Lab 40 Cell Membranes 2006

Name _____________________________ Period _________
AP Biology Date ______________________
LAB ____. CELL MEMBRANES

The cell membrane regulates what enters and leaves the cell and also aids in the protection
and support of the cell. In a way, the cell membrane is similar to the walls that surround your
house. As these walls help to protect your house from what is outside so the cell membrane
seals off the cell from its outside environment. But if you lived in the house, you would still want
to receive messages, fuel, and power from the outside environment. So utilities lines like
telephone, gas, and electric would have to be able to pass through the walls of your house. You
would also like to bring in food and take out trash. Thus doors would be needed. The needs of
the cell are similar. It must communicate with other cells, take in food and water, and eliminate
waste. All of these processes take place through the cell membrane.
The cell membrane is composed of several kinds of molecules. The most important of these are
lipids. A double layer — a bilayer — of lipid molecules forms the basic unit from which cell
membranes are constructed. The lipids in the cell membrane are a special type of lipid called
phospholipid. They have a phosphate head and a lipid tail region. The head is hydrophilic
(water loving) and the tail is hydrophobic (water fearing). This is one of the reasons why some
molecules easily pass through the membrane (like O2, CO2, and steroids) and others with
difficulty (carbohydrates, proteins, nucleic acids).
Proteins and carbohydrates are also associated with the cell membrane. Some proteins stick
to the surface of the lipid bilayer — peripheral proteins— whereas others span the membrane
from one side to the other — transmembrane proteins — and are free to move around within
the membrane. This is dependent on their molecular structure. Some of the free-moving
proteins serve as transport proteins — acting as channels or tunnels through which molecules
may pass. Others act like small pumps, actively pushing molecules from one side of the
membrane to the other, much like a revolving door. The carbohydrates are attached to either
proteins or lipids. These act as recognition sites — identifying tags —and allow cells to
recognize other cells from the same organism. These carbohydrates are the reason why an
organ such as a kidney can be rejected after a kidney transplant. It is also the reason why we
can only receive blood transfusions from certain blood types and not from others.
As a result of its structure, our concept of the cell membrane is often described as the Fluid
Mosaic Model. The word fluid refers to the idea that the membrane is actually moving. Most of
the lipids and some of the proteins drift at a rate of approximately 2µm/second. The mosaic
refers to the idea that
the membrane is like
a collage of proteins
and other molecules
embedded in the fluid
matrix (much like
different color tiles
embedded in grout in
a ceramic mural or
floor)
1 of 4
Name _____________________________ AP Biology
CONSTRUCTING A MODEL OF THE CELL MEMBRANE
1. Construct a model of the cell membrane using diagrams you have been provided in your
text, during lecture, and in this lab.
2. You may use construction material provided (assorted food & non-food materials: assorted
pasta, assorted cereals, glue, pipe cleaners, yarn, and more) or supply additional material
on your own.
3. Make sure your model includes the following components:

a. Phospholipid bilayer (heads & tails!)
b. Transport proteins
c. Peripheral proteins
d. Protein pumps
e. Carbohydrates
f. Receptor proteins
g. Cholesterol
4. Make a key to show what each item represents
5. Glue your cell membrane to the paper or cardboard provided.
6. Answer the Summary Questions.
2 of 4
Name _____________________________ AP Biology
SUMMARY QUESTIONS
1. What are the functions of the cell membrane?
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
2. The cell membrane is often described as a bilayer. Explain this term. What two layers make
up the cell membrane?
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
3. Where are proteins found in the cell membrane?
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
4. Explain why the cell membrane is described as a Fluid Mosaic Model.
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
5. Give an example of a molecule that is unable to pass through the cell membrane.
Explain why.
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
3 of 4
Name _____________________________ AP Biology
6. Explain the function of a transport protein.
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
7. Explain the commonalities and difference between active transport and facilitated diffusion
and give an example of each.
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
8. Some of the proteins on the surface of the cell are known as receptor proteins because they
receive messages from outside the cell. Draw a diagram to show a receptor protein and the
signal molecule it receives. (Remember the importance of shape in biology.)
9. What is one possible message that one cell might send to another cell?
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
10. What is the function of cholesterol in the cell membrane?
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
4 of 4

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Name _____________________ Period _

AP Biology Date ______________________

LAB ____. CELL MEMBRANES

CONSTRUCTING A MODEL OF THE CELL MEMBRANE

3. Make sure your model includes the following components:

4. Make a key to show what each item represents

5. Glue your cell membrane to the paper or cardboard provided.

6. Answer the Summary Questions.

1. What are the functions of the cell membrane?

3. Where are proteins found in the cell membrane?

4. Explain why the cell membrane is described as a Fluid Mosaic Model.

6. Explain the function of a transport protein.

10. What is the function of cholesterol in the cell membrane?

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