06

Reversed-Phase
C18 Columns (ODS)

Types and characteristics of C18 packing materials -- 78

Guides for selecting C18 packing materials -- 79
Features of Pro series ODS columns --- 80~82
YMC-UltraHT --------------------------------------- 83
YMC-Pack Pro C18------------------------------- 84
Hydrosphere C18--------------------------------- 85
YMC-Pack Pro C18 RS -------------------------- 86
YMC-Pack ODS-A -------------------------------- 87
YMC-Pack ODS-AM------------------------------ 87
YMC-Pack ODS-AQ ------------------------------ 88
YMC-Pack ODS-AL ------------------------------ 88
J’ sphere ODS-H80, ODS-M80, ODS-L80-- 89
YMC-Pack PolymerC18 ------------------------- 89
Ordering Information ----------------------- 90~92
 06 Reversed-Phase C18 Columns (ODS)

Types and characteristics of C18 packing materials

Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications. YMC offers packing materials with an impressive
assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety. We also offer
hybrid-silica based C18 packing materials that are widely applicable to a variety of fields, from microanalysis to large-scale isolation.

Types of C18 packing materials

Base Products
YMC-Triart C18
Hybrid silica
YMC-Triart C18 ExRS
Core-Shell type silica Meteoric Core
YMC-Pack Pro C18
Hydrosphere C18
Silica YMC-Pack Pro C18 RS
YMC-Pack ODS series
J’sphere ODS series
Polymer YMC-Pack PolymerC18
Reversed-Phase C18 Columns (ODS)

Types and characteristics of C18 packing materials

Pore Particle Silanol Usable
Product name size size C% Characteristics Pages
treatment pH range
(Å) (μm)
Versatile hybrid silica based ODS column
C18 120 20 Great chemical durability 59~61
1.9 Suitable as a first choice column
YMC-Triart 3 Yes 1.0~12.0 Versatile hybrid silica based ODS column
5 Great chemical durability
C18 ExRS 80 25 62
Excellent selectivity of isomers and
structural analogs
Core-Shell type ODS
C18 80 7
Ultra fast analysis and excellent
Meteoric Core 2.7 Yes 1.5~10.0 72~75
resolution
C18 BIO 160 5
Superior separation of basic compounds
2
3 Processed with YMC CO., LTD.’s
Pro C18 120 16 advanced endcapping technology 83, 84
5 Superior separation of basic compounds
10 2.0~8.0
2 Superior separation of hydrophilic
Pro series Yes compounds
Hydrosphere C18 120 3 12 83, 85
Can be used with 100% water mobile
5 phase
3 Highly durable ODS
Pro C18 RS 80 22 1.0~10.0 Superior separation of basic compounds 86
5 and hydrophobic compounds
3
120 5 17 Currently in use worldwide
10
5
ODS-A 200 12 87
10
ODS with wide pore size
3
For separation of peptides and proteins
300 5 7
10 Yes
YMC-Pack ODS series 2.0~7.5
3
ODS-AM 120 17 Outstanding lot-to-lot reproducibility 87
5
3
120 5 14
Superior separation of hydrophilic
ODS-AQ 10 88
compounds
5
200 10
10
ODS-AL 120 5 17 No For separation utilizing residual silanol 88
ODS-H80 80 4 22 1.0~9.0 High carbon ODS
J'sphere ODS series ODS-M80 80 4 14 Yes Medium carbon ODS 89
2.0~7.5
ODS-L80 80 4 9 Low carbon ODS
6 —
YMC-Pack PolymerC18 — — 2.0~13.0 Polymer-based ODS 89
10 —

78
Guides for selecting C18 packing materials
YMC offers 15 types of ODS columns, each with unique separation characteristics. The proper selection of packing material is
therefore a key factor in the establishment of efficient separation conditions. Selection of packing materials without sufficient
consideration of the separation characteristics may result in an expense of much time and effort in separating a target material. YMC-
Triart C18 is suitable as a first choice column with excellent durability.

YMC-Triart C18
General organic compounds Meteoric Core C18

Chemical structures differ

YMC-Triart C18 ExRS
Low molecular slightly among solutes
weight compounds

Hydrophobicity differs YMC-Pack ODS-A (Wide-Pore)

Reversed-Phase C18 Columns (ODS)

greatly among solutes J’sphere ODS-L80

YMC-Triart C18 ExRS

Hydrosphere C18
Difficult to separate
YMC-Pack Pro C18 RS
using standard ODS YMC-Pack ODS-AL
YMC-Pack PolymerC18

YMC-Triart C18
Basic compounds YMC-Triart C18 ExRS

YMC-Triart C18
Coordination compounds YMC-Triart C18 ExRS

YMC-Triart C18
Sugars and glycosides Hydrosphere C18

YMC-Triart C18
High molecular Hydrophilic compounds, high molecular
Meteoric Core C18 BIO
weight compounds weight peptides and oligonucleic acids YMC-Pack ODS-A (Wide-Pore)

YMC-Triart C18
Alkaline mobile phase YMC-Triart C18 ExRS
YMC-Pack PolymerC18

79
 06 Reversed-Phase C18 Columns (ODS)

Features of Pro series ODS columns

Pro series high performance reversed-phase columns feature advanced endcapping technology and quality assurance which is ready
for validation. The Pro series includes three kinds of ODS, each with unique separation characteristics. We provide products with
excellent performance and stable quality all over the world to fulfill the needs of the pharmaceutical industry, in which production, as
well as research and development, are increasingly globalized.

Providing products with stable quality all over the world

Features
Silica gel base, low in metal impurities
Processed with advanced endcapping technology
Superior separation of basic compounds
Useful for LC/MS
Excellent durability
Excellent reproducibility
Two types of test reports are included with each column
Reversed-Phase C18 Columns (ODS)

Standard ODS column Low carbon ODS column, High carbon ODS column,
with high versatility useful for separation of useful for separation of highly
hydrophilic compounds hydrophobic compounds
YMC-Pack Pro C18 Hydrosphere C18 YMC-Pack Pro C18 RS
YMC-Pack Pro C18 is a high performance Hydrosphere C18 is an ODS column YMC-Pack Pro C18 RS is a high
monofunctional ODS column providing designed to maintain maximum carbon trifunctional ODS bonding,
standard hydrophobicity, high resolution, hydrophilicity without activating the characterized by high resolution and
high durability and excellent reproducibility. silica surface. It can be used with high durability. It is applicable to a
It is highly versatile in almost all fields, 100% water mobile phase, a problematic wide range of compounds. The
including pharmaceutical products, mobile phase for conventional ODS separation of compounds with relatively
agricultural chemicals, foods, and natural columns. Hydrosphere C18 is useful for high hydrophobicity and those that differ
products. separating hydrophilic compounds. only slightly in hydrophobicity, such as
structural isomers, is outstanding. It
also has excellent resistance to acid
and alkali, making it useful under
demanding separation conditions.

Specifications
Pro C18 Hydrosphere C18 Pro C18 RS
Particle size (μm) 2 , 3 , 5 , 10 2,3,5 3,5
Pore size (Å) 120 120 80
Specific surface area (m2/g) 330 330 510
Carbon content 16% 12% 22%
Bonding monofunctional monofunctional trifunctional
Usable pH range 2.0~8.0 2.0~8.0 1.0~10.0
Characteristics Standard type Separation for hydrophilic compounds High carbon ODS

80
Three kinds of ODS, each with different separation characteristics
The three kinds of ODS columns in the Pro series each have unique carbon content and separation characteristics, including
hydrophobicity, shape recognition ability, and hydrogen-bonding capacity. Selection according to the analyte and mobile phase
of Pro C18 with standard hydrophobicity, Pro C18 RS with high hydrophobicity, or Hydrosphere C18 with low hydrophobicity, facilitates
method development.

Hydrophobicity and shape recognition ability of Pro series ODS

YMC-Pack Pro C18 Pro C18

YMC-Pack Hydrosphere C18
Hydrosphere C18 YMC-Pack ProYMC-Pack
C18 RS Pro C18 RS

1 3
1 1
3
5
2
2 5 3
4 2
4 5
4

Reversed-Phase C18 Columns (ODS)

0 5 10 15 20 min 0 5 10 15 20 min 0 5 10 15 20 25 min
J020805B J020805D J020805E

1. Uracil
Column size : 150 X 4.6 mmI.D.
2. n-Butylbenzene
Eluent : methanol/water (80/20)
3. o-Terphenyl
Flow rate : 1.0 mL/min
4. n-Amylbenzene
Temperature : 37°C
5. Triphenylene
Detection : UV at 254 nm

UltraHT series applicable for conventional LC system

50 X 3.0 mmI.D. 50 X 2.0 mmI.D.

Conventional semi-micro LC system Conventional semi-micro LC system
(cell volume 2.5 µL, path length 5 mm) (cell volume 2.5 µL, path length 5 mm)
Flow rate : 0.8 mL/min Flow rate : 0.36 mL/min
mAU 1 mAU
Injection : 2 µL Injection : 1 µL
2
80 3 80 1 3 N(1) = 3700
N(1) = 6300 2
60 N(2) = 6300 60 N(2) = 4100
4
N(3) = 7500 4 N(3) = 6100
40 40
N(4) = 7800 N(4) = 6900
20 Rs(1, 2) = 2.21 20 Rs(1, 2) = 1.61

0 0 (N070608E)
(N070606B)

0 1 2 3 min 0 1 2 3 min

Conventional LC system Conventional LC system

(cell volume 8 µL, path length 10 mm) (cell volume 8 µL, path length 10 mm)
Flow rate : 0.8 mL/min Flow rate : 0.36 mL/min
mAU mAU Injection : 1 µL
Injection : 2 µL

150 1 3 N(1) = 3500 150 N(1) = 1400

2
N(2) = 3800 3 N(2) = 1500
100 12 N(3) = 3600
100 4 N(3) = 6100 4
N(4) = 6800 N(4) = 4500
50 50
Rs(1, 2) = 1.61 Rs(1, 2) = 0.90
0 0 (N070608B)
(N070606E)

0 1 2 3 min 0 1 2 3 min

Cephalosporin antibiotics
YMC-UltraHT series can be used for fast analysis with conventional 1. Cephalexin
LC system, as its pressure is lower than commercial sub-2 μm columns. Column : YMC-UltraHT Pro C18
2. Cefaclor
Considering the extra column band broadening, the column of 3.0 mml.D. is Eluent : acetonitrile/20 mM KH2PO4
3. Cephaloglycin
more applicable for conventional LC system than the column of 2.0 mml.D.. (10/90)
4. Cephaloridine
Temperature : 37°C
Detection : UV at 260 nm

81
 06 Reversed-Phase C18 Columns (ODS)

Excellent lot-to-lot reproducibility

batch-to-batch
A three-lot comparison is shown on the left.
Test ATest A Test B
Test B Excellent reproducibility is achieved in
separating hydrophilic, basic and acidic
1. Uracil 1. Caffeine
compounds, as well as hydrophobic
2. 4-n-Butoxybenzoic acid 2. Pyridine
3. Ethylbenzene 3. Phenol compounds.
4. n-Propylbenzene

Lot C Lot C

Lot B Lot B

Lot A Lot A

0 5 10 15 min 0 4 8 min
Reversed-Phase C18 Columns (ODS)

column-to-column
Advanced packing technology results in high
150 X 4.6 mml.D. theoretical plate number column efficiency. All packed columns are
24000 inspected and controlled according to the
strictest quality control criteria for column
Theoretical plate number

21000
performance ever, including theoretical plate
18000
number testing.

15000

12000

9000

6000

3000
20 40 60 80 100

Column No.

Two types of test reports

<Packing Material Test Report> <Column Test Report>

Each Pro series includes
both, a Column Test Report
and a Packing Material
Test Report, to certify lot-to-
lot and column-to-column
reproducibility.

This report describes basic physical properties of silica The Column Test Report shows the theoretical plate number
gel, including particle size, pore size and metal content, and tailing factor to indicate column performance. For
carbon loading after modification, and the specifications convenience in deterioration evaluation, this report includes all
and results of separation characteristics tests. analytical conditions including sample concentration as well.

82
 Ultra-Fast LC column

YMC-UltraHT
YMC-UltraHT is a C18 bonded-phase based on highly efficient 2 μm spherical silica particles. YMC-UltraHT series columns
are specifically designed for high-speed and high throughput analysis in various fields such as pharmaceuticals, foods, and
environmentals. YMC-UltraHT has two products. Pro C18 as our standard ODS, and Hydrosphere C18 for hydrophilic
compounds. Both of them show the same selectivity and superior peak shapes as 3 μm and 5 μm Pro series, allowing for an easy
method transfer from conventional HPLC to ultra-fast HPLC without changing elution conditions.

2 μm ODS column ideal for Ultra-Fast LC

Features
Superior column performance at higher flow rate and higher pressure
Reduced analysis time while maintaining excellent resolution
Excellent resolution with back pressure less than that of sub-2 μm
Applicable to both conventional HPLC and specific ultra pressure LC system
Same selectivity and superior peak shapes as 3 μm and 5 μm Pro series
Simple method transfer from conventional HPLC on Pro series; no need to change elution conditions

Reversed-Phase C18 Columns (ODS)

Characteristics of 2 μm packing material

Van Deemter curves Column pressure

0.020 80
Optimum flow rate
for 5 µm Optimum flow rate for 2 µm Optimum flow rate for 2 µm Brand I7 1.7 µm
(0.2 mL/min for 2 mmI.D.) 70
(0.3-0.8 mL/min for 2 mmI.D.) (0.3-0.8 mL/min for 2 mmI.D.)
YMC 5 µm Brand L1 1.8 µm
Column pressure (MPa)

0.015 60
HETP (mm/plates)

50
YMC 3 µm
0.010
Brand L1 1.8 µm 40 YMC 2 µm
applicable
YMC 2 µm 30 pressure range
for conventional LC YMC 3 µm
0.005 20
Brand I7 1.7 µm

10 YMC 5 µm

0.000 0
0 1 2 3 4 5 6 7 8 9 10 11 0 1 2 3 4 5 6 7 8 9 10 11
Interstitial linear velocity (mm/sec) Interstitial linear velocity (mm/sec)

YMC-UltraHT series shows nearly the same efficiency as

Column : 50 X 2.0 or 2.1 mmI.D.
sub-2 μm columns for ultra fast LC system and reduces the column
Eluent : acetonitrile/water (60/40)
pressure significantly.
Temperature : 25°C
Sample : butyl benzoate

Specifications
Pro C18 Hydrosphere C18
Particle size (μm) 2
Pore size (Å) 120
2
Specific surface area (m /g) 330
Carbon content 16% 12%
Bonding monofunctional
Usable pH range 2.0~8.0
Characteristics Standard type For polar analytes

83
 06 Reversed-Phase C18 Columns (ODS)

Analytical columns

YMC-Pack Pro C18

Superior separation of basic compounds Pore size : 120 Å
Processed with advanced endcapping technology Carbon content : 16%
Excellent reproducibility Usable pH range : 2.0~8.0
Utilizes highly pure silica gel base USP L1

Perfectly endcapped ODS

YMC-Pack Pro C18 includes more advanced endcapping technology for strictly controlled processing of residual silanol groups that
are likely to affect quality. Pro C18 is highly appropriate for basic compounds, including pharmaceutical products and agricultural
chemicals.

Versatility ODS column in almost all fields

Pro C18 Brand I4 Brand L1

3 4 5 3 4 5 3 4 5 3 4 5 4, 5 4, 5
Reversed-Phase C18 Columns (ODS)

3 3
1

1 1
1 2 1 2
2
2 2

6 6 6 6 6 6
F040119B F040119B F030130C F030130C F040130E F040130E

10 150 205min 10 0 15 5 2010min 150 205min 100 155 10min

20 150 205min 10 15 20 min

Nose drops (OTC) Nose drops (OTC)

1. 2. 1.3. 2.
4. N
3. 4. N
CH3 O CH3 O
CH3 CH3
CHCOOH NHCCH2N(CH2CH3)2
CHCOOH NHCCHCHCH 2CH2N
2N(CH2CH3)2 CH3
CHCH2CH2N
CH3
CHCOOH CH3
H
CHCOOH
N
CH2 CH3
H
N
CH2 Column : 150 X 4.6 mmI.D. (5 μm)
N
Cl
N
Cl Eluent : A) 20 mM KH2PO4-H3PO4 (pH 2.5)
Maleic acid Lidocaine Naphazoline
Maleic acid Chlorpheniramine
Lidocaine Naphazoline Chlorpheniramine B) methanol
20-90%B (0-15 min), 90%B (15-20 min)
5. 6. CH3 5. CH3 CH3 6. CH3 CH3 CH3 Flow rate : 1.0 mL/min
+ +
CH3 C CH3 C Cl3- C CH3 C Cl-
OH
COOCH3 CH3
OH
CH3
(OCH2CH2)2 N CH2
COOCH3 CH3
CH
CH3 CH3
(OCH2CH2)2 N CH2
CH3
Temperature : 37°C
Detection : UV at 260 nm
Methyl p-hydroxybenzoate Benzethonium
Methyl p-hydroxybenzoate
chloride Benzethonium chloride

YMC-Pack Pro C18 is a high performance ODS column providing standard hydrophobicity, high resolution, high durability and excellent reproducibility.
This column is highly appropriate for basic compounds that often elute with poor peak shapes on competitive columns.

Application (A990121B)

Peptides
1. Oxytocin
2. Met-Enkephalin
2
5
3. Leu-Enkephalin
3 4. Angiotensin I
4 5. α-Mating factor
6
1 6. Insulin

Column : YMC-Pack Pro C18 (3 μm, 120 Å)

75 X 4.6 mmI.D.
Eluent : A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
20-40%B (0-20 min, linear)
Flow rate : 1.0 mL/min
Temperature : 37°C
Detection : UV at 220 nm

0 5 10 15 20 min

84
 Analytical columns

Hydrosphere C18
Strong retention of hydrophilic compounds Utilizes highly pure silica gel base Pore size : 120 Å
Can be used with 100% water mobile phase Carbon content : 12%
Superior separation of basic compounds Usable pH range : 2.0~8.0
Excellent reproducibility USP L1

Hydrophilic ODS
Hydrosphere C18 is designed to maintain adequate hydrophilicity on the packing surface for superior separation of hydrophilic compounds.
Hydrophilic compounds are retained with much greater affinity than with conventional ODS columns, permitting the product to be used
with 100% water mobile phase, a problematic mobile phase for conventional ODS columns. Hydrosphere C18 is useful for separating a wide
range of compounds, including nucleic acids and their derivatives, organic acids, saccharides, glycosides and peptides.

Appropriate for separation of hydrophilic compounds

Separation of organic acids

2
2 High hydrophilicity of the
Hydrosphere C18 3,4 ODS column (Brand I)
conventional Hydrosphere C18 packing

Reversed-Phase C18 Columns (ODS)

1
2
material surface enables longer
1 2 retention and superior separation
5 53,4 of citric acid and fumaric acid as
4 1
3 compared to a conventional ODS
1 column (Brand I).
5 5
4
3

1. Lactic acid
Column : 150 X 4.6 mmI.D.
0 5 10 min 0 5 10 min 2. Acetic acid
A000310E A000615A Eluent : 20 mM NaH2PO4-H3PO4 (pH 2.8)
3. Citric acid
Flow rate : 1.0 mL/min
4. Fumaric acid
0 5 10 0 5 10
Temperature : 30°C
min min 5. Succinic acid
A000310E A000615A Detection : UV at 210 nm

Reproducibility of retention time when used with 100% water mobile phase

When conventional ODS columns

Hydrosphere C18 conventional ODS column (Brand II) conventional ODS column (Brand III)
are used with 100% water
3
Before standing

mobile phase, the apparent

3
3 2 hydrophobicity decreases
2 2
1 1 due to repulsive interactions
3 1
Before standing

between water and hydrophobic

3
3 2 5 groups on the surface of the
2 5 4 5
4 2 4
1 1 packing materials, reducing
1
retention of compounds. In the
4 5 experiment illustrated here,
4 5 5
4 columns filled with 100% water
0 10 20 min 0 10 20 min 0 10 20 min mobile phase were left to stand
S0515A S0518F
S0518H overnight. Retention times of
nucleic acid bases were analyzed
0 10 20 min 0 10 20 min before and after standing and
After standing

0 10 20 min
S0515A S0518H S0518F
compared. The retention times of
adenine after standing decreased
After standing

to 89% of the original value for

71% Brand II and to 71% of the original
98% 89%
Brand III value. By contrast,
Hydrosphere C18 retained 98% of
71%
89% its original retention for adenine.
98%
Hydrosphere C18 is designed to
0 10 20 min 0 10 20 min 0 10 20 min maintain adequate hydrophilicity
S0517A S0519A S0519C
on the packing surface so that
After completion of analysis, the flow was stopped and the columns were left to stand overnight (ca. 15 hours). hydration can be achieved, and
0 10 20 min 0 10 20 min 0 10 20 min when Hydrosphere C18 is used
1. CytosineS0517A S0519A S0519C
with mobile phase containing no
Column : 150 X 4.6 mmI.D.
2. Uracil After completion of analysis, the flow was stopped and the columns were left to stand overnight (ca. 15 hours). organic solvent, the retention
Eluent : 20 mM KH2PO4-K2HPO4 (pH 6.9)
3. Guanine time is not significantly shortened
Flow rate : 1.0 mL/min
4. Thymine and highly reproducible
Temperature : 37°C
5. Adenine chromatograms can be obtained.
Detection : UV at 254 nm

85
 06 Reversed-Phase C18 Columns (ODS)

Analytical columns

YMC-Pack Pro C18 RS

Excellent acid resistance and alkaline resistance (pH 1 to 10) Pore size : 80 Å
Superior separation of structural isomers and basic compounds Carbon content : 22%
Excellent reproducibility Usable pH range : 1.0~10.0
Utilizes highly pure silica gel base USP L1

High carbon ODS

YMC-Pack Pro C18 RS is a trifunctional type high carbon ODS column characterized by high resolution and high durability. It is
applicable to a wide range of compounds, providing good separation of basic compounds that easily cause tailing peaks. The
separation selectivity for compounds that differ only slightly in hydrophobicity is outstanding. It also has excellent resistance to acid
and alkali, making it useful under demanding separation conditions.

Nicardipine hydrochloride

Pro C18 RS Pro C18 Hydrosphere C18 The separation of

degradation products of
Reversed-Phase C18 Columns (ODS)

nicardipine hydrochloride,
a compound with relatively
high hydrophobicity, is
shown left. The main
Rs=3.31 Rs=2.10 Rs=2.48 peak and the degradation
products are separated
poorly on competitive
columns. Even if Pro C18
or Hydrosphere C18 is
used, baseline resolution
is difficult. On the other
hand, Pro C18 RS,
superior in hydrophobicity
and the ability to discern
structural differences, can
0 10 20 30 min 0 10 20 min 0 10 20 min
F030120B-2 F030120D-2 F030121A-2 separate the main peak
and degradation products
Brand I4 Brand D4 Brand I1 completely. As seen
here, Pro C18 RS shows
excellent selectivity when
components can elute
very close together in the
Rs=1.55 Rs=1.14 Rs=0.93 separation of compounds
with high hydrophobicity.

0 10 20 min 0 10 20 min 0 10 20 min

F030122D-2 F030121C-2 F030121E-2

H
H3C N CH3
CH2 Column : 150 X 4.6 mmI.D. 5 μm
H3COOC COOCH2CH2N Eluent : 20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (25/75)
CH3
Flow rate : 1.0 mL/min
NO2 .HCl Temperature : 37°C
Detection : UV at 254 nm
Nicardipine HCl

86
 Analytical columns

YMC-Pack ODS-A
Conventional ODS column Pore size : 120, 200, 300 Å
Currently in use worldwide Carbon content : 17%, 12%, 7%
Usable pH range : 2.0~7.5
USP L1

Standard ODS
YMC-Pack ODS-A has a highly endcapped surface structure and appropriate hydrophobicity for separation of a wide range of
compounds. It is produced under strict quality control with respect to 50 or more parameters in order to ensure stable quality. This
product is highly regarded as the standard YMC-Pack packing material in various countries around the world.

Quality control system for excellent performance

The graph indicates lot-to-lot reproducibility with respect
10
to hydrophobic interaction for 10 lots, an essential column
performance characteristic. Extremely stable lot-to-lot
8 reproducibility of the hydrophobic surface is achieved by strict
tR (Butyl benzoate), min

control of variables.
In addition to measurement of the physical properties, a wide
6

Reversed-Phase C18 Columns (ODS)

range of compounds including hydrophobic, acidic, basic and
coordination compounds are analyzed under optimal conditions
4 to evaluate column performance.

0
1 2 3 4 5 6 7 8 9 10
Lot Number

Analytical columns

YMC-Pack ODS-AM
Similar to ODS-A in selectivity Pore size : 120 Å
Excellent reproducibility Carbon content : 17%
Useful for quality control purposes Usable pH range : 2.0~7.5
USP L1

ODS with outstanding lot-to-lot reproducibility

YMC-Pack ODS-AM is a product which especially emphasizes lot-to-lot reproducibility of the packing material. Strict control is
performed on all aspects of quality, including physical properties of silica gel base and surface modifying processes, in order to ensure
stable quality.

Special attention is given to lot-to-lot reproducibility

The separation factor (α) of methylparaben/2,6-
10
dimethylpyridine for 10 lots of ODS-AM packing material
is plotted on the left graph. Excellent lot-to-lot
reproducibility is achieved even in separation of basic
α (Methylparben/2,6-Dimethylpyridine)

8 compounds.

0
1 2 3 4 5 6 7 8 9 10
Lot Number

87
 06 Reversed-Phase C18 Columns (ODS)

Analytical columns

YMC-Pack ODS-AQ
Useful for separation of hydrophilic compounds Pore size : 120, 200 Å
Can be used with 100% water mobile phase Carbon content : 14%, 10%
Different selectivity from conventional ODS Usable pH range : 2.0~7.5
USP L1

Hydrophilic ODS
YMC-Pack ODS-AQ has moderate hydrophobicity and hydrogen-bonding capacity. It shows different retention behavior from
that of YMC-Pack ODS-A, for samples with relatively high hydrophilicity. It is useful in fields including carbohydrate chemistry for
oligosaccharides and glycosides, pharmacognosy and natural product chemistry.

Useful for separation of sugars

This product is useful for separations using mobile phases in which water
content is relatively high, such as separation of sugars and glycosides.

1. Maltoundecaose (G11)
2. Maltododecaose (G12)
G11
3. Maltotridecaose (G13)
Reversed-Phase C18 Columns (ODS)

G12
4. Maltotetradecaose (G14)
5. Maltopentadecaose (G15)
G13
G14
Column : YMC-Pack ODS-AQ (5 μm, 120 Å)
G15 150 X 4.6 mmI.D.
Eluent : methanol/water (5/995)
Flow rate : 1.0 mL/min
Temperature : 37°C
10 20 30 min Detection : RI, 8×10-6RIU/FS
(M910604M)

Analytical columns

YMC-Pack ODS-AL
ODS with residual silanol groups Pore size : 120 Å
Utilizes secondary interaction caused by silanol groups Carbon content : 17%
Usable pH range : 2.0~7.5
USP L1

Non-endcapped ODS
YMC-Pack ODS-AL uses not only hydrophobic interaction but also secondary interaction caused by silanol groups that affect
separation. This results in a different selectivity from conventional ODS columns. When ionic interaction is utilized, it is preferable to
use a buffer in the mobile phase to achieve reproducibility of chromatograms.

Utilizes residual silanol groups for separation

ODS-AL ODS-AQ
Disinfectants The figure shows separation of disinfectants
under the conditions described in the Japanese
1. COOH 2. COOH Pharmacopoeia. The object is to select the
2,3
OH column permitting the elution of benzoic acid,
3 Benzoic acid Salicylic acid salicylic acid and theophylline, in this order,
insuring complete separation of these peaks.
1 3. O 4. With ODS-AQ, separation of salicylic acid and
H
H3C N
N OH theophylline is incomplete. By contrast, ODS-
1 O N N AL provides excellent separation. Thus, ODS-
2 CH 3 AL may provide excellent separation when the
Theophylline (I.S.) Phenol separation conditions cannot be optimized on
other ODS columns.
4

4
Column : YMC-Pack ODS-AL and ODS-AQ (5 μm, 120 Å)
150 X 4.6 mmI.D.
Eluent : 100 mM KH2PO4-Na2HPO4 (pH 7.0)/methanol (75/25)
Flow rate : 0.8 mL/min
Temperature : 30°C
0 5 10 15 20 min 0 5 10 15 20 min Detection : UV at 270 nm
(Conditions described in Japanese Pharmacopoeia 16th ed.)

88
Analytical columns

J’sphere ODS-H80, ODS-M80, ODS-L80

Useful for method development Pore size : 80 Å
High theoretical plate number Carbon content : ODS-H80 22% , ODS-M80 14% , ODS-L80 9%
Usable pH range : ODS-H80 1.0~9.0, ODS-M80·L80 2.0~7.5
USP L1

Three types of ODS, each with different ligand coverage

J’sphere offers a choice from three kinds of ODS made from the same silica gel base, each with different ligand coverage. Differences
in ligand coverage considerably affect the hydrophobic retention behavior of solutes, as well as the separation behavior resulting from
solute functional groups or tertiary structure. J’sphere is useful for the optimization of separation conditions, since there is almost no
need to consider interactions other than hydrophobic and hydrogen-bonding interactions (e.g., ionic or coordinate interaction).

Differences in separation characteristics

ODS columns with different
J’sphere ODS-H80 ligand coverage have different
BRAND-B1 16.5 29.6 hydrophobicity, hydrogen
BRAND-F1 15.9 bonding capacity and steric
YMC-Pack ODS-AM 15.2 selectivity for tertiary structures.

Reversed-Phase C18 Columns (ODS)

BRAND-G1 15.2 Column : 150 X 4.6 mmI.D. ODS-H80 has exceptionally high
J’sphere ODS-M80 15.1 Eluent : acetonitrile/water (75/25) hydrophobicity and low hydrogen
BRAND-A2 15.0 Flow rate : 1.0 mL/min bonding capacity, ODS-M80 has
BRAND-C3 13.1 Temperature : 37°C moderate hydrophobicity and
6.2 J’sphere ODS-L80 Detection : UV at 254 nm hydrogen bonding capacity and
0 10 20 30 ODS-L80 has low hydrophobicity,
k’(octylbenzene) comparable to that of C8 and
high hydrogen bonding capacity.
0.21 J’sphere ODS-H80
J’sphere can be used with
BRAND-F1 0.24
BRAND-G1 0.24
confidence to identify structural
YMC-Pack ODS-AM 0.25 differences of solutes and is useful
BRAND-C3 0.25 Column : 150 X 4.6 mmI.D. for improving the efficiency of
BRAND-B1 0.25 Eluent : acetonitrile/20 mM KH2PO4 (15/85) separation optimization.
J’sphere ODS-M80 0.27 Flow rate : 1.0 mL/min
BRAND-A2 0.30 Temperature : 37°C
J’sphere ODS-L80 0.30 Detection : UV at 254 nm
0.20 0.25 0.30
α (caffeine/phenol)

Analytical columns

YMC-Pack PolymerC18
Utilizes polymer base which is not affected by silanol Usable pH range : 2.0~13.0
Different separation characteristics from silica-based ODS
Excellent pH stability

Polymer type C18

PolymerC18 is a C18 column made from methacrylate polymer. It has excellent pH stability and it is useful for separation of basic
compounds because there are no silanol or metal impurities to cause secondary interaction. Since π electrons of the carbonyl group
or the hydroxyl group on the surface of the base material show particular interactions with solutes, PolymerC18 can show different
separation characteristics from silica-based ODS.

Can be used with alkaline mobile phase

1 3
1. Barbital
2. Phenobarbital
3. Pentobarbital
4. Hexobarbital
4 5. Secobarbital
2

Column : YMC-Pack PolymerC18

150 X 4.6 mmI.D.
Eluent : 50 mM Na2HPO4-Na3PO4 (pH 11.0)/methanol (75/25)
Flow rate : 0.5 mL/min
Temperature : 30°C
0 10 20 min Detection : UV at 254 nm

89
 06 Reversed-Phase C18 Columns (ODS)

Ordering Information -Columns-

YMC-UltraHT Pro C18/YMC-Pack Pro C18

Phase Column I.D. Column length (mm) Guard cartridges
dimension (mm)
50 75 100 150 250 I.D. (mm) 10 mm length
120 Å 2.0 AS12S02-0502WT AS12S02-L502WT AS12S02-1002WT — — — —
2 μm 3.0 AS12S02-0503WT AS12S02-L503WT AS12S02-1003WT — — — —
2.0 AS12S03-0502WT AS12S03-L502WT AS12S03-1002WT AS12S03-1502WT — 2.1 AS12S03-01Q1GC
120 Å 3.0 AS12S03-0503WT AS12S03-L503WT AS12S03-1003WT AS12S03-1503WT — 3.0 AS12S03-0103GC
3 μm 4.6 AS12S03-0546WT AS12S03-L546WT AS12S03-1046WT AS12S03-1546WT —
4.0 AS12S03-0104GC
6.0 AS12S03-0506WT AS12S03-L506WT AS12S03-1006WT — —
2.0 AS12S05-0502WT AS12S05-L502WT AS12S05-1002WT AS12S05-1502WT AS12S05-2502WT 2.1 AS12S05-01Q1GC
3.0 AS12S05-0503WT AS12S05-L503WT AS12S05-1003WT AS12S05-1503WT AS12S05-2503WT 3.0 AS12S05-0103GC
120 Å
4.6 AS12S05-0546WT AS12S05-L546WT AS12S05-1046WT AS12S05-1546WT AS12S05-2546WT
5 μm 4.0 AS12S05-0104GC
6.0 — — — AS12S05-1506WT AS12S05-2506WT
10 — — — AS12S05-1510WT AS12S05-2510WT 10 AS12S05-0110CC

YMC-UltraHT Hydrosphere C18/Hydroshere C18

Phase Column I.D. Column length (mm) Guard cartridges
dimension (mm)
50 75 100 150 250 I.D. (mm) 10 mm length
120 Å 2.0 HS12S02-0502WT HS12S02-L502WT HS12S02-1002WT — — — —
2 μm 3.0 HS12S02-0503WT HS12S02-L503WT HS12S02-1003WT — — — —
Reversed-Phase C18 Columns (ODS)

2.0 HS12S03-0502WT HS12S03-L502WT HS12S03-1002WT HS12S03-1502WT — 2.1 HS12S03-01Q1GC

120 Å 3.0 HS12S03-0503WT HS12S03-L503WT HS12S03-1003WT HS12S03-1503WT — 3.0 HS12S03-0103GC
3 μm 4.6 HS12S03-0546WT HS12S03-L546WT HS12S03-1046WT HS12S03-1546WT —
4.0 HS12S03-0104GC
6.0 HS12S03-0506WT HS12S03-L506WT HS12S03-1006WT — —
2.0 HS12S05-0502WT HS12S05-L502WT HS12S05-1002WT HS12S05-1502WT HS12S05-2502WT 2.1 HS12S05-01Q1GC
3.0 HS12S05-0503WT HS12S05-L503WT HS12S05-1003WT HS12S05-1503WT HS12S05-2503WT 3.0 HS12S05-0103GC
120 Å
4.6 HS12S05-0546WT HS12S05-L546WT HS12S05-1046WT HS12S05-1546WT HS12S05-2546WT
5 μm 4.0 HS12S05-0104GC
6.0 — — — HS12S05-1506WT HS12S05-2506WT
10 — — — HS12S05-1510WT HS12S05-2510WT 10 HS12S05-0110CC

YMC-Pack Pro C18 RS

Phase Column I.D. Column length (mm) Guard cartridges
dimension (mm)
50 75 100 150 250 I.D. (mm) 10 mm length
2.0 RS08S03-0502WT RS08S03-L502WT RS08S03-1002WT RS08S03-1502WT — 2.1 RS08S03-01Q1GC
80 Å 3.0 RS08S03-0503WT RS08S03-L503WT RS08S03-1003WT RS08S03-1503WT — 3.0 RS08S03-0103GC
3 μm 4.6 RS08S03-0546WT RS08S03-L546WT RS08S03-1046WT RS08S03-1546WT —
4.0 RS08S03-0104GC
6.0 RS08S03-0506WT RS08S03-L506WT RS08S03-1006WT — —
2.0 RS08S05-0502WT RS08S05-L502WT RS08S05-1002WT RS08S05-1502WT RS08S05-2502WT 2.1 RS08S05-01Q1GC
3.0 RS08S05-0503WT RS08S05-L503WT RS08S05-1003WT RS08S05-1503WT RS08S05-2503WT 3.0 RS08S05-0103GC
80 Å
4.6 RS08S05-0546WT RS08S05-L546WT RS08S05-1046WT RS08S05-1546WT RS08S05-2546WT
5 μm 4.0 RS08S05-0104GC
6.0 — — — RS08S05-1506WT RS08S05-2506WT
10 — — — RS08S05-1510WT RS08S05-2510WT 10 RS08S05-0110CC

* Guard cartridge holder required, part no. XPGCH-Q1 for 2.1 - 4.0 mmI.D. and XPCHSPW1 for 10 mmI.D.

※ See P.120, 121 for preparative columns other than those listed above.

90
Ordering Information -Columns-

YMC-Pack ODS-A
Phase Column I.D. Column length (mm) Guard cartridges
dimension (mm)
50 75 100 150 250 I.D. (mm) 10 mm length
2.0 AA12S03-0502WT AA12S03-L502WT AA12S03-1002WT AA12S03-1502WT — 2.1 AA12S03-01Q1GC
120 Å 3.0 AA12S03-0503WT — AA12S03-1003WT AA12S03-1503WT — 3.0 AA12S03-0103GC
3 μm 4.6 -— AA12S03-L546WT AA12S03-1046WT AA12S03-1546WT —
4.0 AA12S03-0104GC
6.0 -— — AA12S03-1006WT AA12S03-1506WT —
2.0 — AA12S05-L502WT — AA12S05-1502WT AA12S05-2502WT 2.1 AA12S05-01Q1GC
3.0 — — — AA12S05-1503WT AA12S05-2503WT 3.0 AA12S05-0103GC
120 Å
4.6 — AA12S05-L546WT AA12S05-1046WT AA12S05-1546WT AA12S05-2546WT
5 μm 4.0 AA12S05-0104GC
6.0 — — AA12S05-1006WT AA12S05-1506WT AA12S05-2506WT
10 — — — AA12S05-1510WT AA12S05-2510WT 10 AA12S05-0110CC
2.0 — AA30S05-L502WT — AA30S05-1502WT AA30S05-2502WT 2.1 AA30S05-01Q1GC
300 Å 4.6 — AA30S05-L546WT AA30S05-1046WT AA30S05-1546WT AA30S05-2546WT
4.0 AA30S05-0104GC
5 μm 6.0 — — AA30S05-1006WT AA30S05-1506WT AA30S05-2506WT
10 — — — AA30S05-1510WT AA30S05-2510WT 10 AA30S05-0110CC

YMC-Pack ODS-AM
Phase Column I.D. Column length (mm) Guard cartridges
dimension (mm)
50 75 100 150 250 I.D. (mm) 10 mm length

Reversed-Phase C18 Columns (ODS)

2.0 AM12S03-0502WT AM12S03-L502WT AM12S03-1002WT AM12S03-1502WT — 2.1 AM12S03-01Q1GC
120 Å 3.0 AM12S03-0503WT — AM12S03-1003WT AM12S03-1503WT — 3.0 AM12S03-0103GC
3 μm 4.6 — AM12S03-L546WT AM12S03-1046WT AM12S03-1546WT —
4.0 AM12S03-0104GC
6.0 — — AM12S03-1006WT AM12S03-1506WT AM12S03-2506WT
2.0 — AM12S05-L502WT AM12S05-1002WT AM12S05-1502WT AM12S05-2502WT 2.1 AM12S05-01Q1GC
3.0 — — — AM12S05-1503WT AM12S05-2503WT 3.0 AM12S05-0103GC
120 Å
4.6 — AM12S05-L546WT AM12S05-1046WT AM12S05-1546WT AM12S05-2546WT
5 μm 4.0 AM12S05-0104GC
6.0 — — AM12S05-1006WT AM12S05-1506WT AM12S05-2506WT
10 — — — AM12S05-1510WT AM12S05-2510WT 10 AM12S05-0110CC

YMC-Pack ODS-AQ
Phase Column I.D. Column length (mm) Guard cartridges
dimension (mm)
50 75 100 150 250 I.D. (mm) 10 mm length
2.0 AQ12S03-0502WT AQ12S03-L502WT AQ12S03-1002WT AQ12S03-1502WT — 2.1 AQ12S03-01Q1GC
120 Å 3.0 AQ12S03-0503WT — AQ12S03-1003WT AQ12S03-1503WT — 3.0 AQ12S03-0103GC
3 μm 4.6 — — AQ12S03-1046WT AQ12S03-1546WT —
4.0 AQ12S03-0104GC
6.0 — — AQ12S03-1006WT AQ12S03-1506WT —
2.0 — AQ12S05-L502WT — AQ12S05-1502WT AQ12S05-2502WT 2.1 AQ12S05-01Q1GC
3.0 — — — AQ12S05-1503WT AQ12S05-2503WT 3.0 AQ12S05-0103GC
120 Å
4.6 — AQ12S05-L546WT AQ12S05-1046WT AQ12S05-1546WT AQ12S05-2546WT
5 μm 4.0 AQ12S05-0104GC
6.0 — — AQ12S05-1006WT AQ12S05-1506WT AQ12S05-2506WT
10 — — — AQ12S05-1510WT AQ12S05-2510WT 10 AQ12S05-0110CC

YMC-Pack ODS-AL
Phase Column I.D. Column length (mm) Guard cartridges
dimension (mm)
50 75 100 150 250 I.D. (mm) 10 mm length
2.0 — — — AL12S05-1502WT AL12S05-2502WT 2.1 AL12S05-01Q1GC
120 Å 4.6 — AL12S05-L546WT AL12S05-1046WT AL12S05-1546WT AL12S05-2546WT
4.0 AL12S05-0104GC
5 μm 6.0 — — AL12S05-1006WT AL12S05-1506WT AL12S05-2506WT
10 — — — AL12S05-1510WT AL12S05-2510WT 10 AL12S05-0110CC

* Guard cartridge holder required, part no. XPGCH-Q1 for 2.1 - 4.0 mmI.D. and XPCHSPW1 for 10 mmI.D.

※ See P.120, 121 for preparative columns other than those listed above.

91
 06 Reversed-Phase C18 Columns (ODS)

Ordering Information -Columns-

J'sphere
Phase Column I.D. Column length (mm) Guard cartridges
dimension (mm)
75 100 150 250 I.D. (mm) 10 mm length
2.0 JH08S04-L502WT JH08S04-1002WT JH08S04-1502WT JH08S04-2502WT 2.1 JH08S04-01Q1GC
ODS-H80 3.0 — — JH08S04-1503WT JH08S04-2503WT 3.0 JH08S04-0103GC
80 Å 4.6 JH08S04-L546WT — JH08S04-1546WT JH08S04-2546WT
4 μm 4.0 JH08S04-0104GC
6.0 — — JH08S04-1506WT JH08S04-2506WT
10 — — JH08S04-1510WT JH08S04-2510WT 10 JH08S04-0110CC
2.0 JM08S04-L502WT JM08S04-1002WT JM08S04-1502WT JM08S04-2502WT 2.1 JM08S04-01Q1GC
ODS-M80 3.0 — — JM08S04-1503WT JM08S04-2503WT 3.0 JM08S04-0103GC
80 Å 4.6 JM08S04-L546WT — JM08S04-1546WT JM08S04-2546WT
4 μm 4.0 JM08S04-0104GC
6.0 — — JM08S04-1506WT JM08S04-2506WT
10 — — JM08S04-1510WT JM08S04-2510WT 10 JM08S04-0110CC
2.0 JL08S04-L502WT JL08S04-1002WT JL08S04-1502WT JL08S04-2502WT 2.1 JL08S04-01Q1GC
ODS-L80 3.0 — — JL08S04-1503WT JL08S04-2503WT 3.0 JL08S04-0103GC
80 Å 4.6 JL08S04-L546WT — JL08S04-1546WT JL08S04-2546WT
4 μm 4.0 JL08S04-0104GC
6.0 — — JL08S04-1506WT JL08S04-2506WT
10 — — JL08S04-1510WT JL08S04-2510WT 10 JL08S04-0110CC

YMC-Pack PolymerC18
Reversed-Phase C18 Columns (ODS)

Particle Column I.D. Column length (mm) Guard cartridges

size (mm)
75 100 150 250 I.D. (mm) 10 mm length
2.0 PC99S06-L502WT — PC99S06-1502WT — 2.1 PC99S06-01Q1GC
4.6 — — PC99S06-1546WT PC99S06-2546WT
6 μm 4.0 PC99S06-0104GC
6.0 — — PC99S06-1506WT PC99S06-2506WT
10 — — — PC99S06-2510WT 10 PC99S06-0110CC
4.6 — — — PC99S10-2546WT
4.0 PC99S10-0104GC
10 μm 6.0 — — — PC99S10-2506WT
10 — — — PC99S10-2510WT 10 PC99S10-0110CC

* Guard cartridge holder required, part no. XPGCH-Q1 for 2.1 - 4.0 mmI.D. and XPCHSPW1 for 10 mmI.D.

※ See P.121 for preparative columns other than those listed above.

92