Cambridge IGCSE: BIOLOGY 0610/52
Cambridge IGCSE: BIOLOGY 0610/52
Cambridge IGCSE: BIOLOGY 0610/52
* 2 8 9 9 3 9 1 4 0 7 *
BIOLOGY 0610/52
Paper 5 Practical Test February/March 2024
1 hour 15 minutes
You will need: The materials and apparatus listed in the confidential instructions
INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.
INFORMATION
● The total mark for this paper is 40.
● The number of marks for each question or part question is shown in brackets [ ].
Total
DC (CJ/SG) 328329/4
© UCLES 2024 [Turn over
2
1 You are going to investigate the effect of glucose concentration on the rate of anaerobic respiration
in yeast.
Anaerobic respiration in yeast breaks down glucose to form ethanol and carbon dioxide.
Anaerobic respiration in yeast causes the blue dye, methylene blue, to become colourless. The
time taken for the blue colour to disappear can be used as a measure of the rate of anaerobic
respiration in yeast.
Read all the instructions but DO NOT DO THEM until you have drawn a table for your results
in the space provided in 1(a)(i).
You should use the safety equipment provided while you are doing the practical work.
Step 1 Label one test-tube 0.0%, one test-tube 0.5% and one test-tube 1.0%. Put the test-tubes
in the test-tube rack.
Step 2 Put 5.0 cm3 of water into the test-tube labelled 0.0%.
Step 3 Put 2.5 cm3 of 1.0% glucose solution and 2.5 cm3 of water into the test-tube labelled
0.5%.
Step 4 Put 5.0 cm3 of 1.0% glucose solution into the test-tube labelled 1.0%.
Step 5 Stir the contents of the beaker containing the yeast suspension with the glass rod.
Step 6 Add 5.0 cm3 of the yeast suspension to each of the test-tubes labelled 0.0%, 0.5% and
1.0%.
Step 7 Put all three test-tubes into the beaker labelled water-bath.
Raise your hand when you are ready for hot water to be added to the water-bath.
Step 9 After three minutes, remove the test-tubes from the water-bath and place them in the
test-tube rack.
Step 10 Use a pipette to add one drop of methylene blue dye to each of the test-tubes.
Carefully mix the contents of each test-tube with the glass rod.
Step 11 Use a second pipette to slowly add a layer of oil to each of the test-tubes.
The layer of oil will float on top of the yeast suspension and methylene blue mixture, as
shown in Fig. 1.1.
layer of oil
1.0 cm
to maintain
anaerobic
conditions
test-tube contents
Fig. 1.1
Step 12 Put the test-tubes back into the water-bath and restart the stop-clock.
Step 13 Measure the time taken for the blue colour in each of the test-tubes to disappear.
Record the time taken in seconds for each test-tube in your table in 1(a)(i).
If the colour is still blue after 10 minutes, stop timing and record the time as >600 in your
table in 1(a)(i).
[4]
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [1]
..................................................................................................................................... [1]
(iv) State one variable that was kept constant in this investigation.
..................................................................................................................................... [1]
(v) Explain why it was important to stir the yeast suspension in step 5.
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [1]
(b) One way to improve this investigation would be to use an increased number of different
concentrations of glucose.
1 .........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
2 .........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
[2]
(ii) Describe how you would make 5.0 cm3 of 0.25% glucose solution using a 0.50% glucose
solution and distilled water.
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [2]
(iii) Describe the method you would use to test a solution for the presence of glucose.
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [2]
(c) A student investigated the effect of temperature on the rate of respiration in yeast at 25 °C
and 35 °C.
In one experiment, the student measured the volume of carbon dioxide produced by the yeast
every 5 minutes for a total of 30 minutes.
(i) Describe suitable apparatus that could be used to collect and measure the volume of
carbon dioxide produced.
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [1]
The student did three experiments at each temperature. They used the results to calculate
the mean volume of carbon dioxide produced.
Part of the student’s results table for the experiments at 35 °C is shown in Table 1.1.
Table 1.1
(ii) The student decided that the result of one of the experiments shown in Table 1.1 was
anomalous.
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [1]
(iii) Describe how the student calculated the mean volume of carbon dioxide produced
shown in Table 1.1.
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [1]
The results of the whole investigation about the effect of temperature on the rate of
respiration in yeast at 25 °C and 35 °C are shown in Table 1.2.
Table 1.2
5 0.0 0.1
10 0.0 0.8
15 0.1 1.9
20 0.2 2.7
25 0.5 3.2
30 1.1 3.2
(iv) Using the data in Table 1.2, compare the mean volumes of carbon dioxide produced at
25 °C and 35 °C.
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
..................................................................................................................................... [2]
(v) Plot a line graph on the grid of mean volume of carbon dioxide produced against time,
using all of the data in Table 1.2.
You will need to plot the data for each temperature as separate lines on your graph.
[5]
(vi) Estimate the time taken to produce 3.0 cm3 of carbon dioxide at 35 °C.
.................................................. minutes
[2]
© UCLES 2024 0610/52/F/M/24 [Turn over
8
(d) Carbon dioxide gas was bubbled through hydrogencarbonate indicator solution.
The indicator was red before the gas was bubbled through.
............................................................................................................................................. [1]
[Total: 27]
BLANK PAGE
magnification ×340
Fig. 2.1
[4]
length of PQ .............................................. mm
Calculate the actual length of cell X using the formula and your measurement.
......................................................... mm
[3]
© UCLES 2024 0610/52/F/M/24 [Turn over
12
Plan an investigation to determine the effect of the concentration of sodium chloride solution
on osmosis in plant tissue.
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
...................................................................................................................................................
............................................................................................................................................. [6]
[Total: 13]
Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.
To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge
Assessment International Education Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download
at www.cambridgeinternational.org after the live examination series.
Cambridge Assessment International Education is part of Cambridge Assessment. Cambridge Assessment is the brand name of the University of Cambridge
Local Examinations Syndicate (UCLES), which is a department of the University of Cambridge.