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    Rappaport Vassiliadis Broth is a selective enrichment medium for isolating Salmonella species from food and environmental samples. It contains soy peptone, potassium phosphates, magnesium chloride, and malachite green to inhibit bacteria other than Salmonella. When used according to European Pharmacopoeia methods after pre-enrichment in Trypticasein Soy Broth, it enriches for Salmonella at 30-35°C in 18-24 hours, which can then be isolated on XLD Agar and identified using biochemical tests. Quality control tests confirm its selectivity for Salmonella typhimurium and inhibition of Staphylococcus aureus.

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    1414 en 1

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    Rappaport Vassiliadis Broth is a selective enrichment medium for isolating Salmonella species from food and environmental samples. It contains soy peptone, potassium phosphates, magnesium chloride, and malachite green to inhibit bacteria other than Salmonella. When used according to European Pharmacopoeia methods after pre-enrichment in Trypticasein Soy Broth, it enriches for Salmonella at 30-35°C in 18-24 hours, which can then be isolated on XLD Agar and identified using biochemical tests. Quality control tests confirm its selectivity for Salmonella typhimurium and inhibition of Staphylococcus aureus.

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    Rappaport Vassiliadis Broth is a selective enrichment medium for isolating Salmonella species from food and environmental samples. It contains soy peptone, potassium phosphates, magnesium chloride, and malachite green to inhibit bacteria other than Salmonella. When used according to European Pharmacopoeia methods after pre-enrichment in Trypticasein Soy Broth, it enriches for Salmonella at 30-35°C in 18-24 hours, which can then be isolated on XLD Agar and identified using biochemical tests. Quality control tests confirm its selectivity for Salmonella typhimurium and inhibition of Staphylococcus aureus.

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    1414 en 1

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    azedaa
    Rappaport Vassiliadis Broth is a selective enrichment medium for isolating Salmonella species from food and environmental samples. It contains soy peptone, potassium phosphates, magnesium chloride, and malachite green to inhibit bacteria other than Salmonella. When used according to European Pharmacopoeia methods after pre-enrichment in Trypticasein Soy Broth, it enriches for Salmonella at 30-35°C in 18-24 hours, which can then be isolated on XLD Agar and identified using biochemical tests. Quality control tests confirm its selectivity for Salmonella typhimurium and inhibition of Staphylococcus aureus.

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    Rappaport Vassiliadis Broth EP/USP Cat.

    1414
    Enrichment medium for Salmonella

    Practical information
    Aplications Categories
    Selective enrichment Salmonella

    Industry: Water / Pharmaceutical/Veterinary / Food / Quality Control

    Regulations: USP / European Pharmacopoeia

    Principles and uses


    Rappaport Vassiliadis Broth is recommended as the selective enrichment medium when isolating Salmonella species from food and environmental
    specimens and is recommended by the European Pharmacopoeia in Paragraph 2.6.13 “Microbiological examination of non-sterile products: test for
    specified microorganisms” for the test of Salmonella in products.

    Soy peptone provides nitrogen, vitamins and amino acids, essential nutrients for growth. Potassium phosphates balance the low pH of the medium,
    combined with the presence of magnesium chloride to raise the osmotic pressure, and malachite green to inhibit other organisms.

    This medium has been found to be superior to other Salmonella selective enrichment media, especially when small inocula and a preenrichment broth
    are used.

    Formula in g/L
    Dipotassium phosphate 0,4 Magnesium chloride anhydrous 13,58
    Malaquite green 0,036 Monopotassium phosphate 0,6
    Sodium chloride 8 Soy peptone 4,5

    Preparation
    Suspend 27,11 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until
    complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 115 ºC for 15 minutes.

    Instructions for use


    For the test for specified microorganisms (Salmonella) according to European Pharmacopoeia:
    - After preenrichment with Trypticasein Soy Broth (TSB) (Cat. 1224), transfer 0,1 ml to 10 ml of Rappaport Vassiliadis Broth.
    - Incubate at 30-35 ºC for 18-24 hours.
    - Subcultivate in plates of XLD Agar (Cat. 1080) and incubate at 30-35ºC for 18-48 hours.
    - The possible presence of Salmonella is indicated by the growth of well developed, red colonies with or without black centers. These results can be
    confirmed with Identification tests.
    - The product complies with the test if colonies of the types described are not present, or if the confirmatory identification tests are negative.

    Quality control
    Solubility Appareance Color of the dehydrated medium Color of the prepared medium Final pH (25ºC)
    w/o rests Fine powder Blue greenish Blue 5,2±0,2

    Microbiological test
    According to European Pharmacopoeia:
    Incubation conditions: (30-35 ºC / 18-24 h).

    Inspired by knowledge Page 1 of 2 - Revision number 1 - Date 25/01/2021 www.condalab.com


    Inoculation conditions: Productivity (<=100CFU) / Inhibitory ( >=100 CFU).

    Microorganisms Specification
    Salmonella typhimurium ATCC 14028 Good growth, turbidity
    Staphylococcus aureus ATCC 6538 Inhibited

    Storage
    Temp. Min.:2 ºC
    Temp. Max.:25 ºC

    Bibliography
    European Pharmacopeia 9.0

    Inspired by knowledge Page 2 of 2 - Revision number 1 - Date 25/01/2021 www.condalab.com

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