Lab Sop

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SOUTHERN BETHANY CHRISTIAN

SCHOOL OF MINGLANILLA, INC.


STANDARD OPERATING PROCEDURE (SOP)
Biology Laboratory - Standard Operating Procedure

The following SOPs are to be strictly followed for the safety of students and teachers of
Biology class during the conduct of laboratory activities and lecture classes:
1. The laboratory room will be disinfected daily with 0.5-1% Sodium hypochlorite solution
or sanitizers by the Lab Assistant before and after the experiments.
2. Practical requirements are to be kept ready one day in advance, or well before the start
of the experiment.
3. Letter of Consent from Parents/ Guardians must be submitted to the Dept./H.O.D
before the commencement of the laboratory practical.
4. Thermal scanning and Hand sanitization are to be done before entering the Biology
Laboratory room.
5. Kindly DO NOT come to the Laboratory room if:
a) any member of your family is tested COVID positive.
b) you are suffering from cold and cough, body-ache, throat irritation and congestion.
c) any member of your family visited a COVID positive patient or person.
6. In order to avoid crowding, a maximum of 12-15 students will be allowed in a practical
hall at a time.
7. Please wear a standard safety mask that offers maximum protection.
8. Bring your own personal practical apparatuses. Exchange/ Sharing of any items will not
be allowed.
9. Always maintain minimum distance at the time of instruction, interaction in the
Practical Hall.
10. While performing experiments, you must wait patiently at the time of collection
of chemicals, or any such items.
11. Do not rush while handling acids or such hazardous chemicals.
12. All waste or unused items must be disposed of with care. Use the waste bin provided in
the Hall.
13. Avoid intermingling in the hall.
14. Please handle all Lab equipment/apparatuses with care.
15. Always wash your hands with soap after touching common equipment, apparatuses,
etc.
16. Taking food and meal will not be allowed in the Laboratory room.
SOUTHERN BETHANY
STANDARD OPERATING CHRISTIAN
PROCEDURE (SOP)

SCHOOL OF MINGLANILLA, INC.


STANDARD OPERATING PROCEDURE (SOP)

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Chemistry Laboratory - Standard Operating Procedure

Our prime motto is: “SAFETY FIRST AND FOREMOST”

Chemistry laboratories are potentially dangerous workplace, where staff and students are
involved with handling of some potentially toxic, flammable and explosive compounds,
abnormal temperature and pressure conditions and analytical equipment. Therefore, there are
significant risks of error and mishaps. However, with proper precautions and guidance, even
toxic chemicals can be handled to avoid accidents. With proper awareness of safe working
practices by associated members working in chemistry laboratories, we can ensure a “Safe and
healthy” working environment for all the members associated with the Discipline of Chemistry.
Here, we provide some general guidelines to encourage safe working practices in our
laboratories.

GENERAL LABORATORY SAFETY PROCEDURES:


 In Chemistry laboratory, a student is required to handle chemicals that are carcinogenic,
poisonous, flammable, and explosive.
 Some of the chemicals and equipment may cause severe burns, cuts, or bruises if not
handled properly / carefully.
 Most of the accidents that occur in the chemistry laboratory are a result of carelessness,
impatience, improper or unauthorized handling / experimentation, and negligence of safety
rules and proper operating procedures.
In order to minimize the chance of accidents in the laboratory certain rules and regulations must
be obeyed at all times while one is working or observing in a chemical laboratory. Therefore, it is
not advisable for anyone to work in the laboratory without proper knowledge of the dangers
involved. Due to the inherent dangers present in a chemical laboratory exercise, it should be
understood that the following rules must be obeyed at all times, to minimize chances of any
accident. Students are expected to exercise proper judgment and be extremely cautious at all
times while working in the laboratory.

Students are advised to Learn and follow the following laboratory safety rules and regulations:

1. Be sure that you understand every instruction before proceeding towards Laboratory.
2. DO NOT perform unauthorized experiments or work in the laboratory alone.
3. PPE: Personal Protective Equipment (EYE PROTECTION and CLOTHING): Appropriate
clothing must be worn at all times while in the laboratory. Approved laboratory coat or
apron must be worn. Your legs must be completely covered below the knee by your choice
of clothing. Approved eye protection may be worn in the laboratory. Eye protection must be

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splash proof chemical goggles and be approved by your teacher. Always wear eye protection
in the lab, even if you are not doing any experiment. Hazards can come from other student’s
experiments, not just your own. If you do get any chemical in your eyes, rinse your eyes
immediately with sufficient water.
4. Long hair and loose clothing must be confined while in a laboratory.
5. Closed shoes with socks must be worn at ALL times. Open-toed shoes, backless shoes,
sling backs, clogs, and sandals are not permitted.
6. Know the location and proper use of fire extinguishers, fire blankets, safety showers, eye
wash devices and first aid kits.
7. Before handling any chemicals, read the label on the reagent bottles carefully.
8. Eating, smoking and drinking are not allowed in a chemistry laboratory.
9. Use the fume hoods or perform experiment under exhaust fan when toxic or irritating
vapors are involved.
10. Never force glass tubing through cork or rubber stoppers without proper lubrication.
11. Never direct the open end of test tube / ignition tube toward yourself or anyone else.
12. Never pour water into concentrated acid.
13. Learn the proper procedure for igniting and operating a laboratory burner. Always
extinguish the flame when the burner is not being used. Make sure that all flammable
reagents are well removed before lighting the burner.
14. Chemical wastes must be disposed of properly at all times.
15. Never place chemicals directly on the balance pan. Always use a proper weighing
container when using a balance to weigh a chemical. Never pour chemicals directly over
the balance.
16. Never return unused chemicals to their original container (unless directed to do so by the
instructor).
17. Securely replace lids, caps, and stoppers after removing reagents from containers.
18. Always wipe spatulas clean before and after inserting into reagent bottles.
19. Report any accident and/or injury, however minor, to your instructor immediately.
20. Never place anything that is not directly required for the experiment on laboratory desks;
other items may interfere with the experiment.
21. All personal belongings should be placed in the shelves as you enter the laboratory.
22. Clean up any spill immediately with the help of Laboratory Assistant.
23. Before leaving the laboratory, make sure your work area is clean and dry. Ensure that all
gas, water, vacuum, and air valves are completely turned off.
24. Thoroughly wash your hands before leaving the laboratory.

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25. Your teacher is available for any assistance you may need. Never hesitate to ask
questions especially if there is any question concerning proper operating procedure.
26. In case of any mishap, please contact the following persons
i) Laboratory Assistant / Teacher concerned.
ii) Principal

Principal

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SOUTHERN BETHANY CHRISTIAN
SCHOOL OF MINGLANILLA, INC.
STANDARD OPERATING PROCEDURE (SOP)

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Physics Laboratory - Standard Operating Procedure

The following are the Standard Operating Procedure (SOP) for students, faculty and staff.
General Physics Laboratory Safety Practices

There are many types of experiments carried out in the Physics Laboratory. We will first list the
general SOP applicable at all times.

1. Students must avoid bringing bags into the working area of the laboratory. This is to
prevent accidental damage to the instruments or the bags and to avoid cluttering of the
working table that might disturb the experiment.
2. No instruments or any material is to be taken from the laboratory without the express
permission of the teachers or laboratory assistant.
3. Every object taken from the laboratory must be duly entered in the register.
4. At the end of the experiment, the student must submit the equipment to the laboratory
assistant, even if the experiment is incomplete.
5. Any damage to any equipment must promptly be informed to the teachers or the
laboratory assistant.
6. Prior permission from the teachers must be obtained for performing experiments at
undomesticated.
7. Any injury to any student caused by an equipment must be brought to the notice of the
teachers or laboratory assistant immediately.
8. During emergencies like fire and earthquake, the students, teachers and the laboratory
assistant will follow safety guidelines and exit in an orderly fashion; equipment and bags
can be left behind as health and safety are bigger concerns.

Safety when handling experiments on heat:


1. Currently, either a heater or hot plate is used for doing experiments on heat. Students
should first consult the teachers or the laboratory assistant before starting on such
experiments.
2. The student should be careful at all times during such experiments.
3. Any experiment that involves heat, should never be left unattended. At least one student
from the group should be attending the experiment. In case of any untoward incident like
melting wires, boiling out of the liquid, the student should inform the teachers or the
laboratory assistant immediately and should not try to solve the problem himself/herself.
4. Tongs should be used for handling hot objects.
5. Easily flammable clothes should be avoided when doing heat experiments by both
students and faculty.

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Safety when doing electricity experiments
1. Although we do not have any high voltage experiments, but the students should be
careful when doing electricity experiments.
2. He/she should not touch any wires if his/her hands are wet, even for low voltage
equipment.
3. After many any circuit connection and before switching on the instrument, the student
must show the connections to the teachers or the laboratory assistant. This is to avoid
damage to the instrument due to wrong connections.
4. For instruments requiring battery eliminator, the students should use the lowest voltage
possible and should not exceed 4 Volts generally. Use of higher voltage should be done
with the consent of the teachers or the laboratory assistant.

Safety when doing optical experiments


1. Although we do not have any lasers or high-powered light sources for our experiments but
some precautions should be taken.
2. The students should not strain his/her eyes for extended periods of time when doing
experiments, particularly in the dark room. They should take break of about a minute after
every 10 minutes of work.
3. The sodium bulbs are fragile and hence should be handled with extreme care. The student
should notify the teachers or the laboratory assistant about any problem in the instrument
and should not try to solve the problem himself/herself.
4. The pins used in the optical bench are sharp and should be placed with the pointed part
inside the groove when not in use.

Additional guidelines to be followed in view of the COVID-19 pandemic.


1. Students, the teachers or the laboratory assistant must sanitize his/her hands when
entering the laboratory.
2. Students, the teachers or the laboratory assistant must sanitize his/her hands when
exiting the laboratory after finishing all experiments.
3. Mask should be worn at all times.
4. Social distancing should be maintained at all times.
5. Students, should avoid mixing with people outside of their practical group during
experiments.
6. All library work should be done before starting the experiment.
7. The teachers and the laboratory assistant are advised to wash hands regularly.
8. Every equipment should be sanitized before handing it over to the next group.

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9. Avoid throwing food stuffs in the laboratory dustbin.
10. The rooms should be as much ventilated (by keeping the windows open) as possible
without disturbing the experiments.
11. Smaller groups to be made and experiments should be performed batch-wise.

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