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    Determination of Yield of Silver Nanoparticles Using Atomic Absorption Spectroscopy (AAS)

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    Afrah M
    The document discusses two studies: 1) Using atomic absorption spectroscopy (AAS) to analyze the conversion of silver ions to silver nanoparticles (AgNPs) during synthesis, finding a 97.14% yield after 20 minutes of centrifugation. 2) Testing the antibacterial activity of synthesized AgNPs against E. coli and S. aureus, finding the highest activity against E. coli and the lowest against S. aureus, with increased effectiveness compared to silver nitrate due to the AgNPs' larger surface area and ability to damage cell membranes and DNA.

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    Determination of Yield of Silver Nanoparticles Using Atomic Absorption Spectroscopy (AAS)

    Uploaded by

    Afrah M
    52 views2 pages
    The document discusses two studies: 1) Using atomic absorption spectroscopy (AAS) to analyze the conversion of silver ions to silver nanoparticles (AgNPs) during synthesis, finding a 97.14% yield after 20 minutes of centrifugation. 2) Testing the antibacterial activity of synthesized AgNPs against E. coli and S. aureus, finding the highest activity against E. coli and the lowest against S. aureus, with increased effectiveness compared to silver nitrate due to the AgNPs' larger surface area and ability to damage cell membranes and DNA.

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    The document discusses two studies: 1) Using atomic absorption spectroscopy (AAS) to analyze the conversion of silver ions to silver nanoparticles (AgNPs) during synthesis, finding a 97.14% yield after 20 minutes of centrifugation. 2) Testing the antibacterial activity of synthesized AgNPs against E. coli and S. aureus, finding the highest activity against E. coli and the lowest against S. aureus, with increased effectiveness compared to silver nitrate due to the AgNPs' larger surface area and ability to damage cell membranes and DNA.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    52 views2 pages

    Determination of Yield of Silver Nanoparticles Using Atomic Absorption Spectroscopy (AAS)

    Uploaded by

    Afrah M
    The document discusses two studies: 1) Using atomic absorption spectroscopy (AAS) to analyze the conversion of silver ions to silver nanoparticles (AgNPs) during synthesis, finding a 97.14% yield after 20 minutes of centrifugation. 2) Testing the antibacterial activity of synthesized AgNPs against E. coli and S. aureus, finding the highest activity against E. coli and the lowest against S. aureus, with increased effectiveness compared to silver nitrate due to the AgNPs' larger surface area and ability to damage cell membranes and DNA.

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    IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)

    Determination of yield of silver nanoparticles using atomic absorption spectroscopy


    (AAS)

    AAS analysis was carried out to analyze unreacted Ag + ion concentration, which shows the
    conversion of Ag+ ions into AgNPs (Choudhary et al., 2016).
    The conversion of Ag+ to AgNPs and percentage yield of AgNPs increases with increasing
    centrifugation time. From the point of view of time and energy, 20 min of centrifugation time is
    selected. In the present study, it is comparatively high yield of AgNPs (97.14%) and took about 20
    min of centrifugation which is quite more than yield of AgNPs (87%) reported in the literature
    (Mehmood et al., 2016).

    Antibacterial activity of silver nanoparticles

    Experimental results are expressed as mean ± standard deviation (SD). Biosynthesized AgNPs
    shows the highest antibacterial activity against E. coli (38.00± 0.61 mm, Figure 8a) in 100 µg/ml and
    the lowest antibacterial activity against S. aureus (11.00± 0.41 mm, Figure 8a) in
    25 µg/ml. Silver nitrate solution shows the highest antibacterial activity against E. coli (28.00±0.41
    mm, Figure 8b) in 100 µg/ml and the lowest antibacterial

    DOI: 10.9790/3008- g 1 | Page


    IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)

    activity against S. aureus (9.00 ± 0.58 mm, Figure 8b) in 25 µg/ml. When antibacterial
    activity of silver nitrate and AgNPs are compared against the studied bacteria, an increase in
    antibacterial activity of AgNPs over the use of silver nitrate is observed. Similarly, it has been
    reported that AgNPs show higher antibacterial activity than silver nitrate (Marslin et al.,
    2015).
    The high surface area to volume ratio of AgNPs increases their contact with micro-
    organisms, promoting the dissolution of Ag + ions and hence improving biocidal effectiveness.
    Formation of free radicals by the AgNPs when in contact with the bacteria, and free radicals
    have the ability to damage the cell membrane and make it porous which can ultimately lead
    to cell death (Tenzin et al., 2016). Silver is a soft acid, and there is a natural tendency of an
    acid to react with a base; in this case, a soft acid to react with a soft base. Another fact is
    that the deoxyribonucleic acid (DNA) has sulfur and phosphorus as its major components;
    AgNPs can act on these soft bases and destroy the DNA which would definitely lead to cell
    death (Masoud et al., 2016).

    DOI: 10.9790/3008- www.iosrjournals.or 2 | Page

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