C 1 3 2025 Topic Test Ms

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First exams in 2025

IB Style Test – SL/HL Topic C1.3 Photosynthesis


Theme C: Interaction and independence Level of Organisation: Molecules

Name __________________Time allowed: 25 mins SL / 35 mins HL Mark: / 24(SL) 30(HL)


Mark scheme
Multiple choice questions (4 marks)

How is energy from sunlight transformed during the process of photosynthesis?

A. Light is converted to heat energy.


B. Light energy is converted to chemical energy.
C. Light energy is converted to organic energy.
D. Electrons are converted into chemical energy.

1. What is the source of the oxygen produced in photosynthesis

A. Carbon dioxide
B. Glucose.
C. Water
D. Starch

2. Which of the following is the best description of a limiting factor of photosynthesis?


A. A factor which stops a process from happening.
B. A factor which slows a process.
C. A factor which inhibits a process.
D. One of several factors that can limit the rate of a process.

3. If a crop is being grown in a greenhouse, which of the following could be used to increase
growth rate of the crop?

I. Increased atmospheric carbon dioxide concentration.


II. Light bulbs emitting green light.
III. Controlled mineral ion concentration of the soil.
IV. Spraying with herbicides.

A. I only
B. I and III only
C. II and III only
D. I only

© Richard Scarr & David Faure, InThinking www.thinkib.net/biology


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First exams in 2025
IB Style Test – SL/HL Topic C1.3 Photosynthesis
Theme C: Interaction and independence Level of Organisation: Molecules

Structured answer questions (12 marks)


5. The diagram shows the process of chromatography and the result achieved using leaves of a
potato plant.

I. On the diagram above, draw lines to indicate the measurements to be taken to determine
the Rf value of xanthophyll and use these measurements to find the Rf value. (2 marks)

The diagram shows the lines showing the measurements


The calculation: Rf value = b/a.
II. Outline the value of chromatography as a research tool for studying living systems.(2 marks)
Chromatography can separate dissolved solutes.
Chromatography can be used to identify substances by their Rf value.
Chromatography is used in DNA profiles / to separate amino acids / other example.
Biological materials are often in solution with other materials.

© Richard Scarr & David Faure, InThinking www.thinkib.net/biology


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First exams in 2025
IB Style Test – SL/HL Topic C1.3 Photosynthesis
Theme C: Interaction and independence Level of Organisation: Molecules

6. The diagram shows a Photosynthometer which measures the volume of oxygen given off by a
water plant during photosynthesis. The oxygen accumulates in the stem of the plant and rises to the
collection point as a bubble. It can then be drawn into the capillary tube by the syringe. The volume
of gas produced is measured in the capillary tube using the mm scale.

a. List three limiting factors of photosynthesis that this apparatus could investigate (3 marks)
Carbon dioxide (sodium hydrogen carbonate) concentration.
Temperature.
Light intensity.
Light wavelength/colour.

b. For one of the limiting factors that you have listed, outline how you would manipulate the
variables to carry out the investigation. (2 marks)
Repeat the experiment each time with a different value of the test variable. (e.g. the sodium
hydrogen carbonate concentration for carbon dioxide/ the temperature of the water
bath/the distance or intensity of the light source/ use of filters on the light source. (1 mark).
Include a wide enough range of values to show the effect of differences. (1 mark)
Keep the other variables constant. (1 mark)

c. Outline one methodological inaccuracy when using the apparatus. (1 marks)


Not all the oxygen released may be collected by the syringe.
The length of the bubble is not exactly volume.
There may be temperature variation in the water bath (due to the light source).
(Note: only one inaccuracy required for one mark)

d. List two measurements (other than the volume of oxygen) that must be made to calculate
the rate of photosynthesis in a particular set of conditions. (2 marks)
The duration of the experiment, (the length of time).
Mass of water plant/leaf surface area of water plant

© Richard Scarr & David Faure, InThinking www.thinkib.net/biology


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First exams in 2025
IB Style Test – SL/HL Topic C1.3 Photosynthesis
Theme C: Interaction and independence Level of Organisation: Molecules

Extended response questions (8 marks)


7. Outline why the absorption spectrum of chlorophyll pigments is closely aligned to the action
spectrum of photosynthesis. (4 marks)

The action spectrum shows the rate of photosynthesis at different light intensities (of the visible
spectrum).
The absorption spectrum shows the amount of light energy absorbed by chlorophyll at each light
intensity.
Not all wavelengths (of light) are absorbed by chlorophyll.
The energy absorbed by chlorophyll is used to excite electrons.
Excited electrons produce ATP/chemical energy used by photosynthesis.
Geen light has the lowest absorption and lowest rate of photosynthesis/violet light has the
highest absorption and highest rate of photosynthesis.

8 Explain why an understanding of the limiting factors of photosynthesis is important for the
successful production of crops grown in greenhouses. (4 marks)
Greenhouses have controlled environments/the plants can be grown in the most suitable
environment for the fastest growth.
Investigations can be carried out for particular crops to find out the optimal conditions for
photosynthesis.
Optimal photosynthesis rate gives maximum growth of the crop. (2 marks maximum)
The limiting factors that can be controlled/whose effect on photosynthesis can be minimised
include:
Carbon enrichment, the increase in carbon dioxide in the atmosphere of the greenhouse.
The light intensity by lighting the greenhouse.
Optimal temperature of the greenhouse for enzyme function. (2 marks maximum)

© Richard Scarr & David Faure, InThinking www.thinkib.net/biology


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First exams in 2025
IB Style Test – SL/HL Topic C1.3 Photosynthesis
Theme C: Interaction and independence Level of Organisation: Molecules

Higher level extended response questions (6 marks)

9 Photosystems are made of arrays of pigment molecules arranged in membranes.


Discuss the importance of the membranes of the chloroplast in the efficient transformation of
light energy into the energy in ATP. (6 marks)

(Notes: Six of these points is required for six marks)


Chlorophyll is attached to the thylakoid membrane.
The thylakoid membrane links chlorophyll, the electron transport chain and ATP synthase.
Electrons in the chlorophyll molecule are excited by light energy.
The excited electrons are accepted by the electron acceptors positioned in the membrane.
Light energy is used in the photolysis of water to form protons (and electrons)
Photolysis of water occurs in Photosystem II.
Protons formed from photolysis are pumped into the intermembrane space.
The membrane allows an accumulation of protons in the intermembrane space. (Accept; the
creation of a proton gradient, or an electrical potential gradient.)
The proton concentration gradient drives ATP production/ATP is produced by chemiosmosis.
The membrane is impermeable to hydrogen ions (The membranes is only permeable to hydrogen
ions at ATP synthase ‘pores’.)
ATP is produced as the hydrogen ions move through the ATP synthase embedded in the membrane.

© Richard Scarr & David Faure, InThinking www.thinkib.net/biology


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