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    LB-Broth (Miller) : Mode of Action Experimental Procedure

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    Gustavo Cano Gallegos
    LB-Broth is a growth medium formulated by Miller in 1972 that supports the growth of E. coli. It contains yeast extract and peptone from casein which provide nitrogen, carbon, sulfur, minerals, and vitamins for growth. To prepare, suspend 25g of LB-Broth powder in 1 liter of water, autoclave for 15 minutes at 121°C, and incubate E. coli for 24 hours at 35-37°C. Quality control tests show good growth of two E. coli strains.

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    LB-Broth (Miller) : Mode of Action Experimental Procedure

    Uploaded by

    Gustavo Cano Gallegos
    38 views1 page
    LB-Broth is a growth medium formulated by Miller in 1972 that supports the growth of E. coli. It contains yeast extract and peptone from casein which provide nitrogen, carbon, sulfur, minerals, and vitamins for growth. To prepare, suspend 25g of LB-Broth powder in 1 liter of water, autoclave for 15 minutes at 121°C, and incubate E. coli for 24 hours at 35-37°C. Quality control tests show good growth of two E. coli strains.

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    318-lb-broth-miller-110285

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    LB-Broth is a growth medium formulated by Miller in 1972 that supports the growth of E. coli. It contains yeast extract and peptone from casein which provide nitrogen, carbon, sulfur, minerals, and vitamins for growth. To prepare, suspend 25g of LB-Broth powder in 1 liter of water, autoclave for 15 minutes at 121°C, and incubate E. coli for 24 hours at 35-37°C. Quality control tests show good growth of two E. coli strains.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    38 views1 page

    LB-Broth (Miller) : Mode of Action Experimental Procedure

    Uploaded by

    Gustavo Cano Gallegos
    LB-Broth is a growth medium formulated by Miller in 1972 that supports the growth of E. coli. It contains yeast extract and peptone from casein which provide nitrogen, carbon, sulfur, minerals, and vitamins for growth. To prepare, suspend 25g of LB-Broth powder in 1 liter of water, autoclave for 15 minutes at 121°C, and incubate E. coli for 24 hours at 35-37°C. Quality control tests show good growth of two E. coli strains.

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    © All Rights Reserved
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    LB-Broth (Miller)

    For the cultivation of E. coli in fermentation and molecular genetic studies.


    LB-Broth (Miller)

    Mode of Action Experimental Procedure


    LB-Broth is based on the formulation of MILLER (1972) According to appropriate use or purpose.
    supporting growth of E. coli. Incubation: 24 h at 35-37 °C aerobically.
    Casein peptone and yeast extract supply essential growth factors,
    such as nitrogen, carbon, sulfur, minerals and vitamins. Literature
    MILLER J.H.: Experiments in Molecular Genetics, Cold Spring Harbor
    Typical Composition (g/litre) Laboratory (1972).
    LB-Broth: Ordering Information
    Yeast extract 5.0; peptone from casein 10.0; sodium chloride
    10.0. Product Merck Cat. No. Pack size
    LB-Broth (Miller) 1.10285.0500 500 g
    Preparation
    Suspend 25 g LB-Broth in 1 litre of demin. water and autoclave LB-Broth (Miller) 1.10285.5000 5 kg
    for 15 min at 121 °C.
    pH: 7.0 ± 0.2 at 25 °C.
    The prepared broth is clear and yellowish-brown.

    Quality control
    Test strains Growth
    Escherichia coli ATCC 25922 good
    Escherichia coli ATCC 11775 good

    318 Merck Microbiology Manual 12th Edition

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