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    270520101543am205 20161

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    Imran Azam
    The document is a lab report for a patient named Imran Azam, age 41, who was referred for a PCR test to detect Hepatitis C virus (HCV) RNA. The test result was negative, meaning HCV RNA was not detected. The method used was a real-time PCR test that can quantitatively detect HCV RNA extracted from plasma/serum. A positive internal control confirms the extraction and amplification process worked properly. Since antibody tests only detect past exposure, molecular detection of HCV RNA by PCR is the gold standard to diagnose a current infection. Quantitative RNA levels provide prognostic information for treatment response monitoring.

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    270520101543am205 20161

    Uploaded by

    Imran Azam
    24 views1 page
    The document is a lab report for a patient named Imran Azam, age 41, who was referred for a PCR test to detect Hepatitis C virus (HCV) RNA. The test result was negative, meaning HCV RNA was not detected. The method used was a real-time PCR test that can quantitatively detect HCV RNA extracted from plasma/serum. A positive internal control confirms the extraction and amplification process worked properly. Since antibody tests only detect past exposure, molecular detection of HCV RNA by PCR is the gold standard to diagnose a current infection. Quantitative RNA levels provide prognostic information for treatment response monitoring.

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    270520101543AM205-20161

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    The document is a lab report for a patient named Imran Azam, age 41, who was referred for a PCR test to detect Hepatitis C virus (HCV) RNA. The test result was negative, meaning HCV RNA was not detected. The method used was a real-time PCR test that can quantitatively detect HCV RNA extracted from plasma/serum. A positive internal control confirms the extraction and amplification process worked properly. Since antibody tests only detect past exposure, molecular detection of HCV RNA by PCR is the gold standard to diagnose a current infection. Quantitative RNA levels provide prognostic information for treatment response monitoring.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    24 views1 page

    270520101543am205 20161

    Uploaded by

    Imran Azam
    The document is a lab report for a patient named Imran Azam, age 41, who was referred for a PCR test to detect Hepatitis C virus (HCV) RNA. The test result was negative, meaning HCV RNA was not detected. The method used was a real-time PCR test that can quantitatively detect HCV RNA extracted from plasma/serum. A positive internal control confirms the extraction and amplification process worked properly. Since antibody tests only detect past exposure, molecular detection of HCV RNA by PCR is the gold standard to diagnose a current infection. Quantitative RNA levels provide prognostic information for treatment response monitoring.

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    Lab No.

    : 10066
    Name: IMRAN AZAM
    205-20161

    Receiving Date: 21/05/2020 Age: 41 Years Gender: Male


    Printing Date: 27/05/2020 Refered By: SELF
    Panel: # 205-20161 (1991)
    A.R. Labs (Gujrat)
    Molecular Biology ____________________________________

    Test Name: PCR - HCV RNA (Quantitative)

    Result HCV RNA IS NOT DETECTED (NEGATIVE)

    Method Used Cepheid Smart Cycler II - Real Time PCR


    HCV Real-Time Quant is a Real-Time test for the Quantitative detection of Hepatitis C Virus in human plasma. HCV RNA is
    extracted from plasma/serum, amplified using real time amplification and detected using fluorescent reporter dye probes
    specific for HCV or HCV IC. Internal Control (IC) serves as an extraction and an amplification control for each individually
    processed specimen and to identify possible inhibition. IC is detected in a channel other than the HCV RNA. Monitoring the
    fluorescence intensities during Real-Time allow the detection and quantification of the accumulating product without having
    to re-open the reaction tube after the real time amplification. Analytical sensitivity of this method is 250 IU/mL.

    patient Sample Graph Positive Control

    comments
    1) Since Elisa and Riba are antibody test, the positivity of either one or both does not necessarily indicate current HCV
    infection, as patients who have recoverd from infection may remain anti-HCV positive for many years.The direct molecular
    biology detection of HCV RNA by reverse-transcriptase polymerase chain reaction (RT-PCR) is considered the gold standard
    for the diagnosis of HCV infection.
    2) Quantitative HCV RNA testing provides prognostic information regarding likelihood of treatment response and it plays an
    important role in monitoring the antiviral response to treatment. Sustained virological response is defined as testing negative
    for HCV RNA 6 months after response to treatment.
    3) The same type of quantitative HCV RNA test should be used throughout a patient's treatment course.

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