Yash

Study on Phytochemical and ex-vivo Anti-Cancer potential of
bark of Neolamarckia Cadamba

Practice School Report
(BP706PS)
A
Project Submitted to
Dr.
A. P. J. Abdul Kalam Technical University,
Lucknow, Uttar Pradesh
Submitted By Submitted To
Payal Teotia Dr. Prasoon Kumar Saxena
Priyanshu Sharma
Yash Gupta
Vimal Kishor
SUNDER DEEP PHARMACY COLLEGE

NH-9, Delhi-Hapur Road, Ghaziabad, Uttar Pradesh India
CANCER
CANCER is defined as an
uncontrolled proliferation of cells,
which invade & damage the
surrounding normal cell.
Normal cells show contact inhibition
property, by virtue of which contact
with other cells inhibit their
uncontrolled growth.
Cancer cells loss the property of
contact inhibition and continue to
divide giving rise to masses of cells
called TUMOURS.
Cancer is not one disease, but a group
of more than 100 different forms in
each area.
Cancer is the 2nd leading cause of
death in the U.S., surpassed only by
heart disease.
TYPES OF TUMOUR
1. Benign Tumour
 They remain confined to their
original location and do not
spread to other part.
 They are Non- cancerous
tumours.
 They cause little damage to
the body.
2. MALIGNANT TUMOUR
 Malignant tumours spread to other areas in body.
 They are masses of proliferating cell which grow very rapidly,
invade & damage the surrounding normal tissue, starving the
normal cell by competing for vital nutrient.
 They are cancerous tumour.
CAUSES OF CANCER
 Cancer may be induced by physical, chemical & biological
agent Carcinogenes.
 Chemical Carcinogenes like tobacco smoke is the major cause
of lungs cancer.
CANCER DETECTION & DIAGNOSIS
Cancer Detection is based on biopsy and histopathological study
of the tissue, blood and bone marrow test for increase cell
count in case of Leukemia’s.
 In biopsy, a piece of the suspective tissue cut into thin sections
is stained and examined under the microscope.
 Techniques like radio graph (X-rays), CT (computed to
mography) & MRI (Magnetic Resonance Imaging) are used to
detect cancer of the internal organs.
 CT uses X-rays to generate a
three dimensional image of the
internal organs.
 MRI uses strong magnetic field
and non- ionising radiation to
detect pathological &
physiological changes in the
tissue.
 Antibodies against cancer
specific antigens are also used
for detection of certain cancer.
 Techniques of molecular
biology is also used to detect
genes with inherited
successiptibility to certain
cancer.
TREATMENT
 Surgery
 Chemotherapy
 Radiation
 Targeted Cancer Treatments
 Small Molecular Inhibitors
 Antibodies
 Cell Based Immunotherapy
 Gene Therapy
TREATMENT OF CANCER
There are following methods for treatment of cancer:
1. Surgery: The cancerous tissue is removed surgically to
reduce the load of cell.
2. Radiation Therapy: The tumour cell are irradiated leathally
taking care of the surrounding tissue.
3. Chemotherapy: Chemotherapeutic drugs are to kill cancerous
cells, however majority of drug have side effect like hair
loss, anaemia etc.
4. Immunotherapy: It involves the use of biological response
modifier like interferons, which activates Immune system &
help in destroying the tumour.
DRUG USED TO TREAT CANCER
HERBAL DRUGS USED TO TREAT
CANCER
HERBAL PLANTS USED TO TREAT
CANCER
 Leaves of Catharanthus Roseus

 Bark of Peruvian Cinchona Tree.
 Bark of Neolamarckia Cadamba

NEOLAMARCKIA CADAMBA
 Neolamarkia Cadamba
 Family : Rubiaceae
 Kingdom : Plantae
 Synonym : Neonauclea megaphylla ,
Nauclea cadamba
 Common Name : Bur-flower Tree, Kaddam,
Laran
 Chemical Constituents : Cadambagenic acid,
cadamine, quinovic acid, β-sitosterol,
cadambine, terpinolene, Campene.
 Biological Source : South and Southeast
Asia lower hills of Assam, orrisa, Bihar.
COLLECTION
 Neolamarckia Cadamba barks was collected from Sunder
deep college campus.
 Then the barks were washed under tap water to remove
excess dust and particles.
EXTRACTION
 Now, partially solubilized solution is filtered with filter paper
which was obtained from orbital shaker.
 Then the filtered extract is obtained in a beaker separately
and residue is discharged.
 After that the extract is shifted into china dish for decoction.
 Then the china dish is kept on heating mantle at 60 oC for

drying to extract out the drug.
 After 5-10 minutes dried extract is obtained
MITOSIS
CORRELATION BETWEEN CANCER AND
MITOSIS
Mitosis is the process of cells growing and dividing, hence
replicating themselves. Cancer is simply uncontrolled cell
division. In the cell, mitosis is always tightly regulated. If the
cell has errors in it (faulty DNA, for example), the regulator
proteins will not allow it to divide.
ACTIVITY
 First of all, three Onion are taken and kept for their root growth
in which the roots are dipped in water for 5 days and observed.
 After 5 days all the three onion have a good growth of roots and
Shots .
 In next Step these three onions are dipped in different Beakers
A, B, C containing different Solution for 1 day {Beaker A
contains Sample Drug i.e, extract of Neolamarckia Cadamba
having 5mg/ml concentration Beaker B contains Standard Drug
i.e, Colchicine having 1mg/ml concentration Beaker C Contains
Distilled water}.
 After one day we observed the Change in growth of Roots of
onion.
OBSERVATION AFTER 2 DAYS
FIG.1 FIG.2
REFERENCE
Bhanot, J. P., Verma, A. N., Batra, G. R. (1991) Effect of seed treatment with different
insecticides on germination, damage by termite (Microtermes obesi) and yield of wheat
(Triticum aestivum L.). Indian J. Agricul. Sci. 61, 688–691.
MICROSCOPY
A. STANDARD B. SAMPLE C. DISTILLED
WATER
CONCLUSION
Anticancer activity can refer to various outcomes observed when testing substances
for their ability to inhibit or destroy cancer cells. Results can vary widely depending
on the substance tested, the type of cancer, and the specific experimental conditions.
Some substances show promising results in inhibiting cancer cell growth, inducing
apoptosis (cell death), or reducing tumor size in preclinical studies or clinical trials.
However, it's crucial to note that not all promising results in laboratory studies
translate into effective treatments for humans due to complexities in the human body
and the unique nature of each cancer type. Success in anticancer activity in
experimental settings doesn't always guarantee the same outcome in clinical
applications on the basis of our experiment we observed that taken sample drug was
effective against Cancer as it stop the growth of Cancerous cells.

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Study on Phytochemical and ex-vivo Anti-Cancer potential of

bark of Neolamarckia Cadamba

SUNDER DEEP PHARMACY COLLEGE

 Targeted Cancer Treatments

 Small Molecular Inhibitors

 Cell Based Immunotherapy

 Leaves of Catharanthus Roseus

 Bark of Neolamarckia Cadamba

 Then the china dish is kept on heating mantle at 60 oC for

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