Riscos Biológicos: Trabalho Prático 2

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Mestrado em Engenharia Biomédica / Mestrado em Biotecnologia 2023/2024

Riscos Biológicos

Trabalho prático 2

 1- Indicate is the following sentences are true or false and correct the false ones
1.1 Biological risk depends only on the type of biological agent, transmission mode and
infectious dose
1.2 Endotoxins are virulence factor present only in gram negative bacteria
1.3 Adhesins enable bacteria invading the epithelium
1.4 Yeasts are capable of invading epithelium due to its capacity to proliferate at 37ºC
1.5 The taxonomy of viruses is based only on the type of nucleic acid
1.6 The permanent hosts (hospedeiro definitivo) of Plasmodium falciparum are humans
1.7 Amibes are unicelular pathogens

 2 Based on the summary of the review article bellow, answer to the following questions:

Lee et al., Front Cell Infect Microbiol. 2017; 7: 55.

2.1 Which are the virulence factors that contribute to the pathogenesis of A. baumannii
mentioned
2.2 Explain why “metal acquisition system” can be considered a virulence factor
2.3 Why this species is particularly problematic in the hospitals.

 3 The following figure shows Candida albicans penetrating human epithelium. Based on what
you see in the Figure name the virulence factors associated with this specie.
Mestrado em Engenharia Biomédica / Mestrado em Biotecnologia 2023/2024

Riscos Biológicos

 4 Select the correct(s) answer(s):


4.1Which of the following statements regarding Cryptosporidium parvum is correct?
A is a pluricellular protozoa
B. is transmited by oral-fecal route
C causes blood diarrhea
D Resist to harsh conditions by the formation of cysts
E all the above are correct
4.2 Give three reasons, by order of importance that may explain the high number of cases of
Malaria in Africa
4.3 Which of the following statements regarding malaria is correct?
A Is caused by the parasite Plasmodium sp
B Is transmited by a mosquito, which is the secondary host
C Is transmited by the oral-faecal route
D. Develops asexual multiplication at the mosquito salivary glands
E All the above are correct
 5 Observe the images and complete the Table
A B C D

Name of the Definite host Intermediate Host Disease(symptoms &


parasite outcomes)
A.
B.
C.
D.

5 Select the correct answer


1. A virus consists of: 3. Why do some viruses seem to go away and then
A.RNA or DNA and a cell membrane return later?
B.RNA or DNA and a protein coat A.host has been infected with a different strain
C.RNA and DNA and a protein coat B.the immune system forgot the virus
D.proteins, cell membrane and RNA C.the virus had entered the lysogenic cycle
D.the virus mutated

2. Retroviruses are different from viruses in that: 4. Why is it difficult to develop vaccines for retroviruses?
A.they have RNA instead of DNA A.their small size evades the immune system
B.they can become dormant B.RNA mutates more frequently than DNA
C.they are symmetrical C.the capsid of retroviruses is resistant
D.all of these D.vaccines can only target blood-borne pathogens
E. None of the above

9. E. coli is a commensal or a pathogenic bacteria? Justify your answer

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