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    Instructionset

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    api-341743184
    A micropipette is a precise tool used to transfer small volumes of liquid. It operates by drawing liquid into a disposable plastic tip when the plunger is depressed and released, and then dispensing the liquid when the plunger is fully depressed. The document provides step-by-step instructions on how to use a micropipette, including setting the desired volume, drawing up liquid into the tip, and dispensing the liquid into another container before disposing of the used tip. Micropipettes allow scientists to accurately measure and transfer volumes as small as 0.1 microliters.

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    Instructionset

    Uploaded by

    api-341743184
    100 views7 pages
    A micropipette is a precise tool used to transfer small volumes of liquid. It operates by drawing liquid into a disposable plastic tip when the plunger is depressed and released, and then dispensing the liquid when the plunger is fully depressed. The document provides step-by-step instructions on how to use a micropipette, including setting the desired volume, drawing up liquid into the tip, and dispensing the liquid into another container before disposing of the used tip. Micropipettes allow scientists to accurately measure and transfer volumes as small as 0.1 microliters.

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    A micropipette is a precise tool used to transfer small volumes of liquid. It operates by drawing liquid into a disposable plastic tip when the plunger is depressed and released, and then dispensing the liquid when the plunger is fully depressed. The document provides step-by-step instructions on how to use a micropipette, including setting the desired volume, drawing up liquid into the tip, and dispensing the liquid into another container before disposing of the used tip. Micropipettes allow scientists to accurately measure and transfer volumes as small as 0.1 microliters.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    100 views7 pages

    Instructionset

    Uploaded by

    api-341743184
    A micropipette is a precise tool used to transfer small volumes of liquid. It operates by drawing liquid into a disposable plastic tip when the plunger is depressed and released, and then dispensing the liquid when the plunger is fully depressed. The document provides step-by-step instructions on how to use a micropipette, including setting the desired volume, drawing up liquid into the tip, and dispensing the liquid into another container before disposing of the used tip. Micropipettes allow scientists to accurately measure and transfer volumes as small as 0.1 microliters.

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    How to Use a Micropipette

    What is a Micropipette?
    Scientists use a pipette when they need to deliver small volumes of a liquid in an accurate
    and precise manner. A pipette is a calibrated glass tube into which the liquid is drawn and
    released that can be relied upon to dispense volumes down to 0.1 mL. However, molecular
    biologists and chemists frequently work with volumes as small as 0.1 L, which calls for the
    use of a micropipette. A micropipette is a very fine pipette for measuring, transferring, or
    injecting very small quantities of liquid.
    Plastic tips are used as a medium to load and
    transfer the liquid sample. They allow the same
    device to be used for different samples without
    washing. They come in a number of different
    sizes and colors, depending on the micropipette
    they are used with and the volume to be
    dispensed.
    The most commonly used tips are:
    Large 200-1000L
    Medium 2-200L
    Small - <2L

    All micropipettes operate on the principle:


    A plunger is depressed by the thumb and as it is
    released, liquid is drawn into a disposable plastic
    tip. When the plunger is pressed again, the liquid
    is dispensed.

    Figure 1: A micropipette
    Source: http://www.di.uq.edu.au/sparqmicropipette

    STEP I: Set the volume


    1. Turn the centrally located rings clockwise to increase volume or
    counterclockwise to decrease volume until you reach the desired
    volume.
    The change in color indicates the position of the decimal point.

    P20: Maximum volume 20 l. Accurate between .5 l and 20 l.


    P200: Maximum volume 200 l. Accurate between 20 l and 200 l.
    P1000: Maximum volume 1000 l (1 ml). Accurate between 200 l and 1000 l.
    NOTE:

    Trying to dispense less than the lower value of the range will result in
    inaccurate measurements.

    Trying to dispense over the upper range will completely fill the tip and allow
    liquid to enter the body of the pipette.

    STEP II: Draw Up the Liquid

    1. Place a tip on the discharge end of the pipette.


    Pick the appropriate tip box and push the end of the micropipette into the
    tip and giving two sharp taps.
    NOTE:

    Be sure you use the proper size tip for each pipette.

    Always use a new tip for each different liquid.

    If sterile conditions are necessary, do not allow the pipet tip to touch any
    object including your hands.

    2. Depress the plunger until you feel the initial resistance and insert tip
    into the solution, just barely below the surface of the liquid.
    Do not go past Position 2 yet. This step determines the amount of liquid that
    will get transferred.

    Position 1 is where
    the pipette is at
    rest.

    Position 2 is reached by pushing


    Position 3 is reached by
    down on the plunger until initial pushing down from position 2
    resistance is met.
    and is the farthest point.

    NOTE:

    You should not reach the second stop when drawing liquid into the pipette.
    That is only to be reached when dispensing the liquid in the next step.

    If the solution you are pipetting is viscous, allow the pipet tip to fill to final
    volume before removing it from solution to avoid the presence of bubbles in
    the plastic tip which will result in an inaccurate volume.

    3. Carefully and slowly release plunger.


    NOTE:

    When withdrawing liquids with the pipette, always release the plunger slowly.
    This prevents liquid from rushing into the end of the pipette and clogging it
    up. This is especially important with large volume pipettes (200-1000 l).

    Never point a pipette up. This may cause liquid to run down into the pipette
    destroying it.

    STEP III: Dispense the Liquid

    4. Discharge the solution into the appropriate container by depressing


    plunger.
    This time, depress the plunger to the point of initial resistance (Position 2),
    wait one second, and then continue pressing the plunger as far as it will go
    (Position 3) in order to discharge the entire volume of solution.
    5. Remove tip by pressing down on the tip discarder.
    NOTE:

    Once used, tips are ejected into a sharps disposal bin using the tip eject
    button.

    Never touch the tip with your fingers, as this poses a contamination risk.

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