Blood Bank Harr

BLOOD BANK
HARR
BLOOD BANK – IgM – BINDS 10 ADSORPTION-

PHENOTYPING ANTIGENS ELUTION –
CONFIRMATORY
DOSAGE – DUFFY, IgG – BINDS 2 FOR ABO TYPING
RH, MNS ANTIGENS
- ↑ RXN IN NEGATIVE FOR D
HOMOZYGOU SECONDARY – ANTIGEN – RH NEG
S THAN ANAMNESTIC
HETEROZYGO RESPONSE D ANTIGEN – RH
US POSITIVE
DAT – ANTI-IgG,
Fy(a-b-) – 70% OF ANTI-C3d POSITIVE DAT –
AFRICANS POSITIVE WEAK D
GROUP A – A1, A TYPING
Xga – COMMON IN ANTIGENS
WOMEN D-/D-
GROUP A2 – MAY
BOMBAY PRODUCE A1 - ENHANCED D
PHENOTYPE – Oh, hh - STRONGEST
A1 CANT PRODUCE RXN TO ANTI-
MEIOSIS – 1N A D
(AUTOAGGLUTINATI
INTERPHASE – IN ON) D MOSAIC –
BETWEEN CELL PARTIAL D – SERUM
DIVISION BOMBAY – WITH ANTIBODIES
INCOMPATIBLE TO THE MISSING
BLOOD ANTIGENS – WITH O CELLS PARTS OF D
CODOMINANT
BOMBAY – ANTI-A, POSITIVE IAT/DAT –
HARDY WEINBERG ANTI-B, ANTI-H POSITIVE WEAK D
PRINCIPLE
- p2 + 2pq + q2 ACQUIRED B WEAK D TESTING –
ANTIGEN – FOUND IAT AT 37 DEGREES
MALE PASSES Y IN GROUP A
CHROMOSOME TO AB+ - REQUIRE RH
SONS, PASSES X NON-SECRETOR – NEG CONTROL
CHROMOSOME TO POSITIVE
DAUGHTERS INDICATOR CELLS
- Xga BLOOD FOR ABO
GROUP
CHECK CELLS – AUTOANTIBODY – WASHED RBCS –
INSURE PROPER ELUTION – PREVENT
WASHING ANTIBODY PANEL ANAPHYLACTIC
- INSURE ON ELUATE RXNS
ADDITION OF
AHG ROULEAUX + 2 DELAYED HTR –
DROPS SALINE – KIDD
MINOR DISPERSE
CROSSMATCH – TRALI –
OBSOLETE POSITIVE DAT – Igs ANTILEUKOCYTE
- REPLACED BY COAT RBC – ANTIBODY
ANTIBODY ELUTION
PANEL TECHNIQUE FNHR - ↑ I DEGREE
- CANT DO BODY TEMP
AB- PX CAN BE ADSORPTION
TRANSFUSED WITH
A-,B-,O- UNITS BB SPX FOR
CROSSMATCH – 7
O NEGATIVE – DAYS
UNIVERSAL DONOR
PLT – NO
O NEG PX – CROSSMATCHING
TRANSFUSED WITH NEEDED
O NEG UNIT
AUTOLOGOUS
LEAKING UNIT – DONATION – ONLY
SHOULD BE ABO AND RH
DISCARDED TYPING NEEDED
MAJOR ABBREVIATED
CROSSMATCHING – CROSSMATCH –
PX SERUM, DONOR ABO, RH, ANTIBODY
RED CELLS SCREEN,
CROSSMATCH
DONOR WITH
POSITIVE DAT – NO HISTORY OF
DISCARD ABS, ANTIBODY
PANEL NEG – IS
DONOR WITH AN CROSSMATCH CAN
ANTIBODY – KEPT BE PERFORMED
AND PROPERLY
LABELED 90% OF HTS –
CLERICAL ERROR
BLOOD
TYPING
DISCEPANCIES
DISCREPANT BLOOD FORWARD BT – POS Ax – DON’T

TYPE – WASH RBCS REVERSE BT – POS AGGLUTINATE
AND RETYPE AUTOCONTROL POS ANTI-A
IMMUNOSUPPRESSI - COLD - AGGLUTINAT

VE DRUGS - ↓ RBC AUTOANTIBO E ANTI-A,B
ANTIGENS DY - FORM ANTI-
- WASH WITH A1
ANTI-A,B – REACTS WARM
WITH ALL A AND B SALINE
SUBTYPES ANTI-A – 4+
FORWARD BT – NEG ABTI-B – NEG
HYPOGAMMAGLOB REVERSE BT – NEG A1 CELLS – NEG
ULINEMIA, B CELLS – 4+
ACQUIRED B - WEAK AUTOCONTROL – 1+
PHENOMENON – ANTIGEN ANTI-D – 4+
DON’T - INCUBATE AT DAT – NEG
AGGLUTINATE O RT AND 4
CELLS DEGREES - WASH PX CELLS IN
WARM SALINE AND
FORWARD BT – NEG REPEAT RH TYPING
ACQUIRED B REVERSE BT – ONE
ANTIGEN: IS POSITIVE ABO, RH, AB SCREEN
AND CROSSMATCH –
ANTI-A – 4+ - WEAK OF ABNORMAL
ANTI-B – 1+ EXCESSIVE RESULTS -
A1 CELLS – NEG ANTIGEN AUTOANTIBODY
B CELLS – 4+
ANTI-A – MIXED ALL 3 SCREEN
- WEAK ANTI-B FIELD CELLS (+)
RXN ANTI-B – NEG AUTOCONTROL – (- )
A1 CELLS – 3+
B CELLS – 4+ - HIGH
FREQUENCY
- A3 ALLOANTIBO
DY
ANTIGRAM
ANTI-H, ANTI-S – COLD

AUTOANTIBODIES
ANTI-Kpa – LOW FREQUENCY AB
ANTI-k – HGH FREQUENCY

- REACT WITH SCREEN
CELLS
SCREENING CELLS – (+) AT IS (RT)

MAJOR CROSS CROSSMATCH – (+)
(+) AT IS (RT)
AUTOCONTROL – NEG
- COLD ALLOANTIBODY
6 UNITS CROSSMATCHED – 5/6

COMP, 1 INCOMP – POSITIVE DAT
- LOW FREQ ANTIGEN
6 UNITS CROSSMATCHED – 6
UNITS – INCOMP - ↑ FREQ
ANTIGEN
SCREENING CELLS, MAJOR
CROSSMATCH, AUTOCONTROL (+)
- PRESENCE OF
AUTOANTIBODY
IS – 2+
37 DEGREES – 1+
IAT – NEG
- COLD ALLOANTIBODY
COMPONENTS AND
DONORS
I UNIT PRBC VISUAL INSPECTION WAIHA – NO
– BACTERIAL IRRADIATION
- ↑ hgb 1-1.5 g/dl CONTAMINATION REQUIRED
- ↑ hct 3-4% ONLY
LEUKOREDUCED
RBCS, PLASMA – 1-6 FFP – MUST BE RBCS – MUST
DEGREES PREPARED WITHIN 8 RETAIN 85% OF RBC
HRS OF DONOR MASS
PLT – 20-24 DEGREES BLEEDING
- MUST BE GRANULOCYTES –
PREPARED WITH 8 WARMING BLOOD SHOULD BE
HOURS OF PRODUCTS TO 27 CROSSMATCHED
BLEEDING DEGREES - ↓ COLD
AUTOANTIBODY WRONG
REJUVENATED RBCS IRRADIATION –
– PREPARED WITHIN PLT PREP – 20-24 DISCARD UNIT
3 DAYS DEGREES
- WASHED AND IRRADIATION TWICE
FROZEN AND OPEN SYSTEM – - ↑ POTASSIUM
TRANSFUSED TRANSFUSED
WITHIN 24 WITHIN 24 HRS FFP, CRYOPPT,
HRS FROZEN RBCS – DRY
UNIT WITH CLOT – ICE
FROZEN RBCS – NOT ISSUED
PREPARED WITHIN 6 GRANULOCYTES,
DAYS ITP – winRHO PLTS – RT
- IVIg
IRRADIATION – RBC – 1-10 DEGREES
ANYTIME, MUST BE CRYOPPT:
TRANSFUSED TX: FACTOR 13 SEAL BLOOD BAG –
WITHIN 28 DAYS - HYPOFIBIRNI AVOID
UPON COLLECTION GENEMIA CONTAMINATION IN
- VWD THAWING
APHERESIS – 3.0 X
10^11 ANTI-IgA FROZEN RBCS – 10
- PH > 6.2 ANTIBODIES YEARS
- WASHED CRYOPPT FFP – 1
CGD – RBCS YEAR
GRANULOCYTES PLT CONC – 5 DAYS
TX: GRANULOCYTE FFP TRANSFUSION –
CONC NO REGARD WITH SINGLE DONOR –
RH TYPING LESS PREP
CRYOPPT AFTER - DIFFICULT TO PLTPHERESIS – 24
THAWING FIND DONORS TIMES IN 1 YEAR
- 4 HRS EXPIRY - DO
(POOLED) AUTOLOGOUS TEMP - <99.5
- 6 HRS EXPIRY DONATION DEGREES
IF STERILE
METHOD IS WHOLE BLOOD NAT – HIV, HCV
USED COLLECTION –
DONE WITHIN 15 1ST TEST – REACTIVE
LEUKOREDUCED - MINS 2ND TEST (RERUN) –
<5.0 X 10^6 WBCS NONREATIVE
ACUTE
SUPPRESSED BM – NORMOVOLEMIC - BLOOD
ALL CELL LINES HEMODILUTION – COMPONENTS
SHOULD BE REPLACED WITH MAY BE USED
REPLENISH CRYSTALLOIDS
- UNITS ARE 1ST MARKER TO
MALARIA – 3 YEARS REMOVED APPEAR – HbsAg
- STORED AT RT
NO SYMPTOMS – NO - INFUSED HIV FOR BLOOD
DEFFERAL FOR WITHIN 8 HRS DONOR – ANTI-HIV
DONORS WITH HEP ½
B VACCINE DOUBLE RBC
APHERESIS - > 40% HIV-1 – NAT
PIERCING – 12 HCT
MONTHS RPR – POS
RECENT HBSAG FTA-ABS – NEG
HBIG – I YEAR VACCINE – NO
DEFFERAL WITH OR - BLOOD UNIT
BP - <180 WITHOUT CAN BE USED
SYSTOLIC/<100 SYMPTOMS
DIASTOLIC ANTI-CMV – TESTED
INFLUENZA, IN BLOOD
FORMULA: BLOOD YELLOW FEVER PRODUCTS
COLLECTED/100 X 14 VACCINE – 2 WEEKS - NOT
–X ROUTINELY
RUBELLA – 4 WEEKS TESTED IN BB
63 – X = TOTAL OF
ANTICOAGULANT PLT PHERESIS – 2 WESTERN BLOT – 2/3
TO BE REMOVED DAY INTERVAL POSITIVE
HYPERVENTILATIO AUTOLOGOUS - P24, DP41, GP120, GP

N – STOP DONATION DONATION – 3 DAY 160
INTERVAL
PX WITH ANTI-k –
BEST CANDIDATE
HDNF
ANTI-K – WONT ↑ UNCONJUGATED

CAUSE HDN – DAT BILIRUBIN – BASAL
(-) GANGLIA –
KERNICTERUS
NEGATIVE FETAL
SCREEN – 1 VIAL ↑ TITER OF
RHOGAM ANTIBODIES OTHER
THAN ANTI-D –
CORD CELLS WITH PLASMAPHERESIS
EXCESSIVE
WHARTON’S JELLY – EXCHANGE
WASH 6 TIMES – IF TRANSFUSION –
STILL POSITIVE – ABO, RH, ANTIBODY
OBTAIN HEELSTICK SCREEN AND
PUNCTURE CROSSMATCH
24 WEEKS AB+ BABY WITH
PREGNANT – HDN – TRANSFUSE
(POSITIVE O- OR AB- PRBC
ANTIBODY SCREEN)
– ANTIBODY ID HbS – SHOULD BE
SCREENED FOR
ONLY ANTI-D HDN EXCHANGE
IS REVERSIBLE TRANSFUSION
- CAUSE
FIRST SEMESTER HYPOXIA
MISCARRIAGE –
MINIDOSE
CANDIDATE FOR ANTI=A,B – CROSSES
RHOGAM – O PLACENTA
NEGATIVE MOTHER
GIVING BIRTH TO A
POSITIVE BABY
- NO ANTI-D
YET
NUMBER OF CELLS
CTED/2000 X 5000 = X
X/30 + 1 = NUMBER
OF DOSES

Blood Bank Harr

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Blood Bank Harr

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BLOOD BANK

BLOOD BANK – IgM – BINDS 10 ADSORPTION-

DISCREPANT BLOOD FORWARD BT – POS Ax – DON’T

IMMUNOSUPPRESSI - COLD - AGGLUTINAT

ANTI-H, ANTI-S – COLD

ANTI-Kpa – LOW FREQUENCY AB

ANTI-k – HGH FREQUENCY

SCREENING CELLS – (+) AT IS (RT)

6 UNITS CROSSMATCHED – 5/6

- LOW FREQ ANTIGEN

HYPERVENTILATIO AUTOLOGOUS - P24, DP41, GP120, GP

ANTI-K – WONT ↑ UNCONJUGATED

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