Chapter - 2 Short
Chapter - 2 Short
Chapter - 2 Short
Chapter # 2
Biological Molecules
Short questions and answers
Q. Define biochemistry. Give its importance.
Biochemistry
Def: - “It is a branch of biology which deals with the study biochemical compounds and
chemical processes in the body of living organisms”.
Importance of biochemistry:-
It helps to get information about structure and formation of biochemical compounds like
proteins, carbohydrates etc.
It explains about abnormal reactions causing disease in our body so these could be treated
well by medicines.
It also explains about the messages transport mechanism in neurons.
It gives information about the chemical basis of cellular differentiation from fertilized egg to
different body organ tissues.
It explains about the growth of cells
It explains about the mechanism of memory
Introduction:
The body of all living organisms is composed of cells and a cell contains a living material called
Protoplasm. It contains enormous biochemical compounds and is responsible for all vital functions
of life. Protoplasm contain about 70 to 90% water when water evaporates remaining mass is the
dry weight of cell. The biochemical present in it mainly composed 98% of these six elements C, N 2,
P, H2, O2 and S.
Water is present in all living cells. Its composition varies from 70 to 90% from cell to cell.
Chemical nature of Water:-
A water molecule is chemically composed of two Hydrogen atoms and one Oxygen atom. Water is a
polar molecule i.e. it ahs slightly negative end, oxygen and slightly positive end, Hydrogen atoms.
This separation of electrical charge is called dipole. This property helps H 2O molecule to form
hydrogen bonds.
Water is called a universal solvent due to its polar nature and dissolve substances many polar and
can also react with non polar substances.
Q. How biochemical reactions carried out at constant rate and cellular contents remain stable in
water?
The specific heat of water is high therefore it is slow to release and absorb heat. This thermal
stability plays an important role in biochemical reactions to carried out at fairly constant rate.
Due to strong hydrogen bonding water requires high amount of heat to change in to vapor form.
Similarly needs greater amount of energy to freeze. Therefore cellular contents remain stable.
Water acts as both acid and a base, so it is an amphoteric molecule. Due to this property it acts as
the best suitable medium for chemical processes particularly metabolism by maintaining its pH as
buffer in the living cell.
The molecules contain carbon and hydrogen elements necessarily with other elements are
called organic molecules. These are formed inside the cell in the following state.
“The process in which macromolecules or polymers are broken down into smaller monomers or
sub-units by the addition of water is called hydrolysis”.
During this process a water molecule breaks in to +H and –OH ions by the help of enzyme,
whereas –OH group attaches to one monomer and a –H attaches to the other. When a bond is
broken, energy is released or made available. E.g. In animals large molecules are digested by the
activity of enzyme called hydrolytic enzyme.
Carbon is a unique element it forms number of compounds which vary widely in their properties
and adaptation. Study of these carbon containing compounds are called organic chemistry.
Carbon has atomic number 6 and contains 4 electrons in its outmost shell so it can combine with
four atoms by covalent bond.
Carbon is capable of forming bonds with other elements like Oxygen, Nitrogen, Sulphur,
Phosphorus, and Halogens (Cl, Br, I) and with electropositive element Hydrogen.
“A chemical bond formed by the mutual sharing of electrons in between at least two same or
different elements is called covalent bond”.
Carbon forms three types of covalent bonds.
1. Single Covalent bond: This bond is form by the mutual sharing of one
pair between the atoms.
CH3------CH3
Ethane
2. Double Covalent bond: This bond is form by the mutual sharing of two
pair between the atoms.
CH3======CH3
Ethene
3. Triple Covalent bond: This bond is form by the mutual sharing of three
pair between the atoms.
CH3_____CH3
Ethyne
Functional Group: These are particular group of atoms that behave as a unit and give organic
molecules their physical, chemical properties and solubility in aqueous solution.
Some functional groups are as follows
The most common linkages formed between functional groups are as follows
1) Ester bond: - Bond formed between carboxylic acid and alcohol.
2) Amide bond: - A bond formed between carboxylic acid and amine.
Proteins are the most important building block of all the living organisms’ body. These are large
molecules with structural and metabolic functions. Keratin, which makes up hair and nails, and
collagen fibers, which support many organs, are examples of structural proteins.
The name protein was suggested by Berzelius in 1838 and the Dutch Chemist G.J. Murlder in
1883 recognized the importance of protein as vital compound. Proteins are complex organic
compounds having H, C, O, and N as elements but sometimes they contain P and S also. Due to the
presence of N they are called nitrogenous compounds. Egg, meat, fish, pulses are the major source
of proteins.
Def: - “An organic compound containing one or more amino groups (-NH 2 ) and one or more
carboxyl groups (-COOH) is known as amino acid”.
The amino acids occur freely in the cytoplasmic matrix.
The amino acids are derived from the organic acids in which the hydrogen in alpha position is
replaced by the amino group. For example the amino acid glycine is formed by the acetic acid and
alanine derived from propionic acid.
Amino acids are linked together by specialized bond or linkage called peptide linkage.
The cytoplasm contains about 20 amino acids which are classified in to two groups; hydrophilic
amino acids (11) and hydrophobic amino acids (9).
When two molecules of amino acids are combined then the basic group (-NH 2) of one amino acid
molecule combines with the carboxylic (-COOH) group of other amino acid and the loss of a water
molecule takes place. This condensation of two amino acid molecules by –NH-CO linkage or bond is
known as peptide linkage or peptide bond resulting the formation of polypeptide chain formation of
protein.
Structure of proteins:
There are four basic level structures of proteins:
1) Primary structure: A polypeptide chain having a linear sequence of amino acid having disulphide
(S-S) bond is called primary structure. This structure was discovered by Frederick Sanger and his
co-workers at Cambridge University in 1955.
2) Secondary structure: When a polypeptide chain of amino acids become spirally coiled this
structure is called Secondary structure of protein. This is a tubular and rigid structure called
helix.
3) Tertiary structure: The term Tertiary structure refers to the irregular loops or bends making
three dimensional polypeptide structures.
4) Quaternary Structures: It is the association of more than one polypeptide chain into large sized
molecules called quaternary structure.
1. Monosaccharides (Monomers),
2. Oligosaccharides (Oligomers), and
3. Polysaccharides (Polymers).
These are called simple sugars with the empirical formula C n(H2O)n. They are classified and named
according to the number of carbon atoms in their molecules as follows. These are found in various
fruits and vegetables e.g. Glucose is a primary source of energy for the cell found in ripe fruits,
honey, sugar cane etc. Fructose is also present in fruits and called fruit sugar. Galactose is found in
combined state in lactose (milk).
Bloor in 1943 proposed the term lipid according to him, “These are organic compounds which are
insoluble in the water but soluble in organic solvent”.
The lipids are non polar and hydrophobic. They are the important constituents of the cellular
membranes, hormones, and vitamins of the cells. They also form the source of energy for the cells.
Generally these are called fats and oils and found in animals and plants.
These compounds posses double amount of energy then Carbohydrates by having C-H bonds. About
16 Kg fats or 144000 k cal energy present in a person of average size.
Acylglycerol is also called a Triacyglyceride because of three fattyacids linked with one glycerol
molecule.
There are two types of Acylglycerol.
Waxes belong to the group of biological molecules called lipid. In waxes, a long chain of fatty acid
combines with a long chain of alcohol by ester bonding. Being hydrophobic, they are also water
proof and resistant to degradation. In many plants waxes form a protective cuticle (covering) that
retards the loss of water for all exposed parts. In animals, waxes are involved in skin and fur
maintenance. In humans wax is produced by glands in the outer ear canal here its function is to trap
dust and dirt particles.
Initially 22 years old a Swedish Friedrich Miescher isolate a nuclear substance and named nuclein,
later on it was named nucleic acid. These nucleic acids are present in all living organisms in the form
of DNA and RNA which often remain combined with nuclear proteins. In a typical eukaryotic nucleus
thousand times more DNA than a bacterium and a hundred thousands times more than a virus is
found. Nucleic acids are composed of large numbers of nucleotides like proteins and the
arrangements of nucleotides in a chain manner is called polynucleotide or polymer of nucleotide.
Definition: “Portion of DNA or RNA molecule composed of one Deoxyribose phosphate unit (in DNA)
or one ribose phosphate unit (in RNA) in addition with Purine or a Pyrimidine is called a
Nucleotide”.
Pentose sugar
“Sugar molecules contain five carbon atoms called pentose”. These are of two types Ribose
(C5H10O5) or Deoxyribose (C5H10O4) which serves as a basic skeleton in DNA or RNA.
Nitrogen bases
“The nitrogen containing organic compounds of DNA or RNA are called nitrogenous bases”.
There are two basic types of nitrogen bases Purines and Pyrimidine.
Purine includes Adenine and Guanine.
Pyrimidine includes cytosine, thymine and uracil.
Mononucleotide
“Mononucleotide is the molecules used to caries energy and exists singly in the cell”.
Some of these have extra phosphate group e.g. ATP (adenosine triphosphate).
Dinucleotide
“When two nucleotide are covalently bounded together, these compounds are called dinucleotide”.
The best example of dinucleotide is Nicotinamide adenine dinucleotide (NAD).
Polynucleotide
Polynucleotides include nucleic acids. These are considered as central dogma of life. These are
biological macromolecules serves as repositors of genetic information and precursor to determine
the path of growth and development in living organisms and control the synthesis of different
biomolecules like proteins etc. Both RNA and DNA are polynucleotide.
1. ATP 2. NAD
ATP
The ATP consists of a Purine base adenine, a pentose sugar ribose and three molecules of the
phosphoric acid. in ATP the last phosphate group is linked with ADP by a special bond “ because
when the last phosphate group of the ATP is released the large amount of energy is released as
shown by the following reaction.
NAD
NAD is a Co-enzyme [Coenzymes are the molecules (non-protein) which bind to enzyme (Protein)
and serves as a carrier for chemical groups or electrons] that carries electron and work with
The DNA has a key role in all the biosynthetic and hereditary functions of the living organisms. The
most important function of the DNA is that it acts as a carrier of genetic information from
generation to generation. It is the most stable compound of the biological world which can be
considered as immortal.
This can be proved by transformation process in bacteria and infection of bacteria by bacteriophage
where it directs the synthesis of more phages. Hershey and Chase thus experimentally confirmed
that DNA must be the genetic material.
In view of molecular geneticists, a gene is a sequence of DNA nucleotide bases that codes for a
product. DNA has specific sequence of nitrogenous bases. These four bases shows enormous
combinations in which each of these combinations refer to specific information as we see in protein
synthesis where amino acids are represented by different pairs of codons. Thus these combinations
representing specific information and are called genes. e.g. a piece of DNA with 10 nucleotide and
exists in over a million different possible sequences of four bases these all are considered as genes
which represents specific characters.
RNA is considered as a carrier of information because it receives and transmits the information
from the DNA and act accordingly in different biochemical processes.
DNA synthesis RNA which carries information from DNA to the cytoplasm. There are three types of
RNA’s present in cytoplasm that perform different functions.
Messenger RNA (mRNA): This carries information from DNA to cytoplasmic matrix.
Transfer RNA (tRNA): These are present in the cytoplasm and act according to the
instructions coming from DNA through mRNA.
Ribosomal RNA (rRNA): These are present in the ribosomes and providing a platform for the
attachment of both tRNA and mRNA.
Activities of RNA’s can be seen in the process of protein synthesis which is a two step process.
Translation
In this step information receive in ribosome where rRNA and tRNA work according to the
instruction and synthesize the protein.
These are the biomolecules which are formed by the combination of two different molecules.
Following are the types of conjugated molecules.
i) Glycolipids
Def; “Lipids containing fatty acids carbohydrates and nitrogenous compounds are called
Glycolipids”. These are important constituent of brain and also called cerebrosides.
ii) Nucleoproteins
Def: - “These are found in the nucleus conjugated with nucleic acid”. These are weakly acidic and
soluble in water.
iv) Lipoproteins
Def:- “ Lipids and proteins combined together and form molecules are called Lipoproteins”.
These help in the transportation of lipid in the blood plasma.
They also present in cellular organelles.
These occur in the hyaline sheath of nerves, photoreceptive structures, chloroplast and the
membranes of bacteria.
Descriptive questions
Q. Describe the Biological properties of water.
Best Solvent: Water is called a universal solvent due to its polar nature and dissolve
substances many polar and can also react with non polar substances.
High heat capacity: The specific heat of water is high therefore it is slow to release and
absorb heat. This thermal stability plays an important role in biochemical reactions to carried
out at fairly constant rate.
High Heat of vaporization: Due to its hydrogen bonding water requires high amount of heat to
change in to vapour form. Similarly needs greater amount of energy to freeze. Therefore cellular
contents remain stable.
An amphoteric molecule: Water acts as both acid and a base, so it is an amphoteric molecule.
Due to this property it acts as the best suitable medium for chemical processes particularly
metabolism by maintaining its pH as buffer in the living cell.
Proteins
Proteins are the most important building block of all the living organisms’ body. These are large
molecules with structural and metabolic functions. Keratin, which makes up hair and nails, and
collagen fibers, which support many organs, are examples of structural proteins.
The name protein was suggested by Berzelius in 1838 and the Dutch Chemist G.J. Murlder in 1883
recognized the importance of protein as vital compound. Proteins are complex organic compounds
having H, C, O, and N as elements but sometimes they contain P and S also. Due to the presence of
N they are called nitrogenous compounds. Egg, meat, fish, pulses are the major source of proteins.
Functions of Proteins:
Def:- “ An organic compound containing one or more amino groups (-NH 2 ) and one or more
carboxyl groups (-COOH) is known as amino acid”.
The amino acids occur freely in the cytoplasmic matrix.
The amino acids are derived from the organic acids in which the hydrogen in alpha position is
replaced by the amino group. For example the amino acid glycine is formed by the acetic acid
and alanine derived from propionic acid.
Amino acids are linked together by specialized bond or linkage called peptide linkage. Where R is
the radical group, it shows the variety of structures.
The cytoplasm contains about 20 amino acids which are classified in to two groups; hydrophilic
amino acids (11) and hydrophobic amino acids (9).
When two molecules of amino acids are combined then the basic group (-NH 2) of one amino acid
molecule combines with the carboxylic (-COOH) group of other amino acid and the loss of a water
Structure of proteins:
Carbohydrates
Carbohydrates are the compounds of the carbon, hydrogen and oxygen. They generally represented
as (CH2O)n and are called hydrate of carbon. Only green parts of plants and certain microbes have
the power of synthesizing the carbohydrates form the water and CO 2 in the presence of sunlight and
chlorophyll by the process of photosynthesis.
1. Monosaccharides (Monomers),
2. Oligosaccharides (Oligomers), and
3. Polysaccharides (Polymers).
1. Monosaccharides (Monomers)
These are called simple sugars with the empirical formula C n(H2O)n. They are classified and named
according to the number of carbon atoms in their molecules as follows.
Oligosaccharides
Polysaccharides
The polysaccharides are composed of ten to many thousands monosaccharide as the monomers
in their molecules. These are of two kinds.
Homopolysaccharides contains similar kinds of monosaccharides in their molecules. e.g. starch,
glycogen an cellulose.
Heteropolysaccharides contains different kinds of monosaccharides in their molecules.
Functions of Carbohydrates:
Carbohydrates are the major source of energy.
It is also act as storage food in the form of starch in plants and glycogen in animals.
Cellulose forms the cell wall of the plant cells and provides mechanical support to the cell.
The hyaluronic acid (Muccopolysacchrides) forms the cementing material of the connective
tissues. it occurs in the skin, connective tissues and synovial fluid of the joints.
The chondroitin sulphate occurs in the cells of the cartilage, skin, cornea, umbilical cord and it
serves as a matrix for the bone formation.
Chitin which is similar to cellulose and serve as a structural polysaccharide for fungi and certain
other lower plants and as the exoskeleton of many insects.
Lipids
Bloor in 1943 proposed the term lipid,“ these are organic compounds which are insoluble in the
water but soluble in organic solvent”.
The lipids are non polar and hydrophobic.
They are the important constituents of the cellular membranes, hormones, and vitamins of the
cells.
They also form the source of energy for the cells.
The important groups of lipids are as follows.
Generally these are called fats and oils and found in animals and plants.
These compounds posses double amount of energy then Carbohydrates by having C-H bonds.
About 16 Kg fats or 144000 k cal energy present in a person of average size.
Acylglycerol is also called a Triacyglyceride because of three fattyacids linked with one glycerol
molecule.
There are two types of Acylglycerol.
1. Saturated Acylglycerol
2. Unsaturated Acylglycerol
They contain one or more than one double bond between carbon atom.
They are liquid at ordinary temperature.
They are found in plants and also called oil. e.g. linolin present in cotton seed.
Waxes
In waxes, a long chain of fatty acid combines with a long chain of alcohol by ester bonding.
Being hydrophobic, they are also water proof and resistant to degradation.
In many plants waxes form a protective cuticle (covering) that retards the loss of water for all
exposed parts.
In animals, waxes are involved in skin and fur maintenance.
In humans wax is produced by glands in the outer ear canal here its function is to trap dust and
dirt particles.
Phospholipids
These are similar to Tryacylglycerol or an oil except in place of fatty acid is replaced by
phosphate group.
A phosphate group is a polar group that can ionize and therefore is hydrophilic.
When Phospholipids molecules are placed in water, they form a double layer in which the polar
heads face outward and the non polar tails face each other. This property of phospholipids
means that they can form an interface or separation between two solutions, such as the interior
and exterior of a cell. Therefore helps in cell permeability and transport processes.
Terpenoids
Steroids
These consists of four fused carbon ring and varies from other steroids primarily by the type of
functional groups attached to the rings.
Carotenoids
The carotenoids are the compound lipids and they form the pigments of the animals and plant
cells.
There are about 70 carotenoids occurring in both type of cells.
Chemically carotenoids are propyrines which are linked with metals and proteins and forming
the important pigments of animals and plant cells such as the chlorophyll and haemoglobin.
Carotenoids are red, orange, yellow, cream and brown in colours in plants.
Some important carotenoids are carotene, xanthophylls etc.
Nucleic Acid
Initially 22 years old a Swedish Friedrich Miescher isolate a nuclear substance and named nuclein,
later on it was named nucleic acid.
These nucleic acids are present in all living organisms in the form of DNA and RNA which often
remain combined with nuclear proteins.
In a typical eukaryotic nucleus thousand times more DNA than a bacterium and a hundred
thousands times more than a virus is found.
Nucleic acids are composed of large numbers of nucleotides like proteins and the arrangements of
nucleotides in a chain manner is called polynucleotide or polymer of nucleotide.
Composition of Nucleotide
Definition: “Portion of DNA or RNA molecule composed of one Deoxyribose phosphate unit (in DNA) or
one ribose phosphate unit (in RNA) in addition with Purine or a Pyrimidine is called a Nucleotide”.
Pentose sugar
“Sugar molecules contain five carbon atoms called pentose”. These are of two types Ribose
(C5H10O5) or Deoxyribose (C5H10O4) which serves as a basic skeleton in DNA or RNA.
Nitrogen bases
“The nitrogen containing organic compounds of DNA or RNA are called nitrogenous bases”.
There are two basic types of nitrogen bases Purines and Pyrimidine.
Purine includes Adenine and Guanine.
Pyrimidine includes cytosine, thymine and uracil.
Mononucleotide
“Mononucleotide are the molecules used to caries energy and exists singly in the cell”.
Some of these have extra phosphate group e.g. ATP (adenosine triphosphate).
Dinucleotide
“When two nucleotide are covalently bounded together, these compounds are called dinucleotide”.
The best example of dinucleotide is Nicotinamide adenine dinucleotide (NAD).
Polynucleotide
Polynucleotides include nucleic acids. These are considered as central dogma of life. These are
biological macromolecules serves as repository of genetic information and precursor to determine
the path of growth and development in living organisms and control the synthesis of different
biomolecules like proteins etc. Both RNA and DNA are the examples of polynucleotide.
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