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    Narrative Report: Basic Micros

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    Mon Ronquillo
    The document summarizes a student laboratory activity where they examined human blood samples under an electron microscope. The students observed blood alone and blood mixed with three saline solutions of different concentrations. They found that the pure blood had moving cells, while the saline mixtures compacted the cells and changed the blood color. The activity helped students learn basic microscopy skills and understand how salt concentration affects blood composition.

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    Narrative Report: Basic Micros

    Uploaded by

    Mon Ronquillo
    722 views3 pages
    The document summarizes a student laboratory activity where they examined human blood samples under an electron microscope. The students observed blood alone and blood mixed with three saline solutions of different concentrations. They found that the pure blood had moving cells, while the saline mixtures compacted the cells and changed the blood color. The activity helped students learn basic microscopy skills and understand how salt concentration affects blood composition.

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    Narrative-report-Basic-Microscopy

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    The document summarizes a student laboratory activity where they examined human blood samples under an electron microscope. The students observed blood alone and blood mixed with three saline solutions of different concentrations. They found that the pure blood had moving cells, while the saline mixtures compacted the cells and changed the blood color. The activity helped students learn basic microscopy skills and understand how salt concentration affects blood composition.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    722 views3 pages

    Narrative Report: Basic Micros

    Uploaded by

    Mon Ronquillo
    The document summarizes a student laboratory activity where they examined human blood samples under an electron microscope. The students observed blood alone and blood mixed with three saline solutions of different concentrations. They found that the pure blood had moving cells, while the saline mixtures compacted the cells and changed the blood color. The activity helped students learn basic microscopy skills and understand how salt concentration affects blood composition.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 3

    Ronquillo, Mon Carlo L.

    G210 – Res03

    Narrative Report: Basic Microscopy

    In this activity, the students tackle about basic microscopy. In basic microscopy, the main

    apparatus that is used is Electron microscope, which is used to enhance or enlarge images of small

    objects that cannot be examined by the naked eyes. Specifically, in this activity, the substance that

    has examined is the blood of humans and for comparison, three saline solutions with different

    concentrations are also used. The three-saline solution are added with the blood to be examined

    and determine what would be the effect of its different in concentration. The main purpose of this

    activity is to give a basic knowledge about microscopy and gives the student a more in-depth

    understanding about the composition of blood and factors that can affect it.

    Week 10 of the immersion of the student, this activity was performed on February 26, 2018

    at N401 with the scheduled time- 3:00 to 4:30 pm. First, the students wore their gloves, lab gown,

    face mask and goggles to avoid any tendency of error to happen and to maintain the safety of the

    students performing the experiment. Afterwards, the materials are prepared and laid out properly

    in the working area. The materials are 10 pieces of Glass slides, 1 pack of cover slips and blood

    lancet, cotton balls, alcohol, Kim wipes, and marking pen. Before the students start the actual

    experiment, the instructor showed the apparatus that will be using in this activity which is the

    electron microscope. The instructor said that there are only 3 microscopes available at that time to

    be used. The instructor also added that the apparatus is set in the appropriate lens (10x) so the

    students should not move anything on it.


    Moving on to the experiment, the students prepared 4 clean microscope that are marked by

    the letters W, X, Y, Z that will stand as W for the pure blood, and X for the blood added by first

    saline solution, Y for the blood added by the second saline solution and Z for blood added by the

    third saline solution. The students picked one of their groupmates, Mr. Tuason to be the source of

    the blood to be examined. Before the students pricked Mr. Tuason. the students used cotton balls

    poured with alcohol to disinfect the area of the finger to be pricked. Ms. De Guzman, the one who

    are assigned to execute it, gently pricked the index finger of Mr. Tuason using the blood lancet

    and carefully put the blood coming out of the finger to the glass slide W. Now, one of the students

    put the cover slips and put it aside. The blood that is still coming out from Mr. Tuason finger is

    added to the glass slide X and after the blood is in the glass slide, the students put one drop of the

    first solution. For the blood that has been used in glass slide Y and Z, the blood comes from one

    of the students’ groupmates- Mr. Ronquillo. Same procedure is done to both glass slides, but they

    differ on the added solution. For the glass slide Y, the second saline solution and for the glass slide

    Z, the last solution.

    The students then now go to the area where the microscope is located. However, when the

    students said to the instructor that the blood used are different in glass slides WX and YZ, the

    instructor stresses that the students should only use one source of blood to have accurate

    comparison of the composition of the blood. The students repeat the procedure on glass slide Y

    and Z but in this time the blood comes from Mr. Tuason. The students then go to the microscope

    area. However, there are group that is still working on it and the students waited for their turn. But,

    since the group that is using the microscope take a lot of time working on it, the blood on the glass

    slides becomes dry. Because of this, the students repeat the experiment but this time the one that

    is been pricked is Mr. Ronquillo because Mr. Tuason hands is getting numb since it is been pricked
    a couple of times. Same procedures are done to Mr. Ronquillo starting glass slide W up to glass

    slide Z.

    The students then observed the formation of cells inside the substance. For the glass slide

    W which is pure blood, the students observed that there are certain parts of the blood sample which

    cells are moving. It is like a river of cells. For the glass slide X which blood added by the first

    solution, the cells are more compacted compared to the results on the glass slide W. Also, there

    are no movement of cells observed in glass slide X. In glass slide Y, which is blood added by the

    second solution, the color of the substance becomes redder compared to the glass slide X and W.

    There also no sign of movement of cells in this glass slide. Lastly, the students observed in glass

    slide Z which is blood added by the last solution. The particles inside the blood sample is the

    smallest among them all. The color is the same with W and X but it is more compacted compared

    to glass slide X that there is no space available for any particle to be formed.

    After observing the blood sample, the glass slides are cleaned in the sink and then throwed

    in the designated waste container located beside the sink. The working area is also cleaned using

    rags and alcohol for the next students to perform in the laboratory. This is the first time the student

    used microscope and observed the composition of blood and it is engaging to see clearly the

    formation of cells inside the substance. It also gives the student a better understanding on the

    effects of different concentration of Sodium Chloride on blood. This activity gives the student an

    on-hand experience and knowledge about how microscope work which surely will be helpful to

    the students in their upcoming college program especially to those in chemical and medicine

    related courses.

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