menu of test overview, and click OK to go to
the menu of test procedure.

2.5.1

Test procedure menu

The menu of test procedure is displayed as following:

Screen 2.16 Test procedure menus

1)
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)

Factor window:
ABS window:
Result window:
Control:
Calibration:
Print:
Change No.:
Time:
Temperature:
Lamp:
Wavelength:
Instruction window:
Exit:
Reaction window:

Factor
Display ABS of test
Test result
Perform the control test.
Perform the calibration test.
Print the test result.
Change the sequence number tested.
Display the time.
Display the temperature of the flow-cell.
Show the status of the lamp: On/Off.
Show the wavelength selected.
Display the instruction information.
Return back to main menu.
A curve will be displayed to represent the reaction.
20

2.5.2

Perform
Following the instructions displayed on the instruction window.

Screen 2.17
1) Instruction window:

Aspirating distilled water

Put a tube with distilled water under the sample tube and press Sample key
(figure 1.2).
The measure result will be displayed on the result window and on the reaction
window.
2) Instruction window:

Aspirating reagent blank or Aspirating Sample blank.

Hold the reagent blank or sample blank under the sample tube and press
Sample key. The programmed volume will be aspirated and measured. The
process and result of the measurement will be displayed.
4) Instruction window:

Aspirating Sample

Hold the sample under the sample tube and press Sample key. The programmed
volume will be aspirated and measured. The process and result of the measurement
will be displayed.

2.5.3

4) Calibration:

will be described in Chapter 3

5) Control:

will be described in Chapter 4

Changing test
When changing over from one test to another, washing of the flow-cell is required, to prevent
reagent carry over, causing wrong calibration and measurement result.
To wash the flow-cell:

Place a container of distilled water under the sample tube. Press Rinse key. The
aspiration/flow-cell system will be washed continuously now, until the Rinse key
is pressed again. Let the flushing continue for about 30 seconds.

21

Chapter 3
3.1

Calibration by standard

General
Certain tests require calibration by one or more standards. Calibration results are stored in the
flash memory of the analyzer and can be used again for future measurement.
Calibration can be programmed and subsequently done for different measurement mode:
Endpoint or Two-point. For Endpoint, the calibration could be with/without reagent blank
and/or sample blank. For Two-point, the calibration could only be with or without reagent
blank.
The calibration by standard should be performed depending on the stability of the reagents,
batch of the reagent, and type of test. The previous calibration data in the flash memory will
be overwritten by the new ones.
3 procedures should be performed for the calibration by standard:

3.2

Program calibrations
Measurement of calibrations
List calibrations

Program calibration
The calibration parameters could be programmed in the menu parameter modify, see the
screen 2.10. The setting was described in 2.4.2.

3.3

Perform linear tests

For linear tests, the relation between absorbance and concentration is a (linear) straight line.
This type of test is normally calibrated with one or more standard(s) of 1 concentration and
with or without reagent blank.
The calibration can be run at any time during a test run, after the test has been selected.
Select Calibration in menu of test procedure:
1)

Instruction window will appear:

Aspirating Standard #

Hold the standard sample # under the sample tube and press Sample key. The
programmed volume will be aspirated and measured. The process and result of the
measurement will be displayed.
2)

Instruction window:Aspirating Standard #, Repeat #

Follow the instruction on the instruction window.

Screen 3.1
22

3)

3.4

The result will be displayed on the result window, and the standard curve will be
displayed in same time. User can save and print the result

Perform the calibration of non linear tests

Non linear tests are those tests where the relation between absorbance and concentration is
not linear. These tests are normally calibrated on more than 1 standard of different
concentrations. The analyzer has a special arithmetic to link the calibration points to a
calibration curve. The measurements for patient or controls are then interpolated
automatically depending on the curve.
The calibration can be run at any time during a test run, after the test has been selected.
Select Cal. in menu of test procedure:
1)

Instruction window will appear:

Aspirating Standard #

Hold the standard sample # under the sample tube and press Sample key. The
programmed volume will be aspirated and measured. The process and result of the
measurement will be displayed.
2)

Instruction window:Aspirating Standard #, Repeat #

Follow the instruction on the instruction window.

3)

The result will be displayed on the result window, and the standard curve will be
displayed in same time. User can save and print the result.

Screen 3.2

3.5

List calibration
Select Calibration in Main menu, to list the calibrations. The list screen will be
displayed. Select test and click OK, following screen will be displayed:
1)
2)

Print the result by selecting the Print.

Select Exit to go back to the above menu.

Screen 3.3

Screen 3.3

23

Chapter 4
4.1

Control

General
The control program gives the possibility of storage and statistical evaluation of controls
measured on the instrument.
For all 130 tests programmed in the unit, 2 controls can be defined. The result of controls for
130 tests of 365 days could be stored automatically. 3 operating should be performed for the
controls:

4.2

Program controls
Measurement of controls
List statistics

Program controls
The control parameters could be programmed in the menu parameter modify, see the
screen 2.15. The setting was described in 2.4.3.

4.3

Measurement of controls
A control can be run at any time during a test run, after the test has been calibrated.

Screen 4.1
Select Controls in menu of Test procedure, a window will be opened as following:
1) Choice 1 if only measuring 1 control, and choice 2 if 2 controls will be measured.
Select OK to confirm the choice.
2)

Instruction window will display:

Aspirating Control #

Hold the control requested by analyzer under the sample tube and press Sample key.
The programmed volume will be aspirated and measured. The process and result of the
measurement will be displayed.
3)

The result will be added to the statistical memories automatically. The evaluation of
control results can be accessed through the main menu and described in 4.4.

24

4.4

List controls
Select Control in main menu, to list the statistics of controls. The list screen will be
displayed:

Screen 4.2
1) Select test and month, Click OK.
2) The statistic of the controls will be displayed on the screen:

Screen 4.3
3)
4)

Print the result by selecting the Print.

Select Exit to go back to the above menu.

25

Chapter 5
5.1

Report

Summary
In this part, three report styles are provided: By patient, By test and History, you can
select one style from three according your requirement.

Screen 5.1

5.2 Print by patient

In the state of screen 5.1, select By patient, a new window will be open.

Screen 5.2
Operation guide:
a) Update patient information
Select one patient, the correspondent line is activated. Input name, department, age
and sex, click Update, then the patient information is change into what you have
input.
b) Add a patient
If you want to add one or more new patients, you can click Add after the patient
information has been input. Each new patient will have a different patient NO. If you
want to test the patients sample, just change the present sample NO into the patient
NO.

26

c) Input patient No.

Input patient NO which need to be printed, patient NO can be input as following:
1.
2.
3.
4.
5.

3,6
1-5
1-4,5-6
3,4,5-7
If all patients need be selected, you can select the check box select all

Click Print, the report will be printed. If some item in the mode hasnt been tested, there will
have no correspondent data in the report.
d) Input no-chemistry item
Setting:
Directly inputting a no-chemistry item and result is also permitted, this item
will be printed in the General mode report. In the window of By patient, click Setting, a
window will be open as Screen 5.3. Test name, Unit, Normal (low) and Normal (high)
are all need to be input, and each cant be longer than eight characters. All the no-chemistry
items will be saved if the DTN-510K is closed correctly.

Screen 5.3
Edit:
In the window of By patient, select one patient line, and then click Setting.
In the Item edit window, input no-chemistry item result, this item will be printed in general
mode report.

5.3

Print by test
In the state of Screen 5.1, click By test, a new window will be open.
Select the Item to be printed, then click Print, all test result of selected item will be printed.
If you want print all items, just select the Select all check box.

27

5.4

Print history records

In the state of screen 5.1, click History, a new window will be open.

Screen 5.4
DTN-510K can store 3500 pieces of records at most. In the new window, drag the scroll bar,
you can see all test results of history.
During the operation of print, delete and data transfer, click "Select all" check box, you can
do the operation on all records. Input the order number of patient, you can do the operation
on selected records.
Patient No. can input as following:
1.
2.
3.
4.

3,6
1-5
1-4,5-6
3,4,5-7

Chapter 6

Shut down procedure

At the end of the working, you must perform the shut-down procedure by selecting Power
off in the Main menu.
1)
2)
3)
4)
5)

The system will store the parameters and the results of tests.
The screen will display aspirating distilled water.
Place a container of distilled water under the tube. Press Aspiration key , Rinse will
take few minutes
The screen will display Please switch off the instrument
Turn off the power.

ATTENTION! Cautions
Never power off the system without performing the shut down procedure. It
will cause that lost of the data and influence the measurement.

28

Chapter 7
7.1

Maintenance

General
The DTN-510K is a clinical semi-auto chemistry analyzer, which requires only a minimum
amount of maintenance.
Some general maintenance is necessary to keep the instrument in an optimal condition.

7.2

Cleaning the outside

Always keep the outside of the instrument clean and free of dust. You can wipe the outside
with a damp (not wet) cloth and a mild detergent.
Wipe the display screen with a soft, nonabrasive cloth.
ATTENTION! Cautions
Do not use any type solvent, oil, grease, silicone spray, or lubrication on
the instrument.

7.3

Clean procedure for the flow-cell

The inside of the flow-cell should be kept as clean as possible, to assure good and reliable
measurements.
To keep the inside clean, follow the following procedures:

7.3.1

Washing when Changing tests

When changing over one test to another, some washing of the flow-cell is required.
To wash the flow-cell:

Place a container of distilled water under the tube. Press Rinse key. The flow-cell
system will be washed continuously now, until the Rinse key is pressed again.
Let the flushing continue for about 2 minutes.

7.3.2

Washing twice a day

The following procedure is needed twice a day, to wash out those residues from the
wall of the flow-cell, which cause bad filling of the cell:

With distilled water and detergent. Let the detergent stay in the flow-cell for 2-3
minutes. The distilled water added some detergent is better. The ratio of the
detergent is depending on the detergent used. Normally a 2-5% solution is
adequate.
Rinse with distilled water without detergent for about 1 minute.

29

7.3.3

Shut down the system by the cshut down procedure

At the end of the working, you must perform the shut down procedure by selecting
Power off.
1) The system will store the parameters and the results of tests.
2) The screen will display distilled water.
3) Place a container of distilled water under the tube. Press Aspirate key, Rinsing
will take few minutes. Leaving the distilled water inside the flow-cell.
4) The screen will display Turn the power off
5) Turn off the power.

ATTENTION! Cautions
Never leave sample/regent inside the flow-cell for a long period of time.

7.4

Lamp replacement
Turn off the instrument, open the cover of lamp, pull out power supply wire of lamp, unscrew
the screw and then unscrew , take out the lamp from the holder.
The structure of lamp is showing in the following figure

Figure 7.4.1

&

Fixing screw
Adjusting screws

Its function is to fix the lamp to holder of lamp

Its function is to micro-adjust the location of
lamp and intensity of light (lamp)

Power source cable

It supplies power for lamp

30

1.

Replace a new lamp and install it to the holder, screw the screw but do not screw it
too firm; connect the lamp wire to power supply wire.

2.

Screw the screws & but do not screw it too firm.

3.

Turn on the instrument and enter the main menu, selectABS; enter setting window, set
Wavelength as 340nm and Reading as 240 secondinterval time 1 second. Then aspirate
distilled water to test.

Figure 7.4.2
AD

Figure 7.4.3
4.

Move the lamp forwards and backwards to maximize the tested AD value. The value is
in the small bracket (show as 3000), which is displayed on the screen.

5.

Use a screwdriver to fasten the screw (not too firm).

6.

Adjust screws and which enable the tested AD value is about 300016003000
is also permissible), then fasten the screw .

7.

The adjustment of lamp finished. Turn off the instrument normally.

8.

2 minutes later, turn on the instrument, when the initialization finished. Press the RINSE
to rinse the flow-cell with distilled water.

9.

Now the instrument is ready for test.

31

7.5

Sipper pump tube replacement

The whole view of sipper pump:
Clip Button
Adjust Screw

Pump

Clip Bar

Pump Tube
Motor

Figure 7.5.1
1. Unscrew the adjust screw off the clip bar.

Clip Bar

Figure 7.5.2
2. Open clip button, pump tube can be seen.

Clip Button
Pump Tube

Figure 7.5.3
32

3. Take out the pump tube, tear the belt, then the pump tube can be removed from the
white cuff.

Cable Tie
White Connector

Figure 7.5.4
4. Replace a new pump tube, tie the belt, and operate reservedly so that the
replacement can be completed.

7.6

Fuse replacement
The fuse is situated at the rear side of the analyzer. It is mounted in the fuse holder beside the
power switch. Pull out the fuse holder lid and the fuse can be replaced easily.
Replacement fuse should be the following rating:
Fuse rating: 250V, 3.15A

ATTENTION! Cautions
Never mount fuse of wrong rating!

33

7.7

Clean the histroy Results

To clean history Result, the procedure is in the following:

1. Please Click the Sys.setting. (Figure 7.7.1), then you can find Figure 7.7.2.

Figure 7.7.1

Figure 7.7.2
2. Click lock icon many times and then input password:991015, write down ID
(e.g.40050000), Hospital (hospital name), T value, A value (In Red Circle in Figure
7.7.2) .
3. Click the position (just right under ly of instantly) within red ring several times
to get into the back door of software & write down Pump Calibration, Pump, Front
board, Gain calibration, see the figure 7.7.3

Figure 7.7.3

4. Click the area within the red ring several times (Figure 4), which is right behind the
ID blank, to initialize the flashrom. When window "Do you want to initialize
flashrom" come up, click YES.

34

5.
6.
7.

8.

7.8

Figure 7.7.4
Wait for a few seconds, It pops up "Please turn the power off, turn off the
instrument again.
Two minutes later, turn on the instrument then the flashrom cleaning is done.
Enter the sys.setting window & input ID (e.g.40050000), Hospital (hospital name),
T value, A value & go into the back door of the software & input Pump speed, Pump,
Front board, Gain calibration, and Pump calibration & save them.
Turn off the instrument normally. The cleaning is finished.

Trouble shooting
1)

The analyzer could not power on

2)

The lamp couldnt turn on

3)

Not Print

4)

Non aspiration of sample

5)

The result of water blank is too high

6)
7)

Repeat result
Controls out of the target

---------------------------

Check the power cord

Check the fuse
Check AC power
Check the connector of the lamp
Change the lamp
Check the cable
Assure that printer powered on firstly.
Check the pinch pump
Tubing
Rinse the flow-cell
Check the lamp
Change distilled water
Rinse the flow-cell

---------

Reagent validity
Assure the program is correct.
The quality of controls
To test again by changing the
mode
Check the flow-cell and select a
new reagent and control
Liquid system blocked,
Rinse the tubing or change the tubing

--8)

The aspirate volume not stable

---

35

Appendix I: General Specifications

Net Weight:

8 kg

Dimension:

350mm(L)330mm(W)165mm(H)

Power supply:

A/C 110V~220V,50Hz/60Hz

Power consumption:

80VA

Fuses:

T3.15AL250V, 5x20mm

Operating condition:

0C ~40; RH 85%

Storage condition:

-10C to 40C; RH 85%

Display:

7 color LCD (640x240, 256 colors)

Measurement range:

0.000~2.500 Abs

Resolving power:

0.001 Abs (display), 0.0001 Abs (calculation)

Interference filters:

340nm, 405nm, 450nm, 500nm, 546nm, 578nm, and 670nm

Half band width:

10nm

Lamp:

Tungsten halogen lamp 6V/10W

Temperature control:

25C, 30C, 37C 0.1C and ambient temperature

Flow-cell:

30l quartz flow-cell.

36

Appendix II: Serial connection protocol

1.

Serial port state

Data is transmitted at 19200 baud,

With 1 stop bit
8 data bit and even parity

2.

Message format
The initial parameter must be the capital letter R, each line consists of 12 data in following
order:
Parameter

Possible values

Space mark

Initial parameter

Patient name

Character or NULL

Department

Character or NULL

Sex

Patient No.

Character or NULL
0 = Male
1 = Female
Character

Name of test

Character

Result

Character

Units of measurement

Character

Checksum

Character

Checksum = All of parameter + All of space mark

3.

Send over
DTN-510K sends a character string TRANSFER FINISH, when the task has been
completed.
Example:
R, Albert, 0, 43, 1, TP, 136.00, g/L, 0794
R, Bernhard, 1, 40, 2, TP, 147.00, g/L, 0765
R, Tony, 0, 6, 3, TP, 35.00, g/L, 069d
TRANSFER FINISH

37