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    Biotech Principles and Processesedu

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    Kruti Oza

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    Biotech Principles and Processesedu

    Uploaded by

    Kruti Oza
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    1730588980304_BIOTECH_PRINCIPLES_AND_PROCESSESEDU

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    Biotech Principles and Processesedu

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    Kruti Oza

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    © All Rights Reserved
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    INDIAN LANGUAGE SCHOOL

    GRADE XII SUB-BIOLOGY


    WORKSHEET- BIOTECHNOLOGY PRINCIPLES AND PROCESSES
    Q. 1 Restriction enzymes should not have more than one site of action in the cloning site
    of a vector. Comment.
    Q. 2What does ‘competent’ refer to in competent cells used in transformation
    experiments?
    Q. 3 What is the significance of adding proteases at the time of isolation of genetic
    material (DNA)?
    Q. 4 While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the
    process?
    Q. 5 A plasmid DNA and a linear DNA (both are of the same size) have one site for a
    restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid
    shows one DNA b and, while linear DNA shows two fragments. Explain.
    Q. 6 How does one visualise DNA on an
    agarose gel?
    Q. 7 A plasmid without a selectable marker was
    choosen as vector for cloning a gene. How does
    this affect the experiment?
    Q.8 Identify and explain A, B and C in the PCR
    diagram given .
    Q. 10 Name the regions marked A, B and C.
    Q. 11. Illustrate the
    design of a
    bioreactor.
    Highlight the
    difference between a flask in your laboratory and a bioreactor
    which allows cells to grow in a continuous culture system.

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