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    Exercise 12

    Uploaded by

    Bhumika Mahalik
    This document provides instructions for performing controlled pollination through emasculation. It describes the process as: (1) Selecting a flower bud before anthesis and removing the stamens to produce a female parent plant. (2) Covering the emasculated flower with a bag to protect it from unwanted pollen during bagging. (3) Transferring pollen from the desired male parent plant directly onto the stigma of the emasculated female flower. The goal is to control which parental plants are used to produce seeds with desired traits through human manipulation of the reproductive process.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Exercise 12

    Uploaded by

    Bhumika Mahalik
    59 views2 pages
    This document provides instructions for performing controlled pollination through emasculation. It describes the process as: (1) Selecting a flower bud before anthesis and removing the stamens to produce a female parent plant. (2) Covering the emasculated flower with a bag to protect it from unwanted pollen during bagging. (3) Transferring pollen from the desired male parent plant directly onto the stigma of the emasculated female flower. The goal is to control which parental plants are used to produce seeds with desired traits through human manipulation of the reproductive process.

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    © © All Rights Reserved

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    This document provides instructions for performing controlled pollination through emasculation. It describes the process as: (1) Selecting a flower bud before anthesis and removing the stamens to produce a female parent plant. (2) Covering the emasculated flower with a bag to protect it from unwanted pollen during bagging. (3) Transferring pollen from the desired male parent plant directly onto the stigma of the emasculated female flower. The goal is to control which parental plants are used to produce seeds with desired traits through human manipulation of the reproductive process.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    59 views2 pages

    Exercise 12

    Uploaded by

    Bhumika Mahalik
    This document provides instructions for performing controlled pollination through emasculation. It describes the process as: (1) Selecting a flower bud before anthesis and removing the stamens to produce a female parent plant. (2) Covering the emasculated flower with a bag to protect it from unwanted pollen during bagging. (3) Transferring pollen from the desired male parent plant directly onto the stigma of the emasculated female flower. The goal is to control which parental plants are used to produce seeds with desired traits through human manipulation of the reproductive process.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    Exercise 12

    

    
    
    
    
    
    
    

    
    

    Procedure
    (i) Select a flower in bud condition where antheses has not occurred. Open
    the bud carefully and remove the stamens (Fig. 12.1). Mark this as
    female parent plant.

    Fig. 12.1 Showing process of Emasculation

    21/04/2018
    LABORATORY MANUAL: BIOLOGY

    (ii) Cover the emasculated flower with a plastic


    bag to protect it from undesired pollen
    (Bagging) (Fig. 12.2). The bag should be
    held securely in place with a paper clip/
    string/thread. Select the size of bag in
    accordance with the flower size. Bags
    must be transparent with minute pores.

    Fig. 12.2 Bagging of an emasculated flower

    (iii) Bring into physical contact anthers of a


    desired male plant containing mature
    pollen grains with the stigmatic surface
    of emasculated female flower (Fig. 12.3).
    Use tweezers/brush if necessary to dust
    the stigmatic surface with pollen.

    (iv) Cover the pollinated flower again with the


    bag immediately. For identification, label
    the female parent (Tagging). Each
    pollinated flower should bear a label
    Fig. 12.3 Showing cross pollination on an containing the name of the seed parent,
    emasculated flower the letter X (to signify a cross), the name
    of the pollen parent, and the date on which
    the cross was effected.

    Questions
    1. Why is emasculation performed before anthesis?
    2. What are the advantages of using a bag containing minute pores?

    46

    21/04/2018

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