PLANT PHYSIOLOGY

1. PENDAHULUAN
2. REAKSI TERANG
FOTOSINTESIS
3. REAKSI GELAP
FOTOSINTESIS
4. EKOLOGI
FOTOSINTESIS
5. TRANSLOKASI
KARBOHIDRAT
6. RESPIRASI
 Aturan Perkuliahan
 Datang tepat waktu
 Dilarang titip absen, jika berhalangan hadir
harus ada surat ijin yang jelas
 Jika absensi kurang dari 80% maka
dilarang mengikuti UAS
 Tidak ada tugas tambahan untuk
perbaikan nilai akhir
 TUGAS TERSTRUKTUR
Paper dan Presentasi dengan topik :
 Reaksi Cahaya (Terang)
 Reaksi Gelap
 Fotosintesis C3, C4, CAM
 Ekologi Fotosintesis
 Translokasi karbohidrat
 Respirasi

Paper min 10 halaman (Sampul, abstrak, pendahuluan,

pembahasan, penutup, daftar pustaka)
Pustaka min 3 Artikel Penelitian, 3 Text Book
 OBJECTIVE
 To understand and uncover the law of life
activity of plant
 To protect and utilize plant in the
agricultural and industrial practice
according to the law
 LECTURE LAYOUT
1. INTRODUCTION 2. ENERGY
1. Definition 1. Definition
2. History Of Plant 2. Energy Of Organisms
Physiology 3. Law of Conservation of
3. How Plants Work Energy
4. Syllabus 4. State and Form of Energy
References 5. Electron Energy
6. Free Energy
 1.1 Definition
What is Plant Physiology?

1. Plant physiology is the study of

 the functions and processes occurring in plants
 the vital processes occurring in plants
 how plants work

2. Plant physiology is the study of plant

function encompassing
 the dynamic processes of growth
 metabolism and
 reproduction in living plant (Taiz L and Zeiger E
1991).
3. Plant physiology is a study of the plant way of life,
which include various aspects of the plant lifestyle
and survival including:
 metabolism,
 water relations,
 mineral nutrition,
 development,
 movement,
 irritability (response to the environment),
 organization,
 growth, and
 transport processes
INTRODUCTION

What is Plant Physiology about ?

4. Plant physiology is about

 how plants use the energy of sun to assimilate
carbon,
 how they convert that carbon to stuff of which
they are made,
 how plants obtain and distribute nutrients and
water,
 how they grow and develop,
 how they respond to their environment,
 how they react to stress,
 how they reproduce
In short, plant physiology is about HOW PLANTS
WORK
 1.2 History of Plant Physiology
1. Sir Francis Bacon (1561 –1626), an English
philosopher, published one of the first plant
physiology experiments in 1627 in the book, Sylva
Sylvarum.
Bacon grew several terrestrial
plants, including a rose, in water
and concluded that soil was only
needed to keep the plant upright
2. Jan van Helmont
began the research of the process in the mid-
1600s when he carefully measured the mass of
the soil used by a plant and the mass of the
plant as it grew.

After noticing that the soil

mass changed very little, he
hypothesized that the mass of
the growing plant must come
from the water, the only
substance he added to the
potted plant. .
3. John Woodward
(1 May 1665 – 25 April 1728), an
English naturalist, antiquarian and
geologist, published experiments
in 1699 on growth of spearmint
(Mentha spicata) in different
sources of water.
He found that plants grew much
better in water with soil added
than in distilled water.
4. Stephen Hales, FRS (17 September
1677 – 4 January 1761), an English
physiologist, chemist and inventor

He is considered the Father of Plant Physiology for

the many experiments in the 1727.
Hales studied the role of air and water in the
maintenance of both plant and animal life.
5. Joseph PriestleyO2
a chemist and minister, discovered that when he isolated a
volume of air under an inverted jar, and burned a candle in it, the
candle would burn out very quickly, much before it ran out of
wax. He further discovered that a mouse could similarly "injure"
air. He then showed that the air that had been "injured" by the
candle and the mouse could be restored by a plant.
6. Jan Ingenhousz O2 produce d by plants +light
a court physician to the Austrian Empress, repeated Priestley's
experiments in 1778, . He discovered that it was the influence of
sun and light on the plant that could cause it to rescue a mouse
in a matter of hours.
7. Jean Senebier CO2 taken up by plants
a French pastor, showed in 1796, that CO2 was the "fixed" or
"injured" air and that it was taken up by plants in
photosynthesis.
8. Nicolas-Théodore de Saussure CO2 +H2O
soon afterwards, showed that the increase in mass of the
plant as it grows could not be due only to uptake of CO2,
but also to the incorporation of water. Thus the basic
reaction by which photosynthesis is used to produce food
(such as glucose) was outlined.

Modern scientists built on the foundation of knowledge

from those scientists centuries ago and were able to
discover many things
9. Cornelius Van Niel  Chemistry of photosynthesis
made key discoveries explaining the chemistry of
photosynthesis. By studying purple sulfur bacteria and
green bacteria, he was the first scientist to demonstrate
that photosynthesis is a light-dependent redox reaction, in
which hydrogen reduces carbon dioxide.
10. Robert Hill O2 (photosynthesis) H2O
He in 1937 and 1939 performed further experiments to
prove that the oxygen developed during the
photosynthesis of green plants came from water
He showed that isolated chloroplasts give off oxygen in
the presence of unnatural reducing agents like iron
oxalate, ferricyanide or benzoquinone after exposure
to light.

The Hill reaction is as follows:

 2 H2O + 2 A + (light, chloroplasts) → 2 AH2 + O2

where A is the electron acceptor. Therefore, in light the

electron acceptor is reduced and oxygen is evolved.
11. Samuel Ruben and Martin Camen O2 (phot)
H2O
They used radioactive isotopes to determine that
the oxygen liberated in photosynthesis came
from the water.
12. Melvin Calvin and his partner Benson were able
to puzzle out each stage in the dark or light-
independent phase of photosynthesis, known as
the Calvin Cycle.
13. Rudolph A. Marcus, a Nobel Prize winning
scientist, was able to discover the function and
significance of the electron transport chain.
 1.3 How Plants Work
 Plant Level 6. Christae 18. Golgi body
 Shoot 7. Nucleus 19. Peroxisomes
 Leaves 8. Nuclear membrane 20. Microfilaments
 Stem 9. Nucleolus 21. Microtubules
 Reproductive Organ
10. Centrosome 22. Plasmodesmata
 Root
11. Ribosome 23. Rough
  Cellular Level 12. Chloroplast endoplasmic
1. Cell wall 13. Chlorophyll reticulum
2. Cell membrane 14. Stroma 24. Smooth
3. Cytoplasm endoplasmic
15. Thylakoid disk
4. Vacuole reticulum
16. Granum
5. Mitochondrion 17. Amyloplast
Plant Level
• The Shoot System
• Above ground (usually)
• Elevates the plant above the
soil
• Many functions including:
• photosynthesis
• reproduction & dispersal
• food and water conduction
• The Root System
• Underground (usually)
• Anchor the plant in the soil
• Absorb water and nutrients
• Conduct water and nutrients
• Food Storage
Cellular Level
1. Cell wall - a thick, rigid membrane that surrounds a plant cell. This
layer of cellulose fiber gives the cell most of its support and structure.
The cell wall also bonds with other cell walls to form the structure of
the plant.
2. Cell membrane - the thin layer of protein and fat that surrounds the
cell, but is inside the cell wall. The cell membrane is semipermeable,
allowing some substances to pass into the cell and blocking others.
3. Cytoplasm - the jellylike material outside the cell nucleus in which the
organelles are located.
4. Vacuole - a large, membrane-bound space within a plant cell that is
filled with fluid. Most plant cells have a single vacuole that takes up
much of the cell. It helps maintain the shape of the cell.
5. Mitochondrion - spherical to rod-shaped organelles with a double
membrane. The inner membrane is infolded many times, forming a
series of projections (called cristae). The mitochondrion converts the
energy stored in glucose into ATP (adenosine triphosphate) for the
cell.
6. Christae - (singular crista) the multiply-folded inner membrane of a
cell's mitochondrion that are finger-like projections. The walls of the
cristae are the site of the cell's energy production (it is where ATP is
generated).
7. Nucleus - spherical body containing many organelles, including the
nucleolus. The nucleus controls many of the functions of the cell (by
controlling protein synthesis) and contains DNA (in chromosomes).
The nucleus is surrounded by the nuclear membrane
8. Nuclear membrane - the membrane that surrounds the nucleus.
9. Nucleolus - an organelle within the nucleus - it is where ribosomal RNA
is produced.
10. Centrosome - (also called the "microtubule organizing center") a small
body located near the nucleus - it has a dense center and radiating
tubules. The centrosomes is where microtubules are made. During cell
division (mitosis), the centrosome divides and the two parts move to
opposite sides of the dividing cell. Unlike the centrosomes in animal
cells, plant cell centrosomes do not have centrioles.
11. Ribosome - small organelles composed of RNA-rich cytoplasmic
granules , approximately 60 percent RNA and 40 percent protein , that
are sites of protein synthesis. In eukaryotes, ribosomes are made of four
strands of RNA. In prokaryotes, they consist of three strands of RNA.
12. Chloroplast - an elongated or disc-shaped organelle containing
chlorophyll. Photosynthesis (in which energy from sunlight is converted
into chemical energy - food) takes place in the chloroplasts.
13. Chlorophyll - chlorophyll is a molecule that can use light energy from
sunlight to turn water and carbon dioxide gas into sugar and oxygen
(this process is called photosynthesis). Chlorophyll is magnesium based
and is usually green.
14. Stroma - part of the chloroplasts in plant cells, located within the inner
membrane of chloroplasts, between the grana.
15. Thylakoid disk - thylakoid disks are disk-shaped membrane structures
in chloroplasts that contain chlorophyll. Chloroplasts are made up of
stacks of thylakoid disks; a stack of thylakoid disks is called a granum.
Photosynthesis (the production of ATP molecules from sunlight) takes
place on thylakoid disks.
16. Granum - (plural grana) A stack of thylakoid disks within the chloroplast is
called a granum.
17. Amyloplast - an organelle in some plant cells that stores starch.
Amyloplasts are found in starchy plants like tubers and fruits.
18. Golgi body - (also called the golgi apparatus or golgi complex) a flattened,
layered, sac-like organelle that looks like a stack of pancakes and is located
near the nucleus. The golgi body packages proteins and carbohydrates into
membrane-bound vesicles for "export" from the cell.
19. Peroxisomes - Microbodies are a diverse group of organelles that are found
in the cytoplasm, roughly spherical and bound by a single membrane.
There are several types of microbodies but peroxisomes are the most
common.
20. Microfilaments - Microfilaments are solid rods made of globular proteins
called actin. These filaments are primarily structural in function and are an
important component of the cytoskeleton.
21. Microtubules - These straight, hollow cylinders are found throughout the
cytoplasm of all eukaryotic cells (prokaryotes don't have them) and carry
out a variety of functions, ranging from transport to structural support.
22. Plasmodesmata - Plasmodesmata are small tubes that connect plant
cells to each other, providing living bridges between cells.
23. Rough endoplasmic reticulum - (rough ER) a vast system of
interconnected, membranous, infolded and convoluted sacks that are
located in the cell's cytoplasm (the ER is continuous with the outer
nuclear membrane). Rough ER is covered with ribosomes that give it a
rough appearance. Rough ER transport materials through the cell and
produces proteins in sacks called cisternae (which are sent to the Golgi
body, or inserted into the cell membrane).
24. Smooth endoplasmic reticulum - (smooth ER) a vast system of
interconnected, membranous, infolded and convoluted tubes that are
located in the cell's cytoplasm (the ER is continuous with the outer
nuclear membrane). The space within the ER is called the ER lumen.
Smooth ER transport materials through the cell. It contains enzymes
and produces and digests lipids (fats) and membrane proteins; smooth
ER buds off from rough ER, moving the newly-made proteins and lipids
to the Golgi body and membranes
 1.4 SYLLABUS
LECTURE TOPICS CONTENTS
Introduction
1. ENERGY
Energy
2. PHOTOSYNTESIS I Light Reaction: Synthesis of NADPH

3. PHOTOSYNTESIS II Light Reaction: Synthesis of ATP

4. PHOTOSYNTESIS III Dark Reaction: C3 Plants

5. PHOTOSYNTESIS IV Dark Reaction: C4 & CAM Plants

Glycolysis, TCA Cycle & Terminal

6. RESPIRATION
Oxidation
7. TRANSPORT SYSTEM Phloem and Xylem Transport
 References
1. Taiz, L. and Zeiger, E., 1991. Plant Physiology. The
Benjamin/Cummings Publishing Co., Inc., Redwood City, California
2. Salisbury, F.B. and C.W. Ross. 1992. Plant Physiology
3. Salisbury, F.B. and Ross, C., 1969. Plant Physiology. Wadsworth
Publishing Co. Inc., Belmont, California
4. Bidwell,R.G.S. 1979. Plant Physiology. Mac. Millan. Publishing, New
York
5. Devlin, R.M. and F.H. Witham. 1983. Plant Physiology. The Towa State
University Press
6. Gardner,F.P.,R.B. Pearce and R.L. Mitchell.1985, Physiology of crop
plants
7. Hall.D.O. and K.K. Rao 1981. Photosynthesis, London
http://smtom.lecture.ub.ac.id/
Password: smtom-pphs-11

LECTURE 1: ENERGY

high energy photon

e- Compe-
tency

http:\\smtom.lecture.ub.ac.id Password: sm09tom1

An important part of understanding life is

understanding how energy is stored and moved from
molecule to molecule
 2. ENERGY
What is the importance of studying Energy in plant
physiology?
• As plant physiology is about HOW PLANTS
WORK, then sufficient understanding of
energy is important in the study of plant
physiology as
 2.1 Definition
Energy is the ability to do work
• Living organisms need energy for growth and
movement.
• Energy is the basic requirement of plant to
function
• Energy sustains the work of biosynthesis of
cellular and extracellular components, the
transport of ions and organic chemicals etc.
• Sunlight is the ultimate source of energy for life.
 Tides
 Primarily caused by the pull of the Moon on
Earth
 Not exactly 24
hours apart
 Causes two tidal
bulges

 Sun also causes tides

 Can work with or against the Moon’s force
 2.2 Energy Of Organisms
 Organisms are divided into two groups based on
the principal carbon source (Staniewr et al,
1977).
 Autotrophic: Organisms use inorganic carbon (plants)
 Heterotrophic: Organisms use organic carbon
(animals)
  Another division of organisms is based on
energy sources
 Phototrophic (or photosynthetic) Organisms use the
radiant (solar) energy
 Chemotrophic Organisms use the energy released
during chemical oxidations
 Combining these two basic criteria leads to the
recognition of four major nutritional
categories :
1. Photoautotrophs, utilizing light as an energy
source and C02 as the principal source of carbon
(e.g. higher plants, algae, cyanobacteria and the
purple and green sulphur bacteria).
2. Photoheterotrophs, dependent on light as a source
of energy and deriving much of their carbon from
organic compounds. This category is represented
by a specialized group of photosynthetic bacteria
known as non-sulphur purple bacteria.
3. Chemoautotrophs, deriving energy from
the oxidation of inorganic compounds
and using C02 as the principal carbon
source. This category comprises several
groups of specialized bacteria, including
the nitrifying bacteria and thiobacilli.
4. Chemoheterotrophs, utilizing organic
compounds as both energy and carbon
sources (e.g. animals, protozoa, fungi
and most bacteria).
 2.3 Law of Conservation of Energy
1. Energy can neither be created nor destroyed
2. Energy is always changing from one kind to another.
3. The total energy of an object never changes. The
amount of energy in the Universe is constant!!

 Potential energy + Kinetic energy = Total energy,

Total energy – Kinetic energy = Potential energy and
Total energy - Potential energy = Kinetic energy

Conservation of Energy is different from

Energy Conservation, the latter being about
using energy wisely
 2.4 State and Form of Energy
 State of Energy  Form of Energy
1. Kinetic Energy 1. Chemical Energy
2. Potential Energy 2. Mass Energy
3. Radiant Energy
4. Electrical Energy
5. Nuclear Energy
6. Thermal Energy
7. Sound Energy
8. Mechanical Energy
9. Magnetic Energy
 State of Energy
 Kinetic Energy
 Kinetic energy exists whenever an object which
has mass is in motion with some velocity.
Everything you see moving about has kinetic
energy.  The kinetic energy of an object in this
case is given by the relation:
KE = (1/2)mv2
m = mass of the object
V = velocity of the object
 The greater the mass or velocity of a moving
object, the more kinetic energy it has.
 Kinetic Energy
• The energy of motion.
• The faster the object moves – the
more kinetic energy.
• Kinetic energy depends on both
mass and velocity.
KE = ½(mass x velocity2)
• Kg m2/s2 =Newton*meter=
Joules

The greater the mass or

velocity of a moving object, the
more kinetic energy it has.
 Potential Energy
 Potential energy exists whenever an object
which has mass has a position within a force
field. The most everyday example of this is the
position of objects in the earth's gravitational
field. The potential energy of an object in this
case is given by the relation:
PE = mgh
PE = Energy (J = Joules)
m = mass (kg)
g = gravitational acceleration of the earth (9.8 m/s2)
h = height above earth's surface (m)
 1 W = 1 J/s
Example of Power from Niagara Falls
 Each kg of water gets kinetic energy of
KE = mgh
= 1 kg x 9,8 m/s2 x 58 m = 568 J
H = 58 m
Flow = 7.6.106 kg/s
 Each second, 7.6 million kg of water fall, or
P = 7.6 x 106 kg/s x 586 J/kg
= 4.3.109 J/s = 4.3109 W
 Form of Energy
1. Chemical Energy
 Chemical Energy is required to bond atoms together,
and when bonds are broken, energy is released.
 Burning
 a wooden match (0.3 grams) releases about
 1055 Joules >3,000 J/g, nearly 1 Cal/g
 gasoline yields about
 39 kJ per gram, or just over 9 Cal/g

 A 2000 Calorie per day diet means 20004184 J = 8,368,000

J per day8.37 MJ in (24 hr/day) (60 min/hr)(60 sec/min) =
86,400 sec  corresponds to 97 Watts of power
 2. Mass Energy
In the special theory of relativity Einstein
demonstrated that mass and energy
could be traded one for the other.

The relationship between the mass and the

energy is contained in what is probably the most
famous equation in science,
E = mc 2
 The energy equivalent of one gram of material
(any composition!!) is
(0.001 kg)(3.0108 m/s)2 = 9.01013 J = 90 TJ
 If one gram of material undergoes a chemical
reaction, losing about 9,000 J of energy, how much
mass does it lose?
9,000 J = mc2,
So
m = 9,000/c2 = 9103/91016 = 10-13 kg
2.5 Electron Energy
 Atoms are not indivisible
 Made up of protons, neutrons,
and electrons
 The nucleus contains protons
and neutrons

• Subatomic particles have charge (sometimes)

– Protons have positive charge, electrons have
negative charge, neutrons have no charge
 Terminology
• Atomic Number is how many protons an
A
X
atom has (Z)
• Atomic Mass Number is how many
protons and neutrons an atom has (A) Z
 Elements are defined by atomic number
 Different AMUs result in different isotopes
 12
C is “carbon 12”, 14C is “carbon 14” etc
 Mass of particles
 Electron = 0,00055 amu (atomic mass unit)
 Proton = 1,00728 amu
 Neutron = 1,00866 amu
 Positron = 0.00055 amu
 Deutron = 2,01355 amu
 Niel Bohr pada tahun 1913 menyajikan
gambaran novel atom yang terdiri dari
elektron yang mengorbit inti
 Elektron dapat mengorbit pada jarak yang
dekat dengan atau jauh dari inti, dan tempat
orbit ini tertentu

H Proton (+)

Elektron (-)
C
 Centrifugal force =
electrical attraction
between the proton
and the electron
Fe = kZe2/r2
r Fe k = 9.109 N.m2/C2
e = muatan elektron
= 1.60219.10-19C
Fc

v
Centripetal force
Z = jlh proton dalam inti
Fc = mv2/r r = jari-2 orbit
v = kecepatan elektron
 Bohr kemudian mengasumsikan bahwa ada orbit
tertentu dimana elektron stabil
 Elektron yang jauh dari inti dapat jatuh ke orbit yang
mendekati inti karena gaya centripetal diikuti dengan
kehilangan energi potensial (PE)
Mis. Energi total elektron pada orbit n =
En dan pada orbit p = Ep
Kehilangan energi dengan elektron jatuh
dari orbit n ke p ad.
En – Ep
 Energi elektron pada orbit tertentu (n)
dapat diestimasi dengan persamaan
berikut
En = -(kZe2/2)(kZe242m/n2h2)
En = -22k2e4Z2m/n2h2 ……..(9)
 = 22/7
k = 9.109 N.m2/C2
e = 1.60219.10-19 C
m = 9.1095.10-31 kg
h = 6.6262.10-34 Js
 Untuk atom yang mempunyai nomor
atom Z (proton + neutron) = 1,
persamaan diatas dapat disederhanakan
menjadi
En = -13,6/n2 eV ……………….(10)
1 J = 6,25.1018 eV
Illustration
Singly ionized helium atom which has lost
one of its two electrons. Draw the
energy-level diagram for this ion
Reasoning
The singly ionized helium atom will be
much like hydrogen atom except that the
change on nucleus is +2e, and so Z = 2.
From equation, it is found
En = -54,4/n2 eV
Sehingga
E1 = -54.4 eV
E2 = -13,6 eV
E3 = -6,04 eV
E4 = -3,42 eV
 2.6 Free Energy
 The Gibbs free energy is one of the most important
thermodynamic functions for the characterization of a
system
 Gibbs free energy is defined in 1876 by Josiah Willard
Gibbs to predict whether a process will occur
spontaneously at constant temperature and pressure.
Gibbs free energy, also indicating how much work is
attainable for any given process, is defined as
 G = H – TS
where
G is the Gibbs free energy, measured in joules
H is the enthalpy, measured in joules
T is the temperature, measured in kelvins
S is the entropy, measured in joules per kelvin
  Enthalpy (H) is a measure of the energy
associated with a system, and defined as:
H = U + pV
where
H is the enthalpy of the system (in joules),
U is the internal energy of the system (in
joules),
p is the pressure at the boundary of the
system and its environment, (in pascals),
and
V is the volume of the system, (in m3).
 Note that the U term is equivalent to the energy
required to create the system, and that the pV
term is equivalent to the energy which would be
required to "make room" for the system if the
pressure of the environment remained constant.
 Enthalpy is sometimes described as the "heat
content" of a system under a given pressure.
Such a visualization assumes no energy
exchange with the environment other than
heat or expansion work. Given such
restrictions, it can be shown that:
 The enthalpy is the total amount of energy which
can the system can emit through heat,
 Adding or removing energy through heat is the only
way to change the enthalpy, and
 The amount of change in enthalpy is equal to the
amount of energy added through heat.
 tightly bound molecules have higher heat energy.
 Entropy (S) is a measure of the disorder in
a system.
 Molecules distributed randomly have high
entropy (large S) while ordered molecules have
low entropy (small S).
 The quantity of G cannot be measured
experimentally, but the change of free
energy (G) or the maximum amount of Ice melting in a warm
room is a common
energy made available can be evaluated. example of "entropy
G = H − TS increasing", described
in 1862 by Rudolf
 A chemical reaction will have a H < 0 if the Clausius as an increase
heat energy of the reactants is greater than the in the disaggregation
products. of the molecules of the
 A reaction will have S < 0 if the reaction body of
results in increased order and S > 0 if the
reaction results in increased entropy.
 When the concentrations of reactants and products are
variable for the following reaction
AB
we can determine G as
[B ]
G  G 0 '  R  T. ln
[ A]

where R is the universal gas constant, T is temperature, and

[B] & [A] are the initial concentrations of the products and
reactants.
 We can plot G as a function of [B]/[A] to see how the free
energy of the reaction changes as reactants are converted
to product ([B]/[A] increases).
 Process Chemical Reaction Go'
(kcal/mol)
photosynthesis 6CO2 + 6H2O --> glucose + 6O2 +686
hydrolysis of Sucrose + H2O --> glucose + -7.0
sucrose fructose

conversion of ATP + H2O --> ADP + phophate -7.3

ATP to ADP

esterification glucose + phosphate --> glucose +3.3

6-phosphate + H2O
 As an example, the chemical reaction of photosynthesis has
a standard free energy
Go' = +686 kcal/mol
The reverse reaction has
Go' = -686 kcal/mol
 Penentuan G
 Jika suatu reaksi berlangsung, mis. zat A
berubah menjadi zat B seperti berikut

 GB > GA = reaksi bersifat eksergonik

apabila (energi dibebaskan )
 GB  GA = reaksi bersifat endergonik
(energi digunakan )
aA + bB cC + dD + free energy

cC + dD + free energy aA + bB

[B] [C]c  [ D]d

Keq  Keq 
[A] [A ]a  [B]b

G  G 0
 RT ln
B
A

G  G 0 C  D
 RT ln a
c d

A  Bb
Standard
 Kimia fisik :
 konsentrasi reaktan & produk = 1 M, dan G & G0
dinyatakan pada pH = 0
 Biokimia :
 konsentrasi reaktan & produk = 1 M kecuali [H+] =
10-7 M, G’ & G0’ dinyatakan pada pH = 7

 Jadi perubahan energi bebas standar berbeda

antara biokimia dan kimia fisik untuk reaksi
yang melibatkan ion hidrogen
 Untuk reaksi yang melibatkan H + sebagai produk
 Pada keadaan standar, [A] = [B] = [C] = 1 M dan [H+] = 10-
7
M, sehingga
G0’ = G0 + RT ln[H+]x = G0 + x RT ln10-7
 Jika x = 1, maka pada 2980 K
G0’ = G0 –39,95 kJ atau G0 = G0’ + 39,95 kJ
 Jadi G0  G0’ sebesar 39,95 kJ/mol H+ yang dibebaskan untuk
rekasi yang melibatkan H+. Ini berarti reaksi akan lebih spontan
pada pH = 7

 Sebaliknya, reaksi yang melibatkan H+ sebagai reaktan

G0 = G0’ - 39,95 kJ

 Sehingga reaksi akan lebih spontan pada pH = 0
Ilustrasi 1.
Jika glucose 1-phosphate (G1-P) dikonversi ke G 6-P
oleh enzim phosphoglucomutase pada 250C dengan [G
1-P) turun dari 0,02 M menjadi 0,001 M bersamaan
dengan peningkatan [G 6-P) menjadi 0,019, hitunglah
G0

Reasoning
Konsentrasi substrat, [G 1-P) = 0,02 dan
produk, [G 6-P) = 0,019, sehingga
Keq = 0,019/0,001 = 19
G0 = -RT ln Keq = -1363 logKeq
= -1363 log 19 = -1745 cal
Ilustrasi 2.
Apabila G0’ dari hidrolisis ATP ke ADP+Pi = -7,3
kcal.mol-1, hitunglah Keg reaksi tersebut
Reasoning
G0’ = -RT ln Keq’
-7,3 kcal.mol-1 = -(1,98.103 kcal.0K-1.mol-1)
(29800K)(2,303 log Keq’)
Log Keq’= 5,35 ; Keq = 2,2.105

Ilustrasi 3.
NAD+ dan NADH ad. btk oksidasi dan reduksi nicotinamide
adenine dinucleotide. Harga G0 untuk oksidasi NADH = -21,83
KJ.mol-1 pada 2980K. Hitunglah G0, Keq’ dari reaksi tsb. Hitung
juga G dan G’ jika [NADH] = 1,5.10-2, [H+] = 3.10-5, [NAD+] =
4,6.10-3 dan pH2 = 0,01 atm. Do it by yourself if you like
 Tugas
1. How do plants work to live
2. What is the function of cell components
3. What is energy ?
4. Where do plants for the first time derive
energy from ?
5. What does it mean by potential energy ?
6. How much is the mass energy of 0,5 kg body ?
7. How much is the free energy of ATP
hydrolysis at pH = 7, 250C and steady state
when the concentration of ATP, ADP dan Pi is
10-5 M, 10-3 M dan 10-7 M (G0’ of ATP = 7700
cal.mol-1) respectively ?
TERIMAKASIH

THANK YOU
Спасибо
謝謝
ً‫شكرا‬
THANK YOU
Спасибо

謝謝
ً‫شكرا‬