A25 Service Manual - English

A25 - Y25
SERVICE MANUAL
ENGLISH
SERVICE MANUAL
English
TESE-0007-07-ING
May – 2012
TABLE OF CONTENTS
1. INTRODUCTION.................................................................... 7
1.1. GENERAL DESCRIPTION OF THE ANALYZER...............................................................7
1.1.1. Operating arm......................................................................................................................................... 8
1.1.2. Dispensing system................................................................................................................................ 8
1.1.3. Reactions rotor and reading................................................................................................................. 9
1.1.4. Electronic system................................................................................................................................ 10
1.1.5. Application program............................................................................................................................ 10
1.2. FUNCTIONING OF THE ANALYZER...............................................................................11
1.3. TRANSPORT AND RESHIPMENT OF THE ANALYZER................................................11
2. Electronic system and fluids............................................. 13

Description of the electronics of the A25 analyzer.............................................................13
2.1 CPU Board (CIIM00006)...................................................................................................13
2.2 Power Supply Board (CIIM00007)...................................................................................18
2.3 Needle Board (CIIM00008)...............................................................................................20
2.4 Photometry Board (CIIM00009).......................................................................................21
2.5 Racks Board (CIIM00010)................................................................................................22
2.6 LED Board (CIIM00011)....................................................................................................22
2.7 Communications Board (CIIM00019)..............................................................................23
2.8 List of components..........................................................................................................24
2.9 Auxiliar channel information...........................................................................................24
2.10 Boards interconnection . ..............................................................................................29
2.11 Schematic liquid circuit.................................................................................................38
3. SERVICE PROGRAM.......................................................... 39
3.1 Initialising the analyser...................................................................................................39
3.2. ADJUSTMENTS ..............................................................................................................40
3.2.1. Adjustment of the needle thermostatation system........................................................................... 41
3.2.2. Adjustment of the rotor thermostation system................................................................................. 42
3.2.3. Adjustment of the positioning of the operating arm......................................................................... 42
3.2.3.1 Adjustment of X, Y and Z position for reagent and pediatric racks.............................................. 43
3.2.4. Adjustment of the positioning of the rotor........................................................................................ 45
3.2.4.1. Centering of the rotor with regard to the dispensing point.......................................................... 45
3.2.4.2. Centering of the rotor with regard to the optical system.............................................................. 46
3.2.5 . Adjustment of the positioning of the filter wheel............................................................................. 46
3.2.6. Adjustment of the level control scales............................................................................................... 47
3.2.7. Adjustment of the level detection sensitivity.................................................................................... 47
3.3. TESTS...............................................................................................................................48
3.3.1. Motor tests............................................................................................................................................ 48
3.3.1.1. Initialization test................................................................................................................................ 49
3.3.1.2. Movement test................................................................................................................................... 49
3.3.1.3. Loss step test.................................................................................................................................... 50
3.3.1.5. Z axis security systems test............................................................................................................ 50
3.3.1.6 Maximum Z verification test.............................................................................................................. 51
3.3.2. Diaphragm pumps and electrovalves test......................................................................................... 51
3.3.2.1. Functioning test................................................................................................................................ 52
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Service Manual
3.3.2.2. Stress mode test............................................................................................................................... 52

3.3.3. Needle self-centering system test...................................................................................................... 52
3.3.4. Needle level detection system test..................................................................................................... 52
3.3.5. Needle thermostatation system test.................................................................................................. 53
3.3.6. Rotor thermostatation system test..................................................................................................... 54
3.3.7. Photometry tests.................................................................................................................................. 54
3.3.7.1. Base line and integration times....................................................................................................... 55
3.3.7.2. Darkness counts............................................................................................................................... 56
3.3.7.3. Repeatability without moving the filter wheel................................................................................ 57
3.3.7.4. Stability.............................................................................................................................................. 57
3.3.7.5. Repeatability moving filter wheel.................................................................................................... 58
3.3.7.6. Absorbance measurement............................................................................................................... 58
3.3.7.7. Reactions rotor check...................................................................................................................... 59
3.3.8. Level control scales test..................................................................................................................... 59
3.3.10. PC-Analyzer communications channel test.................................................................................... 61
3.3.11. Global stress mode of the analyzer.................................................................................................. 61
3.3.12. Photometry tool.................................................................................................................................. 62
3.4. UTILITIES ........................................................................................................................62
3.4.1. Disassembly of the dispensing needle.............................................................................................. 63
3.4.2. Prime the fluid system......................................................................................................................... 63
3.4.3. Cleaning of the dispensing system.................................................................................................... 64
3.4.4. Changing the lamp............................................................................................................................... 64
3.4.5. Configuration of the filter wheel......................................................................................................... 65
3.4.6. Demonstration mode........................................................................................................................... 66
3.4.7 Read/load adjustments and cycles..................................................................................................... 66
3.4.8 Change the rotor type........................................................................................................................... 67
3.5. REGISTER........................................................................................................................68
3.5.1. Introducing the analyzer serial number............................................................................................. 68
3.5.2. Service Reports.................................................................................................................................... 68
3.5.3. Language change................................................................................................................................. 69
3.5.4. Users..................................................................................................................................................... 69
3.6. MONITOR ........................................................................................................................69
3.7 User’s program . ..............................................................................................................70
3.7.1 Configuration of the level of access to the analyser ........................................................................ 70
3.7.2 Reagent Consumption ......................................................................................................................... 72
4. RECOMMENDATION........................................................... 74
4.1. RECOMMENDED PREVENTIVE MAINTENANCE..........................................................74
4.2. CARE AND CLEANING....................................................................................................74
4.2.1. General care of the analyzer............................................................................................................... 74
4.2.2. Cleaning the optical system................................................................................................................ 75
4.2.3. Cleaning the dispensing system........................................................................................................ 75
4.2.4. General cleaning of the interior of the apparatus............................................................................. 76
A I. TECHNICAL SPECIFICATIONS....................................... 77
A II. ADJUSTMENT MARGINS TABLES................................ 82
A III. LIST OF CONSUMABLES, ACCESSORIES AND SPA-
RES.......................................................................................... 83
A IV. LIST OF REQUIRED TOOLS.......................................... 88
A V. SOFTWARE VERSIONS.................................................. 89
A VI. REFURBISH INSTRUCTIONS........................................ 93
6
1. INTRODUCTION
The A25 analyzer is an automatic random access analyzer specially designed for performing biochemical and turbi-
dimetric clinical analyses. The instrument is controlled on-line in real time from an external dedicated PC.
In each of the components of the A25 analyzer, BioSystems has used leading edge technology to obtain optimum
analytical performance, as well as taking into account economy, robustness, easy use and maintenance. A three-axis
Cartesian operating arm prepares the reactions. Dispensing is performed by means of a pump with a ceramic piston
via a detachable thermostatised needle with Fuzzy Logic control. A washing station guarantees that the needle is
kept perfectly clean throughout the process. The reactions take place in a thermostatised rotor in which absorbance
readings are taken directly by means of an integrated optical system.
This manual contains the information required for learning about, maintaining and repairing the A25 automatic analy-
zer. It should be used by the Technical Service as a learning and consultation document for the maintenance and
repair of the instrument. Chapter 2 describes the different mechanical components that form the analyzer together
with their functionality, and chapter 3 describes the electronic system. Chapter 4 describes the Service Program.
All the adjustments and checks of the analyzer are carried out through this program, which is independent from the
application program (User Program). The separation of both programs enable it to be maintained separately and the
extensions and improvements of one do not affect the other. The user does not have the service program. The Te-
chnical Service must install it on the user’s computer in order to carry out the service requirements. Once said tasks
have been carried out, the Technical Service must uninstall the program. Chapter 5 offers instructions for the different
maintenance, repair and cleaning operations that can be carried out by the Technical Service. The annexes contain
a summary of the technical specifications of the analyzer, the adjustment margin tables, the lists of accessories and
spares, a list of software versions and their compatibility and a software troubleshooting guide.
1.1. GENERAL DESCRIPTION OF THE ANALYZER
The A25 analyzer is made up of three basic components: the operating arm, the dispensing system and the reading
and reactions rotor. The electronic system of the instrument controls said components and communicates with the
external computer containing the application program. Through this program, the user can control all the operations
of the analyzer. The analyzer may be fitted with the option of an external module for measuring ion concentration.
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Service manual
1.1.1. Operating arm
This is a three-axis XYZ Cartesian mechanism. The X and Y axes move the dispensing needle over the analyzer
horizontally and the Z axis moves it vertically. It is operated by three step-by-step motors. In each 15-second prepara-
tion cycle, the operating arm performs the following actions: first of all, it sucks in the reagent from the corresponding
bottle. Next, the needle is washed externally in the washing station and sucks in the sample from the corresponding
tube. It is washed externally again and dispenses the sample and the reagent into the reactions rotor. Finally, it is
exhaustively washed internally and externally before proceeding with the next preparation. The arm has a system
for controlling vertical movement to detect whether or not the needle has collided into anything on descending. If a
collision occurs, as may be the case if, for example, a lid has been left on a bottle of reagent, the arm automatically
restarts, verifies the straightness of the needle and continues working issuing the corresponding alert to the user.
A vertical axis retention system prevents the needle from falling in the case of a power cut, avoiding injury from the
needle to the user or the needle being bent by an attempt to move the arm manually. The operating arm only makes
the preparations if the general cover of the analyzer is closed. If the cover is raised while it is functioning, the arm
automatically aborts the task in progress and returns to its parked position to avoid injury to the user.
1.1.2. Dispensing system
This system consists of a thermostatised needle, supported and displaced by an operating arm and connected to a
dispensing pump. The needle is detachable to enable cleaning and replacement. The analyzer has capacity level
detection to control the level of the bottles and tubes and prevent the needle from penetrating too far into the corres-
ponding liquids, thus minimising contamination. An automatic adjustment system informs the user if the needle is
not mounted or if it is too bent. The needle has a sophisticated Peltier thermostatisation system, with Fuzzy Logic
control, capable of thermostatising the preparations at approximately 37º in less than 6 seconds. Dispensing is carried
out by means of a low maintenance ceramic piston pump driven by a step-by-step motor. It is capable of dispensing
between 3 and 1250 µL. The exterior of the needle is kept constantly clean by means of a washing station, which
consists of a font specially designed to clean and dry the needle, integrated in the racks tray. A system of diaphragm
pumps supplies the font with distilled water and transports the waste to its container.
The A25 analyzer has a tray with 3 free positions for racks of reagents or samples, plus three fixed positions for bottles
opposite the washing station. Each reagents rack can carry up to 10 reagents in 20 ml or 50 ml bottles. Each sam-
ples rack can contain up to 24 tubes of samples. The samples can be patients, calibrators or controls. The analyzer
can be configured to work with 13 mm or 15 mm diameter tubes of samples with a length of up to 100 mm or with
paediatric wells.
Any possible configuration of racks can be mounted from 3 racks of samples (72 samples) and 30 refrigerated bottles
of reagent to 2 racks of unrefrigerated reagents (20 reagents) and 30 refrigerated bottles of reagent and 1 rack of
samples (24 samples). Any reagent may be placed in the fixed positions, but it is recommendable to use them for the
bottles of distilled water, saline solution for the automatic pre-dilutions and washing solution. The rack tray detects and
8
identifies the type of racks. In this way, if the physical disposition of the racks does not coincide with that programmed
on the comp uter, the analyser alerts the user.
On the left of the analyser are system liquid containers (marked in blue) and waste containers (marked in red), and
on the back of the analyser is connected the bottle of washing solution (marked in green). The analyser constantly
controls the level of these containers and issues the appropriate alerts if the system liquid or washing solution is nearly
empty or if the waste container is full.
1.1.3. Reactions rotor and reading
The preparations are dispensed in an optical quality methacrylate reactions rotor thermostatised at 37ºC. The optical
absorbance readings are taken directly on this rotor. Each reaction can be read for 15 minutes. The readings are taken
as they are programd in each measurement procedure. The reaction wells have been designed to enable the mixture
of the sample and the reagent during the dispensing. Each rotor has 120 reaction wells. The length of the light path is
6 mm. The minimum volume required to take the optical reading is 200 µL. The wells have a maximum useful capa-
city of 800 µL. When the reactions rotor is completely full, the user must change it with one that is empty, clean and
dry. The reactions rotors can be reused up to 5 times if they are carefully cleaned immediately after use. The rotor is
driven by a step-by-step motor with a transmission. A Peltier system with PID control thermostatises the rotor at 37ºC.
An optical system integrated in the rotor takes the readings directly on the reaction wells. The light source is a 20 W
halogen lamp. The detector is a silicon photodiode. The wavelength is selected by a wheel with 9 positions available
for optic filters. The filters are easily changed by the user from the exterior of the analyzer, without the need for disas-
sembling the filter wheel. A step-by-step motor positions the wheel. The optical system is capable of taking 5 readings
per second, with or without a filter change in between. The light beam of the lamp passes through a compensated
interferential filter to select the desired wavelength and through focalisation lenses. It then passes through the rotor well
and finally reaches the photodiode, where the light signal is turned into an electric signal. A sophisticated analogical
digital integrator-converter system converts the electric signal into a digital value with which the analyzer obtains the
absorbance values. The optical system continues to work when the general cover of the analyzer is open, whereby the
analyzer can continue to take readings while the user handles, for example, the sample tubes or the reagent bottles.
The rotor cover must be in placefor the optical system to work correctly. A detector tells the analyzer of the presence
of the cover. The analyzer aborts the readings if the user removes the rotor cover while the optical system is taking
photometric measurements. If the rotor is not covered, the analyzer informs the user so that he or she places the rotor
cover when it sends samples to be analyzed.
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Service manual
1.1.4. Electronic system
The described components are controlled by an electronic system based on a microprocessor. The microprocessor
has two external communication channels that make it possible to link up the instrument to the computer containing
the application program and to an optional external module for measuring ion concentration. The electronic system
is made up of the following independent boards:
- Microprocessor board
- Photometric system board
- Needle conditioning board
- Racks detection board
- Front indicator board
- Power supply board
- Communications board
1.1.5. Application program
The application program makes it possible to control all the operations of the analyzer. From this program, the user
can monitor the state of the analyzer and the work session, program parameters, e.g. technique parameters, prepare
the work session, prepare results reports, configure different analyzer options, activate various test utilities, prepare
and maintain the instrument and carry out internal quality control processes. The purpose of this manual is not to
explain the functioning of the user program. For detailed information to this regard, please consult the User’s Manual
included with the analyzer.
1.2. FUNCTIONING OF THE ANALYZER

The A25 analyzer is an automatic random access analyzer specially designed for performing biochemical and turbi-
dimetric clinical analyses. The analyzer performs patient-by-patient analyses and enables the continual introduction
of samples. The analyzer is controlled from a dedicated PC that is permanently communicated to the instrument. The
program, installed on the computer, keeps the user constantly informed of the status of the analyzer and the progress
of the analyses. As results are obtained, the computer shows them to the user immediately.
10
When a Work Session is begun, the analyzer proposes performing the blanks, calibrators and controls programd for
the measurement procedures it is to carry out. The user may choose between performing the blanks and the calibra
tors or not. If they are not performed, the analyzer uses the last available memorised data. The controls can also be
activated or not. During a session, while the analyzer is working, the user can introduce new normal or urgent samples
to be analyzed. Each time a new sample is added, the analyzer automatically proposes the possible new blanks, cali-
brators or controls to be performed. A work session can remain open for one or more days. When a session is closed
and another new session is opened (Reset Session), the analyzer again proposes performing the blanks, calibrators
and controls. It is recommended that the session is reset each working day.
The analyzer determines the concentrations of the analytes based on optical absorbance measurements. To measure
the concentration of a certain analyte in a sample, the analyzer uses a pipette to take a specific volume of the sample
and the corresponding reagent, quickly thermostatises them in the needle itself and dispenses them into the reactions
rotor. The very dispensing speed together with the geometry of the reaction well causes the mixture to be shaken and
the chemical reaction begins. In the bireagent modes, the reaction begins when the analyzer later dispenses a second
reagent in the same reaction well. The reactions can be biochemical or turbidimetric. In both cases, the reaction or
the chain of reactions produced generate substances that attenuate certain wavelengths, either by absorption or by
dispersion. Comparing the light intensity of a certain wavelength that crosses a well when there is a reaction and when
there is not a reaction can determine the concentration of the corresponding analyte. This comparison is quantified with
the physical magnitude called absorbance. In some cases, the concentration is a direct function of the absorbance,
and in other cases, it is a function of the variation of the absorbance over time, depending on the analysis mode.
1.3. TRANSPORT AND RESHIPMENT OF THE ANALYZER
If the analyzer is to be reshipped or moved using a transport vehicle, it is important to block the operating arm and
use the original packaging to ensure that the apparatus is not damaged. To package the instrument, we recommend
you follow the following instructions: (on the unpackaging instructions sheet).
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Service manual
RANDOM ACCESS ANALYZER A25

UNPACKING INSTRUCTIONS
4
Unblock the operating arm taking
out the screws (1), (2), (3) and the
l-braket (4).
To free the lid and it can open and

close freely, cut the tube (5) that
subject it.
If it becomes necessary to return the analyzer, or transport it by vehicle, it is important

to block the operating arm and use the original packaging to avoid any damage to the
instrument.
12
2. ELECTRONIC SYSTEM AND FLUIDS
1. CPU Board (CIIM00006)
2. Power Supply Board (SP300 & CIIM00007)
3. Needle Board (CIIM00008)
4. Photometry Board (CIIM00009)
5. Racks Board (CIIM00010)
6. LED Board (CIIM00011)
7. Communications Board (CIIM00019)
8. List of components
9. Auxiliar channel information
10. Interconnection between boards
11. Schematic liquid circuit
Description of the electronics of the A25 analyzer.
The electronics of the analyzer are made up of different boards located at different points in the analyzer and dedicated
to specific functions. Their different locations correspond to functionality and performance criteria for the functioning
of the analyzer.
There are 8 different boards, which correspond to:
CPU Board (CIIM00006)

Power Supply Board (SP300 & CIIM00007)
Needle Board (CIIM00008)
Photometry Board (CIIM00009)
Racks Board (CIIM00010)
LED Board (CIIM00011)
Communications Board (CIIM00019)
2.1 CPU Board (CIIM00006)
This is the brain of the machine, containing the microprocessor (H8/3003), responsible for controlling all the components
of the machine. The board has different data storage systems using either static RAM (U21), FLASH memory (U18) or
EPROM (U20). The slot associated with the EPROM is used to check the functionality of the board and the recording
of the MONITOR program in the production phases of the analyzer. The other two memories are associated with the
normal functioning of the analyzer. The FLASH memory holds the application itself as well as different databases
related to factory settings, adjustments, state of the rotor and possible extensions to the application.
The U34 device also exists on the board. This is a logical programmable device (FPGA) dedicated to the control of
motors, mapped in register memory associated with end-of-run control, electrovalves, decoding of racks (CIIM00010),
level sensing and control of the photometry-associate board (CIIM00009).
The control of the motors acts directly with the L6208 driver. There are one driver for each motor. The regulation of
the current of each axis can be configured by means of a DAC that sets the current set point independently (U5). The
sequencers are supplied through the U17 regulator and the drivers take their supply through the J16 connector, which
corresponds to the 36V input on the CIIM00006 board.
The action on the thermostatation systems of the analyzer (needle and rotor) is carried out through H-shaped bridges
based on MOS technology (U29 and U30) and controlled directly from the microprocessor. The heating elements are
connected to J27 (needle) and J28 (rotor), respectively.
13
Service manual
Connector Function Pins

J1 Motor Z 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J2 Motor Y 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J4 Motor X shaft 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J5 Pump Motor 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J6 Filter Drum Motor 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J7 Rotor Motor 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J8 Diaphragm inlet Pump 1 - motor
2 - motor
J11 Diaphagm outlet pump 1 - motor
2 - motor
J9 3-way electrovalve 1 - coil 1
2 - coil 1
2 - coil 1
2 - coil 1
2 - coil 1
J15 The signal enabling level detection by the analyzer
needle is injected into the racks tray through this con- 1- Faston
nector.
J16 36V voltage 1 - 36V
2 - GND
J17 12V voltage 1 - GND
2 - 12 V analogical
3 - GND
4 - 12 V peltier
5 - GND
6 - 12 V valves
14
J18 5V voltage 1 - Vcc
2 - GND
3 - Enable
J19 Weighting system for waste and system liquid bottles 1 - Vdc
2 - GND
3 - V+
4 - V-
5 - Vdc
6 - GND
7 - V+
8 - V-
J24 Rotor connector 1 - Vdc
2 - Rotor cover
3 - Home rotor
4 - GND
5 - Vdc home
rotor
6 - Filter wheel
home
7 - GND
8 - Vdc Filter
wheel home
9 - Rotor ther-
mostatation
10 - GND
11 - Analogic 12
V
12 - GND
J25 Communications Board Connection (CIIM00019) 1 - Vdc
2 - GND
3 - Tx0
4 - GND
5 - Rx0
6 - GND
7 - GND
8 - Tx1
9 - GND
10 - Rx1
J27 Needle Peltier 1 - peltier, red
2 - peltier, black
J28 Rotor Peltier 1 - peltier, red
2 - peltier, black
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Service manual

J29 Needle Board Interconnection (CIIM00008) 1 - GND
2 - Home Z
3 - Needle ther-
mistor
4 - Needle level
detector
5 - GND
6 - 12 V
J30 Z axis encoder 1 - Encoder detec-
tor
2 - GND
3 - Vdc
J31 X axis home 1 - Home
2 - GND
3 - Vdc
J32 Y axis home 1 - Home

2 - GND
3 - Vdc
J33 Ceramic pump home 1 - Home
2 - GND
3 - Vdc
J34 Rack decoding board connection (CIIM00010) 1 - Detector 1
2 - Detector 2
.
.
24 - Detector 24
25 - GND
26 - Vdc
J35 Photometric board connection (CIIM00009) 1 - 12 V
2 - GND
3 - DVALID
4 - DCLK
5 - DOUT
6 - DXMIT
7 - RANGE2
8 - RANGE1
9 - RANGE0
10 - TEST
11 - CONV
12 - GND
13 - CLKAD
14 - GND
15 - GND
16 - Vdc
J36 LED board connection (CIIM000011) 1 - GND
2 - LED
3 - LED
4 - Vdc
J99 Fan 1 - 12V
2 - GND
16
J39 Connection of I2C bus to the racks board board 1- 5V
(CIIM00010) 2- SDA
3- SCK
4- GND
J40 Connection of I2C bus to the power supply board 1 - 5V
(CIIM0007) 2 - SDA
3 - CLK
4 - GND
J41 Connection to the photodetectors of probe detection. 1 - 5V
2 - GND
3 - White
4 - Green
5 - 5V
6 - GND
7 - White
8 - Green
J44 External bottle level sensor
1 - Red
2 - Black/yellow
As for analogical circuitry on the board, the J19 connector corresponds to the input of the sensors for machine water
and waste control. These sensors are load cells and they are conditioned by U16 and associated components. The
sensor signal is linearised and amplified and is then redirected to the analogical-digital converters in the microprocessor.
There is also a circuit for conditioning the signal of the thermistor associated to the thermostatation of the rotor that
is made up of the U22 and U28 circuits. The signal used to detect the level is generated by U28. The injection of the
signal is done by J15 and it is collected through the cables that come from the analyzer needle and connect on J29.
There are also different integrated circuits for the encoding of the control signals for all the components that share the
data and address bus (U26, U23, U24 and U36), control signals inversion (U35).
The board has an acoustic alarm.
TP1 - VRAM
TP3 - LOW-LINE
TP4 - RESET
TP6 - WATCHDOG
TP12 - Needle level detector
TP13 - LSO
TP14 - RW
TP20 - CS_EPROM_L
TP21 - CS_RAM_L
TP22 - CSF2_L
TP23 - CSF1_L
TP24 - RD_L
TP25 - CLK system (16 MHz)
TP28 - LWR_L
TP29 - HWR_L
TP30 - Motor chopper frequency
TP36 - Conditioned signal output from the rotor thermistor
TP37 - Analyzer cover
TP39 - Rotor cover
TP40 - CS_FPGA_L
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Service manual
TP44 - INIT_L
TP45 - DONE
TP46 - PROGRAM_L
TP47 - CCLK
TP48 - DIN
TP50 - Z shaft motor vref
TP51 - Y shaft motor vref
TP52 - X shaft motor vref
TP53 - Dispensation/aspiration pump motor vref
TP54 - Filter drum motor vref
TP55 - Rotor motor vref
TP56 - IN1 Needle Peltier Driver
TP57 - EF Needle Peltier Driver
TP58 - IN2 Needle Peltier Driver
TP59 - IN1 Rotor Peltier Driver
TP60 - EF Rotor Peltier Driver
TP61 - IN2 Rotor Peltier Driver
The replacement of the CPU (PC15061) can be mounted in the A25 analyzer without fridge and in the analyzer A25
with a fridge.
When installing a CPU board (PC15061) in a non A25 fridge analyzer, connect the cables as follows:
Item Cables PC15061 board

connector
Diaphragm pumps PLMA0047 J8
EV 2 way PLMA0019 J13
EV 3 way PLMA0022 J12
The connectors J9, J10 and J11 are unconnected.
2.2 Power Supply Board (CIIM00007)
This is made up of 5 different switched voltage regulators that enable distribution of the power supply in accord with
the requirement of each subsystem.
J1 5 V output and enabling of power 1 - 5V

supply board 2 - GND
3 - ENABLE
J2 Output voltage 12 V for lamp supply 1 - 12 V
2 - GND
J3 36 V input from the SP300-36 power 1 - 36V
supply board 2 - NC
3 - GND
J5, J6 Fan connection 1- 12V
2 - GND
J8 Connection to I2C bus with the CPU 1- 5V

board (CIIM0006) 2- SDA
3- SCK
4- GND
18
J4 36V and 12 V output voltage. Su- 1 - 36V

pplies voltage for motors, electro- 2 - GND
valves and membrane pumps and 3 - 12V Valves
thermostatisation systems. 4 - GND
5 - 12 V thermostati-
sation
6 - GND
7 - 12 V analogic
8 - GND
J5,J6 Fan connection on the central back 1 - 12V
cover. 2- GND
J8 Connection to I2C bus to connect with 1- 5V

the cpu board 2- SDA
3- SCK
4- GND
TP36V - 36V voltage

TPGND - GND
TP1 - 5V digitals
TP2 - Lamp voltage from 11.7 V <12V
TP3 - 12V Analogicals
TP4 - 12V Valves and diaphragm pumps
TP5 - 12V thermostatation
The existing bridge is to enable the different supply voltages. It has the same function as the enable of the J1 connector.
List of LED diodes:
D3 - Indicates 5V activated
D4 - Indicates 12V lamp activated
D7 - Indicates 12V valves activated
D8 - Indicates 12V analogicals activated
D10 - Indicates 12V thermos activated
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Service manual
2.3 Needle Board (CIIM00008)

This board conditions the thermistor signal associated with the thermostatation of the needle, the preamplification of
the level detection signal and the Z home. It receives, from the needle-fan unit associated with thermostatation, the
Peltier cell, the thermistor and the level signal detected by the needle itself.
Two sets of cables leave this needle and join this board with the CIIM00006-01 board (specifically with the J29 and
J27 connectors) for its J1 and J2 connectors.
J1 CPU board connection (CIIM00006) 1 - Fan control

2 - Home Z
3 - Thermistor
4 - Sensor level
5 - GND
6 - 12V
J2 CPU board connection (CIIM00006) for the Peltier 1 - Peltier

2 - Peltier
3 - Chassis
J3 Thermistor 1 - GND
2 - Thermistor
J4 Peltier 1 - Peltier, red

2 - Peltier, black
J5 Fan 1 - Fan control

2 - 12V
J6 Needle connection for level detection 1 - needle
TP1 - Needle signal

TP2 - Output preamplifier needle signal
TP3 - Output amplifier thermistor signal
TP4 - Thermistor
12V - 12V voltage
20
5V - 5V voltage
AGND - GND
2.4 Photometry Board (CIIM00009)

This board also has the heart of the absorbance measuring system for the samples to be analyzed. It is made up of
a photodetector and an associated analogical-digital conversion circuitry (DDC112).
JP1 - soldering bridge - Solder only if the local oscillator and the U4 and U5 flip-flop, respectively, are not present.
JP2 - soldering bridge - The same than JP1
JP3 - soldering bridge - joins together the analogical and digital ground
J3 Photometric board connection (CIIM00009) 1 - 12 V

2 - GND
3 - DVALID
4 - DCLK
5 - DOUT
6 - DXMIT
7 - RANGE2
8 - RANGE1
9 - RANGE0
10 - TEST
11 - CONV
12 - GND
13 - CLKAD
14 - GND
15 -GND
16 - Vdc
21
Service manual
2.5 Racks Board (CIIM00010)

This board decodes the rack type the analyzer has at each of the positions for said racks. It is made up of a battery
of photobarriers that allow or stop light passing through the rack identification tabs at logical levels the analyzer fir-
mware can handle.

J1 CPU(CIIM0006)board con- 1- Detector 1
nection 2- Detector 2
...
...
24- Detector 24
25- GND
26- 5V
J2 Fridge LED connection 1 -5V
2- GND
J3 Temperature sensor fridge 1- Sensor
connection 2- Sensor
J4 Board CPU I2C connection 1- 5V
2- SDA
3- SCK
4- GND
J5 Fridge detection signal faston 1- Sensor
J7 Cover fridge detection sensor 1- Sensor
2- Sensor
J9 Fridge peltier 1- Peltier red
2- Peltier black
2- Peltier black
2- Peltier black
2.6 LED Board (CIIM00011)
This board indicates the different states of the analyzer. It is made up of a bicolor LED and the circuitry associated
with action.
D1 - Bicolor LED
J36 LED board connection (CIIM000011) 1 - GND

2 - LED
3 - LED
4 - Vdc
22
2.7 Communications Board (CIIM00019)
This enables communication with the exterior of the analyzer through a USB channel or a RS232 channel. It also
includes an auxiliary RS232 channel for monitoring the functions of the analyzer during its execution.
CN1 - USB Connector

P1 - Main RS232 connector
P2 - Auxiliary RS232 connector
D1 - USB TX LED indicator

D2 - USB Rx LED indicator
J1 CPU board connection (CIIM0006) 1- Vcc

2- GND
3- TX0
4- GND
5- RX0
6- GND
7- GND
8- TX1
9- GND
10- RX1
23
Service manual
2.8 List of components
Component Reference
2 way electrovalve SIRAYD 10302.Z031C 12VCC
3 way electrovalve LVM115-6A-2U-1 from SMC
Cell load DF2SR-3 20K
Cover magnet Neodimio D4x5
Electronic box fan SUNON KDE1208PTV113.MS.A.GN
Filter wheel motor NMB23ML-C343V-1
Hall efect sensor RELE REED A041 1D 2H0500
Home detector TCST1300
Lamp 12V 20WG4 BLV
Membrane pump MRL12VDC
Probe fan NMB2404 KL-04W-B40
Probe temperature sensor 2.2K3A1iAM
Pump motor NMB23ML-C343V-1
Rotor fan SUNON KDE1208PTV113.MS.A.GN
Rotor motor NMB23ML-C343V-1
Rotor peltier CP1.0-127-05L-1-EP
Rotor temperature sensor 2.2K3A1iAM
X motor HY-200-2226-0160-AX08
Y motor NMB23ML-C343V-1
Z motor NMB17PMKD18V
2.9 Auxiliar channel information
The rear left part of the instrument is where the communications cables are connected. There are two connections,
the COM1 and the COM2.
The COM1 is the main connection from the analyser to the computer. This connection should be always present to
analyser run propertly.
there are two connection types:

A - Cable type USB
B - Cable type RS-232
Only connect one cable type.
The labeled connector COM2 is the auxiliar connector.

This connector is used to communicate with a second serial port in the computer. The function of this cable is to mo-
nitor the internal states of the analyser.
To show all this information, the user should execute the program: windows HyperTerminal and configure with the
following parameters:
Baud Rate: 38400

Número de bits: 8
Stop bits: 1
Paridad: none
24
Onces is configured and connected the cable, switch on the analyser. In this moment will appear in the HyperTerminal
screen information about the analysers mode and the different executes states. In the initializate mode, the analyser
do an internal checking for each element, if someone has any error then in the screen will show the element that fails.
The following lines shows an exemple of the instructions during an initialization, (this information could change with
the improvements of the firmware) :
BioSystems S.A. 2007

Monitor Firmware A25 v3.0
- Forced Monitor?: No
- Monitor Mode?: No
- Checking User Firmware: Found Firmware
Checksum Ok
- Launching User Firmware!
[A25V2]
BIOSYSTEMS A25 [FRIDGE]
Firmware initialization
Firmware Version: A25 User V3.30
Serial Number: 831013000
FLASH functions transferred to RAM

Interrupt Vectors transferred to RAM
Interrupts enabled
Checking firmware integrity

Checking program checksum:
Checksum correct! Program Checksum=0xA585 Size=450664
Checking A25 configuration checksum:

Checksum correct! Configuration Checksum=0xE04E Size=932
Checking A25 configuration backup checksum:

Checksum correct! Configuration backup Checksum=0xE04E Size=932
Loading A25 Configuration from FLASH
25
Service manual
Configuration in FLASH is correct
Adjustments loaded:
Analyzer S/N: 831013000

Extended S/N: 00000000000000000000
Temperature correction for Rotor=-0.31
Temperature correction for Probe=-0.64
System Liquid Low Level=432 (0%)
System Liquid High Level=648 (100%)
Waste Low Level=576 (0%)
Waste High Level=844 (100%)
Sensitivity of level detection:
- Tube 13/15mm=112
- Pediatric=101
- Reagent=46
- Fridge Reagent=46
Origin X=14
Origin Y=29
Origin Z=465
Tray Reference X=1345
Tray Reference Y=344
Washing station X=1347
Washing station Y=23
Washing station Z=275
Fridge Reference X=1721
Fridge Reference Y=1316
Fridge Reference Z=0
Reactions Rotor X=2873
Reactions Rotor Y=679
Reactions Rotor Z=415
Rotor Distance between the dispensation point and the optic system=1427
Rotor Position correction regard to the dispensation point=-2
Rotor Position correction regard to the optic system=-7
Filters Wheel correction=-4
Filters and their Integration Times:
Filter 1=000 Integration Time= 10ms ( 98) Reference Time= 10ms ( 97)
Filter 2=340 Integration Time= 70ms (684) Reference Time= 70ms (683)
Filter 10=000 Integration Time= 10ms ( 98) Reference Time= 10ms ( 97)
TI/LB Hystoric:
F[01]: 00004018 00004046 00004064 00004008 00004000 00004051 00004042 00004111 00004060 00004029
F[02]: 00616499 00615007 00622104 00618388 00610488 00608066 00613470 00597748 00601531 00609393
F[03]: 00897499 00898168 00898858 00897966 00895314 00896380 00899938 00896324 00897692 00896351
F[04]: 00895331 00894947 00895349 00894860 00898594 00897303 00895865 00897859 00896842 00898261
F[05]: 00898856 00898348 00898632 00898263 00897672 00895180 00899704 00896637 00894443 00897725
F[06]: 00897651 00896160 00898222 00897234 00899144 00895317 00896807 00895461 00899144 00896267
F[07]: 00899631 00898217 00897954 00897230 00897751 00899972 00896751 00896357 00898677 00898857
F[08]: 00896040 00899758 00897740 00896050 00897510 00899633 00897053 00897614 00899586 00898564
F[09]: 00897553 00897843 00897142 00899308 00898354 00899756 00896360 00899912 00899935 00897069
F[10]: 00003993 00004052 00004066 00004014 00004033 00004010 00004017 00004052 00004023 00004029
F[01]: 098 098 098 098 098 098 098 098 098 098
F[02]: 684 684 684 684 684 684 684 684 684 684
F[03]: 177 178 176 177 179 180 179 183 182 178
F[04]: 161 162 160 161 165 165 163 167 166 163
F[05]: 152 153 151 152 155 155 154 157 156 153
F[06]: 178 179 177 178 182 182 180 184 184 179
F[07]: 180 181 179 180 181 183 181 185 185 179
F[08]: 193 195 192 193 196 198 195 200 200 193
F[09]: 229 231 228 230 232 234 231 237 237 228
F[10]: 098 098 098 098 098 098 098 098 098 098
Zmax Reference=170
- Pediatric Offset=-276
- 13mm Offset=1
26
- 15mm Offset=0
- Reagent Offset=4
- Central Reagent Offset=0
- Fridge Reagent Offset=16
A25 Mechanical History
- X axis: 3247 Steps

- Y axis: 1350 Steps
- Z axis: 2249 Steps
- Rotor: 307 Steps
- Filter Wheel: 7141 Steps
- Ceramic Pump: 6971 Steps
- Washing Station Pump: 3630 Cycles
- Washing Station Valve: 3631 Cycles
- Ceramic Pump Valve: 4477 Cycles
- Ceramic Pump Valve Ext1: 889 Cycles
- Ceramic Pump Valve Ext2: 230 Cycles
- Washing Station Pump 2: 3629 Cycles
- Lamp: 498 Minutes
A25 Statistics
- Biochemistry Tests: 208

- Turbidimetry Tests: 77
- Biochemistry Bireagent Tests: 6
- Turbidimetry Bireagent Tests: 39
- Predilutions: 0
- Initial/Final Washings: 115
- Washing Solution Washings: 0
- System Liquid Washings: 82
- New Rotor: 5
- Bireagent Contaminations Solved: 1
Detecting Main Board version (CIIM00006-XX)

... Main Board version will be detected at Warming Up
Setting racks layout
Tray Ref. X=1345 => Distance from tray reference to tray corner X=1082
Tray Ref. Y=344 => Distance from tray reference to tray corner Y=432
Absolute position of tray corner X=263

Absolute position of tray corner Y=-88
Generating Zmax Map:Ok
CPU settings: MDCR=c4;ABWCR=0;ASTCR=ff
BioSystems A25
Hello World
A25 MAGIC KEYS
H: Help
R: Rotor Temperature
P: Probe Temperature
S: Level Scales
A: Last A25 Stress Results
L: Actual Sensitivity of Level Detection
N: Enable Level Detection Debug
Buzzer Control
B: Buzzer On
b: Buzzer Off
Encoder
Y: A15 Encoder profile (Press while booting)
Rotor Reading
1: Choose Filter +
2: Choose Filter -
9: Start Rotor Readings
Notes: Use only in Service Mode
after a Base Line Test.
27
Service manual
Rotor Read
1: Choose Filter +
2: Choose Filter -
Analyzer Memory Dump
G: Memory Dump
Notes: Use only after a Worklist in
Stand By.
This tests dumps all the preparations
parameters received and the photometric
readings.
DDC112/Photometry
D: Choose Mode
- DDC112 internal test mode
- DDC112 Photometry Mode
- Stop
+: Integration Time +0.5ms
-: Integration Time -0.5ms
Notes: Only works in Service Mode
This tests performs continuous
readings with the DDC112.
Remember stop the test for
continue working.
A25 Auto-Check
J: Last results
Caution: Dont’t abuse of this functions while the
analizer is running.
System Monitor
O: Power Supply Monitor
I: Reagent Refrigerator Monitor
W: EV&PUMP Diagnostic
[A25V2]
There are a few keys that work with the Hyperterminal, to press some keys the analyser give information about some
element, the following keys has the function:
H: Help, help, shows the help text

R: Rotor Temperature, shows the rotor temperature
P: Probe Temperature, shows the needle temperatare
S: Level Scales, shows the scales mesures in %
A: Last A25 Stress Results
L: Actual Sensibility of Level Detection
N: Activate additional information of level detection (only internal use)
K: Deactivate the power supply
B: Activate the buzzer
b: Deactivate the buzzer
E: Generate an encoder error (only internal use)
I: Activate the encoder interrupt (only internal use)
1: Increase the filter wheel position
2: Decrease the filter wheel position
9: Mesure the whole rotor, step by step
G: Once finish a work list, push the G and send to the hyperteminal more detailed information of the work list mana-
gement
D: Show the mesure depending on the number of key pressed
1st press: activate the internal test DDC112, always show the same count number
2nd press: activate the normal mesure DDC112, show the count number mesured
3th press: stop the DDC112 mesure
+: Increase the integration time in 0.5ms
-: Decrease the intergration tie in 0.5ms
J- Shows the last result of the list
O- Shows the extra information of the power supply voltatge
I – Shows the information about the state, cover and temperature of the fridge
W- Shows the information about the state of the electrovalve and membrane pumps.
28
FILTRO DE RED
FUSIBLE LINE FILTER
INTERRUPTOR
FUSE FC-3Z
SWITCH
2A (F)
PLMA00159 PLMA00033 PLMA00183
1/2
Negro/Black L
Marron/Brown
Negro/Black
Negro/Black
Negro/Black Azul/Blue N
PLMA00159 PLMA00033
115-240V
Negro/Black
ENTRADA UNIVERSAL
2.10 Boards interconnection
UNIVERSAL INPUT
PLMA00105
Amarillo-Verde/Yellow-Green
Negro/Black
Power-In Scheme
PLMA00157
Marron/Brown
Azul/Blue
29
N
Esquema de Alimentación
Negro/Black
Negro/Black
PLMA00204 FUENTE DE
PLMA00034
PLMA00034
12V ALIMENTACION
RACKS BOARD Rojo/Red
POWER SUPPLY
12
CIIM00010 J6
Negro/Black
GND HWS100-12/A
L
FG
PLMA00158
Amarillo-Verde/Yellow-Green
PLMA00107 N
36V FUENTE DE
Rojo/Red
DC/DC BOARD ALIMENTACION
13
J3
Negro/Black
CIIM00007 GND POWER SUPPLY
SW300-36
L
The following schematics shows the connection between boards and the differents elements has the analyser.
Service manual
Esquema de Alimentación
Power-In Scheme
2/2 FAN 1
FAN 2
LED BOARD
CIIM00011
Negro/Black
Rojo/Red
Naranja/Orange
1 2 3 4
1 2 3 4
PLMA00031
Blanco/White
J36
Verde/Green
J1
1 2
CPU BOARD CIIM00006

J6
Gris/Grey
PLMA00042
(E)
(C)
Negro/Black Rojo/Red
(K) (B)
1 2
1 2 3
1 2 3
J31
J5
Rojo/red Negro/Black
(A)
Amarillo/Yellow
DC/DC BOARD CIIM00007
PLMA00031
PLMA00038
Azul/Blue
1 2 3 4
1 2 3 4
naranja/Orange
J40
I2C
Negro/Black
A25 COVER DETECTOR
J8
Verde/Green
PLMA00215
Azul/Blue
1 2 3
1 2 3
Negro/Black
J1
J18
CPU
V5
Rojo/Red
PLMA00173
1 2
MOT
J16
V36
Azul/Blue
Negro/Black
TERM BM&V
87654321
V12
Verde/Green
1 2 3 4 5 6
Negro/Black
J4
Marron/Brown
V12
J17
Negro/Black
Naranja/Orange
V12
AN
Negro/Black
PLMA00172
30
Esquema de Brazo Manipulador
AUTOCENTER
Operating Arm Scheme
CORNER
(C)
(E)
TEMPERATURE
Verde/Green Verde/Green
(K) (B)
Blanco/White Blanco/White
12345678
(A)
Negro/Black Negro/Black
SENSOR
PROBE Rojo/Red Rojo/Red
J41
Verde/Green Verde/Green
(C)
(E)
(K) (B)
Rojo/Red Rojo/Red
(A)
Blanco/White
PLMA00169 PLMA00168
1 2
J3
Blanco/White
(E)
(C)
PLMA00043
Rosa/Pink
(K) (B)
1 2 3
J30
Gris/Grey
PELTIER
(A)
Amarillo/Yellow
CELL
MOTOR Z
PLMA00027
Marron/Brown
1 2 3 4
1 2 3 4
Rojo/Red Blanco/White
1 2
J4
J1
Negro/Black Rojo/Red
M
Azul/Blue
PROBE BOARD
CPU BOARD
CIIM00006
CIIM00008
FAN
(E)
Rosa/Pink (C)
(K) (B)
1 2 3
J32
Gris/Grey
(A)
Negro/Black Amarillo/Yellow
1 2
J5
Rojo/Red
MOTOR Y
PLMA00026
PLMA00045
Marron/Brown
1 2 3 4
1 2 3 4
Blanco/White
PROBE
J2
Rojo/Red
M
Azul/Blue
Negro/Black
J6
PLMA00046
(E)
(C)
Marron/Brown Rojo/Red
(K) (B)
1 2 3
3 1 2
1 2 3
J31
J29
J2
(A)
Azul/Blue/Yellow Amarillo/Yellow
PLMA00028 PLMA00038
MOTOR X
Blanco/White
1 2 3 4 5 6
1 2 3 4 5 6
Rosa/Pink Marron/Brown
1 2 3 4
1 2 3 4
J1
Gris/Grey Azul/Blue
J29
J4
Amarillo/Yellow Blanco/White
M
Verde/Green Rojo/Red
Marron/Brown
PLMA00190
PLMA00029
31
Service manual
Esquema de Sistema Fluídico

Fluidic System Scheme
1/2
ELECTROVALVE
2 WAYS
Azul/Blue Negro/Black
1 2
J13
Azul/Blue Rosa/Pink
PLMA00171 PLMA00170
ELECTROVALVE ELECTROVALVE ELECTROVALVE

3MANIFOLD
WAYS
Verde/Green Negro/Black
1 2
J10
Verde/Green Rojo/Red
PLMA00216
PLMA00164
3 WAYS
CPU BOARD
Negro/Black
CIIM00006
Naranja/Orange
1 2
J12
Naranja/Orange Rojo/Red
PLMA00216
PLMA00163
3 WAYS
Amarllo/Yellow Negro/Black
1 2
J9
Amarillo/Yellow Rojo/Red
PLMA00216
PLMA00217
DISPENSING PUMP MOTOR
(E)
(C)
Rojo/Red Rojo/Red
(K) (B)
1 2 3
J33
(A)
Amarillo/Yellow Amarillo/Yellow
PLMA00048 PLMA00004
Negro/Black
1 2 3 4
1 2 3 4
Amarillo/Yellow
M
J5
Blanco/White
Naranja/Orange
PLMA00018
32
Esquema de Sistema Fluídico
Fluidic System Scheme
2/2
SOURCE IN PUMP
Rojo/Red Rojo/Red
1 2
J8
SOURCE OUT PUMP

PLMA00161
Rojo/Red Rojo/Red
J11
1 2
Negro-Amarillo/ Negro/Black
Black-Yellow
PLMA00160
PLMA00162
CPU BOARD
DETECTOR
CIIM00006
SOLUTION
WASHING
INTERIOR
OUTSIDE
A25
A25
Negro/Black Negro/Black Azul/Blue

J44
1 2
Rojo/Rede Rojo/Red
PLMA00167 PLMA00166 Rojo/Red

LEVEL CONTROL GAUGES
Rojo/Red Rojo/Red
3 4
12345678
3 4 1 2
Azul/Blue Azul/Blue
J19
Rojo/Red Rojo/Red
1 2
Azul/Blue Azul/Blue
PLMA00006 PLMA00007
33
Service manual
Esquema de Sistema Rotor

Rotor System Scheme
1/2
SENSOR
ROTOR
Rojo/Red Rojo/Red
COVER
1 2
J24
Marron/Brown Marron/Brown
PLMA00010 PLMA00013
TEMPERATURE
Verde/Green Blanco/White
9 10
SENSOR
J24
ROTOR
Verde/Green Blanco/white
PLMA00010 PLMA00011
FAN
Negro/Black
11 1 2
J24
Rojo/Red
PLMA00010
CPU BOARD
CIIM00006
(E)
(C)
REACTIONS ROTOR MOTOR
Rojo/Red
(K) (B)
6 7 8
Negro/Black
J24
(A)
Amarillo/Yellow
PLMA00010
Negro/Black
1 2 3 4
1 2 3 4
Amarillo/Yellow
J7
Blanco/White
M
Naranja/Orange
PLMA00018
(E)
(C)
Rojo/Red
(K) (B)
3 4 5
J24
Negro/Black
FILTERS WHEEL MOTOR
(A)
Amarillo/Yellow
PLMA00010
Negro/Black
1 2 3 4
1 2 3 4
Amarillo/Yellow
J6
Blanco/White
M
Naranja/Orange
PLMA00020
34
2/2
CPU BOARD DC/DC BOARD
CIIM00006 CIIM00007
J28 J35
J2
1 2 16 pins 1 2 3
Rotor System Scheme
Rojo/Red
PLMA00023
Negro/Black
Gris/Grey
Gris/Grey
PLMA00008
35
PLMA00015
Esquema de Sistema Rotor
16 pins
J3
PHOTODIODE BOARD
CIIM00009
LAMP
(T) (T)
PLMA00009
ROTOR CHASIS A25 CHASIS

ROTOR PELTIER CELL
Service manual
Esquema de Sistema Nevera

Refrigerator System Scheme
J15
TRAY PLATE
Azul/Blue
1
4 3 2 1
PLMA00001
CPU BOARD
CIIM00006
J39
PLMA00192
Blanco/White
J34
16 pins
REFRIG. PLATE
PLMA00023
PLMA00202
Blanco/White
Negro/Black
PLMA00193
Azul/Blue
Rojo/Red
Rojo/Red
J5
1
J7
16 pins
J1
REFRIG.
STATUS
1 2
J2
LED
Rojo/red Rojo/Red
PLMA00196
1 2 3 4
PLMA00191
J4
SENSOR
REFRIG.
COVER
1 2
J8
Rojo/Red Rojo/Red
PLMA00205 PLMA00197
SENSOR
REFRIG.
Verde/Green
RACKS BOARD
1 2
TEMP.
J3
Verde/Green
CIIM00010
PLMA00194
Negro/Black
1 2 3 4 5 6
Rojo/Red
FAN x3
Negro/Black
J12
Rojo/Red
PLMA00199
PLMA00200
PLMA00201
Negro/Black
Rojo/Red
REFRIGERATOR PELTIER CELL x3
Negro/Black
1 2 3 4
J11
Rojo/Red
Negro/Black
Rojo/Red
Negro/Black
1 2 3 4
J10
Rojo/Red
Negro/Black
Rojo/Red
Negro/Black
1 2 3 4
J9
Rojo/Red
Negro/Black
Rojo/Red
36
CPU BOARD
J25 CIIM00006
PLMA00035
USB
J1
CN1
37
Communications Scheme
SERIAL COM1
COMMUNICATION BOARD
Esquema de Comunicaciones
CIIM00019 P1
SERIAL COM2
P2
Service manual
2.11 Schematic liquid circuit
38
3. SERVICE PROGRAM
The service program is used for the adjustment, checking and maintenance of the different components of the
analyzer. It is not supplied with the instrument, it is supplied to authorised technical services only. The personal
of the Technical Service must install it on the user’s computer in order to carry out the service requirements.
Once the tasks have finalised, the program must be uninstalled. To install the program, follow the instructions
on the installation CD ROM called Service. The original password for using this program is A25. The password
can be changed from the service program itself. If the service personnel forget the password, the original pas-
sword can be reinstalled by deleting the hidden file code.a25 from the application directory and relaunching
the program. Once the password has been introduced, the analyzer serial number is given and the name of
the operator is requested (by default Operator1). Press the Accept button and the main program appears. The
different functions of the service program are classified in the following categories:
• Adjustments: These make it possible to make different parameter adjustments required for the correct
functioning of the analyzer.
• Tests: Tests for checking the functionality of the analyzer.
• Utilities: Different technical utilities, such as, for example, washing or priming the dispensing system or
changing an optical filter.
• Registers:This enables the management of past adjustments, tests, incidences, repairs and maintenance
of the instrument.
• Monitor: These enable the low level communication with the analyzer to load new versions of the program
in the flash memory of the analyzer (firmware) or to consult the internal parameters of the instrument.
An emergency stop button (STOP on a red background) will be accessible at all times, and when pressed, it
switches off the analyzer and closes the application quickly.
3.1 Initialising the analyser

To initialise the analyser in service mode, first launch the A25 Service application. The program first of all re-
quests a user or technician ID to be used in the program. Depending on the type of user identified, access to
the different parts of the program will be allowed or denied. The following screen appears:
For full access, enter the following codes:

Name (login): SAT
Password: A25
Once the user has been identified correctly, the service program starts to initialise the analyser.
This screen appears when the analyser has finished the previous operations done to enter the SERVICE
mode. If the complete hardware of the analyzer is in correct conditions, the result “Hardware initiated correctly»
displays.
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Service manual
If any hardware element presents an operational problem, it will appear “Hardware not initiated completely” in
the screen, and the element that is not working correctly will be shown.
In order to close the screen and continue working, you should press the Accept button.
In order to get a printed copy of this initialization report, you should press the Print button.
NOTE: If an error has been reported and the technician continues working with the service program, he must
consider that there is a hardware element that is not working properly.
3.2. ADJUSTMENTS
These make it possible to make different parameter adjustments required for the correct functioning of the
analyzer. All the values to be adjusted have certain limited ranges, indicated by the service program. These
values are also given in an appendix at the back of this manual. If, after varying any of the parameters within
its permitted range, the analyzer is not tuned up, it indicates that the corresponding system is broken and in
need of repair.
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3.2.1. Adjustment of the needle thermostatation system
This screen adjusts the needle thermostatation in such a way that the dispensing temperature of the reactions
is as close as possible to 37ºC. To make this adjustment, the analyzer must be initialised. The liquid to be dis-
pensed is taken from the system liquid container or from the bottle of reagent selected by the technician. The
technician must measure the temperature of the dispensed liquid with a thermometer calibrated at 37ºC. The
program shows the control set point temperature, which is the parameter that must be adjusted for the dispen-
sing temperature to be correct. This parameter must be different from 37ºC. When the technician so indicates,
the analyzer dispenses thermostated distilled water on a certain position in the racks tray shown on the screen.
The technician must measure the temperature of the water with the calibrated thermometer and introduce the
temperature on the screen. The analyzer automatically modifies the set point temperature in accord with the
temperature measured with the thermometer for the dispensing temperature to be 37ºC. The technician can
modify this set point temperature proposed by the program. On pressing Adjust, the analyzer thermostates
the needle with the new set point and, when the technician so requests, performs new dispensing operations.
Each time the set point temperature is modified, wait 1 minute before performing new dispensing operations
for the needle temperature to become stabilised. The technician must repeat this process until the dispensing
temperature is as near as possible to 37ºC. Pressing the Store button, the analyzer stores the current value
of the adjusted set point temperature. Pressing the Cancel button keeps the last stored value and the current
value is not stored. Pressing the Restore button restores the initial screen input value.
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Service manual
3.2.2. Adjustment of the rotor thermostation system
This screen makes it possible to adjust the thermostation system of the rotor in such a way that the reactions
temperature is 37ºC. To make this adjustment, place a well rotor in position and ensure that the analyzer has
been initialised. The rotor can be automatically filled with distilled water by pressing the corresponding button.
Once filled, the technician must wait a few minutes for the rotor to be thermostated. The temperature in the
rotor wells must be measured with a temperature calibrated at 37ºC through the dispensing hole of the rotor
cover. A button makes it possible to turn the rotor in increases of 15 wells to change the well on which the
measurement is being taken. The program shows the control set point temperature, which is the parameter that
must be adjusted for the temperature of the rotor to be correct. This parameter must be other than 37ºC. The
technician must measure the temperature of the water with the calibrated thermometer in the wells and enter
the temperature on the screen. The analyzer automatically modifies the set point temperature in accord with
the temperature measured with the thermometer for the rotor reactions temperature to be 37ºC. The technician
can modify this set point temperature proposed by the program. On pressing Adjust, the analyzer thermostates
the rotor with the new set point. Each time the set point temperature is modified, wait 5 minutes before perfor-
ming new dispensing operations for the rotor temperature to become stabilised. The technician must repeat
this process until the rotor temperature is as near as possible to 37ºC. Pressing the Store button, the analyzer
stores the current value of the adjusted set point temperature. Pressing the Cancel button keeps the last stored
value and the current value is not stored. Pressing the Restore button restores the initial screen input value.
3.2.3. Adjustment of the positioning of the operating arm
This screen makes it possible to adjust the horizontal positioning (X, Y) of the arm. The arm housing must be
removed to see the position of the needle. Before making the adjustments, visually check the verticality of the
needle. If necessary, carefully straighten it up ensuring you do not damage it. On the screen, select the point
at which you wish to adjust the horizontal positioning. On pressing the Adjust button the arm initialises and
positions itself over said point. The technician has buttons to move the arm step by step over the horizontal
plain (X, Y) and vertically (Z). The arm can also be moved introducing a certain number of absolute movement
steps. These absolute movements of the arm must be made with the needle at its highest position so as not to
damage it (coordinate 0). The technician must lower the needle to the adjustment point and adjust its horizontal
position. When the position is satisfactory, save the current coordinates (X, Y) by pressing the Store button.
Pressing the Cancel button keeps the last adjustment values stored. Pressing the Restore button restores the
initial screen input values. At all times, the screen shows the current coordinates of the arm for the selected
point, the last coordinates stored and the initial screen input coordinates, as additional information for the te-
chnician. The technician may repeat the procedure to adjust the positioning of the arm at the different possible
adjustment points. These points are as follows:
1. Origin. Vertex of the self-centering plate of the needle.

2. Rack tray Screw that fastens the washing station cover.
3. Washing station. Centre of the stainless steel font of the washing station.
4. Reactions rotor. Dispensing point on the rotor reactions cover.
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5. Fridge. Dispensation point in the holes of the fridge.
6. XYZ tray position.
If you select the point of origin, automatic adjustment is possible in this position by pressing an AutoAdjustment
button (the process can take around 3 minutes).
3.2.3.1 Adjustment of X, Y and Z position for reagent and pediatric racks
Note: the pediatric rack is a 15 diameter rack with pediatric adapters and wells
This process is used to adjust each rack individually in the three coordinates, X, Y and Z as much for pediatric
as for reagent racks. For that, each rack of the tray will be adjusted to positions 1 and 12 for pediatric racks
and 1 and 10 for the reagent ones.
In order to carry out this XYZ rack adjustment, you may use the screen or keyboard buttons. Movements using
keyboard:
• X axis movements: right and left cursor button
• Y axis movements: up and down cursor button
• Z axis movements: page up and page down button
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Service manual
Adjustment process
1. Select the type of adjustment which you wish to carry out: adjustment of the tray (XYZ) of the pediatric
diameter rack or of the reagent rack.
2. Press the button Adjust.
3. Deactivate the option Adjust with the tool
4. Select the number of rack to start the adjustment process. By default, it starts with number 1.
5. Place the rack in the selected position, place the well or bottle in well number 1 of the rack and press Start.
6. Probe will automatically descend to a distance over the well/bottle, in order to avoid colliding with it. Then,
probe has to be put into the well/bottle just a little. In order to do so:
- If probe is not centered in XY and out of the well or bottle opening: move the probe just by XY movements
(screen or keyboard) before carrying out Z movements to get the probe down.
- Then, get the probe slightly down with Z movements (screen or keyboard) just to make the adjustment
of the center easier.
From this position of approach, you may start the adjustment of values:
7. Adjust the well/bottle center: to do so, move the probe by X and Y movements (screen or keyboard)
8. Adjust the bottle or well bottom: to do so, move the probe by Z movements (screen or keyboard) until it
reaches the bottom. To check it: move the bottle/well up and down.
9. To finish the XYZ adjustment, press Accept.
10.The new adjusted XYZ values will appear in the upper table. (Nevertheless, they are not saved in the ins-
trument yet).
11. The arm will automatically move to position 10 or 12, depending on the type of rack selected. Repeat steps
6 to 9.
12.Once the adjustments in position 10 or 12 are carried out, the arm is parked so the rack can be moved.
13.Place the rack in the following position of the tray, press Start. Repeat steps 6 to 9 to carry out the adjustments
in the every position of the rack tray.
14.Once the adjustments are finished, adjustments have to be kept in the instrument, so press Save.
Adjustment of Z-axis of tubes
When the adjustment of the tray of pediatric racks is selected, it appears another adjustment: the Z relation
between pediatric and tube.
In order to carry out this adjustment, follow the following steps:
1. Place a diameter 15 rack in position 2 of the tray, with a tube in rack position 1.
2. Insert a value in the box of Z pediatric-tube relation. This value shows the separation steps between a pe-
diatric well and a primary tube.
3. Press Start.
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4. Check that the probe has not collided to the bottom of the tube.
5. Move the probe by Z movements (screen or keyboard) until it reaches the well bottom.
6. Once the adjustments are finished, adjustments have to be kept in the instrument, so press Save.
3.2.4. Adjustment of the positioning of the rotor
This screen enables the adjustment of the positioning of the rotor with regard to the dispensing point and the
optical system. One or the other is selected by means of two different tabs.
3.2.4.1. Centering of the rotor with regard to the dispensing point
Put the tool AC15920 in the first well of the rotor. Ensure that the component is positioned until the bottom of
the well. The point of dispensation of the rotor is indicated with the hole of the tool.
The analyzer initialises the rotor and positions the first rotor well at the currently programd dispensing position.
The technician has buttons to move the rotor step by step to adjust, if necessary, this position and buttons for
finer adjustment of the X coordinate over the dispensing point. At all times, the screen shows the current dis-
pensing coordinate on the first well and of the X axis position, the last coordinate stored and the initial screen
input coordinate, as additional information for the technician. When this is satisfactory, the current coordinate
of the dispensing point of the first well can be stored by pressing the Store button. Pressing the Cancel button
keeps the last stored value and the current value is not stored. Pressing the Restore button restores the initial
screen input value.
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Service manual
3.2.4.2. Centering of the rotor with regard to the optical system
This adjustment is necessary only if the Rotor Centering Adjustment has been carried out with regard to the
dispensing point (3.1.4.1.). This adjustment must be made with the rotor cover in position. The analyzer initia-
lises the rotor and fills the first 3 wells of the rotor with distilled water. Next, step-by-step optical readings are
made through these wells at the wavelength selected by the technician. Once the readings have ended, the
program shows a graph of the light intensity measured on the rotor steps. On this graph, the program indica-
tes at which points the optical readings are made on each of the 3 wells when the analysis is made, with the
coordinate of the reading point of the first well currently programd in the analyzer. If necessary, the technician
can move the reading points over the graph jointly using two buttons. The optimum reading point is that which
globally maximises the light intensity for the three wells. At all times, the screen shows the current coordinate
of the reading in the first well and the last coordinate stored, as additional information for the technician. When
the position is satisfactory, the current coordinate of the reading point of the first well can be stored by pressing
the Store button. Pressing the Cancel button keeps the last stored value and the current value is not stored.
3.2.5 . Adjustment of the positioning of the filter wheel
This adjustment must be made with the rotor cover in position. The analyzer initialises the rotor and the filter
wheel and fills the first rotor well with distilled water. Next, it takes optical readings through this well, turning the
filter wheel step by step, with a certain integration time as indicated by the technician (the concept of integration
time is explained in the section on photometric adjustments). Once the readings have ended, the program shows
a graph of the light intensity measured on the steps of the filter wheel. On this graph, the program indicates at
which points each of the filters is positioned when optical readings are taken when the analysis is carried out,
with the coordinate of the positioning of the filter 0 currently programd in the analyzer. If necessary, the technician
can move the reading points over the graph jointly using two buttons. The optimum reading point is that which
globally maximises the light intensity for all the filters. At all times, the screen shows the current coordinate
of the filter 0 and the last coordinate stored, as additional information for the technician. When the position is
satisfactory, the current coordinate of the positioning of the filter 0 can be stored by pressing the Store button.
Pressing the Cancel button keeps the last stored value and the current value is not stored.
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3.2.6. Adjustment of the level control scales
This screen makes it possible to set the level control scales with the empty waste and distilled water containers
(0% capacity) and when they are full (100% capacity). The maximum capacity of the containers is approximately
3L. The technician must choose whether he wishes to set the distilled water or waste container scales, with the
corresponding container full or empty. According to the requested adjustment, the corresponding container, full
or empty, must be placed in position and the Adjust button pressed. Based on the settings made, the analyzer
automatically adjusts the scales. On pressing the Store button, the analyzer saves the new values of the adjus-
ted parameters. Pressing the Cancel button keeps the last stored values and the current values are not stored.
3.2.7. Adjustment of the level detection sensitivity
This screen allows fitting the sensitivity of the capacity level detection system of the probe. The pediatric rack
is a rack with 15mm diameter with the adapter tubes and the pediatric wells inserted.
In order to make the adjustment, first of all you have to select a rack and place on it the following configuration
of racks and samples:
• Paediatric Rack: place 4 paediatric glasses in positions 5, 6, 17 and 18 with 400 uL of liquid system.
• Rack of 13/15mm sample: place 4 tubes in positions 5, 6, 17 and 18 with 400 uL of liquid system.
• Rack of reagents: place 2 bottles of 20mL in positions 5 and 6 with 2mL of liquid system.
• Fridge: Place 2 bottles of 20mL in the position 5 and 6 of the center with 2mL of liquid system.
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Service manual
It is possible to see a graphic with the position of the tubes and racks in the screen photo. When pressing the
Adjust button, the arm takes some sensitivity readings automatically until detecting the water in each one of
the tubes. Once this operation is finished, the arm is parked in its original position and it shows the sensitivity
results of each one of the tubes. Once these readings are finished, the program calculates the average of all
sensitivities; the result is the average sensitivity. The technician should notice that the sensitivity values of each
tube have to be similar; otherwise, he should repeat the whole measurement.
Repeat this adjustment per each rack type.
The sensitivity value can also be introduced manually for each rack in the corresponding box.
Pressing the button Save, the analyser saves the new adjusted sensitivity value. Pressing the button Close,
the old value stays. Pressing the button Restore, the initial value of entry to the screen is restored. A manual
sensitivity value can be inserted in the corresponding box.
3.3. TESTS
Various tests make it possible to check that the different components of the analyzer function correctly.
3.3.1. Motor tests
Through these tests, the technician can check the correct functioning of all the analyzer motors step by step.
The screen makes it possible to choose the motor to be tested and the test that is to be carried out. The analyzer
uses the following motors step by step:
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• X axis of the operating arm.
• Y axis of the operating arm.
• Z axis of the operating arm.
• Dispensing pump
• Rotor
• Filter wheel
All the motor tests can be performed without the covers and housing of the analyzer. After the verifications,
the operating arm always returns to its resting position. To test the motor of the dispensing pump, the arm is
positioned over the washing station. It is convenient for the dispensing system to be primed so that the piston
does not function dry. The following is a description of the different tests that can be performed.
3.3.1.1. Initialization test
This test verifies the start detector of each of the motors.
3.3.1.2. Movement test
This test displaces any of the mobile components to the desired point along its range of functioning, introducing
the corresponding absolute coordinate or moving it step by step. The speed and acceleration of the movement
are those used in the normal functioning of the analyzer.
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Service manual
3.3.1.3. Loss step test
This test makes it possible to check if a motor misses steps when performing a certain sequence of movements.
The test can be carried out with the speed and acceleration used in the normal functioning of the analyzer or
with these magnitudes increased by 10% to check the functioning safety margin.
3.3.1.4. Stress mode test
This test makes it possible for a certain sequence of movements to be performed continually. The technician
can program the duration of the test, which can be cancelled at any moment. Depending on the motor selected,
there is a minimum stress mode time (but in no case is it higher than 50 seconds).
3.3.1.5. Z axis security systems test
The Z axis of the operating arm has an encoder to detect if there have been missed steps as a result of a co-
llision with the needle. In the case of a power failure, a mechanical system automatically raises the needle. On
selecting the corresponding options, the analyzer checks the functioning of each of these devices.
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3.3.1.6 Maximum Z verification test
This test checks that the needle does not collide with the bottles on the rack tray. Select the rack type (reagent,
paediatric, 30 mm or 15 mm), the position of the rack on the tray and the position of the bottle or well on the rack.
Press the Start button to move to the selected position and check if the needle collides with the bottle or well
or if there is space between the needle and the bottle.
Repeat the process in the positions required by the user.
3.3.2. Diaphragm pumps and electrovalves test
The analyzer uses three 3-way electrovalve to manage the dispensing operations. The washing system of
the needle uses a 2-way electrovalve and two diaphragm pumps. The screen makes it possible to choose the
device to be tested and the test that is to be carried out. The devices that can be tested independently are:
• Each of electrovalve of 3 ways of the dosification pump independently

• Electrovalve of 2 way of the washing system
• Each of membrane pump of the washing system independently
To carry out these tests, the dispensing system should be primed. The following is a description of the different
tests that can be performed.
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Service manual
3.3.2.1. Functioning test
This test makes it possible to manually switch the selected device.
3.3.2.2. Stress mode test
This test makes it possible for a certain sequence of device switching to be performed continually. The technician
can program the duration of the test, which can be cancelled at any moment.
3.3.3. Needle self-centering system test
This test makes it possible to check the functioning of the needle self-centering system. During its initialisation,
the analyzer uses this system to check the presence of the needle and its verticality and automatically correct
small deviations. The test consists of simply running this process. The technician can remove the housing of
the arm to observe the test. On the finalisation of the test, the program shows the deviation (x, y) found in the
motor steps.
3.3.4. Needle level detection system test
This test checks the functioning of the system for detecting the capacity of the needle in bottles of reagent and
sample tubes.
This test checks the functioning of the system for detecting the capacity of the needle in reagent bottles and
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sample wells. The test can be performed in any position on the tray.
First select the rack type, then the position of the rack on the tray and, finally, the position of the bottle/well on
the rack. Press the Test button and the program will move the arm to the indicated position and check whether
or not liquid is detected, depending on whether the bottle is full or empty.
Repeat the test as many times as the user considers necessary.
3.3.5. Needle thermostatation system test
This screen makes it possible to check that the dispensing temperature of the reactions is around 37ºC. To make
this adjustment, the analyzer must be initialised. The technician must measure the temperature of the dispen-
sed liquid with a thermometer calibrated at 37ºC. The program shows the set point temperature of the current
control. This parameter must be different from 37ºC. When the technician so indicates, the analyzer dispenses
thermostated distilled water on a certain position in the racks tray shown on the screen. The technician must
measure the temperature of the water with the calibrated thermometer and introduce the temperature on the
screen. The program indicates if the temperature measured is within the tolerated error margins and stores this
value for the test result reports. The liquid to be dispensed is taken from the system liquid container or from the
bottle of reagent selected by the technician.
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Service manual
3.3.6. Rotor thermostatation system test
This screen makes it possible to check that the temperature of the rotor reactions is 37ºC. To make this test, the
analyzer must be initialised. The methacrylate rotor can be automatically filled with distilled water by pressing
the corresponding button. Once filled, the technician must wait a few minutes for the rotor to be thermostated.
The temperature in the rotor wells must be measured with a temperature calibrated at 37ºC through the dis-
pensing hole of the rotor cover. A button makes it possible to turn the rotor in increases of 15 wells to change
the well on which the measurement is being taken. The program shows the set point temperature of the current
control. This parameter must be other than 37ºC. The technician must measure the temperature of the water
with the calibrated thermometer in the wells and enter the temperature on the screen. The program indicates if
the temperature measured is within the tolerated error margins and stores this value for the test result reports.
3.3.7. Photometry tests
This screen contains a set of tests to check the functioning of the optical system. The tests are classified under
different tabs. First of all, the base line and darkness count tests must be made in order to be able to carry out
the remaining tests. To perform these tests, the analyzer must be initialised.
The optical system has a photodiode that generates an electrical current proportionate to the light intensity on
it. time. An AD converter converts the accumulated load into a digital value called count number, between 0
and 1048576. During normal functioning, the analyzer automatically adjusts the integration time for each filter
54
when the analysis begins and after initialisation. When the first photometry test is performed, the integration
times are also automatically adjusted. These times are adjusted in such a way that the count number of the
base line for each wavelength is as near as possible to 950000. In this way, the dynamic range of the detection
system is adapted to the light intensity present at each wavelength. The filter wheel has 10 positions. Position
0 must always contain a covered filter so that the analyzer can perform the darkness adjustment. Positions 1
to 9 can be used for optical filters.
3.3.7.1. Base line and integration times
When this test is run for the first time, the analyzer fills the first 3 rotor wells with distilled water. The analyzer
automatically adjusts the integration times and makes a base line with each of the available filters in each of
the 3 wells. The program shows the current integration times for each of the filters and the average for the 3
wells of the count numbers obtained with each filter. The screen shows the corresponding alarms in the case
of anomaly. It is also possible to access a screen where it is possible to manually vary the integration times
to check their effect on the count numbers. And another screen where it is possible to assign calculated in-
tegration times as reference integration times for each filter. This screen is recommended when a filter or the
lamp is physically changed. After performing the test, the analyzer continues to take optical readings using the
automatically adjusted integration times.
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Service manual
3.3.7.2. Darkness counts
The program shows the current integration times for each filter. On running the test, the analyzer positions the
covered filter and measures the darkness counts with each of the integration times. Each time an optical reading
is taken, the analyzer subtracts these darkness counts from the count numbers measured to obtain the light
intensity. The program shows the values obtained and issues the corresponding alarms in case of anomaly.
The values should be around 4100 - 4300. All the count numbers shown by the tests given as follows have the
darkness counts subtracted.
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3.3.7.3. Repeatability without moving the filter wheel
To perform this and the following tests, the base line and darkness count test must have first of all been perfor-
med. This test takes absorbance readings during 1 minute with the filter wheel in fixed position. The technician
can choose the rotor well on which he wishes to take the readings and fill it with the liquid he desires. He can
choose which wavelength he wishes to use. The test can also be performed with the filter covered. When the
readings end, the screen graphically displays the count numbers obtained and the absorbances with regard to
the corresponding base lines. The program also shows the averages and/or standard deviations of the count
numbers and the absorbances.
3.3.7.4. Stability
This test takes absorbance readings during 30 minute with the filter wheel in fixed position. The technician
can choose the rotor well on which he wishes to take the readings and fill it with the liquid he desires. He can
choose which wavelength he wishes to use. The test can also be performed with the filter covered. The test can
be cancelled at any time. When the readings end, the screen graphically displays the count numbers obtained
and the absorbances with regard to the corresponding base lines. The program also shows the averages and/
or standard deviations of the count numbers and the absorbances.
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Service manual
3.3.7.5. Repeatability moving filter wheel
This test takes absorbance readings during 10 minute moving the filter wheel randomly. The technician can
choose the rotor well on which he wishes to take the readings and fill it with the liquid he desires. The test can
be cancelled at any time. When the readings end, the screen graphically displays the count numbers obtained
and the absorbances for each filter with regard to the corresponding base lines. The program also shows the
averages and/or standard deviations of the count numbers and the absorbances for each filter.
3.3.7.6. Absorbance measurement
This test enables individual absorbance readings. The technician can choose the rotor well on which he wishes
to take the readings and fill it with the liquid he desires. He can choose which wavelength he wishes to use.
The screen shows the count number obtained, the absorbance with regard to the corresponding base line, the
value of the base line.
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3.3.7.7. Reactions rotor check
The user can use this test to check the optical status of a reactions rotor. He or she can choose the optical filter
with which the test is to be performed. The technician must place the rotor in the analyzer and press the Test
button. If the Automatic Fill option has been chosen, the analyzer fills the 120 rotor wells with distilled water
and then makes a base line on each well with the chosen filter. The analyzer graphically displays the absorban-
ces related to the average of all the wells and tells the technician the state of the rotor (optimal, adequate or
unusable). After the test, the user must remove the rotor of the analyzer, empty it and dry it completely before
using it for analyses.
3.3.8. Level control scales test
This screen makes it possible to check the functioning of the level control scales of the waste, distilled water
and the washing solution extern bottle containers. On pressing the Test button, the screen shows the level of
liquid measured by the analyzer. The external bottle container only indicates full (100%) or empty (0%).
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Service manual
3.3.9. Racks and covers detection test

This test makes it possible to check the functioning of the different detectors incorporated in the analyzer.
• Open detector of the general cover of the analyzer.
• Rotor cover presence detector.
• Racks identification detectors
• Fridge detectors. Fridge condition (switch on, switch off), fridge sensor temperature and condition of the
fridge cover.
The technician can manipulate the corresponding components, for example, open and close the cover of the
analyzer and the screen shows the state of the detectors in each case.
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3.3.10. PC-Analyzer communications channel test
On pressing the Test button, the computer attempts to establish communication with the analyzer. The program
tells the technician if it has been possible or not.
The technician can select Automatic Configuration or Manual Configuration. In the case of the latter, he can
define the Port and the Speed.
3.3.11. Global stress mode of the analyzer
This test makes it possible to continually reproduce work cycles of the analyzer similar to those made during
the preparation and reading of reactions in a normal working routine, but dispensing at the washing station
instead of the rotor. It is necessary for the dispensing system to be primed so that the piston does not function
dry. All the racks must be removed from the racks tray. This test can be made without the covers and housing
of the analyzer. The technician can program the number of cycles he wishes (1 cycle = 15 seconds). The test
can be cancelled at any time.
Once the test has been launched, the screen provides regular information about the current status of the pro-
cess. If an error occurs during the process, the test ends and the screen displays a message indicating the
element causing the error.
Partial stressing of the elements of the analyser is possible. The following elements can be stressed partially:
• X axis
• Y axis
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Service manual
• Z axis
• Reactions rotor
• Filter wheel
• Dispensation pump
• Membrane pumps
• 2-way electrvalve
• 3-way electrovalve
3.3.12. Photometry tool
This option is used with the Photometry tool (AC15222). It is used for automate the reading process of the tool.
To use the photometry tool folow the next stetp:
1. Switch on the analyzer.
2. Run the service programme. (The older version to use the tool is the 4.1)
3. Left the analyzer switch on initialized with the service programme for 20 minutes, to preheat the lamp.
4. Select the test menu and Photometry tool option.
5. Press the Load Parameters button.
6. Insert the CD-ROM and select the file ReferenciaUtilFotometria.bin. Push Accept button.
7. Insert the tool (1) in the place of the rotor.
8. Press the Read ABS button.
9. Press the Report button to print the results report.
3.4. UTILITIES
The program contains various technical utilities. These utilities are also accessible from the user program.
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3.4.1. Disassembly of the dispensing needle
On clicking on the Disassemble Needle button, the operating arm positions itself over the rack tray. The program
alerts the technician to remove any object positioned under the arm. On clicking OK, the needle descends
and the technician can remove it to work with it or change it. To remove the needle, unscrew it by holding the
top fitting. If, while handling the needle, the carriage rises due to the pressure made by the technician, press
the Lower Needle button for the needle to descend once again. Once the needle has been reassembled on
the analyzer, press the Park button for the needle to rise. It performs the self-centering test and the arm finally
returns to its parked position. These operations must be done with utmost care since they are carried out with
the analyzer cover open and the needle may be contaminated. Laboratory gloves must always be used.
3.4.2. Prime the fluid system
On pressing the Test button, the analyzer fills the conduits of the dispensing system and the washing station
with distilled water. To perform this operation, the operating arm is moved to the washing station. The technician
can choose whether he wishes to prime the dispensing system, the washing system or both. The technician can
choose which liquid want to prime the dosification system, with air, with washing solution or with liquid system.
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3.4.3. Cleaning of the dispensing system
On pressing the Wash button, the analyzer washes the dispensing system internally and externally. To perform
this operation, the operating arm is moved to the washing station. The technician can choose between perfor-
ming the wash with distilled water or wash solution. In the case of the latter, the analyzer asks the technician
to place a bottle of wash solution in stead of the distilled water container or to fill the latter with wash solution.
Once the wash has been performed, the analyzer asks for the distilled water container to be put back in position.
Finally, the analyzer primes the system with distilled water.
3.4.4. Changing the lamp
When entering the screen, it is possible to choose between: Changing or checking the lamp. When a new
lamp is installed, this utility must be used to notify the analyzer that the lamp has been changed and optimize
the luminosity of the photometric system. The lamp must be changed with the analyzer in sleeping mode. If
the analyzer is on standby mode, the program shuts it down automatically. The lamp must never be touched
with fingers. Once the new lamp has been installed and the covers of the optic and rotor put back, access the
change lamp utility and press the Test button. The program starts up the analyzer, checks the light intensity of
the optical system, shuts down the analyzer and then requests the technician to remove the lamp holder again
and replace it again turning it 180º on the axis of the lamp. If the temperature of the lamp holder is high, wait until
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it cools down or use pincers to hold it. The program starts up the analyzer again, measures the light intensity
of the optical system again, compares the light intensity in both possible positions and chooses the greatest
luminosity. If it is the current position, it tells the technician that the test is complete. If the best position were
the previous one, the program shuts down the analyzer and asks the technician to remove the lamp holder and
replace it, turning it 180º on the axis of the lamp, returning the lamp to its initial position. If the option selected
at the beginning was to Check the Lamp, the process is the same but without shutting down the analyzer at
the beginning.
3.4.5. Configuration of the filter wheel
This screen enables the modification of the analyzer filter wheel. The wheel has 10 positions. Position 0 must
always contain a covered filter so that the analyzer can perform the darkness adjustment. Positions 1 to 9 can
be used for optical filters. All the positions of the wheel must be occupied for it to work correctly. The positions
that do not contain an optical filter must be occupied by a covered filter. The analyzer includes as standard 8
optical filters in positions 1 to 8 and two covered filters in positions 0 to 9. If one of the filters is to be changed,
select the desired position of the wheel and press the Change Filter button. The analyzer automatically posi-
tions the filter wheel appropriately so that the technician can change the filter through the window of the optical
system. Next, if it is different, introduce the wavelength of the new filter that has been installed. If the filter is
covered, introduce value 0. On closing the screen, the analyzer asks if the filters have actually been physically
changed and a series of warnings are given to the technician telling him he must bear in mind whether or not
he has changed a filter.
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3.4.6. Demonstration mode
On pressing the Start button, the analyzer activates some of its mobile components, imitating functioning during
a work routine. The activated mechanical components are the operating arm, the reactions rotor and the filter
wheel. On pressing the Cancel button, the analyzer finishes the current cycle and returns to its rest position.
3.4.7 Read/load adjustments and cycles
From this screen, it is possible to read the current adjustments that the analyser is using by pressing the button
Read Adjustments.
It is allowed to save these adjustments in a file. The technician selects the name and location of this file.
Also from this same screen and with the button Load Adjustments, the technician is allowed to select an ad-
justment file and to load it in the analyzer. Once the adjustment loading is made, the analyser turns off and
the application is closed. When reinitiating the application, the new loaded adjustments will be already active.
From the firmware version 2.80, the programme counts the number of cycles of each element and the task of
the analyser. From this menu, it is possible to read the cycles completed by the analyser. The screen displays
the said cycles with the corresponding units.
The programme automatically saves a copy of the adjustments and cycles read in a file. This file is located in
the following folder:
c:\Program files\A25 Service\Adjustments\
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When a physical element of the analyser has to be changed, e.g. the Z axis belt, the counter must be reset
to zero for it to correspond to the number of cycles actually stored in the analyser. To perform this operation,
select the box of the element that is to be initialised and enter the number of cycles in the enabled box. Then
press the Load cycles button.
Using the Load adjustments button, this screen also enables the technician to select an adjustments file and load
it in the analyser. When the adjustments are loaded, the cycles are also loaded. Perform this operation when
a CPU board has to be changed. This avoids having to completely readjust the analyser; only the following
sections will have to be readjusted:
• Scales
• Level detection sensitivity
• Needle thermostatation
• Rotor thermostatation
3.4.8 Change the rotor type
In this screen the type of rotor is introduced. Each rotor comes labelled with a letter in its top part. Select in
this screen the type of rotor to use. For rotors marked with A letter, only select the letter. For the rotors marked
with other letters, select OTHERS and then introduce the light path that will come it within the box of rotors or
of the distributor.
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3.5. REGISTER
This enables the management of past adjustments, tests, incidences, repairs and maintenance of the instrument.
3.5.1. Introducing the analyzer serial number
The technician can enter the analyzer serial number so that it appears on printed service reports. If an ente-
red serial number is changed, the service records are reinitiated. In this case, the technician can store all the
previous data in a file.
The technician can enter his name so that it appears on the printed service reports.
3.5.2. Service Reports
The program can display and print various service reports. The printed reports contain the analyzer serial
number and the name of the current technician.
Reports are stored organised by: Adjustments, Tests, Utilities, Monitor and Summary of actions and tasks
carried out.
In all cases, it is possible to select the actions carried out within a range of dates chosen by the technician.
The technician can enter short descriptions of the incidences that may happen in the analyzer and the repairs
and maintenance operations that may be performed to the instrument in the Observations box.
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3.5.3. Language change
This makes it possible to choose the language used in the service program.
3.5.4. Users
Two types of user can be created with different access levels:
· SAT. This user has full access to the programme. This user has permission to create and/or delete
other users.
· User. This user has restricted access to the programme. This user can only perform the tests and run
the utilities. He/she can not make any adjustments or load any previously saved adjustments files or change
the firmware version of the analyser.
From the Users menu, it is possible to create, delete and change users. The Change password option is for
each user to change his own password.
3.6. MONITOR
These enable the low level communication with the analyzer to load new versions of the program in the flash
memory of the analyzer (firmware) or to restore the default adjustment parameters.
The firmware of the analyzer resides in a permanent flash memory. The change of this program can be made
through the computer without the need for changing the memory chip.
To load the new version, press the Start button, previously indicating where the program is located using the
Open button.
From version 3.0.2, before loading a new firmware, the program checks that the file is correct. If is so, the
program sends it directly to the analyzer. If there is an error message, please contact the Technical Service to
replace/recover the corrupt file
First of all, the current content of the flash memory is deleted and then the new program is loaded. This ope-
ration may take several minutes.
Once the program has changed, the analyzer is restarted with the new version of the program. While the copy
process is being performed, the screen indicates the percentage completed.
There is also the option to Restore Default Adjustments, selecting the option and pressing Start.
If the technician wants the analyzer to enter monitor mode (e.g. because the analyzer does not respond be-
cause the firmware was incorrectly loaded, he may do so by shutting down the analyzer, pressing the Force
Monitor button and then rebooting.
Once the new programme has been loaded or the default adjustments have been restored, exit the monitor by
pressing the Close button.
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3.7 User’s program

In this section, the service options in the user program will be described. These options are intended to confi-
gure the user’s access level.
Each section explains how to manage and create different levels of access to the user program of the analyser.
When the program is installed for the first time, there is not a created user and access to the program is complete.
3.7.1 Configuration of the level of access to the analyser
To activate the option of level of access to the analyser, the first time you should enter as administrator, whose
values are:
Name of user: admin

access key: a25
with this screen, the application with the operation by passwords is configured.
The first time that the program is activated, it forces the user to change the initial password.
It is possible to create three types of user with different access levels:
• Operator, is the user with a lower level of access to the application. He can only do working sessions, reports
of current and historical results, and validate quality control results. In the screens of programming of techniques
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and contaminations, he can look up programming values, but he can not modify any parameter. He can not
delete results or alarms. This user has total access to the rack and profile programming and to the analyser’s
configuration (except for changes of filters). He can change his own password.
• Supervisor, is the user with a medium access level. This user has got the same privileges as the operator
user’s and, in addition, he has got permissions to modify the programming of techniques in the calibration
parameters and the control values. He can create a restricted number of new techniques, that is defined at
the moment of creating such user and that it is a default setting of 5. He can also modify the programming of
contaminations and change the analyser’s filters. He can change his own password.
• Administrator, is the user with total access to the analyser’s functions. He can create new users -as much at
supervisor as at operator level-, eliminate or modify users. When creating supervisor users, he has to indicate
the maximum number of new techniques that can create. He can activate or deactivate Work Without Passwords
(option within the Configuration menu). He can also activate/deactivate the working without cover detection
(this option is useful for the technical service to make verifications without needing to let the cover down). This
option activates solely when the passwords are active.
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When users are created, the access is limited to different parts of the program. When starting the program, an
identification of the user is requested, by the user name and a password, and then the program will automatically
restrict the different parts of the program depending on the access level permitted.
Whenever you want, you can change the user by means of the option Change of user from the User menu.
It is also allowed to eliminate users already created. Each user is capable of changing his password. All these
options can be reached from the user menu.
3.7.2 Reagent Consumption
In order to access the consumption of reagents, it is first necessary to configure the program with the option
of working with passwords. The administrator user is the only one that can access this menu -this option is
deactivated for any other user.
In order to generate a list of the consumption of reagents, the administrator has to introduce the dates between
which he wants to know the consumption. For this, it appears a screen like this:
Such option creates two files of results, one in text format .txt and the another one in excel format .xls. These
files will be located at directory within the application directory, it will usually be:
c:\Archivos de programa\A25\Reagents
and the contents of the file shows similar this:
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REAGENT CONTROL CONSUME REPORT
Initial Date: 02/11/2004 Final Date: 02/12/2004

Test Blank Prep. Calibrator Pre Control Prep. Patient Prep. Total Prep. Vol. R1 (uL) Vol. R2
(uL)
glucose 1 0 0 5 6 1332 0
alt 1 0 0 3 4 888 0
bilirrubin 9 0 0 23 32 7104 3552
Reagents
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Service manual
4. RECOMMENDATION
4.1. RECOMMENDED PREVENTIVE MAINTENANCE

It is advisable to carry out at least the following actions for weekly maintenance:
Clean the outside of the end with 70º alcohol, use the utility of end change to access it.
It is recommended that the following maintenance actions are performed annually or every 1,000 working hours.
Operating arm
1. Check the state and stress of the belts.
2. Replace the components in an unsatisfactory state.
3. Make the adjustments and tests related to the service program.
Dispensing system.
1. Exhaustively wash all the dispensing circuit with washing solution and rinse it with distilled water.
2. Remove and clean the needle. Check its state.
3. Check the state of all the dispensing circuit tubes. Check the absence of obstructions or diameter changes.
4. Check the leakproof quality of the fittings and the 3-channel electrovalve body set.
5. Check the leakproof quality of the dispensing pump fitting and seal. Check there is no loss and no bubbles.
6. Clean the distilled water container filters.
7. Clean the washing station.
8. Check the state of the washing system tubes.
9. Check the leakproof quality of the fittings and the 2-way electrovalve body.
Reactions rotor
1. Check the state of the heating channel.
2. Check the belt stress.
Optical system
1. Check the state of the lamp.
2. Clean the filters.
3. Clean the lenses.
4. Clean the photodiode.
4.2. CARE AND CLEANING

4.2.1. General care of the analyzer
a) Never use detergents or abrasive products for cleaning the surface of the analyzer. Use only a damp cloth with
water and pH-neutral soap.
b) If a reagent or corrosive product spills or splashes onto the apparatus, clean it with a damp cloth and soap imme-
diately. If necessary, protect your hands with appropriate laboratory gloves.
c) All the components of the analyzer have drainage conduits leading to the exterior to enable the elimination of any
liquid spilled and to prevent the apparatus from flooding. If the spillage is significant, the liquid spilled onto the table
through the drainage conduits and the analyzer must be adequately cleaned.
d) When not in use, close the main cover of the analyzer to protect it from dust.
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4.2.2. Cleaning the optical system
The components of the optical system must be cleaned periodically in order to keep them free from dust and dirt.
These components are the lamp, the lenses, the filters and the photodiode. The recommended necessary material
is as follows:
- Special paper for cleaning optical components (non-abrasive paper which does not leave solid residue).
- Ether and alcohol solution at 50%
- Cotton wool buds.
- Small bellows.
All the optical components must be handled in an area of maximum cleanliness and with great care given that they are
fragile and delicate. To remove them and refit them, the corresponding instructions given in the Maintenance chapter
must be carefully followed. Avoid touching the useful area of these components with fingers. The lenses, filters and
the photodiode must be held by the sides. Do not touch the lamp bulb. To handle the lamp, use the wrapping, cutting
it at the terminal ends and squeezing them until they come out. To clean the optical components, bear in mind the
following:
a) Remove the dust on the surface of the component with the bellows. This will avoid scratching by particles when
cleaning with paper.
b) Carefully clean the surface of the component with the cleaning paper.
c) If the dirt is persistent or greasy, clean the component with the paper moistened with the alcohol and ether solution.
Then dry with dry paper. To clean the filters or the window of the photodiode, use the cotton wool buds with the
paper in difficult and delicate areas.
d) Finally, it is recommended that you use the bellows once again, thus removing any remains of paper or cotton.
4.2.3. Cleaning the dispensing system
The analyser automatically performs conditioning of the fluid system at warm-up and shut-down. This conditioning
consists of a 3 primings of the dosing system, the first with air, the second with washing solution and the third and
last with system liquid. To perform this conditioning, the bottles of system liquid (marked with a blue dot) and washing
solution (marked with a green dot) must be full, and the waste bottle (marked with a red dot) must be empty. The final
conditioning consists of performing a priming with washing solution.
With initial wash, the system is ready to work in optimum conditions for the whole workday, offering its best performance.
With the final wash, the analyser cleans the tip at the end of the day’s work, thus keeping it in perfect condition for the
next workdays. The user can also perform conditionings of the dosing system when desired by pressing the conditioning
button in the vertical button bar on the monitor screen while the analyser is in standby mode. It is also recommended
to clean and check the status of the filters of the system liquid container at least once every 3 months.
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Service manual
If the needle is obstructed by solid residue, it must be removed and cleaned using the metal cleaning rod supplied
with the analyzer. For this, the Remove the dispensing needle utility should be used. It is also recommendable to
clean the outside surface of the needle with a piece of cotton or a soft cloth dampened with alcohol. The needle must
be replaced if it is noticeably deteriorated.
4.2.4. General cleaning of the interior of the apparatus
It is important for the interior of the instrument to be free from dust at all times in order to preserve the correct functio-
ning of the different components. For this, remove the front housing of the analyzer and carefully clean the dust inside
the instrument.
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A I. TECHNICAL SPECIFICATIONS
PLEASE NOTE
The manufacturer accepts no liability for damage caused by incorrect use of the apparatus.
GENERAL SPECIFICATIONS
Automatic random and continual access analyser aimed at giving results per patient, with direct photometric reading
over a reactions rotor.
Preparation cycle time 15 s (up to 240 prep/h)

Warm-up time 25 min
Reading time for each preparation Every 15 s, up to 15 mins
Size 1080 x 695 x 510 mm (42.5”x27.4”x20.1”)
(length x depth x height)
Weight 70 kg (154 lb)
REAGENTS AND SAMPLES TRAY

Positions for unrefrigerated racks 3
Positions for racks 3
Capacity of refrigerated zone 30 reagent bottles (20 or 50mL)
Capacity of the sample racks 24
Maximum number of samples 72
ø13 mm, ø15 mm sample tubes (max. height 100 mm), ø13 mm paediatric well
Capacity of the reagent racks 10
Maximum number of reagents 50 (plus 3 auxiliary fixed positions)
20 ml and 50 ml reagent bottles
Possible configurations
Sample racks Reagent racks Number of samples Number of reagents

1 2 24 50 (30 refrigerated)
2 1 48 40 (30 refrigerated)
3 0 72 30 refrigerated
REAGENT REFRIGERATION
Refrigeration capacity 15°C below room temperature when this is 25°C
Electrical power of refrigeration 75 W
Power supply separate from analyser. Dedicated power supply and switch.
Open lid sensor and LED power indicator of refrigerated zone.
DISPENSING SYSTEM
NEEDLE
Detachable tip
Vertical length 110 mm
Capacity level detection
Self-adjustment of position
NEEDLE THERMOSTATATION SYSTEM

Actuator 1 Peltier cell
Control Fuzzy Logic
Dispensation temperature 37°C
Repeatability ± 0.5°C
DISPENSING PUMP
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Service manual
Ceramic piston with PTFE-graphite seal

Piston diameter 8 mm
Dispensing volume 3 µL - 1250 µL
Programmable reagent volume 10 µL - 440 µL
Programmable sample volume 3 µL - 40 µL
NEEDLE WASHING SYSTEM

System liquid consumption approx. 5 ml per preparation
System liquid container volume 2700 mL
Waste container volume 2700 mL
Washing solution container volume 2700 mL
Waste and water level control per weighing (load cells)
AUTOMATIC CONDITIONING SYSTEM OF SYSTEM FLUID

A system of valves that enables the fluid dispensing system to be filled with system liquid, air or washing solution.
3L bottle of washing solution external to instrument equipped with minimum level detector.
Connections of bottle of washing solution with quick antidrip plug for liquid and jack connector for signal level. Both
on the back panel of the instrument.
REACTIONS ROTOR AND READING
WELL ROTOR
Semi-disposable extractible methacrylate rotor
Number of wells 120
Accepted reaction volumes 200 µL - 800 µL
Light path length 6 mm
ROTOR THERMOSTATATION SYSTEM

Actuators 4 Peltier cells
Control PID
Working temperature 37°C
Trueness ± 0.2°C
Stability ± 0.1°C
OPTICAL SYSTEM
Halogen lamp 12 V, 20 W
Wavelength selection with compensated interferential filters
Detection system with silicon photodiode and 20-bit AD integrator-converter
Measurement range from -0.05 A to 3 A
Reading speed 5 readings/s
Maximum number of filters 9
Base configuration of the filter wheel 340, 405, 505, 535, 560, 600, 635, 670 nm
Wavelength precision ± 2 nm
Bandwidth 10 ± 2 nm
Digital resolution < 0.0001 A
Base line stability max. 0.004 A in 30 mins, at 505 nm
MINIMUM COMPUTER REQUIREMENTS

Pentium IV processor or higher
Windows XP o Vista
512 Mbytes RAM
20 Gbytes free hard disk space
CD-ROM
VGA monitor, minimum screen resolution 800x600
mouse
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RS-232 serial channel connector or USB
The insulation level of the A25 analyser communications channel is reinforced (the insulation of the communications
channel of the computer has also been reinforced)(1)
Reinforced insulation is that which ensures protection that is equal to or higher than double that provided by the
(1)
main insulation. The main insulation is that whose failure could lead to the risk of electric shock (UNE EN 61010-1).
CHECKED REQUIREMENTS OF THE CODE BAR READER

Reading Speed 200 readings / second
Reading Width 80 mm
Resolution 0.1 mm
Light Source Visible red LED of 660 nm
Sensor Linear CCD of 2160 elements
Input Voltage 5VDC
Interface Keyboard (PS/2 and AT), USB, RS232C
POWER REQUIREMENTS
Input voltage 115-230 Vac, 50/60 Hz
Power 300 VA
Electrical installation category (overvoltage category) II
The mains power point must be approved and have an earth connection and cable with a minimum section of 1.5
mm2.
ENVIRONMENT CONDITIONS
Interior use
Altitude < 2500 m
Temperature 10°C - 35°C
Relative humidity <75%
Contamination level 2
COMPLIANCE WITH DIRECTIVES AND APPLIED STANDARDS

Directive 98/79/EC related with In Vitro Diagnostic products
• UNE-EN 61010-1:2002+CORR:2003 “Safety requirements for electrical equipment for measurement, control and
laboratory use. Part 1 - General requirements”
• UNE-EN 61010-2-101:2004 “Particular requirements for In Vitro Diagnostic (IVD) medical equipment”
• UNE-EN 61326-2-6:2006 “Electromagnetic equipment for measurement, control and laboratory use –ECM requi-
rements. Part 2: Particular requirements. In vitro diagnostic (IVD) medical equipment”.
• UNE-EN 55011:1999+A1:2000+A2:2003 “Limits and methods of measurement of radio disturbance characteristics
of industrial, scientific and medical (ISM) radio-frequency equipment”. Continuous conducted: Class A Radiated:
Class A
• UNE-EN 55014:2002+A1:2002+A2:2004 “Electromagnetic compatibility. Requirements for household appliances,
electric tools and similar apparatus. Emission”
• UNE-EN 61000-3-2:2006 “Electromagnetic compatibility (EMC) – Part 3: Limits –Section 2: Limits for harmonic
current emissions”.
• UNE-EN 61000-3-3:1997+A1:2002+A2:2006 “Electromagnetic compatibility (EMC) – Part 3: Limits –Section 3:
Limitation of voltage fluctuations and flicker in low-voltage supply systems”.
• UNE-EN 61000-4-2:1997+A1:1999)+A2:2001 “Electromagnetic compatibility (EMC) – Part 4: Testing and measu-
rement techniques – Section 2: Electrostatic discharge immunity test”.
• UNE-EN 61000-4-3:2003+ERR:2003+A1:2004 “Electromagnetic compatibility (EMC) – Part 4: Testing and mea-
surement techniques – Section 3: Radiated, radio-frequency, electromagnetic field immunity test”.
• UNE-EN 61000-4-4:2005 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques –
Section 4: Electrical fast transient/burst immunity test”.
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Service manual
• UNE-EN 61000-4-5:1997+A1:2001 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement

techniques – Section 5: Surge immunity test”.
• UNE-EN 61000-4-6:1998+A1:2001 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement
techniques – Section 6: Immunity to conducted disturbances, induced by radio-frequency fields”.
• UNE-EN 61000-4-11:2005 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques
– Section 11: Voltage dips, short interruptions and voltage variations immunity tests”.
• UNE-EN 61000-4-8:1996+A1:2001 “Electromagnetic compatibility (EMC). -Part 4: Testing and measurement te-
chniques. Section 8: Power frequency magnetic field immunity test.”.
• UNE EN 22233-1992 (ISO 2233-1986). Packaging — Complete, filled transport packages and unit loads — Con-
ditioning for testing
• UNE EN 24180-2-1992 (ISO 4180-1980). Complete, filled transport packages — General rules for the compilation
of performance test schedules.
• UNE EN 22247-1992 (ISO 2247-2000). Packaging — Complete, filled transport packages and unit loads — Vibra-
tion tests at fixed low frequency
• UNE EN 22248-1992 (ISO 2248-1985). Packaging — Complete, filled transport packages — Vertical impact test
by dropping
MAXIMUM SIZE OF ANALYZER
The manufacturer reserves the right to modify any technical specification without prior notice.
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Additional Information about device security
The special “waste container crossed out” symbol indicates that the product in question
is subject to the selective waste collection principle as established in European Union
Directive WEEE (Waste Electrical and Electronic Equipment).
Once the instrument’s lifetime is completed it becomes waste and, as established in the
directive, the said waste must be separated from domestic waste for its proper recycling.
To this end, the manufacturer facilitates its elimination.
The analyser complies with the requirements for immunity and emissions specified in the
standard UNE-EN 61326-2-6:2006.
This equipment was designed and tested for Class A of the standard UNE-EN 55011:1999.
It may cause radio interference in a home environment, in which case it may be necessary
to take measures to mitigate the interference.Do not use the analyser in the proximity of
sources of strong electromagnetic radiation (such as centrifugation devices, radios, cell phones), as these may inter-
fere with its proper functioning.
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Service manual
A II. ADJUSTMENT MARGINS TABLES

Main voltage measurement points Positioning of XY fridge adjustment
CIIM00007 Power supply board X fridge [1700, 1730]
TP1- 5V [4.9-5.1] V Y fridge [1285, 1315]
TP2 - 12V [11.7 -11.9] V Positioning of the dispensing point
Dispensing rotor [-10,30]
TP3 - 12V [11.8-12.2] V
Fine rotor X [2860,2897]
TP4 - 12V [11.8-12.2] V
Positioning of rotor in optic
TP5 - 12V [11.8-12.2] V
Positioning [-12,6]
TP36 [32-40] V
Filter wheel positioning
Positioning [-6,0]
CIIM0006 Microprocessor board Needle thermostatation
TP50 - Vref Z motor [3-4] mV Set point temperature [35.5,39.5]
TP51 - Vref Ymotor [3-4] mV
Rotor thermostatation
TP52 - Vref Xmotor [3-4] mV
Set point temperature [36.5,38]
TP53 - Vref pump motor [3-4] mV
Integration times
TP54 - Vref filter wheel motor [3-4] mV
F1 340 nm [30,70]
TP55 - Vref rotormotor [3-4] mV F2 405 nm [6,26]
F3 505 nm [6,26]
Main adjustment values of the analyzer F4 535 nm [6,26]
Tolerances F5 560 nm [6,26]
Operating arm positioning F6 600 nm [6,26]
X axis loss of steps F7 635 nm [6,26]
Loss of steps [-3,3] F8 670 nm [6,26]
Y axis loss of steps Number of counts
Loss of steps [-3,3] F1 340 nm [750000,960000]
Z axis loss of steps F2 405 nm [840000,940000]
Loss of steps [-3,3] F3 505 nm [840000,940000]
Loss of steps rotor motor F8 670 nm [840000,940000]
Loss of steps [-3,3] Darkness counts
Loss of steps filter drum motor F1 340 nm [3700,4300]
Loss of steps [-3,3] F2 405 nm [3700,4300]
Loss of steps pump motor F3 505 nm [3700,4300]
Loss of steps [-4,4] F8 670 nm [3700,4300]
XY position adjustment SMF repeatability (Noise)
X origin [5,30] Dev. Est. NC. F0 Covered ≤ 55
Y origin [0,45] Dev. Est. Abs. F1 340 nm ≤ 0.0004
X washing station [1325,1360] Dev. Est. Abs. F2 405 nm ≤ 0.0006
Y washing station [0,35] Dev. Est. Abs. F3 505 nm ≤ 0.0004
X rotor [2855,2] Dev. Est. Abs. F8 670 nm ≤ 0.0007
Y rotor [660,700] Stability at 505 nm
Self-centering of needle Dev. Est. Abs. F3 505 nm ≤ 0.0008
X Offset [-4,4] Max. NC [840000,940000]
Y Offset [-4,4] Min. NC [840000,940000]
Level control scales Max NC / Min NC ≤ 1.006
Water 0 L (0 D) [-3%,3%] Repeatability MF (Repeatability)
Water 1.35 L (1 D) [35%,65%] Dev. Est. Abs. F1 340 nm ≤ 0.0008
Water 2.7 L (2 D) [95%,105%] Dev. Est. Abs. F2 405 nm ≤ 0.0006
Waste 0 L (0 D) [-3%,3%] Dev. Est. Abs. F3 505 nm ≤ 0.0005
Waste 1.35 L (1 D) [35%,65%] Dev. Est. Abs. F4 535 nm ≤ 0.0005
Waste 2.7 L (2 D) [95%,105%] Dev. Est. Abs. F5 560 nm ≤ 0.0005
Level detection sensitivity Dev. Est. Abs. F6 600 nm ≤ 0.0005
Paediatric rack [25,100] Dev. Est. Abs. F7 635 nm ≤ 0.0005
13/15mm tube sample rack [45,100] Dev. Est. Abs. F8 670 nm ≤ 0.0005
Reagent rack outside fridge [10,60]
Reagent rack inside fridge [10,60]
82
A III. LIST OF CONSUMABLES, ACCESSORIES AND
SPARES
If any of the components of the analyzer deteriorate of if any of the perishable materials are required, always use
original BioSystems material. The following table shows lists of components that may be required. To purchase said
components, please contact your usual distributor and order each component using its corresponding code. This will
simplify work and minimise errors.
List of user spares, perishable materials and accessories.
CODE DESCRIPTION(Sort by code, numerical part) CODE DESCRIPTION (Sort by description)

LA10418 12 V/20 W halogen lamp LA10418 12 V/20 W halogen lamp
CA10455 European mains cable AC14550 15 mm tube sample rack
CA10456 American mains cable AC12223 2 mm Allen key
FI10466 Serial channel cable for connection to PC AC12224 2.5mm Allen Key
AC10770 Samples wells(1000) BO11494 20 ml bottle with top (10 units)
AC11484 User program CD AC14549 20 ml/50 ml reagents rack
AC11485 Reactions rotor (10 units) FI11563 340 nm filter unit
AC11486 Reactions rotor fastening screw FI11564 405 nm filter unit
BO11487 System liquid container with top BO11493 50 ml bottle with top (10 units)
FI11488 System liquid container filters FI11565 505 nm filter unit
BO11489 Waste container with lid and fitting FI11490 535 nm filter unit
FI11490 535 nm filter unit FI11491 560 nm filter unit
FI11491 560 nm filter unit FI11566 600 nm filter unit
FU11492 Set of 4 A (F) fuses FI11567 635 nm filter unit
BO11493 50 ml bottle with top (10 units) FI11568 670 nm filter unit
BO11494 20 ml bottle with top (10 units) CA10456 American network cable
AC11495 Access filter cover BO11524 Bottle of concentrated system liquid (1L)
AC11496 Optical system cover with screws BO13416 Bottle of concentrated washing solution
AC11497 Reactions rotor cover (100 mL)
FI11498 Covered filter unit FI11498 Covered filter unit
ZO11499 Lamp holder fastening system AC11500 Detachable needle
AC11500 Detachable needle CA10455 European network cable
AC11505 Operating arm fastening for transport AC14553 Fridge grid
AC11506 Height-adjustable leg AC11506 Height-adjustable leg
BO11523 Washing solution container with top AC14555 Instalation manual: ES-EN-FR-IT-PT
BO11524 Bottle of concentrated system liquid (1l) AC14556 Instalation manual:EL-RO-RU-PO
FI11563 340 nm filter unit AC11495 Interior optical system cover
FI11564 405 nm filter unit ZO11499 Lamp holder fastening system
FI11565 505 nm filter unit AC12222 Metal rod for cleaning the needle
FI11566 600 nm filter unit AC11505 Operating arm fastening for transport
FI11567 635 nm filter unit AC11496 Outside optical system cover with screws
FI11568 670 nm filter unit AC11485 Reactions rotor (10 units)
AC12222 Metal rod for cleaning the needle AC11497 Reactions rotor cover
AC12223 2 mm Allen key AC11486 Reactions rotor fastening screw
AC12224 2.5mm Allen Key AC10770 Samples wells (1000)
BO13416 Bottle of concentrated washing solution (100 mL) FI10466 Serial channel cable for connection to PC
FI14226 USB cable for connection to PC FU11492 Set of 4 A (F) fuses
AC14549 20 ml/50 ml reagents rack FI11488 System liquid container filters
AC14550 15 mm tube sample rack BO11487 System liquid container with top
AC14553 Fridge grid AC14554 Tube adapter (80 units)
AC14554 Tube adapter (80 units) AC14565 Tubes for bottle of washing solution with float
AC14555 Instalation manual: ES-EN-FR-IT-PT FI14226 USB cable for connection to PC
AC14556 Instalation manual:EL-RO-RU-PO AC11484 User program CD
AC14565 Tubes for bottle of washing solution with BO11523 Washing solution container with top
float BO11489 Waste container with lid and fitting
83
Service manual
LA10418 CA10455 CA10456 FI10466 AC10770 AC11484
535
AC11485 AC11486 BO11487 FI11488 BO11489 FI11490
560
Fi11491 FU11492 BO11493 BO11494 AC11495 AC11496
AC11497 FI11498 ZO11499 AC11500 AC11505 AC11506
340 405 505 600
BO11523 BO11524 FI11563 FI11564 FI11565 FI11566
635 670
FI11567 FI11568 AC12223 AC12224 AC12446 BO13416
FI14226 AC14549 AC14550 AC14553 AC14554 AC14555
AC14556 AC14565
84
List of spares exclusive to the technical support service.
CODE DESCRIPTION(Sort by code-numerical part) AC13304 ROTOR HOLDER
VA10355 MAINS CONNECTOR F370 ZO13309 LAMP HOLDER FOR TURBI.SYSTEMS
ZO10407 FUSE HOLDERS PACK AC13310 ROTOR GEAR
ME10490 FAN´S GRILL ME13359 DISPENSING PUMP GROUP
ME11513 Y CARRIAGE BELT AC13361 DISPENSING PUMP SEALS KIT
ME11514 Z CARRIAGE BELT AC13362 DISPENSING PUMP FLUID. CHAMBER
MO11516 Y MOTOR MO13363 DISPENSING PUMP MOTOR with ceramic
AC11519 ENCODER-SPRING WITH Z MOTOR piston
AC11520 THERMOSTATED NEEDLE SET AC14213 Y AND Z LOOSE PULLEY
AC11521 THERMISTOR PROBE AC14225 PROBE O-RING
MO11522 60mm FAN PROVE AC14307 DISPENSING PUMP TEFLON TUBE
TU11531 OPERATING ARM TEFLON TUBE AC14463 POWER SUPPLY-CPU CABLES
TU11533 CONTAINER TUBING SET AC14619 encoder spring
AC11534 WASHING SYSTEM DIAPHRAGM PUMP MO14620 X MOTOR
AC11536 WASHING SYSTEM FLOW RESTRICTOR ME14628 X CARRIAGE BELT
TU11537 WASHING SYSTEM PVC TUBES ME14670 Manifold 3 way electrovalve
AC11538 WASHING STATION COVER ME14671 2 WAY ELECTROVALVE WITH NUTS
AC11539 WASTE CONTAINER NUT AND CAP AC14706 RACKS SUP.TRAY WITH METAL BASE
CE11540 LOAD CELL AC14707 REACTION ROTOR GROUP
AC11542 ROTOR TEMPERATURE SENSOR AC14708 A25 LENS SET
ME11543 ROTOR BELT PC14709 RACKS DETECTION BOARD
MO11544 ROTOR MOTOR AC14710 X TRACK RAIL WITH 2 SLIDE UNIT
AC11545 ROTOR COVER DETECTOR AC14711 Y TRACK RAIL WITH 1 SLIDE UNIT
AC11546 FILTER WHEEL BODY AC14712 Z TRACK RAIL WITH 1 SLIDE UNIT
MO11547 FILTER WHEEL MOTOR AC14713 EPROM UPDATE 83101
ME11549 HYDRO-PNEUMAT.CYLIND.TOP COVER AC14714 X LOOSE PULLEY
CA11550 ARM HOUSING AC14715 TEST BOARD EPROM A25
PC11551 MICROPROCESOR BOARD PC14716 POWER SUPPLY instrument
PC11552 PHOTOMETRIC BOARD FO14717 photodetector autocenter probe (2)
PC11553 NEEDLE CONDITIONING BOARD AC14718 waste DIAPHRAGM PUMP
PC11555 FRONT INDICATOR BOARD AC14719 fridge O-ring
PC11556 POWER SUPPLY BOARD AC14720 fridge fan
IN11557 MAINS SWITCH AC14721 fridge cover
MO11558 FAN 80 mm AC14722 fridge hinge
AC11559 WINDOW TOP COVER AC14723 fridge led
CE11561 probe peltier cell AC14724 fridge cover detector
FO11562 PHOTODETECTOR SET (5) AC14725 fridge power supply
AC11572 BASE HOUSING AC14726 fridge temperature sensor
AC11573 REAR HOUSING ZO14727 instrument holder
AC11574 SET HINGE (PACK 2) ME14728 electronic cover hinge
AC11580 UPPER HOUSING BO14729 external botlle in-out coupling
AC11860 adjust temperatura tool AC14731 CD SERVICE SOFTWARE 83101 with service
manual
PC11862 COMMUNICATION BOARD
CE14757 rotor peltier cell
AC13079 REFILL CERAMIC PUMP
CE14758 FRIDGE PELTIER CELL
AC13082 CONECTORS &PINS FOR A25
AC14763 TOOL LENS
AC13113 ARM CABLES A25
AC13227 SPIRAL SET
85
Service manual
List of spares exclusive to the technical support service.

CODE DESCRIPTION (Sort by description) TU11531 OPERATING ARM TEFLON TUBE
ME14671 2 WAY ELECTROVALVE WITH NUTS FO14717 photodetector autocenter probe (2)
MO11522 60mm FAN PROVE FO11562 PHOTODETECTOR SET (5)
AC14708 A25 LENS SET PC11552 PHOTOMETRIC BOARD
AC11860 adjust temperatura tool PC11556 POWER SUPPLY BOARD
AC13113 ARM CABLES A25 PC14716 POWER SUPPLY instrument
CA11550 ARM HOUSING AC14463 POWER SUPPLY-CPU CABLES
ac11572 BASE HOUSING AC14225 PROBE O-RING
AC14731 CD SERVICE SOFTWARE 83101 with service CE11561 probe peltier cell
manual PC14709 RACKS DETECTION BOARD
PC11862 COMMUNICATION BOARD AC14706 RACKS SUP.TRAY WITH METAL BASE
AC13082 CONECTORS &PINS FOR A25 AC14707 REACTION ROTOR GROUP
TU11533 CONTAINER TUBING SET ac11573 REAR HOUSING
AC13362 DISPENSING PUMP FLUID. CHAMBER AC13079 REFILL CERAMIC PUMP
ME13359 DISPENSING PUMP GROUP ME11543 ROTOR BELT
MO13363 DISPENSING PUMP MOTOR with ceramic AC11545 ROTOR COVER DETECTOR
piston
AC13310 ROTOR GEAR
AC13361 DISPENSING PUMP SEALS KIT
AC13304 ROTOR HOLDER
AC14307 DISPENSING PUMP TEFLON TUBE
MO11544 ROTOR MOTOR
ME14728 electronic cover hinge
CE14757 rotor peltier cell
AC14619 encoder spring
AC11542 ROTOR TEMPERATURE SENSOR
AC11519 ENCODER-SPRING WITH Z MOTOR
AC11574 SET HINGE (PACK 2)
AC14713 EPROM UPDATE 83101
AC13227 SPIRAL SET
BO14729 external botlle in-out coupling
AC14715 TEST BOARD EPROM A25
MO11558 FAN 80 mm
AC11521 THERMISTOR PROBE
ME10490 FAN´S GRILL
AC11520 THERMOSTATED NEEDLE SET
AC11546 FILTER WHEEL BODY
AC14763 TOOL LENS
MO11547 FILTER WHEEL MOTOR
AC11580 UPPER HOUSING
AC14721 fridge cover
AC11538 WASHING STATION COVER
AC14724 fridge cover detector
AC11534 WASHING SYSTEM DIAPHRAGM PUMP
AC14720 fridge fan
AC11536 WASHING SYSTEM FLOW RESTRICTOR
AC14722 fridge hinge
TU11537 WASHING SYSTEM PVC TUBES
AC14723 fridge led
AC11539 WASTE CONTAINER NUT AND CAP
AC14719 fridge O-ring
AC14718 WASTE DIAPHRAGM PUMP
CE14758 FRIDGE PELTIER CELL
AC11559 WINDOW TOP COVER
AC14725 fridge power supply
ME14628 X CARRIAGE BELT
AC14726 fridge temperature sensor
AC14714 X LOOSE PULLEY
PC11555 FRONT INDICATOR BOARD
MO14620 X MOTOR
ZO10407 FUSE HOLDERS PACK
AC14710 X TRACK RAIL WITH 2 SLIDE UNIT
ME11549 HYDRO-PNEUMAT.CYLIND.TOP COVER
AC14213 Y AND Z LOOSE PULLEY
ZO14727 instrument holder
ME11513 Y CARRIAGE BELT
ZO13309 LAMP HOLDER FOR TURBI.SYSTEMS
MO11516 Y MOTOR
CE11540 LOAD CELL
AC14711 Y TRACK RAIL WITH 1 SLIDE UNIT
VA10355 MAINS CONNECTOR F370
ME11514 Z CARRIAGE BELT
IN11557 MAINS SWITCH
AC14712 Z TRACK RAIL WITH 1 SLIDE UNIT
ME14670 Manifold 3 way electrovalve
PC11551 MICROPROCESOR BOARD
PC11553 NEEDLE CONDITIONING BOARD
86
VA10355 ZO10407 ME10490 ME11513 ME11514 MO11516
AC11519 AC11520 AC11521 MO11522 AC3361 TU11531
TU11533 AC11534 AC11536 TU11537 AC11538 AC11539
CE11540 AC11542 ME11543 MO11544 AC11545 AC11546
MO11547 ME11549 AC11550 PC11551 PC11552 PC11553
PC11555 PC11556 IN11557 MO11558 AC11559 CE11561
FO11562 AC11569 AC11572 AC11573 AC11574 AC11580

AC11860 PC11862 AC13227 AC13304 ZO1309 AC13310
87
Service manual
ME13359 AC13362 MO13363 AC14213 AC14225 AC14307
AC14619 MO14620 ME14628 ME14670 ME14671 AC14706
AC14707 AC14708 PC14709 AC14710 AC14711 AC14712
AC14714 PC14716 FO14717 AC14718 AC14719 AC14720
AC14721 AC14722 AC14723 AC14725 AC14726 ZO14727
ME14728 BO14729 AC14731 CE14757 CE14758 AC14763
A IV. LIST OF REQUIRED TOOLS

1. Set of metric Allen keys.
2. Loctite 243 screwfastener or similar
3. Mechanical grease ELESA NT1 (for ceramic pump only)(AC13079).
4. Heat silicone or similar
5. Soldering iron
6. Screwdrivers or two 3 mm Allen keys.
7. Loctite 245 or similar (for ceramic pump only).
8. Assembly tool: Tool set lens key (AC14763)
9. Temperature adjustment tool(AC11860).
10. Adjustment tool for the rotor dispensing point. (AC15920)
88
A V. SOFTWARE VERSIONS
Change in the versions of service program
Date version Change
3/06/03 1.3 First versions
14/04/03 1.3.2 Corrections
4/06/04 2.0.0 Improve the rotor verification test
Improve some display screen
Improve the test/change lamp
New screen: save and load the file adjustments
New initialitation screen. shows the state information of the analyser
09/07/04 2.5.0 Change the sensitivity adjust
20/03/06 3.0.0 New adjustment: adjust the maximum sweep of the z axis
New test of the maximum sweep of the z axis
Improve the level detection test
Improve the photometric test
Improve the stress test
New user’s menu
Improve the read/load adjustment and cycles
28/06/06 3.0.2 Correction of errors
Improve the checking before load a new firmware
30/11/06 3.1.0 Modify the level sensitivity adjustment screen to incorporate the in-
dependent adjustment by type of rack.
Added the help menu with the preventive maintenance guide.
14/12/07 3.2.0 Improve the fine zmaz adjustment. Include a new menu to change
the rotor type.
17/07/09 4.1 Modify the adjustment menu to include an option to select the type
of racks: metal rack or plastic racks.
Included a new menu to use the photometry tool.
19/3/12 4.2 Add the identification of Y25.
Change in the versions of user program

Date version Changes
24/07/03 1.4.0 First version
14/11/03 1.4.1 Corrections and new languages implemented, russian
27/11/03 1.4.2 Corrections
10/02/04 2.0.0 New funtionality: Sat report
Importr/ exportr sesions
PC-host (LIMS) module
Possibility to create different users levels
Add sound alarm
8/07/04 2.5.0 New contamination file
Possibility to modify the factor by the user
USB connection
Use different type of rack with the racks deteccion activate
Change in the rotor verification test
28/01/05 3.0.0 Automatic repetition
Printed automatic final reports
Cancel samples patient and test
Delete blanks and standards
Improve the downcounting time
89
Service manual
Possibility to save test in a file

Improve turbidimetry test
Start running automaticaly after doing a New rotor
New Button in monitor screen shows the preparation/well
Read patient code with barcode reader
Corrections
13/09/05 3.1.1 Calculated test
New programming test options:
Decreasing calibrations curve
Reactive blank with saline solution
New type of sample: Whole blood
20/03/06 3.2.0 Correction of Run Time errors
Improve the Base Line execution (repetion when it fails)
26/06/06 3.2.2 New button in the tools menu, reset the historical base line
Fix the options of the initial and final wash. Delete the menu to cho-
ose the parameter of initial and final wash.
14/11/06 3.3.0 Improve and optimitation of the execution times of the application.
Corrected the application errors that showed the message «not res-
pond»
21/03/07 3.3.2 Added the Korean language. Correction of Run Time errors.
14/06/07 4.0.0 Added the Romanian and indonesian languages
Added the substrate depletion control function in kinetic test
Added the prozone control function in turbidimetric test
Added the correlation of result per test with other analysers
Added graphical representation of results
Change the reagent Blank use in calibration factor calculation
Added a new patient results report
Added a new document explaining the Remarks messages
17/07/09 4.1 New function of LIMS online communication.
New function of the calibration plot: when a concnetration value is out
of the plot it can repeat with a postdilution factor.
27/08/09 4.1.1 Correction to run the calibrator of Hb total test.
14/05/10 4.5 Improved the management of bireagent test with reading time of 900s
New option to share positions in the rack of reagents with different
test.
Displaying the kinetic curve of calibrators.
Add new test in samples with AutoNumber identifier.
1/10/10 4.5.2 Correction of Magnesium Xylidil test. Added new contamination pro-
gramation. Added new calculated test.
1/8/11 4.6 New test to HbA1C.
Change of the type name of the test from PLM to SEM and from CSF
to LIQ.
Shows the regression line and the regression coefficient in the test
with calibration curve and linear regression.
19/3/12 4.7 Possibility of positioning tubes and pediatric wells in the same rack.
New functionality of automatic calibration curve by dilluciones from a
single calibrator.
Change in the versions of firmware

Date version Change
21/05/03 1.0 First version
9/06/03 1.3 Correction of washing cycles. Correction of Base line. Correction of
communications.
90
25/07/03 1.34 Improve of switch on/off of the analyser. Robust rotor manoeuvre.
1/12/03 1.40 Improve the communications of auxiliar channel. Improve the stress
mode. Correction of integration time calculus.
13/02/04 1.60 Improve the information send by the auxiliar channel. Improve the
switch off lamp. Correction of the temperature rotor and probe control.
9/07/04 2.00 Improve and robust the communications. New implementation of
the turbidimetric system. New functional of the alarm acustic signal.
Correction, unification of the criterions of service and user programs.
Improve the stress mode. Add the sensitivity map of the racks.
23/07/04 2.02 Corrections of the level deteccion map.
28/02/05 2.40 Improve the demo test. Improve the bireactive test with contaminations.
Enlarge the integration time limits. Improve the encoder control.
4/03/05 2.42
14/03/05 2.44 Corretion of the synthesis of FPGA
17/05/05 2.46 Add 2 new type of racks of 13 and 15mm. Correction of stress test.
31/05/05 2.48 Correction of encoder control
14/09/05 2.50 Impove, from user program load the analyser adjustments.
03/02/06 2.54 Correction of the encoder error during warming-up and shut-down
20/03/06 2.80 Improve the execution of base line
new adjustment, the maximum sweep of the z axis
New cycles meter
Correct the bireactive, bichromatic with contamination test
26/05/06 2.94 Increase the acceptance range of base line check
Increase the Z motor current to 1.6A
30/11/06 3.00 In order to avoid the splashes in the dispensation of the predilution,
the dispensation speed have been reduced.
The sensitivity adjustment have been split in 3 diferents adjustments,
one for each type of rack.
04/04/07 3.15 In order to avoid a rotor overheating, this version has a protection in
the rotor thermo. This only occur when an error appear during the
analyser initialitation.
20/07/07 3.22 Included a autodiagnostic tool.
Correction during global stress of the filter wheel lost steps
Correction of the reading system. During a bicromatic test happens a
error of encoder, then there are some erroneous readings.
17/07/09 3.60 Correction about enter the serial number of the analyzer
Added a protection of the Shut-down process.
9/11/09 3.64 Increase of Z motor current to 1.6A. Improve the movement of Z
motor.
17/05/10 3.68 Improve the dispensing maneuver.
1/06/10 3.72 Improve in the predilution maneuver.
Improve in the bireagent maneuver with reading time of 900s
28/06/10 3.73 Fixed a bug of level detection with metallic racks when the CPU is
mounted in A25 with fridge.
31/08/10 3.74 Fixed a bug of level detection with 13/15mm racks in the position 4,
5 and 6 of the tray rack, when the CPU is mounted in A25 with frid-
ge.
18/10/10 3.75 Fixed a bug in the detection of board CIIM00010-06
10/3/10 3.77 Improve the dispensing manoubre.
91
Service manual
Compatibilities table A25
User A25 3.0.0 3.1.1 3.2.0 3.2.2 3.3.0 3.3.2 4.0.0 4.0.8 4.1 4.5 4.5.2 4.6 4.7
firmware
2.40 x x x x x x x x x
2.50 x x x x x x x x
3.72 x x x x
3.73 x x x x
3.74 x x x x
3.75 x x x x
3.77 x x x x
It is advisable to always install the last existing version of firmware.
Change in the versions of user program

Date version Changes
19/3/12 4.7 Possibility of positioning tubes and pediatric wells in the same rack.
New functionality of automatic calibration curve by dilluciones from a
single calibrator.
Compatibilities table Y25

User Y25 4.7
firmware
3.77 x
It is advisable to always install the last existing version of firmware.
92
A VI. REFURBISH INSTRUCTIONS
This chapter explains the instructions for the refurbish and control of the instruments traded as rental or loan, or
second hand.
Terms
• Life: Analyzers have the functionality for life as long as they keep maintenance conditions specified by the ma-
nufacturer and the use of original spare parts. Note that the manufacturer is obliged to provide spare parts up to
5 years after discontinuing the analyzer.
• Number of times can be refurbished: You can refurbish as many times as necessary, provided the overhaul
following the instructions described in this section and the use of original spare parts.
• Accessories used in refurbished devices: Any accessories used in refurbished analyzer are provided by the
manufacturer. Any fungible remaining from the last use such as rotors, extra reagent bottles, cleaning solution,
fluid system, and so on, will discarded.
• Staff: The refurbishment will be done by personnel authorized by Biosystems. It will normally be the distributor
service staff, or someone who has received the appropriate training.
Instructions
Cleaning and disinfection
Wear gloves and protection for the user who will perform the disinfection process. Treat disposables (rotors, reagent
bottles, sample tubes) as potentially infectious.
Check if there is any reaction rotor in the housing and reagent bottles. If so, discard them.
Empty waste containers and liquid system.
Clean the outside of the computer with a damp cloth and mild soap. In case of splash, locally wipe with alcohol.
Follow the current national regulations for disposal for potentially infectious waste.
Components to change
• Change the tip. (AC11500) Use the utility change the tip of the service program. Discard the tip. Treat as potentially
infectious. To manipulate the tip wear gloves to protect the user.
• Changing the inner tubes of Teflon (AC11569)
• Changing the lamp and he lamp socket. (ZO13309) Use the utility lamp replacement of the service program.
• Changing the pump seal ceramic (AC13361)
• Update firmware and software
Adjustment Process
Register values obtained from the adjustment in the record document provided at the end of the service manual.
Make the following settings, consult the manual (Chapter 3) how to make the adjustments.
• Self-centering tip
• XY position adjustment
• XYZ Adjusting tray positions
• Position of filters wheel
• Dispensing of position point
• Rotor position in optics
• Sensitivity level detection
• Thermostatting tip
• Thermostatting rotor
• Photometry test
• Integration times
• Number of accounts
• Accounts of darkness
• Repeatability without moving filters wheel
93
Service manual
• Stability at 505 nm
• Repeatability moving filters
Final functional verification
Run with the user program.
For functional verification use the following reagents and reference materials from Biosystems:
Glucose reagent(12 503)
ALT reagent (12 533)
Biochemistry calibrator (18 011)
Biochemistry Control Serum level I and II (18009 and 18010)
Modify the following parameters of the programming test of glucose and ALT:
Number of replicates: 20
Concentration of calibrator: enter the value of the insert
Set the following list:

Two patients called Level I and Level II
In each patient, program test: Glucose and ALT
Run the list. The analyzer will perform the blank, calibrator and 20 replicates of each level of control serum. Once
finished, the results will be displayed.
With the 20 replicated for result, calculate the CV.
The CV is calculated as: CV = des. Std / mean * 100
Verify that the results are within the ranges

Precision biochemistry CV(%)
Control serum level I glucose <2
Control serum level II glucose <2
Control serum level I ALT <4
Cotrol serum level II ALT <2
Verify that the average results for each control serum are within the ranges indicated in the insert of each control serum.
If the results do not fall within the above ranges, the analyzer is damaged. Repair the analyzer. Once repaired perform
again functional verification.
Ending
• Place an indelible label to the refurbished analyzer. The label contains the following information (check with the
national legislation of each country):
1. The name and address of the company responsible for implementing and refurbishing
2. The date of last revision
3. The number of refurbish performed
4. Indication that it is a renewed equipment
5. Lifetime after refurbishing.
• Add a new box of accessories (AC16064).
• Use original packaging. Previously check it the status and if it is deteriorated then replace by a new one.
• Send completed record to the Bioystems service technician (pdf) sat@biosystems.es
94
Manufactured by: BioSystems, S.A.
Costa Brava, 30, 08030 Barcelona - Spain Tel: 34-93 311 00 00 Fax: 34-93 346 77 99
e-mail: biosystems@biosystems.es http://www.biosystems-sa.com
RECORD OF REFURBISH PROCESS
Company name: Z 459 414

Serial number Z relation Pedi. - Tube 270 310
Refurbish date: Reagnet rack
R1 p1 X 480 520
SPARE CHANGE PROCESS Y 30 70
Z 745 695
1. Spare change R1 p10 X 480 520
TIp change Ok / No Ok Y 1370 1410
Teflon tubing change Ok / No Ok Z 745 695
Lamp change Ok / No Ok R2 p1 X 810 850
Pump seal change Ok / No Ok Y 30 70
Update firmware and Z 745 695
Ok / No Ok
software R2 p10 X 810 850
Y 1370 1410
ADJUSTMENT PROCESS Z 745 695
R3 p1 X 1140 1180
Adjustments steps Tolerances Value Y 30 70
2. Autocentering tip Z 745 695
Offset X -4 4 R3 p10 X 1140 1180
Offset Y -4 4 Y 1370 1410
3. XY positions adjustment Z 745 695
X origin 5 130 Fridge reagent rack
Y origin 0 45 R1 p1 X 1685 1725
X washing station 1325 1360 Y 30 70
Y washing station 0 35 Z 747 713
X Rotor 2855 2895 R1 p10 X 1685 1725
Y Rotor 650 690 Y 1295 1335
4. XYZ rack adjustment Z 747 713
Pediatric and tube racks R2 p1 X 1985 2025
R1 p1 X 320 360 Y 30 70
Y 20 60 Z 747 713
Z 459 414 R2 p10 X 1985 2025
R1 p12 X 320 360 Y 1295 1335
Y 1375 1415 Z 747 713
Z 459 414 R3 p1 X 2275 2315
R2 p1 X 660 700 Y 30 70
Y 20 60 Z 747 713
Z 459 414 R3 p10 X 2275 2315
R2 p12 X 660 700 Y 1295 1335
Y 1375 1415 Z 747 713
Z 459 414 5. Filter wheel adjustment
R3 p1 X 990 1030 Position -6 0
Y 20 60 6. Centering Rotor regard of dispensing point
Z 459 414 Rotor position -10 30
R3 p12 X 990 1030 X fine of rotor 2860 2897
Y 1375 1415 7. Centering Rotor regard of optical system
Page 1/2 Rev 1.0 Operative date: 07-09-11

RECORD OF REFURBISH PROCESS
Position -12 6 F8 670 nm 3700 4300

8. Level detection sensitivity 11.3. Repeatability SMF Tolerancias
Pediatric rack 45 120 Desv. Est. Abs. F1 340 nm ≤0.0004
Tube rack 25 100 Desv. Est. Abs. F2 405 nm ≤0.0006
Reagent rack 10 60 Desv.Est.Abs.F3 505 nm ≤0.0004
Fridge reagent rack 10 60 Desv.Est.Abs.F8 670 nm ≤0.0007
9. Tip thermostatation 11.4. Stability to 505 nm
Set point temperature 35,0 45,0 St. Dev. Abs.F3 505 nm ≤0.0008
Temp. Dispens. 1 36,0 37,5 Max. number of counts 840000 940000
Temp. Dispens. 2 36,3 37,5 Min. number of counts 840000 940000
Temp. Dispens. 3 36,5 37,5 Max NC / min. NC ≤1.006
Temp. Dispens. 4 36,5 37,5 11.5. Repeatability
Temp. Dispens. 5 36,5 37,5 St. Dev. Abs.F1 340 nm ≤0.0008
Temp. Dispens. 6 36,5 37,5 St. Dev. Abs.F2 405 nm ≤0.0006
10. Rotor thermostatation St. Dev. Abs.F3 505 nm ≤0.0005
Set poin ttemperature 36,5 38,0 St. Dev. Abs.F4 535 nm ≤0.0005
Temp. sector well 1 36,0 37,5 St. Dev. Abs.F5 560 nm ≤0.0005
Temp. sector well 5 36,5 37,5
Temp. sector well 6 36,5 37,5 FINAL FUNCTIONAL VERIFICATION
Temp. sector well 7 36,5 37,5
Temp. sector well 8 36,5 37,5 12. Precision biochemistry Tolerance CV(%) Value
Mean temperature [2-8] 36,8 37,2 Control serum level I glu-
<2
11. Photometry tests cose
11.1. Base Line Control serum level II glu-
<2
cose
11.1.1. Integration time
Control serum level I ALT <4
F1 340 nm 30,0 70,0
Control serum level II ALT <2
F2 405 nm 6,0 26,0
F3 505 nm 6,0 26,0
TERMINATION
F4 535 nm 6,0 26,0
F5 560 nm 6,0 26,0
13. Finish
F6 600 nm 6,0 26,0
Place label of refurbished Ok / No Ok
F7 635 nm 6,0 26,0
equipment
F8 670 nm 6,0 26,0
Add full box of accesories Ok / No Ok
TI Ref Save SI
Check packaging Ok / No Ok
11.1.2. Number of counts
F1 340 nm 750000 960000
14. Acceptance of refurbished
F2 405 nm 840000 940000
F3 505 nm 840000 940000 Refurbished OK
Ok / No Ok
Available for sale
F8 670 nm 840000 940000
11.2. Darkness counts
F1 340 nm 3700 4300
F2 405 nm 3700 4300
F3 505 nm 3700 4300
Page 2/2 Rev 1.0 Operative date: 07-09-11

A25 Service Manual - English

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A25 - Y25

2. Electronic system and fluids............................................. 13

3.3.2.2. Stress mode test............................................................................................................................... 52

1.1. GENERAL DESCRIPTION OF THE ANALYZER

1.1.1. Operating arm

1.1.2. Dispensing system

1.1.3. Reactions rotor and reading

1.1.4. Electronic system

1.1.5. Application program

1.2. FUNCTIONING OF THE ANALYZER

1.3. TRANSPORT AND RESHIPMENT OF THE ANALYZER

RANDOM ACCESS ANALYZER A25

To free the lid and it can open and

If it becomes necessary to return the analyzer, or transport it by vehicle, it is important

Description of the electronics of the A25 analyzer.

There are 8 different boards, which correspond to:

CPU Board (CIIM00006)

2.1 CPU Board (CIIM00006)

Connector Function Pins

Connector Function Pins

J32 Y axis home 1 - Home

The board has an acoustic alarm.

Item Cables PC15061 board

The connectors J9, J10 and J11 are unconnected.

2.2 Power Supply Board (CIIM00007)

Connector Function Pins

J1 5 V output and enabling of power 1 - 5V

J8 Connection to I2C bus with the CPU 1- 5V

J4 36V and 12 V output voltage. Su- 1 - 36V

J8 Connection to I2C bus to connect with 1- 5V

TP36V - 36V voltage

List of LED diodes:

2.3 Needle Board (CIIM00008)

Connector Function Pins

J1 CPU board connection (CIIM00006) 1 - Fan control

J2 CPU board connection (CIIM00006) for the Peltier 1 - Peltier

J4 Peltier 1 - Peltier, red

J5 Fan 1 - Fan control

J6 Needle connection for level detection 1 - needle

TP1 - Needle signal

2.4 Photometry Board (CIIM00009)

Connector Function Pins

J3 Photometric board connection (CIIM00009) 1 - 12 V

2.5 Racks Board (CIIM00010)

Connector Function Pins

2.6 LED Board (CIIM00011)

Connector Function Pins

J36 LED board connection (CIIM000011) 1 - GND

CN1 - USB Connector

D1 - USB TX LED indicator

Connector Function Pins

J1 CPU board connection (CIIM0006) 1- Vcc

2.8 List of components

2.9 Auxiliar channel information

there are two connection types:

Only connect one cable type.

The labeled connector COM2 is the auxiliar connector.

Baud Rate: 38400

BioSystems S.A. 2007

BIOSYSTEMS A25 [FRIDGE]

3.3.1.5. Z axis security systems test