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    Biochemical Engineering Toutorial Questions

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    bereket tekle
    The document contains three questions about evaluating Michaelis-Menten kinetic parameters from experimental data using Lineweaver-Burk, Eadie-Hofstee, and best fit plots. It also contains two assignment questions about calculating substrate concentrations over time in batch and continuous flow reactors using given Michaelis-Menten kinetics.

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    Biochemical Engineering Toutorial Questions

    Uploaded by

    bereket tekle
    80 views3 pages
    The document contains three questions about evaluating Michaelis-Menten kinetic parameters from experimental data using Lineweaver-Burk, Eadie-Hofstee, and best fit plots. It also contains two assignment questions about calculating substrate concentrations over time in batch and continuous flow reactors using given Michaelis-Menten kinetics.

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    Biochemical Engineering Toutorial questions

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    The document contains three questions about evaluating Michaelis-Menten kinetic parameters from experimental data using Lineweaver-Burk, Eadie-Hofstee, and best fit plots. It also contains two assignment questions about calculating substrate concentrations over time in batch and continuous flow reactors using given Michaelis-Menten kinetics.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    80 views3 pages

    Biochemical Engineering Toutorial Questions

    Uploaded by

    bereket tekle
    The document contains three questions about evaluating Michaelis-Menten kinetic parameters from experimental data using Lineweaver-Burk, Eadie-Hofstee, and best fit plots. It also contains two assignment questions about calculating substrate concentrations over time in batch and continuous flow reactors using given Michaelis-Menten kinetics.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Department of Chemical Engineering

    Tutoria and Assignment questions, Biochemical Engineering


    Apr. 1, 2024

    1. An intern chemical engineering student in factory X measured the initial reaction rate of
    hydrolysis alpha bonds of large, alpha-linked polysaccharides (substrate) by Alpha-
    amylase, (α-amylase) and obtained the following data:

    Substrate concentration (mol/L) Initial reaction rate (mol/L min


    0.0032 0.111
    0.0049 0.148
    0.0062 0.143
    0.0080 0.166
    0.0095 0.200

    Evaluate the Michaelis-Menten kinetic parameters by employing


    a) the Langmuir plot,
    b) the Lineweaver-Burk plot,
    c) the Eadie-Hofstee plot

    2. A carbohydrate (S) decomposes in the presence of an enzyme (E). The Michaelis-Menten


    kinetic parameters were found to be as follows:
    KM =200 mol/m3
    rmax =100 mol/m3min
    a) Calculate the change of substrate concentration with time in a batch reactor the initial
    substrate concentration is 300 mol/m3
    b) Chemostat (continuously stirred-tank reactor) runs with various flow rates were
    carried out. If the inlet substrate concentration is 300 mol/m3 and the flow rate is 100
    cm3/min, what is the steady-state substrate concentration of the outlet? The reactor
    volume is 300 cm Assume that the enzyme concentration in the reactor is constant so
    that the same kinetic parameters can be used.
    Department of Chemical Engineering
    Tutoria and Assignment questions, Biochemical Engineering
    Apr. 1, 2024

    3. You carried out an enzymatic reaction in a 5-L CSTR. The inlet substrate concentration
    was 100mmol/L and the flow rate was set 1 L/hr. After a steady-state was reached, the
    outlet substrate concentration was 10 mmol/L.
    a) What is the reaction rate in the reactor?
    b) You measured the steady-state outlet substrate concentration as a function of the inlet
    flow rate and found the following results. Estimate Michaelis kinetic parameters by using
    the best plotting technique for the equal weight of all data points.
    Flow rate (L/hr) Outlet substrate concentration (mol/L)
    0.7 4.0
    0.8 7.0
    1.0 10.0
    1.16 14.0

    Assignment I
    1. Substrate is converted to a product by the catalytic action of an enzyme. Assume that the
    Michaelis-Menten kinetic parameters for this enzyme reaction are:
    Km =0.03 mol/L
    rmax = 13 mol/L min
    a) What should be the size of a steady-state CSTR to convert 95 percent of incoming
    substrate =10 mol/L) with a flow rate of 10L/hr?
    b) What should be the size of the reactor if you employ a plugflow reactor instead of the
    CSTR in part (a)?
    2. A substrate is decomposed in the presence of an enzyme according to the Michaelis-
    Menten equation with the following kinetic parameters:
    KM = 10 g/L
    rmax = 7 g/L min
    If we operate two one-liter CSTRs in series at steady state, what will be the concentration
    of substrate leaving the second reactor? The flow rate is 0.5 L/min. The inlet substrate
    concentration is 50 g/L and the enzyme concentration in the two reactors is maintained at
    Department of Chemical Engineering
    Tutoria and Assignment questions, Biochemical Engineering
    Apr. 1, 2024

    the same value all of the time. Is the two-reactor system more efficient than one reactor
    whose volume is equal to the sum of the two reactors?

    Submission date: Apr 15, 2024

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