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    Detailed Lesson Plan in Peroxisome

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    Keanno
    The document provides a detailed lesson plan on peroxisomes. It begins by outlining the learning targets and concepts that will be covered, which include the discovery of peroxisomes, their structure, and how they differ from lysosomes. The lesson then describes how Christian de Duve and colleagues discovered peroxisomes using equilibrium density centrifugation to separate urate oxidase from lysosomes based on its behavior. Students will learn that peroxisomes are bounded by a single membrane and contain enzymes involved in hydrogen peroxide metabolism.

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    Detailed Lesson Plan in Peroxisome

    Uploaded by

    Keanno
    232 views8 pages
    The document provides a detailed lesson plan on peroxisomes. It begins by outlining the learning targets and concepts that will be covered, which include the discovery of peroxisomes, their structure, and how they differ from lysosomes. The lesson then describes how Christian de Duve and colleagues discovered peroxisomes using equilibrium density centrifugation to separate urate oxidase from lysosomes based on its behavior. Students will learn that peroxisomes are bounded by a single membrane and contain enzymes involved in hydrogen peroxide metabolism.

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    The document provides a detailed lesson plan on peroxisomes. It begins by outlining the learning targets and concepts that will be covered, which include the discovery of peroxisomes, their structure, and how they differ from lysosomes. The lesson then describes how Christian de Duve and colleagues discovered peroxisomes using equilibrium density centrifugation to separate urate oxidase from lysosomes based on its behavior. Students will learn that peroxisomes are bounded by a single membrane and contain enzymes involved in hydrogen peroxide metabolism.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    232 views8 pages

    Detailed Lesson Plan in Peroxisome

    Uploaded by

    Keanno
    The document provides a detailed lesson plan on peroxisomes. It begins by outlining the learning targets and concepts that will be covered, which include the discovery of peroxisomes, their structure, and how they differ from lysosomes. The lesson then describes how Christian de Duve and colleagues discovered peroxisomes using equilibrium density centrifugation to separate urate oxidase from lysosomes based on its behavior. Students will learn that peroxisomes are bounded by a single membrane and contain enzymes involved in hydrogen peroxide metabolism.

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    You are on page 1of 8

    A Detailed Lesson Plan in Cell Biology

    By Keanno Rod M. Peliña (BSEd III – Biological Sciences)

    I. Targets:

    At the end of the lesson, the students will be able to:

    A. discuss the discovery of peroxisome;


    B. describe the structure of peroxisome;
    C. compare and contrast between the lysosome and peroxisome according to its
    structure and function; and
    D. relate the structure of peroxisome to its function.

    I. Learning Task:
    A. Topic: Peroxisomes
    B. Reference/s:
    The world of the Cell, 3rd Edition, Becker et al. pp. 259-263
    https://ww.ncbi.nlm.nih.gov/books/NBK9930/
    www.wikipedia.org/peroxisomes
    https://www.slideshare.net/theshaikh101/peroxisomes-49072655

    C. Concepts:
    1. Peroxisome is an organelle that are bounded by a single membrane and usually
    found ubiquitously in eukaryotic cell but are prominent in mammalian kidney
    and liver cells, in algae and photosynthetic cell in plants and in germinating
    seedlings of plant species that store fat in their seeds; It is also known as
    microbodies are surrounded by a single membrane, and generally have a finely
    granular matrix.
    2. Christian de Duve and his colleagues discovered the peroxisome
    3. Equilibrium density centrifugation is the technique that allowed the urate
    oxidase to a new class of previously undescribed organelles.
    4. Urate oxidase is a marker enzyme of the peroxisome.
    5. Acid phosphatase is a marker enzyme of the lysosome.
    6. Urate oxidase is a hydrogen peroxide forming enzyme.
    7. Catalase is degradation hydrogen peroxide.
    8. The particles of microbodies are usually about 0.2-2.0 µ in diameter.
    9. The functions of peroxisomes are Hydrogen Peroxide Metabolism; Detoxification
    of Harmful and Toxic Compounds; B - Oxidation of Fatty Acids; Metabolism of
    Nitrogen – containing compounds; Breakdown of unusual substances; Form a
    glyoxysomes through the help of the glyoxylate cycle; Photorespiration in the
    peroxisomes of the leaves which serve to metabolize a side product formed
    during photosynthesis.
    D. Materials:
     Image of the two dimensional and three – dimensional structure of the
    peroxisome.
     Manila papers, colored papers, marker, image of the process of
    separation of 2 organelles through equilibrium centrifugation technique,
    image of 3D structure of peroxisome.
    II. Learning Strategies

    Teacher’s Activity Students’ Activity


    A. Preparatory Activities
    1. Greetings
    2. Checking of Attendance

    B. Motivation

    Okay class, identify the organelle (Students will guess to identify the
    based on the given statement. organelle based in the given statement.)

    • It is an organelle that are bounded by


    single membrane.
    • They have a small membranous
    vesicle that contains enzyme.
    • Prominently found in eukaryotic cell.
    • It is similarly alike to the lysosome
    based on their structure and function.

    Clue:
    11 words
    5 vowels
    6 consonants
    Starts with the letter P
    Ends with the letter S

    What is the organelle did you found Peroxisomes


    out based in the given statement?

    Teacher’s Activity Students’ Activity


    C. Lesson Proper

    Class, our lesson for today is all


    about peroxisomes.

    Okay, let us proceed to the


    discovery of peroxisomes.

    The discovery of peroxisomes also


    depended on the apparently
    anomalous behaviour of specific
    enzymes, although a different
    centrifugation technique was used
    in this case.

    Who discovered these organelles by Christian de Duve and his colleagues


    using centrifugation technique?

    Correct, during the early studies of Urate oxidase


    lysosomes, de Duve became aware
    of what kind of marker enzyme is
    that?

    Yes! Urate oxidase can seem to be


    associated with their lysosomal
    fractions, yet was not an acid
    hydrolase which serve as a marker
    enzyme of lysosome.

    Moreover, this enzyme differed


    somewhat from known lysosomal
    enzymes in its behaviour during
    differential centrifugation which
    also kept de Duve from identifying
    it‘s a lysosomal enzyme.

    What kind of technique of Equilibrium density centrifugation


    centrifugation does eventually
    allowed them to assign urate
    oxidase to a new class of previously
    undescribed organelles?

    Correct! In this time, let us know


    how the peroxisome was discovered
    through the separation of lysosome
    and peroxisome by equilibrium
    density centrifugation.

    Class, I will divide you into 2 groups.


    Each group will be given a task to be
    performed.

    Direction:

    Complete the discovery framework (Students’ perform the activity in the class.)
    through summarizing details.
    Red color corresponds to the image
    of the separation of 2 organelles by
    equilibrium density centrifugation
    through jigsaw puzzle.

    Orange color corresponds to the


    detailed procedure in separating 2
    organelles through equilibrium
    density centrifugation.

    Very good class, It’s my turn now,


    I’m going to discuss the enzymes in
    peroxisomes.

    Once this separation achieved, what Catalase and D – amino acid oxidase.
    are the additional enzymes were
    shown to be present in the urate
    oxidase?

    What are the examples of latter Urate oxidase (H2O2 forming enzyme)
    enzymes involve in the discovery of Catalase (degrades H2O2)
    enzyme in this organelle?

    Yes, Because these enzymes has an


    apparent involvement in hydrogen
    peroxide.

    What is this new organelle became Peroxisomes


    known about?

    What is peroxisome? Peroxisome is an organelle that are bounded


    by a single membrane and usually found
    ubiquitously in eukaryotic cell but are
    prominent in mammalian kidney and liver
    cells, in algae and photosynthetic cell in
    plants and in germinating seedlings of plant
    species that store fat in their seeds; It is also
    known as microbodies are surrounded by a
    single membrane, and generally have a
    finely granular matrix.

    Okay, I know that you are already


    familiar about the discovery of
    peroxisomes, so let’s proceed to the
    structure of peroxisomes.

    How long usually is the size of 0.2-2.0 µ in diameter


    peroxisomes?
    In the animal peroxisome, it often Urate Oxidases
    contains a distinctive crystalline
    core in the center, which contains
    what kind of enzyme which oxidizes
    uric acid?

    - This electron micrograph shows


    several peroxisomes in the
    cytoplasm of a rat liver cell. A
    crystalline core is readily visible in
    each microbody. In animal
    microbodies, the cores are almost
    always crystalline urate oxidase.

    In plants, it often presents in Catalase


    peroxisomes which usually consist
    of what kind of enzyme?

    - The tobacco leaf call shown in this


    electron micrograph contains a
    prominent peroxisome/microbody
    with a crystalline core. In plant
    microbodies, the cores are almost
    always crystalline catalase.

    When such cores are present, it is


    easy to identify microbodies as
    peroxisomes since urate oxidase
    and catalase are two of the enzymes
    by which peroxisomes are defined

    *In the absence of a crystalline core,


    however, it is not always easy to
    spot peroxisome ultrastructurally.

    Okay, I know that you are already


    familiar about the structure of
    peroxisomes, so let’s proceed to the
    comparing and contrasting between
    lysosome and peroxisomes

    What are the similarities between - Lysosomes and peroxisomes are


    lysosome and peroxisomes? small membranous vesicles that
    contain enzymes.
    - Enclose residual substances of an
    internal or external origin to break
    them down. 
    - They have a similar morphological
    structure of these two organelle.

    Correct, what are the differences - According to its size the lysosomes
    between these two organelles? are comparatively large in size, while
    peroxisomes are definitely small.
    - According to its enzymes, lysosomes
    contains hydrolase while
    Peroxisomes, on the other hand,
    contain three oxidative enzymes
    such as catalase, D-amino acid
    oxidase, and uric acid/urate oxidase.
    - According to its presence, lysosomes
    are only found in animal cell, while
    peroxisomes are found in all
    eukaryotic cells.
    - According to its composition, the
    lysosomes consists of degradative
    enzymes while peroxisomes consist
    of oxidative enzymes.
    - According to its function, lysosomes
    are responsible for the digestion of
    cells while peroxisomes are
    responsible for the protection of
    cells against hydrogen peroxide.
    - Other functions included, the
    lysosomes are involved in
    endocytosis, autophagy, and
    phagocytosis, while peroxisomes are
    involved in biosynthesis of lipids and
    photorespiration.

    Okay, I know that you are already


    familiar about the similarities and
    differences between lysosome and
    peroxisomes, so let’s proceed to the
    function of peroxisomes.

    Can you give me at least 7 functions - Hydrogen Peroxide Metabolism


    of peroxisomes - Detoxification of Harmful and Toxic
    Compounds
    - B - Oxidation of Fatty Acids
    - Metabolism of Nitrogen – containing
    compounds
    - Breakdown of unusual substances
    - Form a glyoxysomes through the
    help of the glyoxylate cycle.
    - Photorespiration in the peroxisomes
    of the leaves which serves to
    metabolize a side product formed
    during photosynthesis.

    That’s awesome! You already know Yes!


    the structure and function of
    peroxisomes. Since all of you are
    familiar in the peroxisomes and I
    know you’re really understand our
    lesson for today. Am I right?

    III. Generalization:

    Teacher’s Activity Students’ Activity


    Okay class, so I know you are already Yes sir!
    familiar about the structure and
    functions of peroxisomes! Am I right?

    Very Good, please proceed to your


    respective group to perform our final
    activity in this lesson.

    Group 1: (Students’ will perform the activity in the


    Assemble the jigsaw puzzle of 2D and class)
    3D structure of peroxisome and label
    its parts of the structure.

    Group 2:
    Construct a Venn Diagram to find out
    the similarities and differences
    between lysosomes and peroxisomes.
    IV. Evaluation
    Direction: (Multiple Choices)
    Choose the best answer from the options given. Write the letter of your answer
    in ¼ sheet of paper.

    1. Who discovered the peroxisomes?


    a. Christian de Duve c. Matthias Schleiden
    b. J. Rhodes d. Theodore Schwann

    2. This technique of centrifugation was provided by allowing the urate oxidase to a new class of
    previously undescribed organelles?
    a. Differential centrifugation c. Rate-Zonal Density centrifugation
    b. Equilibrium density centrifugation. d. None of the above

    3. How much lower buoyant density does the Triton WR-1339 accumulate the detergent
    preferentially in their lysosomes by giving this organelle?
    a. 1.10 – 1.14 g/cm3
    b. 1.15 – 1.19 g/cm3
    c. 1.20 – 1.25 g/cm3
    d. 1.25 – 1.30 g/cm3

    4. Which of the following is NOT describes the structure of peroxisome?


    a. Bounded by a single membrane
    b. Have a finely granular matrix inside in this organelle
    c. It contains crystalline core in the center which contains enzyme
    d. Prominently found in prokaryotic cell.

    5. All of the following are the functions of animal and plant peroxisomes EXCEPT:
    a. D - Oxidation of Fatty Acids
    b. Detoxification of Harmful and Toxic Compounds
    c. Hydrogen Peroxide Metabolism
    d. Photorespiration

    V. Assignment:
     What is cytoskeleton?
     What are the 3 major structural element of cytoskeleton and differentiate them according
    to its structure.
     What are those function of the 3 major structural element of cytoskeleton?

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