Biotechnology Lab Report-2

Name: Rachel Coley
Biotechnology Lab Report
Directions: Complete each lab procedure in the order it is presented. Be sure

to document your investigation by thoroughly completing each activity, such as
filling in the tables, uploading any necessary photos or drawings, and
answering each question.
DNA Analysis Gizmo

Identify which two frogs are twins by comparing DNA profiles, and record your
results below.
1) Open the “DNA Analysis” Gizmo® from the lesson slide.

2) Drag the first frog icon onto the platform and select DNA Scan.
3) Move the DNA profile to the Comparison Guide and repeat the
process for the remaining frogs.
4) Use the data in the Comparison Guide to determine which two frogs
are twins.
Frogs A and B are twins.
Genetic Engineering Gizmo

Open the “Genetic Engineering” Gizmo® from the lesson slide. Follow the
instructions below to complete each activity.
Pest Resistance Test

1) Select the Play button to observe which larvae are resistant or susceptible to
bacteria.
2) Select one of the petri dishes to indicate which bacterial strain you want to work
with moving forward.
Take a screenshot of the results of the pest resistance test. Note: The screenshot
tool is available under the Tools icon in the upper right of the Gizmo®. Save the
screenshot image file.
Upload your screenshot into the field below.
I chose number 4.
Clone Resistance Gene
1) Select one or more genes from the bacterial genome you selected in the
previous step to insert into the bacterial genome of a strain known to be
unable to kill Lepidoptera sp. larvae.
2) Select the Play button and record your results.
Gene A and C didn’t kill the larvae but Gene B did.
3) Did any of your test cultures show evidence of a resistance gene? How do
you know?
Yes, the cultures showed evidence of a resistant gene because when the
genes were inserted the Lepidoptera sp. was killed. For example, when Gene B was
inserted the Lepidoptera sp. died.
4) Take a screenshot showing the results of a successful resistance gene

clone and insert into the field below.
Genetic Modification of Corn
1) Describe the attribute you desire in your new strain of corn. Why did you
select this attribute?
One attribute is a long stalk of corn and resistance to Lepidoptera sp. I

selected this because these are typical things that are expected in corn. You want
the corn to be attractive for selling purposes and you don’t want bugs the destroy
the crop.
2) Detail an action plan that describes the biotechnology you will use to
create the strain of corn with your chosen attribute. Include each step of
your plan and the tools needed to accomplish it.
irst, you want to choose a promoter that allows transcription in all parts of
F
the plant. I chose promoter #1. You then want to choose the genome that allows
for the new gene to be expressed properly but also give the desired characteristics
like long, yellow corn without larvae. You would need the transformed Plant
genome #5 to accomplish this. Then you want to make sure that the larvae can be
killed without the use of insecticides.
Choose Promoter
1) In the “Genetic Engineering” Gizmo®, click one of the four plants to
chose the promoter you would like to use to express your bacterial gene in a
corn plant.
2) Explain why you selected the promoter you did.
I chose this promoter because it would be GFP was expressed throughout the
entire plant.
Transformation
1) The gene and promoter you selected are inserted into 5 undifferentiated
corn calluses. Check each callus for evidence of gene insertion and
expression.
2) Take a screenshot of a callus showing evidence of successful
transformation and insert into the field below.
Grow GM Corn
1) Check the appropriate boxes next to the control and transformed plant to
indicate which components should be added to each treatment.
With Insecticide:
2) Select the show statistics box to compare the control and transformed
plants. What evidence indicates you successfully transformed the corn plant?
The evidence that showed the successful transformation was number of Lepidoptera
sp in the control versus the transformed plant. Also the corn yield in the control
versus the transformed plant. The control plant had 74 larvae while the
transformed plant only had 2. The control plant had an 8% corn yield and the
transformed plant had a 99% corn yield.
3) Take a screenshot of the results showing evidence of successful

transformation and insert into the field below.
Illustration
1) Create a labeled illustration of your new plant, including details about its unique
physical characteristics and a description of their benefits.
2) Take a screenshot of your diagram and insert into the field below.
The size and shape of ear of corn shows the health and appeals to consumers. The
Leaflike structures around the ear protect it. The stalks adds structure and stability
to the plant. The prop roots keep the plant standing upright. The roots act as a
transport system and move water and nutrients throughout the plant.
Lab Review
1) In the simulation, you inserted a disease-resistance gene into corn. Will
this disease resistance be passed on to future generations of corn plants?
Explain.
Yes, the gene will be passed on if two genetically modified individuals are
used. If the gene isn’t present in both plants the gene will not be inherited. If it is
present the gene will pass on to the next offspring.
2) How did your manipulations of genetic information affect the cellular

functions of the corn plant?
he manipulations of the genetic information caused different traits to be

T
present. The manipulation of the genes allowed for a resistance to larvae. One of
the genes resulted in the death of larvae when they came in contact. Other
functions such as the promoter allowed for transcription in all areas of the plant.
3) What technical challenges could you foresee if you wanted to insert a

gene into the human genome instead of the corn genome?
One problem is that most genes are connected to many traits. Potentially, changing
one gene could drastically affect another trait. Also the possibility of mutations
could affect certain processes. For example, the Belgian blue cattle genetically
modified to increase muscle mass. However, a mutation causes the cow to continue
growing which prevents them from having natural offspring.

Biotechnology Lab Report-2

Uploaded by

Copyright:

Available Formats

Biotechnology Lab Report-2

Uploaded by

Document Information

Original Description:

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Biotechnology Lab Report-2

Uploaded by

Copyright:

Available Formats

Name: Rachel Coley