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    Lab Activity

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    Rose Ann Bisagar
    1. The document describes Laboratory Experiment No. 1 on the compound microscope. It introduces the importance of microscopes and the four key attributes of magnification, resolution, contrast, and measurement. 2. The objectives are to learn microscope parts and functions, examine prepared slides of protozoans, make a wet mount slide, and determine specimen magnifications. 3. The procedures involve labeling microscope parts, examining letters and hair under low and high power objectives, and drawing protozoan slides to determine magnifications.

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    Lab Activity

    Uploaded by

    Rose Ann Bisagar
    35 views7 pages
    1. The document describes Laboratory Experiment No. 1 on the compound microscope. It introduces the importance of microscopes and the four key attributes of magnification, resolution, contrast, and measurement. 2. The objectives are to learn microscope parts and functions, examine prepared slides of protozoans, make a wet mount slide, and determine specimen magnifications. 3. The procedures involve labeling microscope parts, examining letters and hair under low and high power objectives, and drawing protozoan slides to determine magnifications.

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    LAB-ACTIVITY

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    1. The document describes Laboratory Experiment No. 1 on the compound microscope. It introduces the importance of microscopes and the four key attributes of magnification, resolution, contrast, and measurement. 2. The objectives are to learn microscope parts and functions, examine prepared slides of protozoans, make a wet mount slide, and determine specimen magnifications. 3. The procedures involve labeling microscope parts, examining letters and hair under low and high power objectives, and drawing protozoan slides to determine magnifications.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    35 views7 pages

    Lab Activity

    Uploaded by

    Rose Ann Bisagar
    1. The document describes Laboratory Experiment No. 1 on the compound microscope. It introduces the importance of microscopes and the four key attributes of magnification, resolution, contrast, and measurement. 2. The objectives are to learn microscope parts and functions, examine prepared slides of protozoans, make a wet mount slide, and determine specimen magnifications. 3. The procedures involve labeling microscope parts, examining letters and hair under low and high power objectives, and drawing protozoan slides to determine magnifications.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 7

    LABORATORY EXPERIMENT NO.

    COMPOUND MICROSCOPE

    Introduction

    The microscope is an important laboratory equipment in the study of living things. Much of the
    present knowledge of cellular organization has been made possible by the development of better and more
    powerful microscopes.

    With such importance, this activity highlights the value of knowing the principle of light and optics
    in microscopy. It further emphasizes the four important attributes of the microscope: magnification,
    resolution, contrasts and microscopic measurement. It is in these attributes that the effective use of the
    microscope lies.

    Objectives

    At the end of this activity, the students are expected to:

    1.Label the parts of the compound microscope and their functions


    2.Examine the prepared slides of paramecia, amoeba and other forms of protozoans under LPO and
    HPO
    3.Prepare a wet mount slide
    4.Manipulate the microscope correctly
    5.Determine the magnification of the specimens

    Materials

    1 Compound microscope Prepared slides:paramecia, amoeba and euglena


    2 Glass slide and coverslip
    Dropper
    Specimen (old newspaper)

    Procedure

    A. The Compound Microscope


    1.Label the numbered parts of the compound microscope in Figure 1.1.
    2.Classify each parts of the microscope as to illuminating, magnifying and supporting. Give the functions
    of each numbered parts. Record your observation in Table 1.1.
    3.List down the techniques in handling the microscope.

    B.Microscope Manipulation

    1.Using letter “e” as the specimen

    a.Cut a smallest “e” from an old newspaper.


    b.Place the “e” on the glass slide in an upright position. Cover with a slip.
    Drop a small amounts of water at the side of the slip.
    c.Examine the specimen under LPO. Draw the image as it appears under the
    microscope.
    d.Move the specimen to your right and then to your left. Notice the direction
    of the movement.
    e.After LPO, shift your focus to the HPO. Draw the image. Observe the
    differences.

    2.Using hair strand as the specimen

    a.Make a wet mount of two crossed hair strands and focus under the LPO,
    then HPO.
    b.Draw the image.

    3.Examine the provided prepared slides

    a.Secure the prepared slides ( paramecia, amoeba and euglena) .


    b.Focus the slide under the LPO and HPO. Draw the image.
    c.Determine the magnification of your drawing.
    Name:_____________________________________ DatePerformed:__________
    Course No:______________ Date Submitted:__________
    Day/Time:______________ Rating:________

    Laboratory Worksheet 1

    Compound Microscope

    Observation

    1.Label the numbered parts of the provided diagram of the compound microscope below. Place all the
    labels on Table 1.1.
    2.Table 1.1 The different parts of the microscope, their classification and functions.

    Function
    Numbered Labeled Parts Classification

    Parts (Illuminating,magnifying

    or supporting)

    10

    11

    12

    13

    14

    15
    3.List down the techniques in handling the microscope.

    B.Draw the specimen focused under the LPO and HPO of the microscope.

    1.Using letter “e” as a specimen

    Type of Objective:
    LPO HPO

    Total magnification:
    _______x ______x

    a.What happened to the position of letter “e”?


    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________
    ____________________________________________________________________________
    b.Which among the objectives provided the highest magnifying field?
    _____________________________________________________________________________
    _____________________________________________________________________________
    _____________________________________________________________________________
    _____________________________________________________________________________
    _____________________________________________________________________________

    2.Using hair strand as the specimen

    Type of Objective:
    LPO HPO

    Total magnification:
    _______x ______x

    a.How could you avoid bubbles in the preparation of wet mounts?


    _____________________________________________________________________________________
    _____________________________________________________________________________________
    _____________________________________________________________________________________
    _____________________________________________________________________________________

    b.How would you differentiate a bubble from a true specimen?


    _____________________________________________________________________________________
    _____________________________________________________________________________________
    _____________________________________________________________________________________
    _____________________________________________________________________________________
    3.Using a prepared slides

    PARAMECIUM AMOEBA EUGLENA


    HPO HPO HPO
    Total magnification:
    _______x ______x ______x

    a.Considering the shape of the specimens, was there any change in their shape under LPO and HPO?
    _______________________________________________________________________________
    _______________________________________________________________________________
    _______________________________________________________________________________
    _______________________________________________________________________________

    b.What is the shape of the organisms?


    _______________________________________________________________________________
    _______________________________________________________________________________
    _______________________________________________________________________________
    _______________________________________________________________________________

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