DOC/LP/01/28.02.

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LESSON PLAN
SubCode & Name : 1011 IMMUNOTECHNOLOGY Unit: I Branch: Biotechnology Semester : II

LP- BT 1011 LP Rev. No: 00 Date: 27/06/11 Page 1 of 8

Course Objective and Scope: Immunotechnology is a specialized course which deals with biotechnological applications. The immunological mechanisms are introduced in the earlier course BT- 1402 IMMUNOLOGY which deals on immune systems and their components at the cellular and humoral levels. Moreover, at the molecular level, focusing on gene rearrangement of immunoglobulin genes and T-cell receptor genes, and additionally, antigen processing and presentation, cellular responses, innate immunity and tolerance are included in that course. Knowledge on the fundamentals of immunology forms the basis of the course immunotechnology which deals with the practical aspects such as the production and engineering of antibodies, the application of antigens, the design of (recombinant) vaccines, strategies for immune intervention, etc. The Immunotechnology-based techniques will be used in the medicine for therapeutics and diagnostics, industries in the production, quality control and quality assurance, and in R&D Learning outcomes: After this course, the students will be able to: - understand the techniques like immunodiagnostic tests, characterization of lymphocytes, purification of antigens, antibody engineering, etc. - assess health problems with immunological background; - develop approaches for the immune intervention of diseases;

LESSON PLAN
SubCode & Name : BT 1011 IMMUNOTECHNOLOGY Unit: I Branch: Biotechnology Semester : VIII

LP- BT 1011 LP Rev. No: 00 Date: 27/06/11 Page 2 of 8

UNIT I:

ANTIGENS

Syllabus: Types of antigens, their structure, preparation of antigens for raising antibodies, handling of animals, adjuvants and their mode of action. Objectives: 1.To explain the different types of antigen and their structure 2. To describe the preparation of antigens for raising the antibodies and explain the role of adjuvants and their mode of action. 3. To explain the different methods of handling of animals for immunological studies Session No 1. 2. 3. TB/ RF Teaching Aids

Topics to be covered Types of antigens and their structure

Time (min) 50

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Preparation of antigens for raising antibodies; 50 adjuvants and their mode of action Handling of animals 50

LESSON PLAN
SubCode & Name : BT 1011 IMMUNOTECHNOLOGY Unit: II Branch: Biotechnology Semester : VIII

LP- BT 1011 LP Rev. No: 00 Date: 27/06/11 Page 3 of 8

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UNIT II:

ANTIBODIES & IMMUNODIAGNOSIS

Syllabus: Monoclonal and polyclonal antibodies their production and characterization, western blot analysis, immuno electrophoresis, SDS-PAGE, purification and synthesis of antigens, ELISA-principle and applications, radio immuno assay (RIA) principles and applications, non isotopic methods of detection of antigens-enhanced chemilluminescence assay Objectives: 1. To make the student to learn the basis of the production and characterization of monoclonal antibodies 2. To make the student to learn the basis of the production and characterization of polyclonal antibodies. 3. To understand the techniques western blot analysis, immuno electrophoresis, SDS-PAGE ELISA-principle and applications, radio immuno assay and chemilluminescence assay Session No 1 2 3 4 5 6 7 8 Topics to be covered Polyclonal antibodies; their production and characterization Monoclonal antibodies; their production and characterization I Monoclonal antibodies; their production and characterization II SDS-PAGE; Western blot analysis Immuno-electrophoresis Purification and synthesis of antigens ELISA - principle and applications Radio immuno assay (RIA) principles and applications Time 50 50 50 50 50 50 50 50 TB/ RF TB1/ TB2 TB1/ TB2 TB1/ TB2 TB1/ TB2 TB1/ TB2 TB1/ TB2 TB1/ TB2 TB1/ TB2 Teaching Aids LCD/ BB/OHP LCD/ BB/OHP LCD /BB/OHP LCD /BB/OHP LCD /BB/OHP LCD /BB/OHP LCD/ BB/OHP LCD/ BB/OHP

Non-isotopic methods of detection of antigens-enhanced chem. iluminescence LESSON PLAN assay SubCode & Name : BT 1011 IMMUNOTECHNOLOGY Unit: III Branch: Biotechnology

50

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Semester : VIII

UNIT III: ASSESMENT OF CELL MEDIATED IMMUNITY Syllabus: Identification of lymphocytes and their subsets in blood. T cell activation parameters, estimation of cytokines, macrophage activation, macrophage microbicidal assays, in-vitro experimentation-application of the above technology to understand the pathogenesis of infectious diseases. Objectives: To make the students: 1 to understand the methods for identification of lymphocytes and their subsets in blood 2. To understand the mechanism of T cell activation 3. To understand the methods of estimation of cytokines 4. To understand the mechanism of macrophage activation 5. To understand the macrophage micobicidal assays used to understand the pathogenesis of different infectious diseases. Session No 1 2 3 4 5 6 7 8 9 10 11 12 Topics to be covered Identification of lymphocytes and their subsets in blood I Identification of lymphocytes and their subsets in blood II Identification of lymphocytes and their subsets in blood III T cell activation parameters I T cell activation parameters II Estimation of cytokines I Estimation of cytokines II Macrophage activation- I Macrophage microbicidal assays I Macrophage microbicidal assays II Macrophage microbicidal assays III Macrophage microbicidal assays Time 50 50 50 50 50 50 50 50 50 50 50 50 TB/ RF TB1/T B2 TB1/T B2 TB1/T B2 TB1/T B2 TB1/T B2 TB1/T B2 TB1/ TB2 TB1/T B2 TB1/ TB2 TB1/ TB2 TB1/T B2 TB1/ Teaching Aids LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ LCD/ LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP

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LESSON PLAN
SubCode & Name : Unit: IV BT 1011 IMMUNOTECHNOLOGY Branch: Biotechnology Semester : VIII

LP- BT 1011 LP Rev. No: 00 Date: 27/06/11 Page 5 of 8

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UNIT IV:

IMMUNOPATHOLOGY

Syllabus: Preparation and storage of tissues, identification of various cell types and antigens in tissues, isolation and characterization of cell types from inflammatory sites and infected tissues, functional studies on isolated cells, immuno cytochemistry immuno fluorescence, immuno-enzymatic and immuno-ferrtin techniques, immuno-electron microscopy Objectives: To make the students to: 1. Understand the preparation and storage of tissues for immunological identification 2. understand the methods for identification of various cell types and antigens in tissues 3. Understand the isolation and characterization of cell types from inflammatory sites and infected tissues 4. to understand the methods for functional studies on isolated cells 5. to learn the techniques of immnocytochemistry Session No 1 2 3 4 5 6 Topics to be covered Preparation and storage of tissues Identification of various cell types and antigens in tissues Isolation and characterization of cell types from inflammatory sites and infected tissues - functional studies on isolated cells Immuno cytochemistry immuno fluoresecence Immuno enzymatic and immuno ferrtin techniques Immuno electron microscopy Time 50 50 50 50 50 50 TB/ RF TB1/ TB2 TB1/ TB2 TB1/ TB2 TB1/ TB2 TB1/ TB2 TB1/ TB2 Teaching Aids LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP

LESSON PLAN
SubCode & Name : BT 1011 IMMUNOTECHNOLOGY Unit: V UNIT V: Branch: Biotechnology Semester : VIII

LP- BT 1011 LP Rev. No: 00 Date: 27/06/11 Page: 6 of 8

MOLECULAR IMMUNOLOGY

Syllabus: Preparation of vaccines, application of recombinant DNA technology for the study of the immune system, production of antidiotypic antibodies, catalytic antibodies, application of PCR technology to produce antibodies and other immunological reagents, immuno therapy with genetically engineered antibodies. Objectives: Make the students 1. to understand the methods of preparation of vaccines 2. Understand the basis and applications of rDNA technology for study of immune system 3. To learn basis and the methods for production of antidiotypic antibodies and catalytic antibodies 4. to understand the the applications of PCR technology 5. to learn about immunotherapy Session No 1 2 3 4 5 6 7 8 9 Teaching Aids LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP

Topics to be covered Preparation of vaccines - I Preparation of vaccines - II Preparation of vaccines - III Application of recombinant DNA technology for the study of the immune system I Application of recombinant DNA technology for the study of the immune system II Application of recombinant DNA technology for the study of the immune system III Production of anti-idiotypic antibodies, catalytic antibodies Application of PCR technology to produce antibodies and other immunological reagents Immunotherapy with genetically engineered antibodies.

Time 50 50 50 50 50 50 50 50 50

TB/ RF RF1 RF1 RF1 RF1 RF1 RF1 RF1 RF1 RF1

UNIT VI:

CURRENT TOPICS IN IMMUNOLOGY

Syllabus: Trends in Immunology of infectious diseases and tumors. Objectives: To make the students 1. to understand the recent trends in immunology of infectious diseases 2. to understand the recent trends in immunology of cancer biology Session No 1 2 3 4 5 6 Teaching Aids LCD/ BB/OHP LCD/ BB/OHP LCD/ LCD/ BB/OHP LCD/ BB/OHP LCD/ BB/OHP

Topics to be covered Trends in Immunology of Infectious diseases -I Trends in Immunology of Infectious diseases- II Trends in Immunology of Infectious diseases- III Trends in Immunology of Infectious diseases- IV Trends in Immunology of Tumors I Trends in Immunology of Tumors II

Time 50 50 50 50 50 50

TB/ RF RF1 RF1 RF1 RF1 RF1 RF1

Course Delivery Plan Week Units 1 I 2 3 II 4 5 6 III 7 8 9 IV 10 11 12 V 13 14 VI 15

Text Books (TB): 1. 2. 1990. References (RF): 1. Austin J.M. and Wood K.J., Principles of cellular and molecular immunology, Oxford university press, Oxford, 1993. Talwar G.P., and Gupta S.K., A hand book of practical and clinical immunology, Vol. 1 & 2, CBS Publications, 1992. Weir D.M., Practical Immunology, Blackwell Scientific Publications, Oxford,

Prepared by Signature Name Designation Date Prof. M. Sivanandham Professor June 28, 2011

Approved by

Prof. M. Sivanandham HOD of Biotechnology June 28, 2011