PAPER BIOCHEMSTRY

A) Select the most appropriate option. Each question contain one (1)
marks.

1) The starch is made up of

a. α-D Glucose
b. β-D Glucose
c. α-L Glucose
d. β-L Glucose

2) In amylose unit the glycosidic linkage is

a. 1 → 2
b. 1 → 3
c. 1 → 4
d. 1 → 6

3) Which one of the following has the unbranched structure

a. Starch
b. Cellulose
c. Glycogen
d. Both a & b

4) Which one of the following is called grapes sugar

a. Glucose
b. Fructose
c. Mannose
d. Maltose

5) GAGs are special type of polysaccharide that consists of repeating

disaccharide unit bind with each other through
a. 1 → 4 linkage
b. 1 → 6 linkage
c. 1 → 2 linkage
d. 1 → 3 linkage
6) Heparin is Naturally …………
a. Antiseptic
b. Anticoagulant
c. Coagulant
d. Antibiotic

7) In case of Glucoronic Acid the oxidation takes place at

a. Carbon # 1
b. Carbon # 6
c. Carbon # 2
d. Both a & b

8) In Amino sugars like Glucosamine the Amino group –NH2 group is

attached to Carbon #........
a. 1
b. 2
c. 4
d. 6

9) All are Aldohexose except

a. Glucose
b. Galactose
c. Mannose
d. Fructose

10) C5H10O5 is the chemical formula of

a. Ribose
b. Erythrose
c. Ribulose
d. Both a & c
11) Actin and myosin function is
a) Transportation
b) Regulation
c) Relaxation
d) Contraction

12) Muscle glycogen doesnot directly contribute to blood glucose due

to the absence of
 a. Glucose phosphatase
b. Glucose synthetase
c. Glucose hydrogenase
d. None of the above

13) The amino acids which take part in the formation of glucose is
called
a. Ketogenic amino acids
b. Glucogenic amino acid
c. Standard aminoacids
d. Both a and b

14) The name of the protein that has been identified to serve as a
primer of glycogen synthesis is____________
a. Ubiquinone
b. Insuline
c. Glycogenin
d. All of the above

15) Hyperglycemia stands for

a. Decrease in blood glucose level
b. Decrease in blood glycogen level
c. Increase in blood glucose level
d. Increase in glycogen level

16) Kinase enzyme does__________

a. Isomerization
b. Hydrolysis
c. Reduction
d. Phosphorylation

17) In anaerobic respiration the end product of glucose metabolism is

a. Pyruvic acid
b. Acetyl CoA
c. Oxaloacetic acid
d. Lactic acid

18) Which one of the following is keto sugar

a. Dihydroxy aceton
 b. Fructose
c. Glucose
d. Both a and b
19) Sucrose is a disaccharide having two molecule of
a. Glucose and fructose
b. Glucose and glucose
c. Glucose and galactose
d. Glucose and manose

20) ATP contain which monosaccharide in its structure

a) Ribose
b) Ribulose
c) Deoxyribose
d) Galactose
21) Fructose is present in
a) Human blood
b) Seminal fluid
c) Synoval fluid
d) None of the above
22) The pair of sterioisomers that are mirror image of each othersin
regard to asymmetric carbon are called
a. Anomers
b. Epimers
c. Enantiomers
d. Optical isomers
23) Which one of the following is the example of heteropolysaccharide
a. Cellulose
b. Starch
c. Hyaluronic acid
d. All of the above

24) Dextrorotatory means

a. Rotation of plane-polarized light towards left
b. Rotation of plane-polarized light towards right
c. Rotation of plane-plarized light towards up
d. Rotation of plane-polarized light towards down
25) A pentose sugar is
a. Dihydroxyacetone
 b. Ribulose
c. Erythrose
d. Glucose

26) The sugar found in RNA is

a. Ribose
b. Deoxyribose
c. Ribulose
d. Erythrose

27) A polysaccharide which is often called animal starch is

a. Glycogen
b. Starch
c. Inulin
d. Dextrin

28) A carbohydrate found in DNA is

a. Ribose
b. Deoxyribose
c. Ribulose
d. All of these

29) Glucose-6-phosphatase is not present in

a. Liver and kidneys
b. Kidneys and muscles
c. Kidneys and adipose tissue
d. Muscles and adipose tissue

30) Gluconeogenesis is decreased by

a. Glucagon
 b. Epinephrine
c. Glucocorticoids
d. Insulin

31) Cori’s cycle transfers

a. Glucose from muscles to liver
b. Lactate from muscles to liver
c. Lactate from liver to muscles
d. Pyruvate from liver to muscles

32) Dihydroxyacetone phosphate and glyceraldehyde-3-phosphate are interconverted

by
a. Triose isomerase
b. Phosphotriose isomerase
c. Diphosphotriose isomerase
d. Dihydroxyacetone phosphorylase

33) The carrier of the citric acid cycle is

a. Succinate
b. Fumarate
c. Malate
d. Oxaloacetate

34) The conversion of alanine to glucose is termed

a. Glycolysis
b. Oxidative decarboxylation
c. Specific dynamic action
d. Gluconeogenesis

35) The following is an enzyme required for glycolysis

a. Pyruvate kinase
 b. Pyruvate carboxylase
c. Glucose-6-phosphatase
d. Glycerokinase

36) Conversion of glucose to glucose-6-phosphate in human liver is by

a. Hexokinase only
b. Glucokinase only
c. Hexokinase and glucokinase
d. Glucose-6-phosphate dehydrogenase

37) During glycolysis, Fructose 1, 6 bisphosphate is cleaved by the enzyme:

a. Enolase
b. Fructokinase
c. Aldolase
d. Diphosphofructophosphate

38) Which of the following is not an enzyme involved in glycolysis

a. Euolase
b. Aldolase
c. Hexokinase
d. Glucose oxidase

39) Dehydrogenation of succinic acid to fumaric acid requires the following

hydrogen carrier:
a. NAD+
b. NADP+
c. Flavoprotein
d. Glutathione

40) The number of molecules of ATP produced by the total oxidation of acetyl CoA
in TCA cycle is
 a. 6
b. 8
c. 10
d. 20

41) When O2 supply is inediquate, pyruvate is converted to

a. Phosphopyruvate
b. Acetyle CoA
c. Lactate
d. Alanine

42) Which of the following is a rate limiting enzyme of gluconeogenesis?

a. Hexokinase
b. Phosphofructokinase
c. Pyruvate carboxylase
d. Pyruvate kinase

43) The enzymes involved in the phosphorylation of glucose to glucose-6-phosphate

are
a. Hexokinase
b. Glucokinase
c. Phosphofructokinase
d. Both a & b

44) The enzyme of glycolysis in eukaryotic cells are located in the

a. Mitochondrial matrix
b. Cytosol
c. Plasma membrane
d. Intermembrane space
45) How many molecules of ATPsare synthesized per NADH
oxidation
a. 1.5
b. 2
c. 2.5
 d. None of the above

B) Answer the following. Each question contain Five (5) marks.

1) Classification of Carbohydrates. (5)

2) Discuss Glycogen under following headings
a) Nature, b) Occurance, c) Function d) Structure
3) Write a detail note on Table sugar. (5)
4) Discuss the concept of D&L Monosaccharide.(3)
Explain by drawing the structure and high light the carbon atom
due to which the monosaccharide is given D or L name. (2)
5) Discuss the importance of Cellulose in Human Nutrition. (5)
6) Define glycolysis? (1)
Write a detail note on glycolysis. (4)
7) Differentiate between Glycogenolysis and Gluconeogenesis. (1)
Discuss the factors, effects the tissue content of glycogen. (4)
8) Draw a krebs cycle. (2.5)
Calculate the number of ATPs synthesized in complete oxidation of one mole
of Glucose. (2.5)
9) Write a note on Fate of Pyruvic Acid (5)
 TEST (B) BIOCHEMISTRY
A) Select the most appropriate option. Each question contain one
(1) marks. (10)
1) In amylopectin unit, linkage are
a. 1 → 2 & 1 → 4
b. 1 → 3 & 1 → 4
c. 1 → 4 & 1 → 5
d. 1 → 4 & 1 → 6

2) Cellulose has
a. β – D – Glucopyranose
b. β – L – Glucopyranose
c. α - D – Glucopyranose
d. β – D – Glucofuranose

3) The combination of large number of glucose unit give rise to

a. Starch
b. Cellulose
c. Glycogen
d. All of the above

4) Which one is the principle monosaccharide used by the tissue

obtaining energy
a. Fructose
b. Galactose
c. Lactose
d. Glucose

5) Which one of the following is called fruit sugar

a. Glucose
b. Fructose
c. Lactose
 d. Mannose

6) ………….. is the more Electronegative

a. Heparin
b. Condritin sulfate
c. Darmatin sulfate
d. Hyaluronic acid
7) All GAGs occurs in combination with extracellular proteins
through covalent bonds forming Proteoglycan except
a. Heparin
b. Condritin sulfate
c. Hyaluronic acid
d. Keratin sulfate

8) Immunoglobulins are …………… in nature

a. Proteoglycans
b. Glycoproteins
c. Glycoaminoglycans
d. Glycolipids

9) Which one of the following has the unbranched structure

a. Cellulose
b. Glycogen
c. Starch
d. Both b & c

10) All are ketosugars except

a. Dihydroxyacetone
b. Ribulose
c. Glyceraldehyde
d. Fructose

B) Write a short note of the following. Each question contain two

(2) marks.
(10)
1) Why Sucrose is a non-reducing sugar? but when it is hydrolyzed it
shows reducing property, WHY?
2) GAGs are strong Electronegative in nature, WHY? Give the
example of most electronegative GAG.
3) Glucose when react with phenylhydrazine give glucosazone.
Fructose & Mannose also give glucosazone, WHY?
4) Differentiate between Cellulose & Cellubiose.
5) Differentiate between Homo & HETERO polysaccharide with
example.

C) Answer the following. Each question contain Five (4) marks.

1) Hyaluronidase?
a) Nature, b) Occurance, c) Biological activity
2) Discuss Starch under following headings:
a) Nature, b) Occurance, c) Function, d) Structure
3) What happened when there is 1 → 4 linkage &1 → 6 linkage
between monosaccharide. Draw the structure which the linkage.
4) Write a detail note on Grape Sugar.
5) Optical isomerism.
6) Discuss Pentose as:
a) Structure, b) Aldose & Ketose, c) Properties

D) Remarks for the Test is compulsory. It contain TWO (2) marks

 TEST (C) BIOCHEMSTRY

E) Select the most appropriate option. Each question contain one

(1) marks. (10)

1) Lactose is disaccharide synthesized by two monosaccharide bind

through
a. α – 1 → 4 linkage
b. β – 1 → 4 linkage
c. α – 1 → 6 linkage
d. β – 1 → 6 linkage

2) ATP contain which monosaccharide in its structure

a. Ribulose
b. Ribose
c. Deoxribose
d. Galactose
3) Fructose is present in appreciated amount in
a. Seminal fluid
b. Synovial fluid
c. Human blood
d. None of the above

4) Cellulose has
 a. β – D – Glucofuranose
b. β – L – Glucopyranose
c. α - D – Glucopyranose
d. β – D – Glucopyranose

5) GAGs are special type of polysaccharide that consists of repeating

disaccharide unit in which the monosaccharide bind with each
other through
a. 1 → 4 linkage
b. 1 → 6 linkage
c. 1 → 3 linkage
d. None of the above

6) Maltose has
a. Glucose & Fructose
b. Galactose & Glucose
c. Glucose & Glucose
d. Glucose & Mannose

7) In Amino sugars like Glucosamine the Amino group –NH2 group

is attached to Carbon #........
a. 1
b. 2
c. 4
d. 6

8) Complete the fermentation reaction of Glucose

C6 H12O6

9) Glucose reduced to ………. & Mannose reduced to………..

a. Sorbitol & Mannitol
b. Mannitol & Sorbitol
c. Sorbitol & Rabitol
d. Mannitol & Rabitol

10) Agar is non-digestible and if ingested it

a. Stimulate the immunity
b. Decrease the intestinal peristalsis
c. Stimulate the intestinal peristalsis
 d. Decrease the immunity

F) Write a short note of the following. Each question contain two

(2) marks. (10)

1) Sucrose is dextrorotatory. On hydrolysis it become levorotatory,

WHY?
2) Freshly prepared solution of alpha-D glucose or beta-D glucose
shows Mutarotatin. WHY?
3) Cellulose cannot be digested by human body. But it is Nutritionally
very important & should be taken in diet. WHY?
4) Why starch does not act as a reducing sugar.
5) Racemic mixture contain optically active compounds but NO net
rotation of plane-polarized light. WHY?

G) Answer the following. Each question contain Five (5) marks.

1) Write a detail note on Fruit Sugar.

2) Discuss Cellulose under following headings:
a) Nature, b) Occurance, c) Function, d) Structure
3) Write down the generic name, aldose and ketose of 3,4,5 & 6
Carbon containing Monosaccharide.
4) What happened when there is 1 → 4 linkage &1 → 6 linkage
between monosaccharide. Draw the structure which the linkage.
5) What is GAGs? Examples? Explain atleast one in detail
6) Discuss Glycoprotein? Different compound are present inside are
glycoprotein in nature. Write the name of those glycoprotein and
mentioned there biological role.

H) Remarks for the Test is compulsory. It contain TWO (2) marks