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    Xii Chemistry Practical.pdf

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    neersharma.professor
    Important part of class 12

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    Xii Chemistry Practical.pdf

    Uploaded by

    neersharma.professor
    7 views2 pages
    Important part of class 12

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    © © All Rights Reserved

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    PDF, TXT or read online from Scribd

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    Important part of class 12

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    7 views2 pages

    Xii Chemistry Practical.pdf

    Uploaded by

    neersharma.professor
    Important part of class 12

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 2

    b- Confirmatory test :

    (i) Sodium Nitroprousside Test :


    O
    ||
    CH 3  C  CH 3  OH (  ) 
     CH 3COCH 2(  )  H 2O

    3
    O  O 
    ||  || 
     
    Fe(CN ) NO   CH 3

    5
    2
    C  C H2 --  Fe CN 5 NO CH 3  C  CH 2

     
     
     
    Result : Given organic compound contains Ketonic group (>C = 0)

    EXPERIMENT – 22
    Aim : To study carbohydrates in pure form of detect its presence in food.
    Appeartus required : Test tube, test tube stand, test tube holder, burner etc.
    Theory : Carbohydrates are Polyhydroxy aldehydes or polyhydroxy ketones, their derivatives and the substance
    which yield them on hydrolysis carbohydrates are classified as sugars and non sugars. Sugars like glucose, fructose
    and cane sugar are crystalline. Among sugars, glucose, fructose and lactose are reducing while sucrose is non reducing
    sugar.
    Test :
    Experiment Observation Inference
    1 Conc. H2SO4 test: Charring with Carbohydrates present.
    Sample + burnt sugar
    Conc. H2SO4 + Heat smell.
    2 Molisch test : Reddish purple Carbohydrates present.
    Aq. Solution of sample + 2 ring in formed
    drops of 1%  naphthol  at the junction
    + conc. H2SO4. of 2 layers
    3 Fehling solution test : Red ppt Reading
    Sample solution + 1 ml of Sugar persent
    fehling A + 1 ml of Fehling B
    +
    4 Tollens reagent : Silver mirror is Reducing sugar present.
    Aq. Solution of sample + 2 formed along
    ml of Tollens eagent +  the surface of
    test tube.
    5 Benedit’s Solution test: Red ppt Reducing sugar present
    Aq solution of sample +
    Benedict’s reagent + Heat
    6 Iodine test : Violet colour Starch is present.
    Sample Solution + few drops
    of iodine solution
    Result : Carbohydrates is present.

    EXPERIMENT – 23
    Aim : To study fat in pure form and to detect into presence in given food sample.
    Apparatus required : Test tube stand, test tube holder, burner etc.
    Theory : Fats and oil are esters of long chain fatty acids and glycerol and thus also called glycerides. Fats contains
    saturated fatty acids while oil contain unsaturated fatty acids.
    Test :
    S.no. Experiment Observation Inference
    1 Spot Test : Put a small amount of Translucent spot Fat is present.
    (CHEMISTRY PRACTICALS) Page No 25
    sample on a filter paper and press appears on the filter
    with another filter paper . paper
    2 Acrolein test : Irritating smell Fat is present
    Take a few drops of sample in a appears due to the
    test tube. Add few drops of form of acrolein
    potassium bisulphite to it and vapours.
    Heat.
    3 Solubility test : Sample does not
    Take a small amount of sample in dissolve in H2O but is
    3 test tubes. Add water, alcohol soluble in alcohol on
    and chloroform in 1, 2, 3 test heating and soluble Fat is present
    tube respectively. in chloroform.
    Result : Fat is present.

    EXPERIMENT – 24

    Aim : To study proteins from sample and detect their presence in food sample.

    Apparatus required : Test tube stand, test tube holder, glass rod, burner etc.

    Theory : Proteins are high molecular mass, long chain polymers composed of amino acid. Amino acids are
    molecule that have both -NH2 and –COOH group.
    Test :
    S.no. Experiment Observation Inference
    1. Biuret test : Sample + NaOH + Bluish violet colour Protein is present
    Dil CuSO4 solution appears
    2. Xanthoprotein test : Yellow ppt. Protein is present
    Sample + few drops of
    conc.HNO3 + 
    3. Million’s test : White ppt Protein is present
    Sample + 2 drops of millions which changes to
    reagent +  brick red on boiling
    4. Ninhydrin test : Blue colour appear Protein is present
    Protein sample + Few drops of
    ninhydrin solution + Boil the
    contents for 1 minute

    Result : Protein is present.

    (CHEMISTRY PRACTICALS) Page No 26

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