HPLC

Detector Range
Who is GBC Scientific Equipment? ISO 9001 QUALITY
ACCREDITATION
GBC Scientific Equipment Pty Ltd thinking and an obsession for quality GBC has always placed a
commenced operations in 1978. and reliability. GBC now produces strong emphasis on quality in
GBC designs, manufactures and the widest range of AA spectrometers all aspects of our operation,
markets a range of scientific in the world. from design and manufacture to
instruments comprising quality the provision of service and
analytical Atomic Absorption Endorsed by the international quality support to our customers, and
spectrometers (AAS), UV-Visible standard, ISO 9001, the company we are fully committed to
prides itself on developing products
continuous evaluation and
to exceed market expectations.
improvement in all areas.
GBC is the proud recipient of many
international and export awards for The GBC Quality Management
state-of-the-art technology and System has been accredited to
design throughout the world. the ISO 9001 quality standard
by Lloyd’s Register Quality
The company is proudly Australian Assurance Limited. This
and Head Office is based in certification is your assurance
Melbourne. The GBC network spans that the procedures and
all sectors of the globe. Now more processes used to produce the
than 25 years after its inception, goods and services which GBC
GBC is renowned as being provides comply with the
progressive in the elemental and relevant International
spectrometers (UV-VIS), Inductively materials analysis fields.
Coupled Plasma Optical Emission Standard, and demonstrates
spectrometers (ICP-OES), Inductively our commitment to meeting the
GBC customers benefit from an
Coupled Plasma orthogonal Time of needs and the expectations of
efficient and effective global
Flight Mass spectrometers our customers.
organization. Access to information,
(ICP-oTOFMS), High Performance applications support and technical
Liquid Chromatographs (HPLC), service is never more than a phone
Fastest Viscoelastic Biomechanical call or email away.
Rheometry and XRD instruments and
upgrades.
GBC's growth has been fuelled by its
extensive AA expertise, innovative

What is the GBC vision?

GBC Scientific Equipment
will
advance people’s knowledge

and
their capacity to enhance the quality of life

for all humankind.

GBC’s product lines...

AAS HPLC ICP-OES ICP-oTOFMS Rheometer UV-Vis XRD

LC 1200 Variable Wavelength UV-V
Vis Detector
GBC offers a comprehensive range of quality
high performance HPLC detectors from turn-key
systems to stand alone modules including
autosamplers, pumps and detectors. GBC also
offers accessories such as columns, fittings and
tool kits.

Simplify your choices by selecting one of our

customised and complete HPLC Systems or
choose from our comprehensive range of pumps,
detectors, and autosamplers to compliment your
existing system.

LC1200 Variable Wavelength UV-Vis Detector

LC 1200 Variable Wavelength Drift Analog Outputs

UV-V
Vis Detector < 5 x 10-4 AU/hour after warm-up Two outputs (Integrator and Chart Recorder)
at 254 nm Integrator output
Flexible operation at single wavelengths 1.0 AU/V (unattenuated)
within the range of 190-600 nm makes Wavelength Accuracy Chart Recorder Output:
for versatility. For example, rather than ±2 nm 10 mV FS (attenuated)
being restricted to one or two
wavelengths, you can use the LC1200 Spectral Bandwidth Diagnostics
at 254 nm for the detection of aromatic 10 nm Reference energy and sample energy.
compounds, improve sensitivity and
selectivity for proteins, peptides, Absorbance Range Operating Environment
phenols and catecholamines at 280 .001 - 2.0 AUFS 4 to 40ºC, < 85% relative humdity
nm, detect analytes of interest in the
visible range, or monitor nitrate and Absorbance Linearity Power Requirements
carboxylic acids at 200 nm. ±1% to 1.0 AU at 254 nm 100 -120/220-240V, 50-60 Hz

Optical Design Time Constant Dimensions

User-selectable from 0.1, 0.2, 0.5 sec 263 x 183 x 450 mm (W x H x D).
High energy forward optical design with
concave holographic grating Lamp Weight
monochromator. Deuterium (pre-aligned) Nett 13.2 kg, Shipping 16.2 kg

Wavelength Range Flowcell

UV and Visible (190 - 600 nm) 10 mm pathlength with 10µl volume
Pressure Limit: 7 MPa (1029 psi)
Noise
Display
<± 2 x 10-5 AU at 254 NM,
0.1 sec time constant. Liquid crystal display
Displayed Parameters:Operating
Wavelength, Reference Energy,
Sample Energy and Absorbance.

Turn-key Systems or Stand Alone Modules

3
LC1205K programmable and LC1210K dual wavelength UV-V
Vis Detectors
Digital Output Digital Output
RS-232 connector 2 x RS-232 connectors

LC1205K
Analog Output Analog Output
UV-V
Vis Detector ± 1V (TTL, OC, relays) signal output for
fraction collector
Dimensions Integrator output:±0.1V/±1V/ ±10V
106 x 185 x 340 mm. (W x H x D)
Dimensions
160 x 185 x 340 mm (W x H x D)
LC1210K UV-V
Vis Detector
LC1205K UV-V
Vis Detector The units are now lighter and much
The LC1210K UV-Vis Detector can smaller in size. The LC1205K and
The LC1205K is a programmable simultaneously acquire multiple LC1210K have improved with regards to
variable wavelength UV-Vis detector and chromatograms at different drift and noise. The electronics and the
can operate with interchangeable flow wavelengths. unique optical alignment result in high
cells for micro, analytical and throughput light and sampling. The
preparative HPLC. The wavelength can The LC1210K offers two adjustable specially designed capillary flow cells
be set between 190 nm and 740 nm wavelengths per run in the stand-alone make the LC1205K and LC1210K
for a run and can also be changed mode whereas four wavelengths are detectors the most sensitive in the market
during the measurement. This makes available with external control. UV place. Some features include fast
programming various wavelengths for spectra can be acquired in the on-flow scanning of 100 nm/s for spectral
different runs easy. To acquire UV- mode. acquisition, simultaneous detection of up
spectra the detector is switched to the to two wavelengths, analogue output of
stop-flow mode. Wavelength Range up to two signals, ratio plots, low noise
190 - 740 nm level and an open interface protocol for
Wavelength Range Edge Filter (2nd order) 370 nm remote instrument control with other
190 - 740 nm software.
Edge Filter (2nd order) 370 nm Light Source:
Deuterium lamp, optional tungsten - The LC1205K and LC1210K detectors
Light Source: halogen lamp have no confined flow cell compartment.
Deuterium lamp, Optional tungsten - The flow cell is simply installed on a rail
halogen lamp Band Width/Wavelength Accuracy: at the front of the detector. This allows
∆λ 8nm /±1nm the use of flow cells with much longer
Band Width/Wavelength Accuracy: path lengths than in conventional
∆λ 8nm /±2nm Drift: arrangements as well as for easy, quick
<1 x 10-4 AU/h at 240 nm flow cell exchange. The absence of any
Drift: dead volume and the superior flushing
<15 x 10-5 AU/h at 254 nm Noise: characteristics of the flow cell fully
<1 x 10-5 AU/h at 254 nm, t=1s preserve the chromatographic resolution.
Noise: With fibre optics:<8 x 10-5 AU/h at 254 A variety of flow cells are available.
<1 x 10-5 AU/h at 254 nm, t=1s nm, t=ls

Sensitivity: Sensitivity:
2 x 10-5 AU/h at 254 nm, t=1s <2 x 10-5 AU/h at 254 nm, t=1s
LC1210K
Vis Detector
UV-V
Scan Speed: Scan Speed:
Complete wavelength range on-flow Complete wavelength range on-flow
100nm/s 100 nm/s

Sophisticated and Easy to Use

4
LC1255s Fluorescence Detector and LC1245K Refractive Index Detector

LC1255s Fluorescence Detector LC1245K Refractive Index Detector

GBC’c new LC1255s fluorescence The refractive index detector is suitable

detector comes with new”DC arc High for detecting little or no UV active
Pressure Xenon Lamp” technology compunds such as sugars or polymers.
offering significant improvements over With a user-friendly keypad and LCD
traditional “Xenon Flash” tube design of display, the LC1245K continuously
the old LC1255. The new detector monitors signal output and provides
offers a refined design for the ultimate immediate visual feedback on detector
in high sensitivity detection. The performance and operation. For
LC1255s offers an improved Raman LC1255s Fluorescence Detector unattended operation, all detector
signal-to-noise ratio of 300 for controls can be interfaced with your
excellent sensitivity, improved data station through relay contacts.
wavelength accuracy of ± 2 nm for Sensitivity
strong between-unit consistency and a The S/N ratio is 300 for the Raman line Wavlength
newly developed ratio system which of distilled water (350 nm excitation 950 nm ± 30 nm
compensates for lamp intensity wavelength and 1.5 sec. time constant.
fluctuation and provides superb noise Measuring Range
characteristics. The LC1255s features Detector: ± 1 x 10-3 ∆n
other enhancements including a new Photomultiplier
remote lamp on-off function to allow Sensitivity
lamp shutdown upon run completion, Cell (volume, pressure, material):
<8 x 10-8 ∆n
and a new rugged flow cell design with 12 µl, approximately 20kgf/cm2
elevated back-pressure tolerance. This (2Mpa) SUS316, Quartz, PTFE, as Noise
offers the user more effective and standard (2µl cell and insert cells are <4 x 10-8 ∆n
durable operation.
available as options)
Cell Angle
Light Source:
High Precision Xenon Lamp 150W 45º
Wavelength scanning:
Possible for both excitation and Cell Volume
Excitation/Emission monochromaters:
Concave, blaze holographic grating emission wavelengths. 15 µl
monochromators, F 2.4
Maximum Flow Range
Time programming:
Measuring wavelength range: Maximum 5 ml/min
Wavelength parameters may be
200-600 nm ( 200-900 nm option)
programmed in up to 32 steps Autozero
Spectral Bandwidth: Full range
15 nm both in excitation and emission Dimensions:
sides Analog Output
260 x 205 x 520 mm (W x H x D) ± 1V analog, RS-
Wavelength accuracy: 232 connector,
±2 nm Weight: remote connection
17 kgs
Wavelength reproducibility Dimensions
±0.2 nm 106 x 185 x 340 mm
(W x H x D)
LC1245K Refractive
Weight: 8.0 kg Index Detector

High Sensitivity Detection

5
LC1260 Electrochemical Detector and LC1275 Conductivity Detector

Output: Analog - 10mV, 100mV, 1V

Full-Scale voltage

Remote Auto-zzero: Contact Closure

Computer Interface: RS-232 Serial

Communication

Power: 120/240 VAC 50/60 HZ, 100

LC1260 Electrochemical Detector LC1275 Conductivity Detector Watts (Field Selectable)

LC1260 Electrochemical Detector LC 1275 Conductivity Detector Cell Compartment Size:

57.2 x 57.2 x 158.8 mm (W x H x D)
The LC1260 electrochemical detector The LC1275 is a high performance
provides enhanced detector conductivity detector for the rapid Insulation:Minimum 1/2” all sides
performance in a robust and easy to determination of anions, cations, metals,
use instrument. It adopts a patented organic acids and surfactants down to Heater: 25 W DC heating element
Wall Jet Flowcell design optimised for ppb levels. vulcanized to aluminium block
high assay sensitivity, wide linear
dynamic range, response stability and A temperature stabilised flow cell is Cell Temp: Factory set at 35ºC, User
reproducibility. In LCEC the electrode isolated from external temperatures adjustable ambient to 60ºC
surface is in direct contact with the test fluctuation, and active temperature
medium, resulting in gradual fouling of control of the detector eliminates Temp. Stability: ±0.05ºC
the electrode surface due to the temperature induced variations in
absorption of chemicals. The washing analyte or background signals. Cell Type: 2 gold-plated SS electrodes
effect of the impinging jet made
possible by the wall jet design improves The LC1275 is suitable for both Cell Volume: 0.5
electrode stability and precision with suppressor based and single column IC
much-reduced down-time for electrode applications. The detector auto-zero Pressure Rating: 400 psig
cleaning. can offset high conductivity mobile
phase for sensitive detection of ions, GBC Auto Suppressor
Detector Design essential in single column applications.
High sensitivity amperometric open wall The GBC auto suppressor is a
jet flowcell design with integrated cell Detection: Alternating Current, continuous, reagent free solid-phase
compartment. Synchronous Detection 10kHz Frequency suppressor for ion chromatography. In
addition to reducing background
Operating Modes Range Display: 0.01 - 5000 s in 12 conductance and enhancing analyte
DC and Pulse steps signal, the GBC auto suppressor
Auto-zero: Offsets up to 10,000 s removes carbonic acid before entering
Working Potential the detector. This enables powerful
± 2.000 V in 1 mV increments Time Constant: 0.1, 0.5, 1, 5, 10 carbonate/bicarbonate gradients and
seconds, software selectable increases sensitivity by 20%.
Pulse Cleaning Potential Method Storage: 0-10 user defined Continuous operation and solid -
± 2.000 V in 1 mV increments phase chemistry eliminates reagents,
Size: 262 x 482 x 117 mm (W x D X H) swithing valves, down time and
Current Range cumbersome multiple operating modes.
10pA to 5000 nA FS (Recorder Output) Weight: 17lbs (5.9kg)
(15 steps in1,2,5, sequence).
1 nA to 1000 nA FS (Integrator Output)
(4 steps in decade sequence).

Wide Linear Dynamic Range

6
LC5100 Photo-D
Diode Array Detector

The LC5100 Photo-D

Diode Array The LC5100 is very easy to use, with Dimensions
Detector the light sources and flow cell located 260 x 420 x 140 mm (W x D x H)
at the front of the instrument. A leak
The LC5100 is a very high performance sensor alerts the user to any solvent Weight
photodiode array detector for HPLC. leak and an interlock switch protects the 10.5 kg
Precision optics ensures high sensitivity, user during lamp replacement when the
superior spectral resolution as well as protection cover is removed. These Safety
high stability. features increase operational safety. Leak sensor

This very compact PDA provides the The WinChrom Chromatography Data Automatic lamps shut off when lamp
latest validation tools to support System software package Version 1.32 cover is removed.
GLP/GMP. The LC5100 delivers a provides complete instrument control.
superb noise level of ±0.8 x 10-5 AU for Thermostatted at lamp housing surface
unsurpassed sensitivity, bringing new Wavelength Range temperature fuse at power supply
levels of accuracy to trace component 190-800 nm
identification, spectral elucidation and Ease of Use
library searching. Full scan spectra can Light Source Light sources and cell are pre aligned
be continuously collected in the flowing Deuterium on, Tungsten-Halogen on or and accessible from front of instrument
analyte stream both on
OQ
Diodes Instrument performs automatic
Single array, 512 photodiodes wavelength accuracy function at four
wavelengths upon power up
Wavelength Accuracy
1.0 nm

Resolution
Spectral Range nm/diode
190 - 800 nm 1.2
LC5100 Photo-Diode Array detector
Noise
± 0.8 x 10-5 AU (250 nm, 600 nm)
The detector is equipped with both a
deuterium lamp and a tungsten- Baseline Drift
halogen lamp to provide superior < 1 x 10-3 AU/hr
sensitivity over the 190 - 800 nm range
and provide real time spectra using a Flow Cells
512 element photodiode array. Full 10 mm pathlength, (standard)
spectral information can be provided at
any time during the chromatographic Maximum Pressure
run. Chromatograms can be generated 12MPa
at individual wavelengths or as three-
dimensional plots showing absorbance AC Power
against both time and wavelength. An 100/120/220/240 Volts, 50/60 Hz,
automatic wavelength accuracy check is 150 watts
performed at four wavelengths as part
of the validation procedure.

Superior Spectral Resolution

7
 Designed and manufactured by
Ordering Information GBC Scientific Equipment Pty Ltd
A.C.N. 005 472 686
Part Number Description GBC reserves the right to change
specifications without prior notice.
GBC publication number
100-121700 LC1200 Variable Wavelength UV-Vis Detector
01-0815-02
99-0517-00 LC1205K Programmable UV-Vis Detector April 2003 Australia
99-0518-00 LC1210K Dual Wavelength UV-Vis Detector
GBC SCIENTIFIC EQUIPMENT
99-0533-00 LC1245K Refractive Index Detector
Manufacturer of world-class scientific
99-1485-00 LC1255s Fluorescence Detector instruments and accessories -
99-0272-00 LC1260 Electrochemical Detector AA, HPLC, ICP-OES, ICP-oTOFMS,
100-1275-00 LC1275 Conductivity Detector Rheometry, UV-Vis and XRD
99-1472-01 LC5100 Photo-diode Array Detector 220V 12 Monterey Road
99-1473-01 LC5100 Photo-diode Array Detector 110V Dandenong, Victoria 3175
Australia

Telephone 61 3 9213 3666

Facsimile 61 3 9213 3677
email gbc@gbcsci.com
URL www.gbcsci.com
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are the property of their respective
owners.