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YMC GENERAL CATALOG
1
Reversed-Phase
C18 Columns (ODS)
Types and characteristics of C18

packing materials 14
Guides for selecting C18 packing materials 15
YMC-Triart C18 16∼23
YMC-UltraHT series
YMC-UltraHT series 24,25
YMC-UltraHT Pro C18 26
YMC-UltraHT Hydrosphere C18 27
Pro series
Features of Pro series ODS columns 28∼31
YMC-Pack Pro C18 32∼35
Hydrosphere C18 36∼39
YMC-Pack Pro C18 RS 40∼43
YMC-Pack ODS series

YMC-Pack ODS-A 44,45
YMC-Pack ODS-AM 46,47
YMC-Pack ODS-AQ 48,49
YMC-Pack ODS-AL 50,51
,
J sphere ODS series
ODS-H80、M80、L80 52,53
YMC-Pack PolymerC18 54,55
13
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Types and characteristics of C18 packing materials
Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications. YMC Co., Ltd. offers packing materials with an impressive
assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety. We also offer
hybrid-silica based C18 packing materials that are widely applicable to a variety of fields, from microanalysis to large-scale isolation.
Types of C18 packing materials

Base Products
Hybrid silica YMC-Triart C18

YMC-UltraHT series
YMC-Pack Pro C18
Hydrosphere C18
Silica
YMC-Pack Pro C18 RS
Reversed-Phase C18 Columns
1 YMC-Pack ODS series

J’
sphere ODS series
Polymer YMC-Pack PolymerC18
Types and characteristics of C18 (ODS) packing materials

Pore Particle ODS
Usable
Product name size size Silanol pH range Characteristics Pages
（Å）（µm） C% treatment
YMC-Triart C18 2 ・Versatile hybrid silica based

（ ODS
ODS column
120 3 20 Yes 1.0 〜 12.0 16 〜 23
・Great chemical durability
5 ・Excellent peak shape in every conditions
）
UltraHT series ・2 µm-ODS column for Ultra-

fast LC method
Pro C18 120 2 16 26
・Superior separation of basic
compounds
Yes 2.0 〜 8.0
・Superior separation of
hydrophilic compounds
Hydrosphere C18 120 2 12 27
・Reduced analysis time while
maintaining resolution
Pro series 3 ・Processed with YMC Co., Ltd.’s
Pro C18 120 16 Yes advanced endcapping technology 32 〜 35
5 ・Superior separation of basic compounds
2.0 〜 8.0 ・Superior separation of
3
Hydrosphere C18 120 12 Yes 36 〜 39
・Can be used with 100% water
5 mobile phase
3 ・Highly durable ODS
Pro C18 RS 80 22 Yes 1.0 〜 10.0 ・Superior separation of basic compounds 40 〜 43
5 and hydrophobic compounds
YMC-Pack ODS series 3
120 5 17 ・Currently in use worldwide 44,45
10
ODS-A 5 Yes
200 12 ・ODS with wide pore size
10
・For separation of peptides 44,45
5
300 7 and proteins
10
3 2.0 〜 7.5
ODS-AM 120 17 Yes ・Outstanding lot-to-lot reproducibility 46,47
5
3
120 5 14
・Superior separation of
ODS-AQ 10 Yes 48,49
5
200 10
10
ODS-AL 120 5 17 No ・For separation utilizing residual silanol 50,51
J'sphere ODS series ODS-H80 80 4 22 Yes 1.0 〜 9.0 ・High carbon ODS
ODS-M80 80 4 14 Yes ・Medium carbon ODS 52,53
2.0 〜 7.5
ODS-L80 80 4 9 Yes ・Low carbon ODS
YMC-Pack PolymerC18 6 —
— — 2.0 〜 13.0 ・Polymer-based ODS 54,55
10 —
14
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Guides for selecting C18 packing materials
YMC Co., Ltd. offers twelve types of ODS columns, each with unique separation characteristics. The proper selection of packing
material is therefore a key factor in the establishment of efficient separation conditions. Selection of packing materials without sufficient
consideration of the separation characteristics may result in an expense of much time and effort in separating a target material.
YMC-Triart C18

YMC-Pack Pro C18 1
General organic compounds
YMC-Pack ODS-A
J’sphere ODS-M80
Low molecular Chemical structures differ YMC-Pack Pro C18 RS

weight compounds slightly among solutes J’sphere ODS-H80
Hydrophobicity differs YMC-Pack ODS-A (Wide-Pore)

greatly among solutes J’sphere ODS-L80
（ ODS
Hydrosphere C18
YMC-Pack Pro C18 RS
）
Difficult to separate YMC-Pack ODS-AL
using standard ODS J’sphere ODS-H80
J’sphere ODS-L80
YMC-Pack PolymerC18
YMC-Triart C18
YMC-Pack Pro C18
Hydrosphere C18
Basic compounds
YMC-Pack Pro C18 RS
YMC-Pack ODS-AM
YMC-Pack PolymerC18
YMC-Triart C18
YMC-Pack Pro C18
Hydrosphere C18
Coordination compounds
YMC-Pack Pro C18 RS
YMC-Pack ODS-AM
YMC-Pack PolymerC18
Hydrosphere C18
Sugars and glycosides YMC-Pack ODS-AQ
J’sphere ODS-L80
High molecular High molecular weight peptides YMC-Pack ODS-A (Wide-Pore)

weight compounds and oligonucleic acids
YMC-Triart C18
YMC-Pack Pro C18 RS
Alkaline mobile phase J’sphere ODS-H80
YMC-Pack PolymerC18
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Versatile Hybrid Silica Based HPLC Column
YMC-Triart C18
YMC-Triart C18 is a hybrid silica based ODS column emphasizing durability, low operating pressure, and excellent performance.
YMC-Triart C18 has been developed and is manufactured using state-of-the-art technologies that define materials, granulation
and surface modification.
Rugged, long lasting, hybrid particle technology

1
Integration of triple artistic technologies
Features Benefit
● Hybrid silica base U
sable over a wide pH range makes the column excellent for
▼
method development.
● Great chemical durability Long column lifetime over wide pH range.

▼

　Low cost per analysis.

（ ODS
● Lower back pressure A

bility to use on both older and newer instrumentation.
▼
）
● Excellent resolution R
ugged, first choice option for analysis of acids, bases and
▼
neutral compounds.
Specifications
Base ：organic/inorganic hybrid silica Carbon % ：20%
Stationary ：C18 (as USP-L1) Bonding ：polymeric type
Particle ：2 µm, 3 µm, 5 µm Endcapping ：multi-stage endcapping
Pore ：120 Å pH range ：pH 1 〜 12
YMC-Triart C18　－Integration of triple artistic technologies－

＜1＞Versatile hybrid material
YMC-Triart C18 is a multi-layer hybrid particle consisting of two types

of layers: a silica based layer (inorganic) and a hybrid polymer based
layer (organic). This layered structure contributes to Triart's combined
physical durability and chromatographic performance. Particle
synthesis by
Microreaction
Versatile
inorganic-silane layer hybrid silica
material
Multi-stage
endcapping
Image structure
for hybrid-silica gel
organic-silane layer
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YMC-Triart C18　－Integration of triple artistic technologies－
＜2＞New granulation process by microreactor technology
Homogeneous and uniform particle Narrow pore distribution
Triart C18 4500
4000
Hybrid particle for Triart
3500
3000
d Vp/d logD
Silica particle for
2500 conventional product
2000

1500
Hybrid particle for 1
competitor's product
1000
500
0
0 50 100 150 200 250 300 350
Pore size (Å)
Use of microreactor technology produces homogeneous and uniform YMC-Triart C18 has a very narrow pore size distribution
particles that results in low operating pressure and very reproducible that enables narrow and reproducible peak shapes for
surface modification making for the ultimate in column-to-column and analytes.
lot-to-lot reproducibility.
（ ODS
＜3＞Surface modification technology
）
Multi-stage endcapping
Endcapping methods employed for production of conventional products typically employ a single endcapping compound in one
chemical step to "neutralize" highly reactive silanols. Unfortunately, highly reactive silanols are easily hydrolyzed and contribute to
poor durability. Additionally, low reactive silanols endcap stubbornly, producing a surface modification that is often non-reproducible,
and leading to peak tailing. YMC-Triart C18 solves this problem by using an innovative, newly developed "measured multi-stage/multi-
compound surface modification" technology which results in the thorough endcapping of all silanols that are accessible to analytes.
Reaction image for "multi-stage endcapping" Evaluation of silanol activity

mAU
60 Triart C18
Before
EC
1
STEP
2
STEP
2
1
40
3
20
Q090928C
0
0.0 5.0 10.0 15.0 min
mAU
Silanol group 60 Endcapping
by traditional method
Endcapping (EC) group
40 Peak tailing for basic compounds
3
3
1
20
STEP
2
Q090928E
0
0.0 5.0 10.0 15.0 min
Column ：5 µm, 150×3.0 mmI.D.

Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)
Flow rate ：0.425 mL/min
Temperature ：40 ℃
Detection ：UV at 235 nm
Sample ：1. Chlorpheniramine　　2. Dextromethorphan
3. Propyl p-hydroxybenzoate
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YMC-Triart C18
Quality Control　－Excellent reproducibility－

Packing Material YMC-Triart C18 exhibits excellent lot-to-lot reproducibility for all types of compounds including difficult to
chromatograph molecules that exhibit basic and coordination compound behavior.
Basic compounds Coordination compounds
2 2
1 1
3
1 Lot. E Lot. E
Lot. D Lot. D
Lot. C Lot. C
Lot. B Lot. B
Lot. A Lot. A
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min 0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 min
J091201X
J091201Y
1. Chlorpheniramine 2. Dextromethorphan 1. Hinokitiol

3. Propyl p-hydroxybenzoate 2. Methyl benzoate
（ ODS
Column ：5 µm, 150×3.0 mmI.D. Column ：5 µm, 150×3.0 mmI.D.

Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35) Eluent ：acetonitrile/0.1% H3PO4 (40/60)
Flow rate ：0.425 mL/min Flow rate ：0.425 mL/min
Temperature ：40 ℃ Temperature ：40 ℃
）
Detection ：UV at 235 nm Detection ：UV at 254 nm
Packed Column Rigid control of theoretical plate number (N) and tailing factor (Tf) are exhibited for each YMC-Triart C18 packed
column.
20000 2.5
Theoretical plate number

15000 2.0
Tailing factor
10000 1.5
Tailing factor
5000 1.0
Eluent ：acetonitrile/water (60/40)
0
10 20 30 40 50
0.5 Temperature ：ambient
Column number Sample ：butyl benzoate
Low back-pressure　－Free from pressure problem－ Standard ODS　－Best balance hydrophobicity－

Column pressure and solvents Comparison of separation selectivity
0.180
Hydrogen-bonding capacity α (caffeine/benzene)
20
Triart C18, MeOH/W
18 0.160
Triart C18, ACN/W
Column pressure (MPa)
16
Pro C18, MeOH/W
14 Hydrosphere C18 Develosil C30-UG
Pro C18, ACN/W 0.140
12 Atlantis T3
CAPCELL PAK C18 AQ Standard ODS
10
The most versatile
0.120
8 CAPCELL PAK C18 MGIII columns as first-choice.
Gemini C18
6 Pro C18
0.100 Unison UK-C18 Inertsil ODS-4
4 Hypersil GOLD CAPCELL PAK C18 MGII
Gemini NX-C18
SunFire Inertsil ODS-3 Develosil ODS-SR
2 XTerra MS C18 XBridge C18
Mightysil RP-18 C18
Eternity-C18
L-column ODS
0
0 10 20 30 40 50 60 70 80 90 100
0.080 Triart C18
Cadenza CD-C18
TSKgel ODS-100S L-column2 ODS
Organic solvent rate (%) Pro C18 RS
0.060
Eluent : MeOH/W (80/20) [amylbenzene], MeOH/W (30/70) [caffeine, benzene]

0.040
Column ：5 µm, 150×4.6 mmI.D. 2.00 4.00 6.00 8.00 10.00 12.00 14.00
Eluent ：acetonitrile/water or methanol/water Hydrophobicity k’ (amylbenzene)
(S090805A)

Conventional hybrid silica based ODS columns tend to be less

YMC-Triart C18 is designed for use in all conditions. Even with hydrophobic than silica based columns. YMC-Triart C18 has a
higher viscosity methanol, YMC-Triart C18 can operate with favorable carbon balance and may be used as a "versatile first-
lower pressure (typically 30% less than conventional columns). choice" method development column.
18
第01章.indd 18 11.1.28 10:39:17 AM

Great durability　－Wide usable pH range and long lifetime－
Durability in high pH
Phosphate buffer (pH 11.5, 40 ℃) Triethylamine (pH 11.5, 40 ℃)

120% 120%
YMC-Triart C18
Rate of theoretical plate number

100%
YMC-Triart C18 100%
80% 80%
60% 60%
40% Hybrid-silica based C18 40%

Silica based C18
Silica based C18
20% 20%

1
0% 0%
0 10 20 30 40 50 0 50 100 150 200 250 300
Time (hr) Time (hr)
Column ：5 µm, 150×4.6 mmI.D. Column ：5 µm, 150×4.6 mmI.D.

Eluent ：50 mM K2HPO4-K3PO4 (pH 11.5)/methanol (90/10) Eluent ：50 mM triethylamine (pH 11.5)/methanol (90/10)
Sample ：benzyl alcohol Sample ：benzyl alcohol
Durability in high temperature
pH 6.9, 70 ℃ pH 1, 70 ℃
（ ODS
120% 120%
YMC-Triart C18
）
100% 100%
YMC-Triart C18
Rate of retention time
Silica based C18

80% 80%
Silica based C18

60% alkaline-resistant type 60% Hybrid-silica based C18
40% Silica based C18 40%
20% 20% The columns are kept in acetonitrile/water/TFA (10/90/1), 70 °C,

and tested for performance every 20 hours.
0% 0%
0 100 200 300 400 500 600 700 0 20 40 60 80 100 120
Time (hr) Time (hr)
Column ：5 µm, 50×2.0 mmI.D. Test conditions Column ：5 µm, 50×2.0 mmI.D.
Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (90/10) Eluent ：acetonitrile/water (60/40)
Sample ：phenol Sample ：butyl benzoate
YMC-Triart C18 shows great chemical stability provided by newly developed hybrid silica. Even in high pH or high temperature
conditions, the lifetime of YMC-Triart C18 is more than 10 times greater than conventional ODS columns.
Reduced column bleed　－Operate at high sensitivity－

Bleed test by LC/MS
1.2E+09 Scan ES+
TIC − Triart C18
− No column
8.0E+08

Eluent ：A) water/formic acid (100/0.1)
4.0E+08 B) acetonitrile/formic acid (100/0.1)
5% B (0-1 min), 5-100% B (1-5 min), 100% B (5-10 min),
100-5% B (10-10.1 min), 5% B (10.1-12.5 min)
0.0E+00 Temperature ：40 ℃
0 2 4 6 8 10 12 min Detection ：ESI positive, TIC (Mass Range: 50-1000)
Bleeding of stationary phase can often contribute to high background signals when employing MS and other hyphenated detection
techniques. In the example cited above, use of a 50×2.0 mmI.D. YMC-Triart C18 column shows no contribution to the signal observed
by the MS positive total ion current (TIC) trace. Such performance allows for low limits of detection (high S/N ratio) for MS and other
detection techniques. The inherent hybrid particle strength is also superior to conventional silica materials for detection techniques
that are sensitive to particle degradation.
19
第01章.indd 19 11.1.28 10:39:21 AM

YMC-Triart C18
Excellent resolution　－Without adsorption and tailing－

Analysis of basic compounds
Ingredients in a cough/cold medication
Cl O
1. CH3 2. CH3
1
2 YMC-Triart C18 N
5 µm, 150×3.0 mmI.D. H3C
3
N
N
H
1 Q090928C
CH3
Chlorpheniramine Dextromethorphan
1
Silica based C18 3. OH
2 Brand D5
3
5 µm, 150×4.6 mmI.D. O
H3C
O
Q090821C
Propyl p-hydroxybenzoate
Silica based C18

1
Brand N1 Column ：150×3.0 mmI.D. or 150×4.6 mmI.D.
3
2
3 µm, 150×3.0 mmI.D. Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35)
Flow rate ：0.425 mL/min for 3.0 mmI.D.
1.0 mL/min for 4.6 mmI.D.
J091111K
1 2
3 Hybrid-silica based C18
Brand I8
（ ODS
5 µm, 150×4.6 mmI.D.
Q090123F YMC-Triart C18's innovative surface modification

technology results in excellent peak shapes even for the
）
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min basic compounds that often exhibit tailing shapes on
conventional silica and hybrid silica based ODS columns.
Analysis of coordination compounds Hinokitiol

CH3
1. CH3 2. O O
CH3
1 2 YMC-Triart C18
5 µm, 150×3.0 mmI.D. OH
O
Q090925G Hinokitiol Methyl benzoate
2 Silica based C18 Column ：150×3.0 mmI.D. or 150×4.6 mmI.D.

1 Brand N1 Eluent ：acetonitrile/0.1% H3PO4 (40/60)
3 µm, 150×3.0 mmI.D.
J091110B 1.0 mL/min for 4.6 mmI.D.
2 Detection ：UV at 254 nm
Hybrid-silica based C18
Brand I8
5 µm, 150×4.6 mmI.D.
1
Q090120A YMC-Triart C18 has an extremely low level of metal
impurities, much lower than conventional products.
0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 min YMC-Triart C18 is able to provide excellent peak shape for
coordination compounds.
Analysis of acidic compounds Organic acid

1. Formic acid
1
YMC-Triart C18 2. Acetic acid
5 µm, 150×3.0 mmI.D. 3. Propionic acid
2
Column ：150×3.0 mmI.D. or 150×4.6 mmI.D.
3 Eluent ：acetonitrile/0.1% H3PO4 (5/95)
1.0 mL/min for 4.6 mmI.D.
Q090605C Temperature ：37 ℃
1 Detection ：UV at 210 nm
Hybrid-silica based C18
2
Brand G4
5 µm, 150×4.6 mmI.D.
YMC-Triart C18 is synthesized utilizing methodology
3 borrowed from microreactor technology. This synthesis
technique allows for a reduction of impurities that contribute
Q090717A to peak tailing during the analysis of some types of acidic
compounds.
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 min
20
第01章.indd 20 11.1.28 10:39:24 AM

Excellent versatility　－Buffer independency－
Comparison of clemastine analysis with conventional columns
10 mM phosphate buffer (pH 6.7)/organic solvent 10 mM CH3COONH4/organic solvent

methanol acetonitrile methanol acetonitrile
YMC-Triart C18
50×2.0 mmI.D.
Tf 0.97 Tf 1.11 Tf 1.03 Tf 1.16
F090818H07 F090818I07 F090818F07 F090817G07
Silica based C18

Brand C1
50×2.1 mmI.D.
Tf 1.21 Tf 1.56 Tf 1.13 Tf 1.43 1
F090727H07 F090727I07 F090727F07 F090803G07
Silica based C18

Brand D5 Tf 0.93 Tf 2.83 Tf 1.05 Tf 2.21
50×2.0 mmI.D. F090717H07 F090717I07 F090714F07 F090714G07
Silica based C18

Brand I6 Tf 1.03 Tf 3.87 Tf 1.13 Tf 1.88
50×2.1 mmI.D. F090722H07 F090722I07 F090722F07 F090722G07
Hybrid-silica
based C18 Tf 1.14 Tf 1.88 Tf 1.22 Tf 1.78
Brand I8
（ ODS
F090731H07 F090731I07 F090731F07 F090731G07
50×2.1 mmI.D.
10.0 11.0 12.0 min 6.0 7.0 8.0 min 8.0 9.0 10.0 min 6.0 7.0 8.0 min
）
H3C Column ：5 µm, 50×2.0 mmI.D. or 50×2.1 mmI.D.
N O
CH3 Eluent ：A) 10 mM KH2PO4-K2HPO4 (pH 6.7) or 10 mM CH3COONH4 (pH 6.7)
B) methanol or acetonitrile
5-90% B (0-10 min), 90% B (10-15 min)
Cl Flow rate ：0.2 mL/min
Clemastine
Clemastine is a well known basic compound which can easily tail on conventional ODS columns. YMC-Triart C18 can separate
clemastine sharply using many buffer/solvent combinations.
Improvement of loadability　－Minimizing strong solvent/sample loading effects－

Influence on peak shapes by injection volume
solvent for sample injection volume
1
2
YMC-Triart C18 acetonitrile 1.0 µL
1.5 µL
2.0 µL
3.0 µL
1 Hybrid-silica based C18

2 Column ：5 µm, 50×2.0 mmI.D. or 2.1 mmI.D.
Eluent ：A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5% B（0-0.5 min） , 5-100% B (0.5-2.5 min)
1 2 Silica based C18
1. 2.
Peak anomaly at 1.5 µL OCH2CHCH2NHCH(CH3)2 H3CO OCH3
CN CH3
OH
H3CO C (CH2)3NCH2CH2 OCH3
CH(CH3)2
1.5 2.0 2.5 min
Propranolol (50 µg/mL) Verapamil (50 µg/mL)
YMC-Triart C18 can tolerate larger injection volumes of samples containing strong solvents (e.g., acetonitrile) while allowing for
better peak shape than traditional columns. This can be important for difficult to solubilize samples that need higher concentrations of
organic solvent for solubilization prior to analysis.
21
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YMC-Triart C18
Fast analysis of paraquat and diquat
YMC-Triart C18 5 µm, 150×2.0 mmI.D. YMC-Triart C18 5 µm, 150×2.0 mmI.D.
mAU mAU
10%B, 0.2 mL/min, 5.7 MPa 1 5%B, 0.2 mL/min, 5.6 MPa
25 2 25
20 20
Insufficient Eluent optimization Baseline separation, 1
1 15
separation 15
But long analysis time 2
10 10
5 5
0 0
R091126J R091127C
0 2 4 6 8 10 12 14 min 0 5 10 15 20 25 min
Downsizing
YMC-Triart C18 3 µm, 50×2.0 mmI.D.

1. 2. 1
mAU
N+ N+ 2
·2Br− H3C N+ N+ CH3 25
·2Cl− 5%B, 0.4 mL/min,
（ ODS
20 12.2 MPa
Diquat Paraquat
80% reduction in run time
15
）
10
Eluent ：A) water/HFBA* (100/0.1)
5
B) acetonitrile/HFBA* (100/0.1)
Temperature ：37 ℃ 0
Detection ：UV at 290 nm R091201B
Injection ：1 µL (0.1 mg/mL) 0 1 2 3 4 5 min
*heptafluorobutyric acid
Application （Q091110E）
Anthocyanin
1. Delphinidin-3-O-galactoside
2. Delphinidin-3-O-glucoside
3. Cyanidin-3-O-galactoside
mAU 1 4. Delphinidin-3-O-arabinoside
2 5. Cyanidin-3-O-glucoside
6. Petunidin-3-O-galactoside
7. Cyanidin-3-O-arabinoside
100 8. Petunidin-3-O-glucoside
9. Peonidin-3-O-galactoside
10. Petunidin-3-O-arabinoside
11. Delphinidin
12. Peonidin-3-O-glucoside
80 4 13. Malvidin-3-O-galactoside
14. Peonidin-3-O-arabinoside
15. Malvidin-3-O-glucoside
16. Malvidin-3-O-arabinoside
5
17. Cyanidin
60 18. Petunidin
3 19. Peonidin
8 20. Malvidin
7
40
15
6
16
12 17
20 Column ：YMC-Triart C18 (5 µm, 120 Å)
10
11 13 18 20 250×4.6 mmI.D.
9 Eluent ：A) water/formic acid (90/10)
14 19 B) acetonitrile/methanol/water/formic acid
0 　 (22.5/22.5/40/10)
20-28% B (0-30 min), 28-70% B (30-40 min),
100% B (40-45 min)
0 5 10 15 20 25 30 35 40 min Flow rate ：1.0 mL/min
Detection ：UV/VIS at 535 nm
Sample ：commercial bilberry powder (1.25 mg/mL)
22
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Application （R091009C）
Peptide
mAU 1. Oxytocin (MW 1,007)
3
100 2. Met-Enkephalin (MW 574)
6
3. Leu-Enkephalin (MW 556)
1
4. Neurotensin (MW 1,673)
75 4
2 5. γ-Endorphin (MW 1,859)
6. β-Endorphin (MW 3,465)
50
150×2.0 mmI.D.
25
Eluent ：A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
0
20-45% B (0-25 min)
0 5 10 15 20 25 min

1
Application （S100121A）
LC/MS analysis for Triazolam and Etizolam

1. H3C
2. H3C
High resolution extracted chromatograms for［M+H］
+ N N
Intens . N N
x104 Mixture 100 ppb N N
6 2 S
5 Cl N H3C N
4 Cl
1 Cl
（ ODS
Triazolam (MW 343.22) Etizolam (MW 342.85)
2

50×2.0 mmI.D.
）
0 Eluent ：A) 10 mM HCOOH, B) acetonitrile
0 2 4 6 8 10 12 14 Time [min] 25-50% B (0-10 min), 25% B (10-15 min)
100ng_mL_RB4_01_2291.d: EIC 343.051±0.01 +All MS Flow rate ：0.2 mL/min
100ng_mL_RB4_01_2291.d: EIC 343.078±0.01 +All MS
Courtesy of J.Watanabe, Bruker Daltonics K. K. Detection ：ESI, positive mode (Bruker Daltonics micrOTOF)
Injection ：5 µL
Ordering information
Analytical columns Semi-preparative columns

Particle size Pore size Column size Particle size Pore size Column size
Product number Product number
（µm）（Å） inner diameter×length（mm）（µm）（Å） inner diameter×length（mm）
S-3 120 2.0× 20 TA12S03-0202WT S-5 120 10×150 TA12S05-1510WT
2.0× 30 TA12S03-0302WT 10×250 TA12S05-2510WT
2.0× 50 TA12S03-0502WT 20×100 TA12S05-1020WT
2.0× 75 TA12S03-L502WT 20×150 TA12S05-1520WT
2.0×100 TA12S03-1002WT 20×250 TA12S05-2520WT
2.0×150 TA12S03-1502WT
3.0× 50 TA12S03-0503WT Semi-preparative columns YMC-Actus Triart C18
3.0×100 TA12S03-1003WT S-5 20× 50 TA12S05-0520WX
120
3.0×150 TA12S03-1503WT 20×100 TA12S05-1020WX
4.6× 35 TA12S03-H546WT 30× 50 TA12S05-0530WX
4.6× 50 TA12S03-0546WT 30× 75 TA12S05-L530WX
4.6× 75 TA12S03-L546WT 30×100 TA12S05-1030WX
4.6×100 TA12S03-1046WT See P138-141 for details of YMC-Actus series.
4.6×150 TA12S03-1546WT
S-5 120 2.0× 20 TA12S05-0202WT Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)
2.0× 30 TA12S05-0302WT S-3 120 2.0×10 TA12S03-0102CC
2.0× 50 TA12S05-0502WT 4.0×23 TA12S03-G304CC
2.0× 75 TA12S05-L502WT S-5 120 2.0×10 TA12S05-0102CC
2.0×100 TA12S05-1002WT 4.0×23 TA12S05-G304CC
2.0×150 TA12S05-1502WT A cartridge holder will need to be purchased separately before using this product for the first time.
3.0× 50 TA12S05-0503WT
3.0× 75 TA12S05-L503WT Cartridge holder
3.0×100 TA12S05-1003WT Semi-micro guard cartridge holder
3.0×150 TA12S05-1503WT XPCHSMW
4.6× 50 TA12S05-0546WT
(inner diameter: 1.0, 1.5, or 2.0 mm)
4.6× 75 TA12S05-L546WT Cartridge holder
XPCHW
4.6×100 TA12S05-1046WT (inner diameter: 4.0 mm)
4.6×150 TA12S05-1546WT
4.6×250 TA12S05-2546WT Guard columns
Please inquire for 2 µm columns. 10×30 TA12S05-0310WTG
S-5 120
20×50 TA12S05-0520WTG
23
第01章.indd 23 11.1.28 10:39:40 AM

Ultra-Fast LC column
YMC-UltraHT
YMC-UltraHT series is a C18 bonded-phase based on standard ODS, and Hydrosphere C18 for hydrophilic
highly efficient 2 µm spherical silica particles. YMC- compounds. Both of them show the same selectivity
UltraHT series columns are specifically designed for and superior peak shapes as 3 µm and 5 µm Pro series,
high-speed and high throughput analysis in various fields allowing for an easy method transfer from conventional
such as pharmaceuticals, foods, and environmentals. HPLC to ultra-fast HPLC without changing elution
YMC-UltraHT series has two products. Pro C18 as our conditions.
2 µm ODS column ideal for Ultra-Fast LC
Features
● Superior column performance at higher flow rate and higher pressure
● Reduced analysis time while maintaining excellent resolution
（ ODS
● Excellent resolution with back pressure less than that of sub-2 µm

● Applicable to both conventional HPLC and specific ultra pressure LC system
）
● Same selectivity and superior peak shapes as 3 µm and 5 µm Pro series

●S
imple method transfer from conventional HPLC on Pro series; no need to
change elution conditions
Characteristics of 2 µm packing material
Van Deemter curves Column pressure

0.020 80
Optimum flow rate
for 5 µm Optimum flow rate for 2 µm Optimum flow rate for 2 µm Brand I7 1.7 µm
(0.2 mL/min for 2 mmI.D.) 70
(0.3-0.8 mL/min for 2 mmI.D.) (0.3-0.8 mL/min for 2 mmI.D.)
YMC 5 µm Brand L1 1.8 µm
Column pressure (MPa)
0.015 60
HETP (mm/plates)
50
YMC 3 µm
0.010
Brand L1 1.8 µm 40 YMC 2 µm
applicable
YMC 2 µm 30 pressure range
for conventional LC YMC 3 µm
0.005 20
Brand I7 1.7 µm
10 YMC 5 µm
0.000 0
0 1 2 3 4 5 6 7 8 9 10 11 0 1 2 3 4 5 6 7 8 9 10 11
Interstitial linear velocity (mm/sec) Interstitial linear velocity (mm/sec)
YMC-UltraHT series shows nearly the same efficiency as sub-2 Column : 50×2.0 or 2.1 mmI.D.
µm columns for ultra fast LC system and reduces the column Eluent : acetonitrile/water (60/40)
Temperature : 25 ℃
pressure significantly. Sample : Butyl benzoate
YMC-UltraHT series
Pro C18 Hydrosphere C18
Particle Size (µm) 2 2
Pore Size (Å) 120 120
Specific Surface area (m2/g) 330 330
Carbon Content 16% 12%
Bonding Type Monomeric Monomeric
Available pH Range 2-8 2-8
Characteristics Standard type For polar analytes
24
第01章.indd 24 11.1.28 10:39:43 AM

Ultra Fast analysis for flavonoids
conventional LC method：YMC-Pack C18

150×4.6 mmI.D. 5 µm
tR(7) 24.3 min

1.0 mL/min N(7) 77,000 /m
5 µL injection
8.5 MPa

1
Triplear Ultra-fast LC method：YMC-UltraHT C18
line city 50×2.0 mmI.D. 2 µm
Velo
90% Decrease in analysis time Flavonoids

90% Increase in efficiency 1. Myricetin
2. Quercetin
3. Apigenin
tR(7) 2.7 min 4. Kaempferol
5. Baicalein
N(7) 144,000 /m 6. Chrysin
7. Acacetin
0.6 mL/min
（ ODS
1 µL injection
30 MPa Eluent : acetonitrile/water/formic acid
(35/65/0.1)
）
Detection : UV at 260 nm
This example shows the method transfer from conventional HPLC method to Ultra-fast LC method using a YMC-UltraHT column. Since
the column efficiency is not decreased even when the flow rate is 3 times as fast as the conventional condition, it can be shorten
analysis time by 90% without losing the separation characteristics.
Applicable for conventional LC system
50×3 mmI.D. 50×2 mmI.D.

Conventional semi-micro LC system（2.5 µL, cell length 5 mm） Conventional semi-micro LC system（2.5 µL, cell length 5 mm）
Flow rate : 0.8 mL/min Flow rate : 0.36 mL/min
mAU 1 mAU
Injection : 2 µL Injection : 1 µL
2
80 3 80 1 3 N(1) = 3700
N(1) = 6300 2
60 N(2) = 6300 60 N(2) = 4100
4
N(3) = 7500 4 N(3) = 6100
40 40
N(4) = 7800 N(4) = 6900
20 Rs(1, 2) = 2.21 20 Rs(1, 2) = 1.61
0 0 (N070608E)
(N070606B)
0 1 2 3 min 0 1 2 3 min
Conventional LC system（8 µL, cell length 10 mm） Conventional LC system（8 µL, cell length 10 mm）
Flow rate : 0.8 mL/min Flow rate : 0.36 mL/min
mAU mAU Injection : 1 µL
Injection : 2 µL
150 1 3 N(1) = 3500 150 N(1) = 1400

2
N(2) = 3800 3 N(2) = 1500
100 12 N(3) = 3600
100 4 N(3) = 6100 4
N(4) = 6800 N(4) = 4500
50 50
Rs(1, 2) = 1.61 Rs(1, 2) = 0.90
0 0 (N070608B)
(N070606E)
0 1 2 3 min 0 1 2 3 min
Cephalosporin antibiotics
YMC-UltraHT series can be used for fast analysis with conventional 1. Cephalexin Column ：YMC-UltraHT Pro C18
LC system, as its pressure is lower than commercial sub-2 µm columns. 2. Cefaclor Eluent ：acetonitrile/20 mM KH2PO4
Considering the extra column band broadening, the column of 3.0 mml.D. is 3. Cephaloglycin 　(10/90)
4. Cephaloridine
more applicable for conventional LC system than the column of 2.0 mml.D.. Temperature ：37 ℃
25
第01章.indd 25 11.1.28 10:39:45 AM

Analytical columns
YMC-UltraHT Pro C18

● Superior column performance at higher flow rate and higher pressure ■Pore size：120 Å
● Excellent resolution with back pressure less than 60% of commercial sub-2 µm ■Carbon content：16％
● Same selectivity and superior peak shapes as 3 µm and 5 µm Pro C18 ■Usable pH range：2.0 〜 8.0
● Simple method transfer from conventional HPLC
First choice ODS column for Ultra-fast LC

YMC-UltraHT Pro C18 is a C18 bonded-phase based makes YMC-UltraHT Pro C18 highly appropriate for
on highly efficient 2 µm spherical silica particles. YMC- analysis of basic compounds. The physical properties and
UltraHT Pro C18 is designed for high-speed and high separation characteristics are strictly controlled, so it is
throughput analysis, advanced endcapping technology highly suitable as the first choice column for ultra fast LC.
Excellent peak shapes

YMC-UltraHT C18
50×2.0 mmI.D 2 µm sub-2 µm ODS sub-2 µm ODS
mV mV mV
60 1 60 1 60
N(5) 106,000/m 2
2 3 N(5) 89,000/m 1 N(5) 78,000/m
3 Tf(5) 1.07 2
40 40 Tf(5) 3.99 40 3 Tf(5) 2.35
4 30 MPa
4 49 MPa 4 37 MPa
20 5 20 20 5
5
0 0 0
（ ODS
0.0 0.4 0.8 1.2 1.6 2.0 0.0 0.4 0.8 1.2 1.6 2.0 0.0 0.4 0.8 1.2 1.6 2.0
[min] [min] [min]
）
Alkaloids Column
Eluent
: 50×2.0 or 2.1 mmI.D.
:2 0 mM CH3COOH-CH3COONH4 (pH 4.9)/
1. Scopolamine
YMC-UltraHT Pro C18 exhibits greater peak shapes of acetonitrile (80/20)
2. Atropine
alkaloids, which are highly basic compounds compared to Flow rate : 0.6 mL/min
3. Cinchonine
commercial silica based sub-2 µm columns. 4. Quinine
5. Dihydroquinine
Peptide mapping
mV
YMC-Pack C18 ＊ 0%B (0-5 min)
150×2.0 mmI.D. 5 µm 0-100%B (5-40 min)
100%B (40-45 min)
80
40
0
(06082204.D) Analysis of a tryptic digest of cytochrome C is
0 15 30 45 min
shown below. A 50mm length UltraHT Pro C18
same flow rate, column length 1/3x, gradient time 1/3x
column of 2 µm particle can be shorten the
mV
＊ 0%B (0-1.65 min) analysis time by one-thirds compared to a 150
YMC-UltraHT C18 0-100%B (1.65-13.35 min)
100%B (13.35-15 min) mm column packed with 5 µm Pro C18.
50×2.0 mmI.D. 2 µm
200
Eluent ：A) acetonitrile/water/TFA (10/90/0.1)

100 　B) acetonitrile/water/TFA (35/65/0.1)
*undigested
Cytochrome c
0 （N060811I）
0 5 10 15 min
Sample ：tryptic degest of Cytochrome c
Analytical columns
Particle size Pore size Column size
Product number
（µm）（Å） inner diameter×length（mm）
S-2 120 2.0× 30 AS12S02-0302WT
2.0× 50 AS12S02-0502WT
2.0× 75 AS12S02-L502WT
2.0×100 AS12S02-1002WT
3.0× 50 AS12S02-0503WT
3.0× 75 AS12S02-L503WT
3.0×100 AS12S02-1003WT
26
第01章.indd 26 11.1.28 10:40:11 AM

Analytical columns
YMC-UltraHT Hydrosphere C18

● Can be used with 100% aqueous as mobile phase ■Pore size：120 Å
● Superior column performance at higher flow rate and higher pressure ■Carbon content：12％
● Reduced analysis time while maintaining excellent resolution ■Usable pH range：2.0 〜 8.0
The best column for hydrophilic compounds

YMC-UltraHT Hydrosphere C18 is a C18 bonded phase It is designed to maintain hydrohilicity on the packing
based on highly efficient 2 µm spherical silica particles. surface for superior separation of hydrophilic compounds.

1
Ultrafast analyses of soy isoflavones

Hydrosphere C18 50×4.6 mmI.D. 3 µm
Rs(10,11)=2.38
Examples illustrated shows the
3 9 10
12
12.5-30% B (0-10 min)
optimization process of isoflavones
1
2
6
11
1.5 mL/min separation by 2 µm YMC-UltraHT
7 8
4
5 3 µL injection Hydrosphere C18. It has the same
selectivity as 3 µm Hydrosphere C18,
so there is no need to change the
(N060509E)
condition at downsizing. Maintaining
resolution, the analysis time can
（ ODS
0 2 4 6 8 10 min be reduced by using 2 µm packing
material.
粒子サイズ、カラム内径をダウンサイジング
）
downsizing the particle size and column inner diameter
YMC-UltraHT Hydrosphere C18 2μm 50×2.0 mmI.D.
YMC-UltraHT Hydrosphere C18 50×2.0 mmI.D. 2 µm
Rs(7,8)=2.46 Rs(10,11)=2.91
1 3 Rs(10,11)=2.91
1 2 3 9 12
2 9 10
10 11
12 12.5-30% B (0-10 min)
6 7 11 0.3 mL/min
5
4 5 6 7 8 12.5-30% ACN (0-10 min)
4 8 0.6 µL injection
0.3 mL/min
MPa
(N070130I)
(N070130I) Soy isoflavones
0
0
2
2
4
4
6
6
8
8 10
10 min
min
1. Daidzin
2. Glycitin
流速3倍、グラジエント条件最適化 3. Genistin
triple flow rate and optimizing the gradient condition 4. 6"-O-Malonyldaidzin
5. 6"-O-Malonylglycitin
YMC-UltraHT Hydrosphere C18 2μm 50×2.0 mmI.D. 6. 6"-O-Acetyldaidzin
YMC-UltraHT Hydrosphere C18 50×2.0 mmI.D. 2 µm 7. 6"-O-Acetylglycitin
Rs(10,11)=2.64 8. 6"-O-Malonylgenistin
Rs(10,11)=2.64
Rs(7,8)=1.75 12 9. Daidzein
1 3 9
3
10 12 12.5-30% B (0-3.3 min) 10. Glycitein
1 2 9 10 11
0.9 mL/minACN (0-3.3 min)
12.5-30% 11. 6"-O-Acetylgenistin
2 6 7 8 11
0.6 µL injection 12. Genistein
4
5
6 7 8
0.9 mL/min
5
4
MPa
(N070130L)
(N070130L)
0 1 2 3 3.3 min Eluent ：A) water/acetic acid (100/3)
0 1 2 3 3.3 min 　B) acetonitrile/acetic acid (100/3)
Analytical columns
Particle size Pore size Column size
Product number
（µm）（Å） inner diameter×length（mm）
S-2 120 2.0× 30 HS12S02-0302WT
2.0× 50 HS12S02-0502WT
2.0× 75 HS12S02-L502WT
2.0×100 HS12S02-1002WT
3.0× 50 HS12S02-0503WT
3.0× 75 HS12S02-L503WT
3.0×100 HS12S02-1003WT
27
第01章.indd 27 11.1.28 10:40:30 AM

High performance reversed-phase column
Features of Pro series ODS columns

Pro series high performance reversed-phase columns We provide products with excellent performance and
feature advanced endcapping technology and quality stable quality all over the world to fulfill the needs of the
assurance which is ready for validation. The Pro series pharmaceutical industry, in which production, as well as
includes three kinds of ODS, each with unique separation research and development, are increasingly globalized.
characteristics.
1
Providing products with stable quality all over the world
Feature
● Silica
gel base, low in metal impurities
● Processed
with advanced endcapping
technology
● Superior
separation of basic compounds
（ ODS
●U
seful for LC/MS
）
●E
xcellent durability
●E
xcellent reproducibility
● Two
types of test reports are included
with each column
Standard ODS column Low carbon ODS column, High carbon ODS column,
with high versatility useful for separation of useful for separation of highly
hydrophilic compounds hydrophobic compounds
YMC-Pack Pro C18 Hydrosphere C18 YMC-Pack Pro C18 RS
YMC-Pack C18 is a high Hydrosphere C18 is an ODS column YMC-Pack C18 RS is a high
performance monomeric type ODS designed to maintain maximum carbon "polymeric type" ODS bonding,
column providing standard hydrophilicity without activating the characterized by high resolution and
hydrophobicity, high resolution, high silica surface. It can be used with high durability. It is applicable to a
durability and excellent reproducibility. 100% water eluent, a problematic wide range of compounds. The
It is highly versatile and suitable as the eluent for conventional ODS columns. separation of compounds with relatively
first choice column in almost all fields, Hydrosphere C18 is useful for high hydrophobicity and those that differ
including pharmaceutical products, separating hydrophilic compounds. only slightly in hydrophobicity, such as
agricultural chemicals, foods, and structural isomers, is outstanding. It
natural products. also has excellent resistance to acid
and alkali, making it useful under
demanding separation conditions.
Types of Pro series ODS

Pro C18 Hydrosphere C18 Pro C18 RS
Particle Size (µm) 2 , 3 , 5 , 10 2,3,5 3,5
Pore Size (Å) 120 120 80
Specific Surface area (m /g) 2
330 330 510
Carbon Content 16% 12% 22%
Bonding type monomeric monomeric polymeric
Available pH Range 2-8 2-8 1 - 10
Characteristics Standard type Separation for hydrophilic compounds High durability
28
第01章.indd 28 11.1.28 10:40:32 AM

Three kinds of ODS, each with different separation characteristics
The three kinds of ODS columns in the Pro series

each have unique carbon content and separation
characteristics, including hydrophobicity, plane
recognition ability, and hydrogen-bonding capacity.
Selection according to the analyte and mobile phase
of Pro C18 with standard hydrophobicity, Pro C18 RS
with high hydrophobicity, or Hydrosphere C18 with low
hydrophobicity, facilitates method development.
Hydrophobicity and plane recognition ability of Pro series ODS

YMC-Pack C18 Hydrosphere C18 YMC-Pack C18 RS 1
1 3
1 1
3
5
2
2 5 3
4 2
4 5
4
（ ODS
0 5 10 15 20 min 0 5 10 15 20 min 0 5 10 15 20 25 min
）
J020805B J020805D J020805E
1. Uracil Column size ：150×4.6 mmI.D.

2. n-Butylbenzene Eluent ：methanol/water (80/20)
3. o-Terphenyl Flow rate ：1.0 mL/min
4. n-Amylbenzene Temperature ：37 ℃
5. Triphenylene Detection ：UV at 254 nm
Comparison of hydrophobicity and hydrogen-bonding capacity of various columns
0.180
Hydrogen-bonding capacity α (caffeine/benzene)
Hydrophobicity: low
0.160
Hydrogen-bonding capacity: high
Develosil C30-UG
0.140 Hydrosphere C18
Atlantis T3
CAPCELL PAK C18 AQ Hydrophobicity: intermediate
Hydrogen-bonding capacity: middle
0.120
CAPCELL PAK C18 MGIII
Gemini C18 Hydrophobicity: high
Pro C18 Hydrogen-bonding capacity: low
0.100 Unison UK-C18 Inertsil ODS-4
Hypersil GOLD CAPCELL PAK C18 MGII
Gemini NX-C18
SunFire Inertsil ODS-3 Develosil ODS-SR
XBridge C18
XTerra MS C18
Eternity-C18 Mightysil RP-18 C18
L-column ODS
0.080
Cadenza CD-C18
TSKgel ODS-100S L-column2 ODS
Pro C18 RS
0.060
Eluent : MeOH/W (80/20) [amylbenzene], MeOH/W (30/70) [caffeine, benzene]

0.040
2.00 4.00 6.00 8.00 10.00 12.00 14.00
(S090805A)
Hydrophobicity k’ (amylbenzene)
29
第01章.indd 29 11.1.28 10:40:35 AM

High performance reversed-phase column
Features of Pro series ODS columns
Comparison of separation behavior on the three kinds of ODS columns in the Pro series
Parabenzoates
C18 Hydrosphere C18 C18 RS
3 types of ODS are available to
separate the para-benzoates
shown in the left, but the best
separation between peaks 5 and
6 is achieved on Pro C18 RS.
1 1 1
1
1.
COOCH3
OH
2 2
2 Methyl p-hydroxybenzoate
2.
OH
COOCH2CH3
34 34 Ethyl p-hydroxybenzoate
3 4
3. CH3
OH
COOCH
（ ODS
CH3
5
6 56 Isopropyl p-hydroxybenzoate
5 6
4.
）
OH
COO(CH2)2CH3
Propyl p-hydroxybenzoate
5. OH
CH3
COOCH2CH
0 10 20 min 0 10 20 min 0 10 20 30 min CH3
（J020827D）（J020827C）（J020827E）
Isobutyl p-hydroxybenzoate
6. OH
COO(CH2)3CH3
Column ：150×4.6 mmI.D.
Eluent ：methanol/water (55/45) Butyl p-hydroxybenzoate
Flavonoid glycosides
2 Hydrogen-bonding
interactions significantly
1
3 contribute to the retention
C18 4 of hydrophilic compounds,
including glycosides.
Therefore, the retention is
longer in the Hydrosphere
J020828A HO C18, which has strong
2
HO hydrogen-bonding capacity,
1 O OH than in Pro C18 RS, which
3
OR has highly hydrophobic
Hydrosphere C18 O packing materials. To
4 OH
separate such hydrophilic
1. Rutin R = rutinose
compounds, the Hydrosphere
2. Isoquercitrin R = glucose
3. Quercitrin R = rhamnose C18 is suitable for
J020828F
2 4. Quercetin R =H precise separation of
multicomponent samples,
1 3 while Pro C18 RS and
4 Pro C18 are suitable for
C18 RS rapid separation of simple
components.
J020828C
0 5 10 15 min Eluent ：acetonitrile/10 mM H3PO4 (25/75)
30
第01章.indd 30 11.1.28 10:40:39 AM

Terphenyl isomers
C18 Hydrosphere C18 C18 RS Brand C1
Since Pro C18 RS has
2
excellent hydrophobic
selectivity and shape
selectivity, it works
well in the separation
2 2,3 of structural isomers

that are not easily 1
separated by other
2 ODS.
1.
1 3
1
o-Terphenyl
3
1
3 1 2.
（ ODS
m-Terphenyl
）
3.
0 10 20 min 0 10 20 min 0 10 20 min 0 10 20 min

J020719J J020722A J020719E J020719K
p-Terphenyl

Eluent ：methanol/water
　(85/15)
Saikosaponins
1 Peaks 2 and 3, which are not easily separated by other

ODS, can be completely separated by Hydrosphere C18. It
C18 3 is thought that hydrogen-bonding interaction of hydrophilic
2
4
functional groups in sugar chain and terpene skeleton as
well as hydrophobic interaction has great contribution to the
separation of saikosaponins.
Hydrosphere C18 is the most appropriate in the separation of
F030222B
1
this kind of class of compound.
Hydrosphere C18 23
4 1. 3.
O
H CH2OH
F030222A OH OH
1 O O
CH2OH
2,3 glucose 6 glucose
4
fucose glucose
3
rhamnose
C18 RS
Saikosaponin c Saikosaponin b2
4
2. 4.
O O
F030222C
H H
1
OH OH
2,3 O O
CH2OH CH2OH
Brand B2 fucose glucose fucose glucose
3 3
4
Saikosaponin a Saikosaponin d
F030224E
0 10 20 30 40 min Flow rate ：1.0 mL/min
31
第01章.indd 31 11.1.28 10:40:44 AM

Analytical columns
YMC-Pack Pro C18

●S
uperior separation of basic ● Excellent reproducibility ■Pore size：120 Å
compounds ● Utilizes highly pure silica gel base ■Carbon content：16％
●P
rocessed with advanced ●T wo types of test reports are included ■Usable pH range：2.0 〜 8.0
endcapping technology with each column
Perfectly endcapped ODS

YMC-Pack Pro C18 includes more advanced endcapping technology for Two test reports are included with each product; a Column Test Report,
strictly controlled processing of residual silanol groups that are likely indicating the column performance, and a Packing Material Test
to affect quality. Pro C18 is highly appropriate for basic compounds, Report, detailing strictly controlled physical properties and separation
including pharmaceutical products and agricultural chemicals. characteristics of the packing material.
Useful for separation of basic compounds

Tricyclic antidepressants
23 1. 2.
1 ・ HCI Advanced endcapping technology
4
N results in good peak shapes even
CH2CH2CH2NHCH3
for basic compounds that often
Desipramine hydrochloride Acenaphthene(I.S.) elute with poor tailing shapes on
competitive columns.
3. 4.
（ ODS
・ HCI ・ HCI
N CH3
CH3 CHCH2CH2N
CH2CH2CH2N
）
CH3
CH3 Column ：YMC-Pack Pro C18 (5 µm, 120 Å)
Imipramine hydrochloride Amitriptyline hydrochloride 　150×4.6 mmI.D.
Eluent ：20 mM KH2PO4-K2HPO4 (pH6.9)/
　methanol (25/75)
0 10 20 min Detection ：UV at 254 nm
（A961015G）
Basic peptides
1. Bradykinin
C18 ODS-A 2. Dynorphin A (Human,1-13) YMC-Pack Pro C18 is processed
3. Eledoisin related peptide
4. Neurotensin
using advanced endcapping
5. Bombesin technology and produces a
4
1
4 6. Substance P better chromatogram than
6
5 1 5 that obtained using ODS-A for
2 6
separating basic peptides.
3 2
Similar chromatograms have
3
been obtained with YMC-
Pack Pro C18 and ODS-A for
separation of neutral peptides.
Column ：YMC-Pack Pro C18 and ODS-A (5 µm, 120 Å)

0 5 10 15 20 min 0 5 10 15 20 min 　75×4.6 mmI.D.
（A990123B）（A990121A） Eluent ：A) water/TFA (100/0.1)
　B) acetonitrile/TFA (100/0.1)
　20-40％ B (0-20 min,linear)
Neutral peptides Flow rate ：1.0 mL/min
C18 ODS-A Detection ：UV at 220 nm
2
5 2
3 4 5
6 3
4 6
1
1
1. Oxytocin
2. Met-Enkephalin
3. Leu-Enkephalin
4. Angiotensin l
0 5 10 15 20 min 0 5 10 15 20 min 5. α-Mating factor
（A990121B）（A990119B） 6. Insulin
32
第01章.indd 32 11.1.28 10:40:48 AM

Excellent lot-to-lot reproducibility
batch-to-batch
Test A Test B
A three-lot comparison is shown on the left.
1. Uracil 1. Caffeine Excellent reproducibility is achieved in
2. 4-n-Butoxybenzoic acid 2. Pyridine separating hydrophilic, basic and acidic
3. Ethylbenzene 3. Phenol
compounds, as well as hydrophobic
4. n-Propylbenzene
compounds.

1
Lot C Lot C
Lot B Lot B
Lot A Lot A
（ ODS
0 5 10 15 min 0 4 8 min
）
column-to-column
150×4.6 mml.D. theoretical plate number
24000 Advanced packing technology results in high
column efficiency. All packed columns are
21000
inspected and controlled according to the
18000
strictest quality control criteria for column
performance ever, including theoretical plate
15000 number testing.
12000
9000
6000
3000
20 40 60 80 100
Column No.
Two types of test reports

〈Packing Material Test Report〉〈Column Test Report〉
Each YMC-Pack Pro C18

includes both, a Column Test
Report and a Packing Material
Test Report, to certify lot-
to-lot and column-to-column
reproducibility.
This report describes basic physical properties of silica The Column Test Report shows the theoretical plate number
gel, including particle size, pore size and metal content, and asymmetry factor to indicate column performance. For
carbon loading after modification, and the specifications convenience in deterioration evaluation, this report includes all
and results of separation characteristics tests. analytical conditions including sample concentration as well.
33
第01章.indd 33 11.1.28 10:40:51 AM

Analytical columns
YMC-Pack Pro C18
Application
3 4 5 3 4 5
4, 5
1 3
C18 Brand I4
Brand L1
1
1
2
2
2
1
6 6
F040119B F030130C 6
F040130E
0 5 10 15 20 min 0 5 10 15 20 min
0 5 10 15 20 min
nasal drops (OTC)

1 2 3 4 N Column ：150×4.6 mmI.D.
CH3 O
Eluent ：A) 20 mM KH2PO4-H3PO4 (pH 2.5)
CH3
CHCOOH NHCCH2 N(CH2 CH3 )2 CHCH2CH2N
H CH3
CHCOOH CH2
　B) methanol
N
CH3
　20-90% B (0-15 min), 90% B (15-20 min)

N
Cl
Maleic acid Lidocaine Naphazoline Chlorpheniramine
5 6 CH3 CH3 CH3 Temperature ：37 ℃
+
Cl-
OH
COOCH3 CH3 C CH2 C (OCH2CH2)2 N CH2
CH3 CH3 CH3
Methyl p-hydroxybenzoate Benzethonium chloride
（ ODS
Application
）
（N050620A）
catechins
1. (-)-Epigallocatechin
2. (+)-Catechin
1
3 3. (-)-Epicatechin
4
4. (-)-Epigallocatechin gallate
5. (-)-Epicatechin gallate
2
5 Column : YMC-Pack Pro C18 (5 µm, 120 Å)

150×4.6 mmI.D.
Eluent : acetonitrile/ethyl acetate/water/TFA
(9/3/88/0.1)
Flow rate : 1.0 mL/min
0 10 20 min
Application （A970526C）
antioxidants
5
1. n-Propyl gallate(PG)
2. 2,4,5-Trihydroxybutyrophenone (THBP)
4
3. tert-Butylhydroquinone(TBHQ)
4. Nordihydroguaiaretic acid (NDGA)
6 5. 4-Hydroxymethyl-2,6-di-tert-butylphenol (HMBP)
7 6. n-Octyl gallate(OG)
1 7. n-Dodecyl gallate(DG)
2
3 Column ：YMC-Pack Pro C18 (5 µm, 120 Å)

　75×4.6 mm I.D.
Eluent ：A) acetonitrile/methanol/5% acetic acid (20/20/60)
　B) acetonitrile/methanol/5% acetic acid (45/45/10)
　0-100% B (0-5 min), 100% B (5-15 min)
0 5 10 15 min
34
第01章.indd 34 11.1.28 10:40:55 AM


S-3 120 1.0× 35 AS12S03-H501WT S-5 120 10×150 AS12S05-1510WT
1.0× 50 AS12S03-0501WT 10×250 AS12S05-2510WT

1.0×100 AS12S03-1001WT 20×100 AS12S05-1020WT 1
1.5× 35 AS12S03-H5P5WT 20×150 AS12S05-1520WT
1.5× 50 AS12S03-05P5WT 20×250 AS12S05-2520WT
1.5×100 AS12S03-10P5WT S-10 120 20×100 AS12S11-1020WT
2.0× 35 AS12S03-H502WT 20×150 AS12S11-1520WT
2.0× 50 AS12S03-0502WT 20×250 AS12S11-2520WT
2.0× 75 AS12S03-L502WT See P142 for preparative columns other than those listed above.
2.0×100 AS12S03-1002WT
2.0×150 AS12S03-1502WT
3.0× 50 AS12S03-0503WT
3.0×100 AS12S03-1003WT
Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
3.0×150 AS12S03-1503WT S-3 120 1.0×10 AS12S03-0101CC
4.6× 35 AS12S03-H546WT 1.5×10 AS12S03-01P5CC
（ ODS
4.6× 50 AS12S03-0546WT 2.0×10 AS12S03-0102CC
4.6× 75 AS12S03-L546WT 4.0×23 AS12S03-G304CC
4.6×100 AS12S03-1046WT S-5 120 1.0×10 AS12S05-0101CC
）
4.6×150 AS12S03-1546WT 1.5×10 AS12S05-01P5CC
6.0× 50 AS12S03-0506WT 2.0×10 AS12S05-0102CC
6.0× 75 AS12S03-L506WT 4.0×23 AS12S05-G304CC
6.0×100 AS12S03-1006WT A guard cartridge holder will need to be purchased separately before using this
product for the first time.
S-5 120 1.0×150 AS12S05-1501WT
1.0×250 AS12S05-2501WT
1.5×150 AS12S05-15P5WT
1.5×250 AS12S05-25P5WT Cartridge holder
2.0× 35 AS12S05-H502WT
2.0× 50 AS12S05-0502WT
Semi-micro guard cartridge holder
XPCHSMW
2.0× 75 AS12S05-L502WT (inner diameter: 1.0, 1.5, or 2.0 mm)
2.0×100 AS12S05-1002WT Cartridge holder
2.0×150 XPCHW
AS12S05-1502WT (inner diameter: 4.0 mm)
2.0×250 AS12S05-2502WT
3.0× 50 AS12S05-0503WT
3.0× 75 AS12S05-L503WT
3.0×100 AS12S05-1003WT YMC guard cartridge pack
3.0×150 AS12S05-1503WT
3.0×250 AS12S05-2503WT S-3 120 4.0×23 AS12S03-G304CCPK
4.6× 50 AS12S05-0546WT S-5 120 4.0×23 AS12S05-G304CCPK
4.6× 75 AS12S05-L546WT A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge
4.6×100 AS12S05-1046WT column, and a column coupler (produced by PEEK).
4.6×150 AS12S05-1546WT
4.6×250 AS12S05-2546WT
6.0×150 AS12S05-1506WT
6.0×250 AS12S05-2506WT Guard columns
S-10 4.6×150 AS12S11-1546WT S-5 120 10×30 AS12S05-0310WTG
120
4.6×250 AS12S11-2546WT 20×50 AS12S05-0520WTG
S-10 120 20×50 AS12S11-0520WTG
When columns packed with material from different lots are needed for validation
purposes, please contact us.
35
第01章.indd 35 11.1.28 10:40:56 AM

Analytical columns
Hydrosphere C18
● Strong retention of hydrophilic compounds ● Utilizes highly pure silica gel base ■Pore size：120 Å
● Can be used with 100% water mobile phase ●T wo types of test reports are included with ■Carbon content：12％
● Superior separation of basic compounds each column ■Usable pH range：2.0 〜 8.0
● Excellent reproducibility
Hydrophilic ODS
Hydrosphere C18 is designed to maintain adequate hydrophilicity on with 100% water eluent, a problematic eluent for conventional ODS
the packing surface for superior separation of hydrophilic compounds. columns. Hydrosphere C18 is useful for separating a wide range of
Hydrophilic compounds are retained with much greater affinity than compounds, including nucleic acids and their derivatives, organic
with conventional ODS columns, permitting the product to be used acids, saccharides, glycosides and peptides.
Appropriate for separation of hydrophilic compounds

Separation of organic acids
Hydrosphere C18 conventional ODS column（BrandⅠ）
High hydrophilicity of the
2
2 Hydrosphere C18 packing
1
3,4 material surface enables
longer retention and superior
1
5
separation of citric acid and
5
4 fumaric acid as compared to
（ ODS
3
a conventional ODS column
(Brand Ⅰ).
）
Column ：150×4.6 mm I.D.

1. Lactic acid Eluent ：20 mM NaH2PO4-H3PO4 (pH 2.8)
2. Acetic acid Flow rate ：1.0 mL/min
3. Citric acid Temperature ：30 ℃
0 5 10 min 0 5 10 min 4. Fumaric acid Detection ：UV at 210 nm
A000310E A000615A 5. Succinic acid
Reproducibility of retention time when used with 100% water mobile phase
Hydrosphere C18 conventional ODS column（Brand Ⅱ） conventional ODS column（Brand Ⅲ）
3 When conventional ODS columns are
Before standing
3 used with 100% water mobile phase,

3 2
2 2 the apparent hydrophobicity decreases
1 1
1 due to repulsive interactions between
5※ water and hydrophobic groups on
5※ 4 5※
4 4 the surface of the packing materials,
reducing retention of compounds.
In the experiment illustrated here,
columns filled with 100% water mobile
0 10 20 min 0 10 20 min
S0515A
0
S0518H
10 20 min
S0518F
phase were left to stand overnight.
Retention times of nucleic acid bases
were analyzed before and after
After standing
standing and compared. The retention

times of adenine after standing
※
decreased to 89% of the original
※ 71％ value for Brand II and to 71% of the
※ 98％ 89％
original Brand III value. By contrast,
Hydrosphere C18 retained 98% of
its original retention for adenine.
0 10 20 min 0 10 20 min
Hydrosphere C18 is designed to
0 10 20 min
S0517A S0519A S0519C maintain adequate hydrophilicity
After completion of analysis, the flow was stopped and the columns were left to stand overnight (ca. 15 hours). on the packing surface so that
hydration can be achieved, and when
Hydrosphere C18 is used with mobile
Column ：150×4.6 mm I.D. phase containing no organic solvent,
1. Cytosine Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9) the retention time is not significantly
2. Uracil Flow rate ：1.0 mL/min
3. Guanine shortened and highly reproducible
4. Thymine Detection ：UV at 254 nm chromatograms can be obtained.
5. Adenine
36
第01章.indd 36 11.1.28 10:41:01 AM

Differences in separation characteristics from conventional ODS columns
Hydrosphere C18
The chromatograms shown were obtained in tests
conducted to compare separation characteristics using
1
a mixed sample containing compounds with different
2
characteristics.
The packing material surface of Hydrosphere C18 has
4
higher hydrophilicity and lower C18 ligand density than

3 5 6 those of Pro C18. This results in shorter retention of 1
hydrophobic compounds, including n-propylbenzene
and n-butylbenzene, and planar compounds like
acenaphthene. By contrast, retention of testosterone,
0 10 20 30 40 min
a compound with large hydrogen-bonding properties, is
S000309A
longer on Hydrosphere C18 and on Brand IV, which also

has a highly hydrophilic packing surface.
Amitriptyline, a basic compound, caused tailing peaks
C18 in Brand IV and Brand V. By contrast, Hydrosphere C18
and Pro C18 produce good peak shapes for such basic
2 compounds. Although the retention times of amitriptyline
1
on Hydrosphere C18 and Pro 18 are similar, there is a
big difference between the two columns with regard to
（ ODS
resolution of n-butylbenzene and amitriptyline. This
difference is considered to be due to the hydrophilicity of
4
3 5 6 the Hydrosphere C18 surface.
）
These results demonstrate that Hydrosphere C18, having
different separation characteristics from conventional
ODS columns, enables separation of compounds that
0 10 20 30 40 min
S990607B cannot be separated using other ODS columns.
Brand Ⅳ
1
2
4
3 5
0 10 20 30 40 50 60 min
F000614B
Brand Ⅴ
2
1
3 4 5
0 10 20 30 40 50 60 70 80 90 min
F000614C
1. Toluene Column ：150×4.6 mm I.D.

2. Testosterone Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (35/65)
3. n-Propylbenzene Flow rate ：1.0 mL/min
4. Acenaphthene Temperature ：37 ℃
5. n-Butylbenzene Detection ：UV at 254 nm
6. Amitriptyline hydrochloride
37
第01章.indd 37 11.1.28 10:41:04 AM

Analytical columns
Hydrosphere C18
Short analysis time

conventional ODS column Hydrosphere C18
3 Hydrosphere C18 can reduce the time necessary
3 for analyzing compounds with high hydrophobicity
by about 20% as compared with conventional ODS
2 4 6 columns.
2 4 5
5 6
Antioxidants
1 1
1
1. Nordihydroguaiaretic acid (NDGA)

2. Butyl hydroxyanisol (BHA)
3. 4-Hydroxy-2,6-di-tert-butylphenol (HMBP)
4. n-Octyl gallate (OG)
5. Dibutyl hydroxytoluene (BHT)
6. n-Dodesyl gallate (DG)
Column ：50×4.6 mm I.D. (3 µm, 120 Å)

Eluent ：acetonitrile/methanol/5% acetic acid (35/35/30)
0 5 10 15 min 0 5 10 15 min
J010216B J010215B Detection ：UV at 280 nm
（ ODS
Application （F000707B）
Nicotinic acid and derivatives

）
1 2 4 1. Nicotinamide adenine dinucleotide phosphate oxidized form (NADP)

2. Nicotinic acid
3. Nicotinamide adenine dinucleotide phosphate reduced form (NADPH)
4. Nicotinamide
5. β-Nicotinamide adenine dinucleotide (NAD)
5 6. β-Nicotinamide adenine dinucleotide reduced form (NADH)
Column ：Hydrosphere C18 (5 µm, 120 Å)

3 　150×4.6 mm I.D.
Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (99/1)
6 Temperature ：37 ℃
Detection ：UV at 260 nm　
0 5 10 15 20 25 min
Application （A000922A）
Nucleotides
5 1. 5’-UTP 7. 5’-ATP
1 2. 5’-UDP 8. 5’-IMP
3. 5’-CMP 9. 5’-ADP
4. 5’-UMP 10. 5’-TMP
7 5. 5’-dCMP 11. 5’-AMP
6. 5’-GMP
2
4
8 10
Column ：Hydrosphere C18 (5 µm, 120 Å)
3 6 11 　150×4.6 mm I.D.
9 Eluent ：100 mM KH2PO4-K2HPO4 (pH 5.5)
0 5 10 15 20 min
38
第01章.indd 38 11.1.28 10:41:07 AM


S-3 120 1.0× 35 HS12S03-H501WT S-5 120 10×150 HS12S05-1510WT
1.0× 50 HS12S03-0501WT 10×250 HS12S05-2510WT

1.0×100 HS12S03-1001WT 20×100 HS12S05-1020WT 1
1.5× 35 HS12S03-H5P5WT 20×150 HS12S05-1520WT
1.5× 50 HS12S03-05P5WT 20×250 HS12S05-2520WT
1.5×100 HS12S03-10P5WT
2.0× 35 HS12S03-H502WT
2.0× 50 HS12S03-0502WT
2.0× 75 HS12S03-L502WT Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
2.0×100 HS12S03-1002WT S-3 120 1.0×10 HS12S03-0101CC
2.0×150 HS12S03-1502WT 1.5×10 HS12S03-01P5CC
3.0× 50 HS12S03-0503WT 2.0×10 HS12S03-0102CC
3.0×100 HS12S03-1003WT 4.0×23 HS12S03-G304CC
3.0×150 HS12S03-1503WT S-5 120 1.0×10 HS12S05-0101CC
4.6× 35 HS12S03-H546WT 1.5×10 HS12S05-01P5CC
（ ODS
4.6× 50 HS12S03-0546WT 2.0×10 HS12S05-0102CC
4.6× 75 HS12S03-L546WT 4.0×23 HS12S05-G304CC
4.6×100 HS12S03-1046WT A guard cartridge holder will need to be purchased separately before using this
）
4.6×150 HS12S03-1546WT product for the first time.
6.0× 50 HS12S03-0506WT
6.0× 75 HS12S03-L506WT
6.0×100 HS12S03-1006WT Cartridge holder
S-5 120 1.0×150 HS12S05-1501WT
1.0×250 HS12S05-2501WT Semi-micro guard cartridge holder
XPCHSMW
1.5×150 HS12S05-15P5WT (inner diameter: 1.0, 1.5, or 2.0 mm)
1.5×250 HS12S05-25P5WT Cartridge holder
2.0× 35 HS12S05-H502WT XPCHW
2.0× 50 HS12S05-0502WT (inner diameter: 4.0 mm)
2.0× 75 HS12S05-L502WT
2.0×100 HS12S05-1002WT
2.0×150 HS12S05-1502WT
YMC guard cartridge pack
2.0×250 HS12S05-2502WT
3.0× 50 HS12S05-0503WT S-3 120 4.0×23 HS12S03-G304CCPK
3.0× 75 HS12S05-L503WT S-5 120 4.0×23 HS12S05-G304CCPK
3.0×100 HS12S05-1003WT
A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge
3.0×150 HS12S05-1503WT column, and a column coupler (produced by PEEK).
3.0×250 HS12S05-2503WT
4.6× 50 HS12S05-0546WT
4.6× 75 HS12S05-L546WT
4.6×100 HS12S05-1046WT Guard columns
4.6×150 HS12S05-1546WT S-5 120 10×30 HS12S05-0310WTG
4.6×250 HS12S05-2546WT 20×50 HS12S05-0520WTG
6.0×150 HS12S05-1506WT
6.0×250 HS12S05-2506WT
39
第01章.indd 39 11.1.28 10:41:08 AM

Analytical columns
YMC-Pack Pro C18 RS

●E xcellent acid resistance and alkali ● Excellent reproducibility ■Pore size：80 Å
resistance (pH 1 to 10) ● Utilizes highly pure silica gel base ■Carbon content：22％
● Superior separation of structural isomers ●T wo types of test reports are included ■Usable pH range：1.0 〜 10.0
● Superior separation of basic compounds with each column
Highly durable ODS

YMC-Pack Pro C18 RS is a polymeric type high carbon ODS tailing peaks. The separation selectivity for compounds
column characterized by high resolution and high durability. that differ only slightly in hydrophobicity is outstanding. It
It is applicable to a wide range of compounds, providing also has excellent resistance to acid and alkali, making it
good separation of basic compounds that easily cause useful under demanding separation conditions.
Excellent durability
pH 1 The C18 RS packing material hydrophobicity is hardly changed pH 10 Unlike conventional ODS columns, C18 RS
under the harsh conditions of pH1 and column temperature 70 ℃. can be used with an alkali mobile phase.
100 100
.
80 80
（ ODS
60 60
YMC-Pack Pro C18 RS
40 40
Acid-resistant column
）
( )
(manufactured by other company) Pro C18 RS

%
20 20
Monomeric ODS Monomeric ODS
%
0 0
0 20 40 60 80 100(hours) 0 200 400 600 800(hours)
Hours exposure to acetonitrile/water/TFA (10/90/1), Sequential analysis using 20 mM H3BO3-NaOH (pH 9.8)/methanol (50/50)
change in packing material hydrophobicity at 70 ℃.
A wide choice of mobile phases facilitates method development
150×4.6 mmI.D. 5 µm 50×4.6 mmI.D. 3 µm
pH 3.2 pH 6.8 pH 8.8 pH 8.8

3,4,5
Downsizing 12 3 4
1 2
2 12
4 3
3 4
5
5
1
5
0 5 10 15 20 min 0 5 10 15 20 min 0 5 10 15 20 min 0 5 10 min

F040716A F040714E F040714C F040714D
Pro C18 RS with excellent acid and alkali resistance can

be used over a wide pH range, and it is easy to optimize the
separating conditions. In addition, by downsizing from the
Column ：YMC-Pack Pro C18 RS column (particle 5 µm, length 150 mm) to a shorter column
1. Flurbiprofen
2. Methyl benzoate
Eluent ：20 mM acetate buffer/methanol (35/65) (particle 3 µm, length 50 mm), with high separation ability, it
Flow rate ：1.0 mL/min becomes possible to shorten the analysis time by one third
3. Lidocaine
4. Diltiazem
Detection ：UV at 220 nm without affecting the separation.
5. Verapamil
40
第01章.indd 40 11.1.28 10:41:12 AM

Separation of basic compounds
YMC-Pack C18 RS Acid-resistant column manufactured Amitriptyline is a basic compound that can
by other company（Brand D3） easily tail on some silica based columns. In
comparison with competing acid-resistant
columns, Pro C18 RS produces good peak
shapes without tailing.
Tf(4) 1.16 2
2
Tf(4) 2.87

3 1. Desipramine 1
1 4 2. Diphenyl (I.S.)
3
4 3. Imipramine
4. Amitriptyline
1
Eluent ：20 mM KH2PO4-K2HPO4 (pH 6.9)
/methanol (25/75)
0 10 20 min 0 10 20 min Flow rate ：1.0 mL/min
J011114B J020523D
Separation of structural isomers
（ ODS
Pro C18 RS with a high C18 density is excellent
Brand F1 C18 RS in hydrophobicity recognition ability and plane
）
(standard-type ODS 5 µm) 5 µm recognition ability, and can be applied to
separate structural isomers which are difficult
to separate with other ODS. In addition, by
using 3 µm column packing, resolution can be
1 1
increased, and it becomes possible to achieve
a baseline separation of linolenate isomers.
2 2
Rs=1.24
Rs=0.8
0 10 20 30 min 0 F040714 10 20 30 min

F040713D
Methyl linolenate isomers

1. Methyl linolenate
2. Methyl γ-linolenate

Eluent ：acetonitrile/THF/water (35/30/35)
C18 RS Temperature ：30 ℃
3 µm Detection ：UV at 210 nm
Rs=1.54
0 10 20 30 min
F040713A
41
第01章.indd 41 11.1.28 10:41:15 AM

Analytical columns
YMC-Pack Pro C18 RS
Separation of peptides by LC/MS
Pro C18 RS produces good peak shapes when

it is used with mobile phase containing low
100 530.95 TFA concentration, making it suitable for LC/
M+2 Bradykinin(MW: 1059.56)
MS.
%
531.47
1 M+1
1060.27
0
400 600 800 1000 1200(m/z)
1. [Arg8]-Vasopressin
2 3 5 2. Bradykinin
100 6
3. Angiotensin Ⅱ
4. Oxytocin
5. Angiotensin Ⅰ
6. Leu-Enkephalin
1
% 4
Column ：YMC-Pack Pro C18 RS (5 µm, 80 Å)
　150×2.0 mmI.D.
Eluent ：A) water/TFA (100/0.01)
　B) acetonitrile/TFA (100/0.01)
（ ODS
18 　10-35% B (0-15 min)

5.00 10.00 15.00 （Time）
20.00
）
Detection ：ESI positive-mode
Application
Chloroacetophenone isomers Chlorophenol isomers
1. COCH3 1. OH
2 Cl Cl
2
o-Chloroacetophenone o-Chlorophenol
2. COCH3 2. OH
3 Cl Cl
p-Chloroacetophenone p-Chlorophenol
3. COCH3 3 3. OH
1
Cl 1 Cl
m-Chloroacetophenone m-Chlorophenol
Column ：YMC-Pack Pro C18 RS (5 µm, 80 Å)

Column ：YMC-Pack Pro C18 RS (5 µm, 80 Å) 　150×4.6 mmI.D.
　150×4.6 mmI.D. Eluent ：a cetonitrile/water/acetic acid
Eluent ：acetonitrile/water (40/60) (30/70/1)
0 10 20 min
0 10 20 min Temperature ：30 ℃ J011113D Temperature ：37 ℃
J011026B
42
第01章.indd 42 11.1.28 10:41:19 AM


S-3 80 1.0× 35 RS08S03-H501WT S-5 80 10×150 RS08S05-1510WT
1.0× 50 RS08S03-0501WT 10×250 RS08S05-2510WT

1.0×100 RS08S03-1001WT 20×100 RS08S05-1020WT 1
1.5× 35 RS08S03-H5P5WT 20×150 RS08S05-1520WT
1.5× 50 RS08S03-05P5WT 20×250 RS08S05-2520WT
1.5×100 RS08S03-10P5WT
2.0× 35 RS08S03-H502WT
2.0× 50 RS08S03-0502WT
2.0× 75 RS08S03-L502WT Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
2.0×100 RS08S03-1002WT S-3 80 1.0×10 RS08S03-0101CC
2.0×150 RS08S03-1502WT 1.5×10 RS08S03-01P5CC
3.0× 50 RS08S03-0503WT 2.0×10 RS08S03-0102CC
3.0×100 RS08S03-1003WT 4.0×23 RS08S03-G304CC
3.0×150 RS08S03-1503WT S-5 80 1.0×10 RS08S05-0101CC
4.6× 35 RS08S03-H546WT 1.5×10 RS08S05-01P5CC
（ ODS
4.6× 50 RS08S03-0546WT 2.0×10 RS08S05-0102CC
4.6× 75 RS08S03-L546WT 4.0×23 RS08S05-G304CC
4.6×100 RS08S03-1046WT A guard cartridge holder will need to be purchased separately before using this
）
4.6×150 RS08S03-1546WT product for the first time.
6.0× 50 RS08S03-0506WT
6.0× 75 RS08S03-L506WT
6.0×100 RS08S03-1006WT Cartridge holder
S-5 80 1.0×150 RS08S05-1501WT
1.0×250 RS08S05-2501WT Semi-micro guard cartridge holder
XPCHSMW
1.5×150 RS08S05-15P5WT (inner diameter: 1.0, 1.5, or 2.0 mm)
1.5×250 RS08S05-25P5WT Cartridge holder
2.0× 35 RS08S05-H502WT XPCHW
2.0× 50 RS08S05-0502WT (inner diameter: 4.0 mm)
2.0× 75 RS08S05-L502WT
2.0×100 RS08S05-1002WT
2.0×150 RS08S05-1502WT
2.0×250 RS08S05-2502WT
3.0× 50 RS08S05-0503WT S-3 80 4.0×23 RS08S03-G304CCPK
3.0× 75 RS08S05-L503WT
S-5 80 4.0×23 RS08S05-G304CCPK
3.0×100 RS08S05-1003WT
3.0×150 RS08S05-1503WT A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge
column, and a column coupler (produced by PEEK).
3.0×250 RS08S05-2503WT
4.6× 50 RS08S05-0546WT
4.6× 75 RS08S05-L546WT
4.6×100 RS08S05-1046WT Guard columns
4.6×150 RS08S05-1546WT 10×30 RS08S05-0310WTG
4.6×250 RS08S05-2546WT
S-5 80
20×50 RS08S05-0520WTG
6.0×150 RS08S05-1506WT
6.0×250 RS08S05-2506WT
43
第01章.indd 43 11.1.28 10:41:20 AM

Analytical columns
YMC-Pack ODS-A
● Conventional ODS column ■Pore size：120 Å, 200 Å, 300 Å
● Currently in use worldwide ■Carbon content：17％ (120 Å), 12％ (200 Å), 7％ (300 Å)
■Usable pH range：2.0 〜 7.5
Standard ODS
YMC-Pack ODS-A has a highly endcapped surface order to ensure stable quality.
structure and appropriate hydrophobicity for separation This product is highly regarded as the standard YMC-
of a wide range of compounds. It is produced under strict Pack packing material in various countries around the
quality control with respect to 50 or more parameters in world.
Quality control system for excellent performance

10 The graph indicates lot-to-lot reproducibility with respect
to hydrophobic interaction for 10 lots, an essential column
8 performance characteristic. Extremely stable lot-to-lot
tR (Butyl benzoate), min
reproducibility of the hydrophobic surface is achieved by strict

6 control of variables.
In addition to measurement of the physical properties, a wide
range of compounds including hydrophobic, acidic, basic and
4
coordination compounds are analyzed under optimal conditions
（ ODS
to evaluate column performance.

2
）
0
1 2 3 4 5 6 7 8 9 10
Lot Number
Application （S930422D） Application （G910523Q）
Analgesics 1 Carotenes
2 O CH3
1.α-Carotene
H 3C N 2.β-Carotene
1 CH3COHN OH 2 N
2
O N N
Acetaminophen CH3
Caffeine
1
3 3 CONH2
4
CH2= CHCH2
H 3C
OC2H5 CHCHCONHCONH 2
H 3C
Ethenzamide
Apronalide
4
Column ：YMC-Pack ODS-A
Column ：YMC-Pack ODS-A
　(5 µm, 120 Å)
　(5 µm, 120 Å)
　150×4.6 mmI.D.
　150×4.6 mmI.D.
Eluent ：acetonitrile/methanol/THF
Eluent ：methanol/water (40/60)
　(50/45/5)
0 10 20 30 min 0 10 20 min Detection ：UV at 450 nm
Analytical columns
S-3 120 2.0× 35 AA12S03-H502WT S-3 120 4.6× 75 AA12S03-L546WT
2.0× 50 AA12S03-0502WT 4.6×100 AA12S03-1046WT
2.0× 75 AA12S03-L502WT 4.6×150 AA12S03-1546WT
2.0×100 AA12S03-1002WT 6.0×100 AA12S03-1006WT
2.0×150 AA12S03-1502WT 6.0×150 AA12S03-1506WT
3.0× 50 AA12S03-0503WT
3.0×100 AA12S03-1003WT
3.0×150 AA12S03-1503WT
44
第01章.indd 44 11.1.28 10:41:23 AM

S-5 120 1.0×150 AA12S05-1501WT S-10 120 10×150 AA12S11-1510WT
1.0×250 AA12S05-2501WT 10×250 AA12S11-2510WT
1.5×150 AA12S05-15P5WT 10×300 AA12S11-3010WT
1.5×250 AA12S05-25P5WT 20×150 AA12S11-1520WT
2.0× 75 AA12S05-L502WT 20×250 AA12S11-2520WT
2.0×150 AA12S05-1502WT 200 20×150 AA20S11-1520WT
2.0×250 AA12S05-2502WT 20×250 AA20S11-2520WT
3.0×150 AA12S05-1503WT 300 10×150 AA30S11-1510WT
3.0×250 AA12S05-2503WT 10×250 AA30S11-2510WT
4.6× 75 AA12S05-L546WT 10×300 AA30S11-3010WT
4.6×100 AA12S05-1046WT 20×150 AA30S11-1520WT
4.6×150 AA12S05-1546WT 20×250 AA30S11-2520WT

1
4.6×250 AA12S05-2546WT See P145 for preparative columns other than those listed above.
4.6×300 AA12S05-3046WT
6.0×100 AA12S05-1006WT Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
6.0×150 AA12S05-1506WT S-3 2.0×10 AA12S03-0102CC
6.0×250 AA12S05-2506WT 4.0×23 AA12S03-G304CC
6.0×300 AA12S05-3006WT S-5 120 1.0×10 AA12S05-0101CC
200 4.6×150 AA20S05-1546WT 1.5×10 AA12S05-01P5CC
4.6×250 AA20S05-2546WT 2.0×10 AA12S05-0102CC
300 1.0×150 AA30S05-1501WT 4.0×23 AA12S05-G304CC
1.0×250 AA30S05-2501WT 200 4.0×23 AA20S05-G304CC
1.5×150 AA30S05-15P5WT 300 1.0×10 AA30S05-0101CC
1.5×250 AA30S05-25P5WT 1.5×10 AA30S05-01P5CC
2.0×10
（ ODS
2.0× 75 AA30S05-L502WT AA30S05-0102CC
2.0×150 AA30S05-1502WT 4.0×23 AA30S05-G304CC
2.0×250 AA30S05-2502WT A guard cartridge holder will need to be purchased separately before using this
）
4.6× 75 AA30S05-L546WT product for the first time.
4.6×100 AA30S05-1046WT
Cartridge holder
4.6×150 AA30S05-1546WT
4.6×250 AA30S05-2546WT Semi-micro guard cartridge holder
XPCHSMW
4.6×300 AA30S05-3046WT (inner diameter: 1.0, 1.5, or 2.0 mm)
6.0×100 AA30S05-1006WT
Cartridge holder
6.0×150 AA30S05-1506WT XPCHW
6.0×250 AA30S05-2506WT (inner diameter: 4.0 mm)
6.0×300 AA30S05-3006WT
S-10 120 4.6×100 AA12S11-1046WT
4.6×150 AA12S11-1546WT
4.6×250 AA12S11-2546WT
4.6×300 AA12S11-3046WT S-3 120 4.0×23 AA12S03-G304CCPK
6.0×100 AA12S11-1006WT
S-5 120 4.0×23 AA12S05-G304CCPK
6.0×150 AA12S11-1506WT
6.0×250 AA12S11-2506WT A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge
6.0×300 AA12S11-3006WT column, and a column coupler (produced by PEEK).
300 4.6×100 AA30S11-1046WT

4.6×150 AA30S11-1546WT
4.6×250 AA30S11-2546WT Guard columns
4.6×300 AA30S11-3046WT
S-3 120 4.0×10 AA12S03-0104WFG
6.0×100 AA30S11-1006WT
5.0×10 AA12S03-0105WFG
6.0×150 AA30S11-1506WT
S-5 120 4.0×10 AA12S05-0104WFG
6.0×250 AA30S11-2506WT
5.0×10 AA12S05-0105WFG
6.0×300 AA30S11-3006WT
10×30 AA12S05-0310WTG
20×50 AA12S05-0520WTG
Semi-preparative columns 4.0×10 AA20S05-0104WFG
200
Particle size Pore size Column size 20×50 AA20S05-0520WTG
Product number
（µm）（Å） inner diameter×length（mm） 300 4.0×10 AA30S05-0104WFG
10×150 AA12S05-1510WT 5.0×10 AA30S05-0105WFG
S-5 120
10×30 AA30S05-0310WTG
10×250 AA12S05-2510WT
10×300 AA12S05-3010WT 20×50 AA30S05-0520WTG
20×100 AA12S05-1020WT S-10 120 4.0×10 AA12S11-0104WFG
20×150 AA12S05-1520WT 5.0×10 AA12S11-0105WFG
20×250 AA12S05-2520WT 10×30 AA12S11-0310WTG
20×150 AA20S05-1520WT 20×50 AA12S11-0520WTG
200
20×250 AA20S05-2520WT 200 4.0×10 AA20S11-0104WFG
10×150 AA30S05-1510WT 5.0×10 AA20S11-0105WFG
300
10×250 AA30S05-2510WT 20×50 AA20S11-0520WTG
10×300 AA30S05-3010WT 300 4.0×10 AA30S11-0104WFG
20×150 AA30S05-1520WT 5.0×10 AA30S11-0105WFG
20×250 AA30S05-2520WT 10×30 AA30S11-0310WTG
20×50 AA30S11-0520WTG
45
第01章.indd 45 11.1.28 10:41:24 AM

Analytical columns
YMC-Pack ODS-AM
● Similar to ODS-A in selectivity ■Pore size：120 Å
● Excellent reproducibility ■Carbon content：17％
● Useful for quality control purposes ■Usable pH range：2.0 〜 7.5
ODS with outstanding lot-to-lot reproducibility

YMC-Pack ODS-AM is a product which especially and surface modifying processes, in order to ensure
emphasizes lot-to-lot reproducibility of the packing stable quality. Double endcapping is conducted to
material. Strict control is performed on all aspects of ensure that lot-to-lot reproducibility is maintained.
quality, including physical properties of silica gel base
Special attention is given to lot-to-lot reproducibility

10
The separation factor (α) of methylparaben/2,6-
dimethylpyridine for 10 lots of ODS-AM packing material
is plotted on the left graph. Excellent lot-to-lot
α (Methylparben/2,6-Dimethylpyridine)
8 reproducibility is achieved even in separation of basic

compounds.
6
（ ODS
4
）
0
1 2 3 4 5 6 7 8 9 10
Lot Number
Acidic compounds Coordination compounds

1 1. Uracil 1. Uracil
2. Benzoic acid 2. Acetylacetone
3. p-Toluic acid 3. 8-Quinolinol
4. p-Ethylbenzoic acid
5. Methy benzoate
ODS-AM ODS with a high degree of
metal impurities
3
3 1
2
2 1
2
5
Secondary interaction of the stationary phase surface may The peak shapes of coordination compounds, including
affect peak shapes of acidic compounds. 8-quinolinol and acetylacetone, may be affected by the
ODS-AM can provide chromatograms without tailing peaks as presence of metal impurities on the surface of the silica. The
shown above. distribution level of metal impurities on the column is strictly
controlled for ODS-AM, providing excellent peak symmetry.
46
第01章.indd 46 11.1.28 10:41:26 AM

Application （G910523J）
Alkaloids
1 1. Hordenine sulfate
2. Pilocarpine chloride
3. Scopolamine bromide
4 7 4. Atropine sulfate
3 5. Cinchonine
6. Sparteine sulfate
5 7. Quinine

2 8. Papaverine chloride 1
9 9. Narcotine chloride
8
Column ：YMC-Pack ODS-AM (5 µm, 120 Å)

　150×4.6 mmI.D.
Eluent ：acetonitrile/20 mM KH2PO4 (20/80)
0 20 40 60 80 min
（ ODS
Analytical columns Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
）
S-3 120 2.0× 35 AM12S03-H502WT S-3 120 2.0×10 AM12S03-0102CC
2.0× 50 AM12S03-0502WT 4.0×23 AM12S03-G304CC
2.0× 75 AM12S03-L502WT S-5 120 1.0×10 AM12S05-0101CC
2.0×100 AM12S03-1002WT 1.5×10 AM12S05-01P5CC
2.0×150 AM12S03-1502WT 2.0×10 AM12S05-0102CC
3.0× 50 AM12S03-0503WT 4.0×23 AM12S05-G304CC
3.0×100 AM12S03-1003WT A guard cartridge holder will need to be purchased separately before using this
3.0×150 AM12S03-1503WT product for the first time.
4.6× 75 AM12S03-L546WT
4.6×100 AM12S03-1046WT
4.6×150 AM12S03-1546WT Cartridge holder
6.0×100 AM12S03-1006WT
6.0×150 AM12S03-1506WT Semi-micro guard cartridge holder
XPCHSMW
6.0×250 AM12S03-2506WT (inner diameter: 1.0, 1.5, or 2.0 mm)
S-5 120 1.0×150 AM12S05-1501WT Cartridge holder
1.0×250 AM12S05-2501WT XPCHW
1.5×150 AM12S05-15P5WT
(inner diameter: 4.0 mm)
1.5×250 AM12S05-25P5WT
2.0× 75 AM12S05-L502WT
2.0×100 AM12S05-1002WT
2.0×150 AM12S05-1502WT
2.0×250 AM12S05-2502WT S-3 120 4.0×23 AM12S03-G304CCPK
3.0×150 AM12S05-1503WT
S-5 120 4.0×23 AM12S05-G304CCPK
3.0×250 AM12S05-2503WT
4.6× 75 AM12S05-L546WT A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge
4.6×100 AM12S05-1046WT
4.6×150 AM12S05-1546WT
4.6×250 AM12S05-2546WT
4.6×300 AM12S05-3046WT Guard columns
6.0×100 AM12S05-1006WT 4.0×10 AM12S03-0104WFG
6.0×150 AM12S05-1506WT
S-3 120
5.0×10 AM12S03-0105WFG
6.0×250 AM12S05-2506WT 4.0×10 AM12S05-0104WFG
6.0×300 AM12S05-3006WT
S-5 120
5.0×10 AM12S05-0105WFG
10×30 AM12S05-0310WTG
Semi-preparative columns 20×50 AM12S05-0520WTG
S-5 120 10×150 AM12S05-1510WT
10×250 AM12S05-2510WT
10×300 AM12S05-3010WT
20×100 AM12S05-1020WT
20×150 AM12S05-1520WT
20×250 AM12S05-2520WT
See P147 for preparative columns other than those listed above. 47
第01章.indd 47 11.1.28 10:41:27 AM

Analytical columns
YMC-Pack ODS-AQ
● Useful for separation of hydrophilic compounds ■Pore size：120 Å, 200 Å
● Can be used with 100% water in mobile phase ■Carbon content：14％ , 10%
● Different selectivity from conventional ODS ■Usable pH range：2.0 〜 7.5
Hydrophilic ODS
YMC-Pack ODS-AQ has moderate hydrophobicity and It is useful in fields including carbohydrate chemistry for
hydrogen-bonding capacity. It shows different retention oligosaccharides and glycosides, pharmacognosy and
behavior from that of YMC-Pack ODS-A, for samples natural product chemistry.
with relatively high hydrophilicity.
Useful for separation of sugars
This product is useful for separations using mobile

phases in which water content is relatively high, such as
separation of sugars and glycosides.
G11
G12
（ ODS
G13 1. Maltoundecaose（G11）
G14 2. Maltododecaose（G12）
）
3. Maltotridecaose（G13）
4. Maltotetradecaose（G14）
G15 5. Maltopentadecaose（G15）
Column ：YMC-Pack ODS-AQ (5 µm, 120 Å)

　150×4.6 mmI.D.
10 20 30 min Temperature ：37 ℃
Detection ：RI, 8×10-6RIU/FS （M910604M）
Hydrogen-bonding capacity
ODS-A ODS-AQ ODS-AL Chromatograms of purine alkaloids are shown on the left.
1 As compared with ODS-A, ODS-AQ and ODS-AL show
1
longer retention of caffeine and theophylline relative to
1 phenol, an internal standard.
2 2
3 2
3 5
4 5 4 3 5
4
1. Trigonelline
2. Theobromine
3. Theophylline
4. Caffeine
5. Phenol
Change of separation factor (α) of caffeine/phenol

1.8 according to temperature change in ODS-AQ, ODS-A
and ODS-AL is plotted on the graph shown on the left.
Although caffeine is considered to have stronger hydrogen-
1.6 ODS-A
α (Phenol/Caffeine)
bonding properties than phenol, the hydrogen-bonding

capacity of stationary phase may affect the α of caffeine/
phenol. The smaller the hydrogen-bonding capacities
1.4
ODS-AQ are, the higher α of phenol/caffeine. ODS-AQ shows
different retention behavior from that of ODS-A since it has
1.2 moderate hydrogen-bonding capacity.
ODS-AL
1.0
3.0 3.1 3.2 3.3 3.4

(60 ℃) 3 -1 (20 ℃)
10 /T,K
48
第01章.indd 48 11.1.28 10:41:30 AM

Application （G910607E）
Crude drugs (glycosides)

1 1. Arbutin
2. Salicin
5
3. Esculine
4. G-Strophantine(Ouabain)
6
34 7 5. Baicalin
2 6. Barbaloin
7. Glycyrrhizin
8 8. Digitoxine
Column ：YMC-Pack ODS-AQ (5 µm, 120 Å)

　250×4.6 mmI.D.
Eluent ：A) methanol/20 mM NH4H2PO4 (5/95)
　B) methanol/20 mM NH4H2PO4 (80/20)
　0-100％ B (0-20 min,linear),100％ B (20-40 min)

0 10 20 30 40 min Detection ：UV at 250 nm 1
S-3 120 2.0× 35 AQ12S03-H502WT S-3 120 2.0×10 AQ12S03-0102CC
2.0× 50 AQ12S03-0502WT 4.0×23 AQ12S03-G304CC
2.0× 75 AQ12S03-L502WT S-5 120 1.0×10 AQ12S05-0101CC
2.0×100 AQ12S03-1002WT 1.5×10 AQ12S05-01P5CC
（ ODS
2.0×150 AQ12S03-1502WT 2.0×10 AQ12S05-0102CC
3.0× 50 AQ12S03-0503WT 4.0×23 AQ12S05-G304CC
200 4.0×23
）
3.0×100 AQ12S03-1003WT AQ20S05-G304CC
3.0×150 AQ12S03-1503WT A guard cartridge holder will need to be purchased separately before using this
4.6×100 AQ12S03-1046WT product for the first time.
4.6×150 AQ12S03-1546WT
6.0×100 AQ12S03-1006WT
6.0×150 AQ12S03-1506WT Cartridge holder
S-5 120 1.0×150 AQ12S05-1501WT
1.0×250 AQ12S05-2501WT Semi-micro guard cartridge holder
XPCHSMW
1.5×150 AQ12S05-15P5WT (inner diameter: 1.0, 1.5, or 2.0 mm)
1.5×250 AQ12S05-25P5WT Cartridge holder
2.0× 75 AQ12S05-L502WT XPCHW
2.0×150 AQ12S05-1502WT
2.0×250 AQ12S05-2502WT
3.0×150 AQ12S05-1503WT
3.0×250 AQ12S05-2503WT
4.6× 75 AQ12S05-L546WT
4.6×100 AQ12S05-1046WT S-3 120 4.0×23 AQ12S03-G304CCPK
4.6×150 AQ12S05-1546WT
S-5 120 4.0×23 AQ12S05-G304CCPK
4.6×250 AQ12S05-2546WT
4.6×300 AQ12S05-3046WT A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge
6.0×100 AQ12S05-1006WT
6.0×150 AQ12S05-1506WT
6.0×250 AQ12S05-2506WT
6.0×300 AQ12S05-3006WT Guard columns
200 4.6×150 AQ20S05-1546WT 4.0×10 AQ12S03-0104WFG
4.6×250 AQ20S05-2546WT
S-3 120
5.0×10 AQ12S03-0105WFG
S-5 120 4.0×10 AQ12S05-0104WFG
Semi-preparative columns 5.0×10 AQ12S05-0105WFG
S-5 120 10×150 AQ12S05-1510WT 10×30 AQ12S05-0310WTG
10×250 AQ12S05-2510WT 20×50 AQ12S05-0520WTG
10×300 AQ12S05-3010WT
200 4.0×10 AQ20S05-0104WFG
20×100 AQ12S05-1020WT 20×50 AQ20S05-0520WTG
20×150 AQ12S05-1520WT S-10 120 10×30 AQ12S11-0310WTG
20×250 AQ12S05-2520WT 20×50 AQ12S11-0520WTG
200 20×150 AQ20S05-1520WT 200 20×50 AQ20S11-0520WTG
20×250 AQ20S05-2520WT
S-10 120 10×150 AQ12S11-1510WT
10×250 AQ12S11-2510WT
10×300 AQ12S11-3010WT
20×150 AQ12S11-1520WT
20×250 AQ12S11-2520WT
200 20×150 AQ20S11-1520WT
20×250 AQ20S11-2520WT
See P146 for preparative columns other than those listed above.
49
第01章.indd 49 11.1.28 10:41:32 AM

Analytical columns
YMC-Pack ODS-AL
● ODS with residual silanol groups ■Pore size：120 Å
● Utilizes secondary interaction caused by silanol groups ■Carbon content：17％
■Usable pH range：2.0 〜 7.5
Non-endcapped ODS
YMC-Pack ODS-AL uses not only hydrophobic When ionic interaction is utilized, it is preferable to use
interaction but also secondary interaction caused by a buffer in the mobile phase to achieve reproducibility of
silanol groups that affect separation. This results in a chromatograms.
different selectivity from conventional ODS columns.
Effects of silanol group
The separation factor (α) of internal standards

ODS-AL ODS-AM
methylparaben/2,6-dimethylpyridine for ODS-AM is
1 1
different from that of ODS-AL, which is not endcapped.
2 3 ODS-AL, which is affected by residual silanol, shows
relatively great retention of pyridines.
2
（ ODS
3
α(4/3)=1.46 α(4/3)=2.80
1. Uracil
）
2. Pyridine
3. 2,6-Dimethylpyridine
4
4. Methylparaben
4
Utilizes residual silanol groups for separation

ODS-AL ODS-AQ The figure shows separation of disinfectants under the
conditions described in the Japanese Pharmacopeia. The
object is to select the column permitting the elution of benzoic
acid, salicylic acid and theophylline, in this order, insuring
2,3 complete separation of these peaks. With ODS-AQ, separation
of salicylic acid and theophylline is incomplete. By contrast,
3 ODS-AL provides excellent separation. Thus, ODS-AL may
provide excellent separation when the separation conditions
1 cannot be optimized on other ODS columns.
1
Disinfectants
1. COOH 2. COOH
2
OH
Benzoic acid Salicylic acid
3. O 4.
H
4 H3C N OH
N
4 O N N
CH 3
Theophylline (I.S.) Phenol
Column ：YMC-Pack ODS-AL and ODS-AQ (5 µm, 120 Å)

　150×4.6 mmI.D.
Eluent ：100 mM KH2PO4-Na2HPO4 (pH 7.0)/methanol (75/25)
0 5 10 15 20 min 0 5 10 15 20 min Flow rate ：0.8 mL/min
(Conditions described in Japanese Pharmacopoeia 15th ed.)
50
第01章.indd 50 11.1.28 10:41:35 AM

Application （G900417B）
Phenacyl esters of bile acids from pig gall

2 1. Cholic acid (CA)
2. Hyodeoxycholic acid (HDCA)
3. Chenodeoxycholic acid (CDAC)

1
1 Column ：YMC-Pack ODS-AL (5 µm, 120 Å)

250×4.6 mmI.D.
（ ODS
0 10 20 30 40 min Detection ：UV at 240 nm
）
S-5 120 1.0×150 AL12S05-1501WT S-5 120 1.0×10 AL12S05-0101CC
1.0×250 AL12S05-2501WT 1.5×10 AL12S05-01P5CC
1.5×150 AL12S05-15P5WT 2.0×10 AL12S05-0102CC
1.5×250 AL12S05-25P5WT 4.0×23 AL12S05-G304CC
2.0×150 AL12S05-1502WT A guard cartridge holder will need to be purchased separately before using this
2.0×250 AL12S05-2502WT product for the first time.
4.6× 75 AL12S05-L546WT
4.6×100 AL12S05-1046WT
4.6×150 AL12S05-1546WT Cartridge holder
4.6×250 AL12S05-2546WT
4.6×300 AL12S05-3046WT Semi-micro guard cartridge holder
XPCHSMW
6.0×100 AL12S05-1006WT (inner diameter: 1.0, 1.5, or 2.0 mm)
6.0×150 AL12S05-1506WT Cartridge holder
6.0×250 AL12S05-2506WT XPCHW
6.0×300 AL12S05-3006WT
Semi-preparative columns
S-5 120 10×150 AL12S05-1510WT Guard columns
10×250 AL12S05-2510WT
S-5 120 4.0×10 AL12S05-0104WFG
10×300 AL12S05-3010WT
5.0×10 AL12S05-0105WFG
20×100 AL12S05-1020WT
10×30 AL12S05-0310WTG
20×150 AL12S05-1520WT
20×50 AL12S05-0520WTG
20×250 AL12S05-2520WT
See P147 for preparative columns other than those listed above.
51
第01章.indd 51 11.1.28 10:41:36 AM

Analytical columns
J’sphere ODS-H80, ODS-M80, ODS-L80

● Useful for method development ■Pore size：80 Å
● High theoretical plate number ■Carbon content：ODS-H80 22％ , ODS-M80 14% , ODS-L80 9％
■Usable pH range：ODS-H80 1.0 〜 9.0, ODS-M80・L80 2.0 〜 7.5
Three types of ODS, each with different ligand coverage

J’sphere offers a choice from three kinds of ODS made from the same resulting from solute functional groups or tertiary structure.
silica gel base, each with different ligand coverage. J’sphere is useful for the optimization of separation conditions, since
Differences in ligand coverage considerably affect the hydrophobic there is almost no need to consider interactions other than hydrophobic
retention behavior of solutes, as well as the separation behavior and hydrogen-bonding interactions (e.g., ionic or coordinate interaction).
1 Three types of ODS
Image of stationary phase
ODS-H80 ODS-M80 ODS-L80

（ ODS
CnH 2n+1 (n=0-8)

）
Alkylbenzenes
ODS-L80
ODS-M80
Column ：J’sphere
　150×4.6 mmI.D.
ODS-H80 Flow rate ：1.0 mL/min
0 10 20 30 40 50 min Temperature ：37 ℃
Differences in separation characteristics
J’sphere ODS-H80 ODS columns with different

BRAND-B1 16.5 29.6
ligand coverage have different
BRAND-F1 15.9 hydrophobicity, hydrogen
YMC-Pack ODS-AM 15.2 bonding capacity and steric
BRAND-G1 15.2 selectivity for tertiary structures.
J’sphere ODS-M80 15.1 Column ：150×4.6 mmI.D. ODS-H80 has exceptionally high
BRAND-A2 15.0 Eluent ：acetonitrile/water (75/25) hydrophobicity and low hydrogen
BRAND-C3 13.1
Temperature ：37 ℃ bonding capacity, ODS-M80 has
6.2 J’sphere ODS-L80 Detection ：UV at 254 nm moderate hydrophobicity and
hydrogen bonding capacity and
0 10 20 30
k’(octylbenzene) ODS-L80 has low hydrophobicity,
comparable to that of C8 and
0.21 J’sphere ODS-H80
high hydrogen bonding capacity.
BRAND-F1 0.24
J’sphere can be used with
BRAND-G1 0.24
confidence to identify structural
YMC-Pack ODS-AM 0.25 differences of solutes and is useful
BRAND-C3 0.25
for improving the efficiency of
BRAND-B1 0.25 Column ：150×4.6 mmI.D. separation optimization.
J’sphere ODS-M80 0.27 Eluent ：acetonitrile/20 mM KH2PO4 (15/85)
BRAND-A2 0.30
J’sphere ODS-L80 0.30 Detection ：UV at 254 nm
0.20 0.25 0.30

α(caffeine/phenol)
52
第01章.indd 52 11.1.28 10:41:39 AM


Packing Particle size Pore size Column size Packing Particle size Pore size Column size
material （µm）（Å） inner diameter×length（mm） material （µm）（Å） inner diameter×length（mm）
ODS-H80 S-4 80 1.0×150 JH08S04-1501WT ODS-H80 S-4 80 10×150 JH08S04-1510WT
1.0×250 JH08S04-2501WT 10×250 JH08S04-2510WT
1.5×150 JH08S04-15P5WT 20×150 JH08S04-1520WT
1.5×250 JH08S04-25P5WT 20×250 JH08S04-2520WT
2.0× 75 JH08S04-L502WT ODS-M80 S-4 80 10×150 JM08S04-1510WT
2.0×100 JH08S04-1002WT 10×250 JM08S04-2510WT
2.0×150 JH08S04-1502WT 20×150 JM08S04-1520WT
2.0×250 JH08S04-2502WT 20×250 JM08S04-2520WT

1
3.0×150 JH08S04-1503WT ODS-L80 S-4 80 10×150 JL08S04-1510WT
3.0×250 JH08S04-2503WT 10×250 JL08S04-2510WT
4.6× 75 JH08S04-L546WT 20×150 JL08S04-1520WT
4.6×150 JH08S04-1546WT 20×250 JL08S04-2520WT
4.6×250 JH08S04-2546WT
6.0×150 JH08S04-1506WT
6.0×250 JH08S04-2506WT
ODS-M80 S-4 80 1.0×150 JM08S04-1501WT Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)
1.0×250 JM08S04-2501WT ODS-H80 S-4 80 1.0×10 JH08S04-0101CC
1.5×150 JM08S04-15P5WT 1.5×10 JH08S04-01P5CC
1.5×250 JM08S04-25P5WT 2.0×10 JH08S04-0102CC
2.0× 75 JM08S04-L502WT 4.0×23 JH08S04-G304CC
2.0×100 1.0×10
（ ODS
JM08S04-1002WT ODS-M80 S-4 80 JM08S04-0101CC
2.0×150 JM08S04-1502WT 1.5×10 JM08S04-01P5CC
2.0×250 JM08S04-2502WT 2.0×10 JM08S04-0102CC
）
3.0×150 JM08S04-1503WT 4.0×23 JM08S04-G304CC
3.0×250 JM08S04-2503WT ODS-L80 S-4 80 1.0×10 JL08S04-0101CC
4.6× 75 JM08S04-L546WT 1.5×10 JL08S04-01P5CC
4.6×150 JM08S04-1546WT 2.0×10 JL08S04-0102CC
4.6×250 JM08S04-2546WT 4.0×23 JL08S04-G304CC
6.0×150 JM08S04-1506WT A guard cartridge holder will need to be purchased separately before using this
6.0×250 JM08S04-2506WT product for the first time.
ODS-L80 S-4 80 1.0×150 JL08S04-1501WT

1.0×250 JL08S04-2501WT
1.5×150 JL08S04-15P5WT Cartridge holder
1.5×250 JL08S04-25P5WT
2.0× 35 JL08S04-H502WT Semi-micro guard cartridge holder
XPCHSMW
2.0× 75 JL08S04-L502WT (inner diameter: 1.0, 1.5, or 2.0 mm)
2.0×100 JL08S04-1002WT Cartridge holder
2.0×150 JL08S04-1502WT XPCHW
2.0×250 JL08S04-2502WT (inner diameter: 4.0 mm)
3.0×150 JL08S04-1503WT
3.0×250 JL08S04-2503WT
4.6× 75 JL08S04-L546WT
4.6×150 JL08S04-1546WT
4.6×250 JL08S04-2546WT ODS-H80 S-4 80 4.0×23 JH08S04-G304CCPK
6.0×150 JL08S04-1506WT
ODS-M80 S-4 80 4.0×23 JM08S04-G304CCPK
6.0×250 JL08S04-2506WT
ODS-L80 S-4 80 4.0×23 JL08S04-G304CCPK
A guard cartridge pack includes a cartridge holder (1 set), a guard cartridge
Guard columns
ODS-H80 S-4 80 10×30 JH08S04-0310WTG
20×50 JH08S04-0520WTG
ODS-M80 S-4 80 10×30 JM08S04-0310WTG
20×50 JM08S04-0520WTG
ODS-L80 S-4 80 10×30 JL08S04-0310WTG
20×50 JL08S04-0520WTG
53
第01章.indd 53 11.1.28 10:41:40 AM

Analytical columns
YMC-Pack PolymerC18
● Utilizes polymer base which is not affected by silanol ■Usable pH range：2.0 〜 13.0
● Different separation characteristics from silica-based ODS
● Excellent pH stability
P o l y m e r t y p e C18
PolymerC18 is a C18 column made from methacrylate Since π electrons of the carbonyl group or the hydroxyl
polymer. It has excellent pH stability and it is useful for group on the surface of the base material show particular
separation of basic compounds because there are no interactions with solutes, PolymerC18 can show different
silanol or metal impurities to cause secondary interaction. separation characteristics from silica-based ODS.
Can be used with alkali mobile phase

1. Barbital
2. Phenobarbital
1 3 3. Pentobarbital
4. Hexobarbital
5. Secobarbital
4
2
5
（ ODS
）
Column ：YMC-Pack PolymerC18

　150×4.6 mmI.D.
Eluent ：50 mM Na2HPO4-Na3PO4(pH 11.0)/methanol (75/25)
0 10 20 min Temperature ：30 ℃
Excellent resistance to acid and alkali

Alkali resistance Acid resistance
1 1 1
1
〈Prior to eluent flow〉〈After 6 hours〉

〈Prior to eluent flow〉〈After 100 hours〉
2 2 2
3 3 2
3 3
0 10 20 min 0 10 20 min 0 10 20 min 0 10 20 min
〈Flow conditions〉〈Flow conditions〉

Column ：YMC-Pack PolymerC18 150×4.6 mmI.D. Column ：YMC-Pack PolymerC18 150×4.6 mmI.D.
Eluent ：0.1N NaOH (pH 13)/acetonitrile (80/20) Eluent ：100 mM H3PO4 (pH 1.7)
Temperature ：ambient Temperature ：ambient
Time ：6 hours Time ：100 hours
〈Analytical conditions〉〈Analytical conditions〉
Eluent ：acetonitrile/water (60/40) Eluent ：acetonitrile/water (60/40)
Sample ：1.Uracil Sample ：1.Uracil
2.Methyl benzoate 2.Methyl benzoate
3.Butyl benzoate 3.Butyl benzoate
54
第01章.indd 54 11.1.28 10:41:43 AM

Different separation characteristics from silica-based ODS (aromatic compounds)
YMC-Pack PolymerC18 YMC-Pack ODS-A Polyphenol
2
1. OH 2. OH 3. OH
3 HO OH OH
Phloroglucinol Resorcinol Phenol

2
1

1 1
3
Column ：YMC-Pack PolymerC18 and YMC-Pack ODS-A

　150×6.0 mmI.D.
0 10 20 30 min 0 5 10 min Detection ：UV at 280 nm
Different separation characteristics from silica-based ODS (unsaturated compounds)
（ ODS
YMC-Pack PolymerC18 YMC-Pack ODS-AM
Triterpene saponins
）
1.
2. 19
18
1,2 COO-β-D-Glc COO-β-D-Glc

1 HO
HO
HO HO
CH2 OH CH2 OH
Column ：YMC-Pack PolymerC18 and YMC-Pack ODS-AM

　150×4.6 mmI.D.
Eluent ：methanol/water (45/55) for PolymerC18
　methanol/water (50/50) for ODS-AM
0.00 7.50 15.00 22.50 min 22.50
0.00 7.50 15.00 min
Analytical columns Guard cartridge columns (with inner diameter 2.0 mm: 2/pack, 4.0 mm: 3/pack)
Particle size Column size Particle size Column size
（µm） inner diameter×length（mm）（µm） inner diameter×length（mm）
S-6 2.0× 75 PC99S06-L502WT S-6 2.0×10 PC99S06-0102CC
2.0×150 PC99S06-1502WT 4.0×23 PC99S06-G304CC
4.6×150 PC99S06-1546WT A guard cartridge holder will need to be purchased separately before using this
4.6×250 PC99S06-2546WT product for the first time.
6.0×150 PC99S06-1506WT
Cartridge holder
6.0×250 PC99S06-2506WT
S-10 4.6×250 PC99S10-2546WT Semi-micro guard cartridge holder
XPCHSMW
4.6×300 PC99S10-3046WT (inner diameter: 1.0, 1.5, or 2.0 mm)
6.0×250 PC99S10-2506WT
Cartridge holder
6.0×300 PC99S10-3006WT XPCHW
Semi-preparative columns
Guard columns
S-6 10×250 PC99S06-2510WT
S-6 4.0×10 PC99S06-0104WFG
S-10 10×250 PC99S10-2510WT
5.0×10 PC99S06-0105WFG
20×300 PC99S10-3020WT
10×30 PC99S06-0310WTG
S-10 4.0×10 PC99S10-0104WFG
5.0×10 PC99S10-0105WFG
10×30 PC99S10-0310WTG
20×50 PC99S10-0520WTG
55
第01章.indd 55 11.1.28 10:41:47 AM

第01章.indd 56 11.1.28 10:41:47 AM

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YMC GENERAL CATALOG

Types and characteristics of C18

YMC-Triart C18 16∼23

YMC-Pack ODS series

YMC-Pack PolymerC18 54,55

第01章.indd 13 11.1.28 10:39:03 AM

Types of C18 packing materials

Hybrid silica YMC-Triart C18

1 YMC-Pack ODS series

Types and characteristics of C18 (ODS) packing materials

YMC-Triart C18 2 ・Versatile hybrid silica based

UltraHT series ・2 µm-ODS column for Ultra-

第01章.indd 14 11.1.28 10:39:04 AM

Reversed-Phase C18 Columns

Low molecular Chemical structures diﬀer YMC-Pack Pro C18 RS

Hydrophobicity diﬀers YMC-Pack ODS-A (Wide-Pore)

High molecular High molecular weight peptides YMC-Pack ODS-A (Wide-Pore)

第01章.indd 15 11.1.28 10:39:05 AM

Rugged, long lasting, hybrid particle technology

● Great chemical durability Long column lifetime over wide pH range.

Low cost per analysis.

● Lower back pressure A

YMC-Triart C18 －Integration of triple artistic technologies－

YMC-Triart C18 is a multi-layer hybrid particle consisting of two types

第01章.indd 16 11.1.28 10:39:08 AM

Reversed-Phase C18 Columns

Reaction image for "multi-stage endcapping" Evaluation of silanol activity

40 Peak tailing for basic compounds

Column ：5 µm, 150×3.0 mmI.D.

第01章.indd 17 11.1.28 10:39:14 AM

Quality Control －Excellent reproducibility－

1. Chlorpheniramine 2. Dextromethorphan 1. Hinokitiol

Column ：5 µm, 150×3.0 mmI.D. Column ：5 µm, 150×3.0 mmI.D.

Detection ：UV at 235 nm Detection ：UV at 254 nm

Theoretical plate number

Low back-pressure －Free from pressure problem－ Standard ODS －Best balance hydrophobicity－

Eluent : MeOH/W (80/20) [amylbenzene], MeOH/W (30/70) [caffeine, benzene]

Flow rate ：1.0 mL/min

Conventional hybrid silica based ODS columns tend to be less

第01章.indd 18 11.1.28 10:39:17 AM

Phosphate buffer (pH 11.5, 40 ℃) Triethylamine (pH 11.5, 40 ℃)

Rate of theoretical plate number

40% Hybrid-silica based C18 40%

Reversed-Phase C18 Columns

Column ：5 µm, 150×4.6 mmI.D. Column ：5 µm, 150×4.6 mmI.D.

Durability in high temperature

Silica based C18

Silica based C18

40% Silica based C18 40%

20% 20% The columns are kept in acetonitrile/water/TFA (10/90/1), 70 °C,

Reduced column bleed －Operate at high sensitivity－

Column ：5 µm, 50×2.0 mmI.D.

第01章.indd 19 11.1.28 10:39:21 AM

Excellent resolution －Without adsorption and tailing－

Silica based C18

5 µm, 150×4.6 mmI.D.

Q090123F YMC-Triart C18's innovative surface modification

Analysis of coordination compounds Hinokitiol

Q090925G Hinokitiol Methyl benzoate

YMC-Triart C18 2 ・Versatile hybrid silica based

UltraHT series ・2 µm-ODS column for Ultra-

　Low cost per analysis.

YMC-Triart C18　－Integration of triple artistic technologies－

Quality Control　－Excellent reproducibility－

Low back-pressure　－Free from pressure problem－ Standard ODS　－Best balance hydrophobicity－

Reduced column bleed　－Operate at high sensitivity－

Excellent resolution　－Without adsorption and tailing－

Improvement of loadability　－Minimizing strong solvent/sample loading effects－

1 Column ：YMC-Triart C18 (5 µm, 120 Å)