MICROBE MISSION SAMPLE 2

PART 1: Microscopy (You are given a microscope with a 10 X ocular and 4, 10, and 40 X objectives.)

1. What is the highest magnification possible with this microscope?

2. What is the range of magnification for this microscope?

3. A student prepares a slide with the letter "d" and positions it on the stage in the normal reading position.
When viewed, how will the "d" will appear? Draw it on your answer sheet.

4. Which part of this microscope is used to control the amount of light coming to the image from the light
source?
(A) objective (B) diaphram (C) ocular (D) fine adjustment

5. What part of this microscope holds and rotates the objectives?

(A) stage (B) body tube (C) coarse adjustment (D) nosepiece (E) fine adjustment knob

6. When observing an object under low power and then switching to high power, the image under
high power will appear
(A.) smaller and brighter (C.) smaller and darker
(B.) larger and brighter (D.) larger and darker

7. Using the hands of the clock to describe places on the field of view, if a object appears to be moving
toward 1 o’clock, what direction is it actually moving?

8. Examine the diagram of the stage with millimeter ruler from a 10X objective. What is the diameter of
this field of view (the diagram) in millimeters? in micrometers or mcm?

9. Examine the Photo of Microbe A taken under low power. What type of microbe is Microbe A?
(virus, protozoa, algae, fungi, bacteria)

10. What is the approximate length of one of Microbe A cells that is not reproducing in micrometers (mcm)?

Diagram of stage with

millimeter ruler (10X objective) Microbe A (10X objective)

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PART 2: Types of Microscopes

Key: A. Light microscope
B. Phase Contrast Microscope
C. Florescent Microscope
D. Scanning Electron Microscope
E. Transmission Electron Microscope
Use the above key to identify the type of microscope being described in questions 11-15.
11. Produces extremely detailed 3-D images.
12. Produces very detailed 2-D images of very thin stained specimens.
13. Can observe live specimens in normal color and background as they move about a slide.
14. Can observe live specimens contrasted against a gray background.
15. Uses dyes to show florescence under UV rays?
Use images A-E in answering questions 16-20. Each image is of yeast taken from a different type
of microscope.
16. Which image is from a light microscope?
17. Which image is from a phase contrast microscope?
18. Which image is from a florescent microscope?
19. Which image is from a scanning transmission microscope?
20. Which image is from a transmission electron microscope?

A B C

D E
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PART 3: Relative Size of Microbes
21. How many micrometers (mcm) are in a millimeter (mm)?
22. How many nanometers (nm) are in a micrometer (cmc)?
23. How many nanometers (nm) are in a millimeter (mm)?
Use the microbe images for questions 24-30.
24. What is the size of a Volvox in nanometers?
25. What is the size of a yeast in nanometers?
26. What is the size of a halobacterium in nanometers?

27. What is the size of a coccus in nanometers?

28. How many viruses would fit across the diameter of a coccus?

29. How many viruses would fit across the diameter of a yeast?

30. How many viruses would fit across the diameter of a Volvox?

diatom-200 mcm Volvox-800 mcm

yeast-10 mcm halobacterium – 2 mcm

Coccus – 1 mcm Virus – 50 nm

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PART 4: Cell Types

Use Diagrams A, B, and C to help you answer the following questions

31. Which diagram (A,B,or C) is acellular?

32. Which diagram (A,B,or C) is a prokaryotic cell?
33. Which diagram (A,B,or C) is a eukaryotic cell?

For questions 34-39, indicate which cell type (A, B, or C) would fit each of the following microbes
34. fungi
35. virus
36. algae (Plant-like protist)
37. bacteria
38. protozoa (Animal-like protest)
39. Archaea
40. Which of the following would be found in all three diagrams?
A. endoplasmic reticulum
B. ribosomes
C. vacuole
D. DNA or RNA

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PART 5: Major Groups of Microbes

A. Prion
B. Archeae
C. Bacteria
D. Fungi
E. Algae (Plant-like protists)
F. Protozoa (Animal-like protists)
G. Virus
For questions 41-50, use the above key to identify the appropriate group of microbes being
described.
41. Important zooplankton

42. Have cell walls and chloroplasts – primary producers in many aquatic environments

43. Acellular cluster of DNA or RNA encased in a protein - many types cause diseases

44. Proteinaceous infectious particles – cause disease

45. Yeast are examples of these microbes

46. Some species are extremophiles – living in extremes of pH, temperature, salinity as well as heat,
acid and toxic gases

47. Organized by mode of transportation as cilia, flagella, pseudopods

48. Many are edible and cultivated world wide – others are major decomposers in the soil

49. Cause the flu and the common cold

50. Prokaryotic rods, spheres, or spirals- some can cause disease

PART 6: Microbes and Food

For questions 51-55, use the key of microbes to identify the microbe used in making the following
foods – some foods may include more than one microbe type.
Key: A. yeast B. Bacteria C. mushrooms D. Algae
51. Yogurt
52. Bread
53. Cheese
54. Wine
55. Pepperoni Pizza

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 MICROBE MISSION SAMPLE 2

For questions 56-60, determine whether the statement is true or false.

56. When yeast produce alcohol, oxygen is used.
57. Sauerkraut are made by controlled fermentation of cabbage.
58. Bacteria and fungi play a key role in food spoilage.
59. Microbes are used in the production of chocolate.
60. Lowering the temperature of food increases mold growth.

PART 7: Important Microbes

Use the Key of Microbes to identify the Microbe Group
to which each of the important microbes belong
A. Bacteria
B. Fungi
C. Virus
D. Algae (Plant-like protists)
E. Protozoa (Animal-like protists)

61. Baker’s Yeast

62. Diatoms that form diatomaceous earth
63. Oil eating microbes
64. Cheese making Lactobacillus
65. Sporozoa causing Malaria
66. Dinoflagellates that cause red tide
67. Paramecium
68. Zooplankton in the ocean
69. Ruin ¼ to ½ of fruits and vegetables per year
70. Used in sewage treatment plants to breakdown sewage

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PART 8: Microbes and Ecology
Examine the Ocean Food Web and Ocean Energy Pyramid when answering questions 71-80.
71. Which type of organisms would be the primary producers and carry out photosynthesis?

72. Which of the following types of microbes could be primary producers in the ocean? –
Cyanobacteria, protozoa, fungi, algae

73. Which of the following types of microbes could be zooplankton?

Cyanobacteria, protozoa, fungi, algae

74. Which of the following types of microbes could be decomposers for the ocean ecosystem ? –
bacteria, protozoa, fungi, algae

75. What do carnivorous zooplankton eat?

76. What do herbivorous zooplankton eat?

77. Which types of microbes will eat any dead and decaying material that drops to the bottom of the ocean?
– bacteria, protozoa, fungi, algae

78. What percent of the energy available to each trophic level will be still available to the next trophic level?

79. If 8000 energy units were produced by the phytoplankton,

how many units would be available for the Carnivorous zooplankton?

80. If 8000 energy units were produced by the phytoplankton, how many units would be available for the
tuna?

Food Web Energy Pyramid

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PART 9: Microbes and Disease

Key:
A. Fungi
B. Virus
C. Bacteria
D. Protozoan
E. Prion
Use the above key to indicate which type of microbe causes the disease listed in questions 81-90.

81. Typhus

82. Herpes

83. Tetanus

84. Ringworm

85. Anthrax

86. Athlete’s foot

87. Mad Cow Disease

88. Mononucleosis

89. Lyme Disease

90. Malaria

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PART 10 : Dichotomous Key

For questions 91-100, identify the specimen & give the name from the key
91. What is specimen A ?
92. What is specimen B ?
93. What is specimen C ?
94. What is specimen D ?
95. What is specimen E ?
96. What is specimen F ?
97. What is specimen G ?
98. What is specimen H ?
99. What is specimen I ?
100. Where would you find these organisms living?

Protozoa specimens

Dichotomous Key to Selected Protozoa

1. Pseudopods for locomotion. . . . . . . . . . . . . . . . . . . 2
1. Other means for locomotion . . . . . . . . . . . . . . . . . . 3
2. Dome-shaped shell around cell. . . . . . . . . . . . . Difflugia
2. Without shell, many small nuclei . . . . . . . . . . Pelomyxa
3. Cell has flagella . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
3. Cell has cilia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
4. One flagellum . . . . . . . . . . . . . . . . .. . . . . . . . . Peranema
4. Two flagella . . . . . . . . . . . . . . . . . . . . . . . . . . . .Chilomonas
5. Cilia on stalk . . . . . . . . . . . . . . . . . . . . . . . . . . . .Vorticella
5. Cilia not on stalk . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
6. Cilia covering cell . . . . . . . . . . . . . . . . . . . . . . . . . 7
6. Cilia in two bands . . . . . . . . . . . . . . . . . . . . . . . Didinium
7. Cell is cone-shaped . . . . . . . . . . . . . . . . . . . . . . Stentor
7. Cell is elongated . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
8. Cigar-shaped cell with flattened end . . . . . . . . Spirostonum
8. Pear-shaped cell . . . . . . . . . . . . . . . . . . . . . . . Blepharisma

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TEAM NUMBER
SCHOOL
STUDENT NAMES: (PLEASE PRINT) RAW SCORE
1. RANK
2.
BE SURE TO INCLUDE APPROPRIATE UNITS WITH ALL ANSWERS!!!

PART 1: Microscopy PART 4: Cell Types

1. 31.
2. 32.
3. 33.
4. 34.
5. 36.
7. 37.
8. 38.
9. 39.
10. 40.

PART 2: Microscope Types PART 5: Major Groups

11. 41.
12. 42.
13. 43.
14. 44.
15. 45.
16. 46.
17. 47.
18. 48.
19. 49.
20. 50.

PART 3: Relative Size

21.
22. TOTAL PARTS 1-5 _______
23.
24. TOTAL PARTS 6-10 _______
25. TIE-BREAKER
26.
27. RAW SCORE
28.
29.
30.

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PART 6: Microbes & Food PART 9: Microbes & Disease

51. 81.
52. 82.
53. 83.
54. 84.
55. 85.
56. 86.
57. 87.
58. 88.
59. 89.
60. 90.

PART 7: Important Microbes PART 10: Dichotomous Key

61. 91.
62. 92.
63. 93.
64. 94.
65. 95.
66. 96.
67. 97.
68. 98.
69. 99.
70. 100.

PART 8: Microbes & Ecology

71.
72. TOTAL PARTS 6-10
73.
74.
75.
76.
77.
78.
79.
80.

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 MICROBE MISSION SAMPLE 2

TEAM NUMBER
ANSWER KEY SCHOOL
STUDENT NAMES: (PLEASE PRINT) RAW SCORE
1. RANK
2. POINTS

BE SURE TO INCLUDE APPROPRIATE UNITS WITH ALL ANSWERS!!!

PART 1: Microscopy PART 4: Cell Types

1. 400X 31. C
2. 40X to 400X 32. A
3. P 33. B
4. B (Diaphram) 34. B
5. D (Nosepiece) 35. C
6. D (larger & darker) 36. B
7. 7 o’clock 37. A
8. ~ 1.5 mm ~1500 mcm 38. B
9. protozoa 39. A
10. ~ 500 mcm 40. D

PART 2: Microscope Types PART 5: Major Groups

11. D 41. F
12. E 42. E
13. A 43. G
14. B 44. A
15. C 45. D
16. D 46. B
17. C 47. F
18. E 48. D
19. A 49. G
20. B 50. C

PART 3: Relative Size

21. 1000 mcm
22. 1000 nm
23. 1,000,000 nm
24. 800,000 nm
25. 10,000 nm
26. 2,000 nm
27. 1,000 nm
28. 20
29. 200
30. 16,000

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Answer Key
PART 6: Microbes & Food PART 9: Microbes & Disease
51. B 81. C
52. A 82. B
53. B 83. C
54. A 84. A
55. A,B,C 85. C
56. false 86. A
57. true 87. E
58. true 88. B
59. true 89. C
60. false 90. D

PART 7: Important Microbes PART 10: Dichotomous Keys

61. B 91. Pelomyxa
62. D 92. Stentor
63. A 93. Spirostonum
64. A 94. Chilomonas
65. E 95. Didinium
66. D 96. Difflugia
67. E 97. Peranema
68. E 98. Vorticella
69. B 99. Blepharisma
70. A 100. in water (pond)

PART 8: Microbes & Ecology

71. phytoplankton
72. cyanobacteria algae
73. protozoa
74. bacteria fungi
75. herbivorous zooplankton
76. phytoplankton
77. bacteria & fungi
78. 10%
79. 80 energy units
80. 0.8 energy units

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