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    Cyclic Voltametry For The Detection of Dopamine in Vivo

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    Mahmoud AbdAllah
    This document discusses using fast-scan cyclic voltammetry (FSCV) with carbon fiber microelectrodes to detect dopamine in vivo. FSCV applies a high scan rate to detect dopamine on a millisecond timescale. Carbon fiber microelectrodes are implanted to detect dopamine released from dopaminergic neurons. FSCV can detect evoked and spontaneous dopamine release in the brains of living rats and shows how cocaine increases dopamine signaling.

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    Cyclic Voltametry For The Detection of Dopamine in Vivo

    Uploaded by

    Mahmoud AbdAllah
    81 views12 pages
    This document discusses using fast-scan cyclic voltammetry (FSCV) with carbon fiber microelectrodes to detect dopamine in vivo. FSCV applies a high scan rate to detect dopamine on a millisecond timescale. Carbon fiber microelectrodes are implanted to detect dopamine released from dopaminergic neurons. FSCV can detect evoked and spontaneous dopamine release in the brains of living rats and shows how cocaine increases dopamine signaling.

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    cyclic volt

    Original Title

    Cyclic Voltammetry Final

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    This document discusses using fast-scan cyclic voltammetry (FSCV) with carbon fiber microelectrodes to detect dopamine in vivo. FSCV applies a high scan rate to detect dopamine on a millisecond timescale. Carbon fiber microelectrodes are implanted to detect dopamine released from dopaminergic neurons. FSCV can detect evoked and spontaneous dopamine release in the brains of living rats and shows how cocaine increases dopamine signaling.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    81 views12 pages

    Cyclic Voltametry For The Detection of Dopamine in Vivo

    Uploaded by

    Mahmoud AbdAllah
    This document discusses using fast-scan cyclic voltammetry (FSCV) with carbon fiber microelectrodes to detect dopamine in vivo. FSCV applies a high scan rate to detect dopamine on a millisecond timescale. Carbon fiber microelectrodes are implanted to detect dopamine released from dopaminergic neurons. FSCV can detect evoked and spontaneous dopamine release in the brains of living rats and shows how cocaine increases dopamine signaling.

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    Cyclic Voltametry for the

    Detection of Dopamine in vivo


    Dopamine
    • Neurotransmitter
    – small molecule chemical messenger
    • Important for motor and
    cognitive functions
    – Deficts in dopamine levels cause
    Parkinson Disease
    • Regulates reward Structure of Dopamine.
    4-(2-aminoethyl)
    – Dopamine increases after drugs of benzene-1,2-diol
    abuse like cocaine
    Dopaminergic Neurons
    • Dopamine is synthesized in dopaminergic neurons
    and packaged into membrane bound vesicles
    • Electrical action potential initiates the release of
    dopamine
    • Dopamine vesicles undergo exocytosis
    – Spills out into the extracellular space
    – Can be detected

    The dompaminergic neuron can


    release dopamine into the
    extracellular space where its
    contents can be detected by
    target neurons.
    Detection of Dopamine
    • Exocytosis of dopamine from vesicle occurs on
    a millisecond time scale
    • Sensor must be fast, sensitive, and selective
    since dopamine concentrations are low
    • Fast scan cyclic voltametry is the dominant
    electrochemical technique used
    Dopamine Dopamine-o-quinone
    HO NH2 - O NH2
    -2e
    + 2 H+
    HO O
    Carbon Fiber Microelectrodes
    • Carbon Fiber
    Microelectrodes (CFME)
    are electrodes in which a
    carbon fibers serve as the
    electroactive area
    • Advantages:
    – Carbon fibers are biological
    compatible to cells
    – Small size (less than 10um
    in diameters) allows for Carbon Fiber Microelectrode
    implantation in vivo
    • Commonly used with cyclic
    voltametry
    Voltammetry
    • Electrochemical technique in which the
    current (I) is measured as a function of voltage
    (Eapp).
    • There are several kinds:
    – Linear Scan voltametry (Polarography)
    – Differential pulse polarography
    – Square-wave voltametry
    – Cyclic Voltametry
    Fast-scan Cyclic Voltametry
    1.3 V
    400 V/s

    -0.4 V
    8.5 ms 100 ms

    Scan electrode from a holding potential to a switching potential and back at a


    high scan rate. Repeat these scans every 100 ms.

    Dopamine Dopamine-o-quinone
    HO NH2 - O NH2
    -2e
    + 2 H+
    HO O

    As potential is ramped up, dopamine is oxidized to dopamine-o-quinone.


    As potential is ramped down, dopamine-o-quinone reduced back to dopamine.
    Fast-Scan Cyclic Voltametry
    Cyclic voltammograms Background-subtracted
    No dopamine
    Dopamine present
    cyclic voltammogram of dopamine
    oxidation

    reduction

    Fast scan rate cause a large background The background current is stable and can
    charging current due to charging be subtracted out to obtain a background-
    the double layer. subtracted cyclic voltammogram for dopamine.

    When dopamine is added (red line), The position of the peaks help identify the
    the differences are small. molecule being detected.
    Fast-scan cyclic voltametry
    10ss
    10
    -0.4VV
    -0.4
    dopamine
    reduction 33
    Ag/AgCl
    E EvsvsAg/AgCl

    00
    1.0VV
    1.0 i, nA

    -5
    -5
    dopamine i, nA
    oxidation
    -0.4VV
    -0.4
    dopamine present

    •Data can also be depicted as a color plot to show many CVs over time.
    •Current is in color and shows when dopamine is present.
    Results
    Electrically-Stimulated release in a rat

    •Carbon-fiber microelectrode implanted in nucleus accumbens


    •Nucleus accumbens regulates reward
    •Electrically stimulate cell bodies in ventral tegmental area
    •Size of dopamine evoked depends on frequency of stimulation
    • Color plots look like dopamine
    Results
    Spontaneous dopamine transients in a rat

    Spontaneous dopamine transients in a rat after cocaine

    •Cocaine increases the concentration and the length of time for dopamine
    signaling.
    Advantages
    • Allows real-time detection of dopamine in
    behaving animals
    • Can determine during what behaviors
    dopamine is released
    • Can determine how drugs affect dopamine
    signaling in the brain

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