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Stoichiometry and Kinetics of Biochemical Operations

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Sam Denielle Tugaoen

This document discusses stoichiometry and kinetics of biochemical operations. It describes microbial growth cycles, including lag phase, exponential phase, deceleration, stationary phase, and death phase. The exponential phase involves each cell dividing into two cells repeatedly, leading to exponential growth. Eventually, essential nutrients are depleted or waste accumulates, ending exponential growth and entering the stationary phase.

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Stoichiometry and Kinetics of Biochemical Operations

Uploaded by

Sam Denielle Tugaoen
30 views13 pages
This document discusses stoichiometry and kinetics of biochemical operations. It describes microbial growth cycles, including lag phase, exponential phase, deceleration, stationary phase, and death phase. The exponential phase involves each cell dividing into two cells repeatedly, leading to exponential growth. Eventually, essential nutrients are depleted or waste accumulates, ending exponential growth and entering the stationary phase.

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This document discusses stoichiometry and kinetics of biochemical operations. It describes microbial growth cycles, including lag phase, exponential phase, deceleration, stationary phase, and death phase. The exponential phase involves each cell dividing into two cells repeatedly, leading to exponential growth. Eventually, essential nutrients are depleted or waste accumulates, ending exponential growth and entering the stationary phase.

Copyright:

© All Rights Reserved
Download as PDF, TXT or read online from Scribd
Download as pdf or txt
30 views13 pages

Stoichiometry and Kinetics of Biochemical Operations

Uploaded by

Sam Denielle Tugaoen
This document discusses stoichiometry and kinetics of biochemical operations. It describes microbial growth cycles, including lag phase, exponential phase, deceleration, stationary phase, and death phase. The exponential phase involves each cell dividing into two cells repeatedly, leading to exponential growth. Eventually, essential nutrients are depleted or waste accumulates, ending exponential growth and entering the stationary phase.

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STOICHIOMETRY

 AND  
KINETICS  OF  BIOCHEMICAL  
OPERATIONS
STOICHIOMETRY  AND  GENERALIZED  
REACTION  RATE
• STOICHIOMETRY  – concerned  with    relationship  between  quantities  
of  reactants  and  products  in  reaction
• Cultivation-­‐ the  growth  of  microorganisms  population  in  an  artificial  
environment
STOICHIOMETRY  AND  GENERALIZED  
REACTION  RATE
• Pure  culture-­‐a  culture  containing  only  a  single  kind  of  microorganism.
Metabolism  and  growth

• Growth  is  defined  as  an  increase  in  the  number  of  cells.
• binary  fission    -­‐that  two  cells  have  arisen  from  one

generation  time
Mathematics    Of  Exponential  Growth

• The  increase  in  cell  number  in  an  exponentially  growing  bacterial  
culture  approaches  a  geometric  progression  of  the  number  2.
• N  =  N02n
The  Microbial  Growth  Cycle

• Lag  Phase-­‐ beginning  


• Acceleration
• Exponential  Phase-­‐ growth  each  cell  divides  to  form  two  cells,  each  
of  which  also  divides  to  form  two  more  cells,  and  so  on,  for  a  brief  or  
extended  period,  depending  on  the  available  resources  and  other  
factors.
• Decline
• Stationary  Phase-­‐ an  essential  nutrient  of  the  culture  medium  is  used  
up,  or  (2)  a  waste  product  of  the  organism  accumulates  in  the  
medium  and  inhibits  growth.  Either  way,  exponential  growth  ceases
• Death  Phase  -­‐ the  cells  may  remain  alive  and  continue  to  metabolize,  
but  they  will  eventually  die.
GROWTH  CYCLE
GROWTH  CYCLE

• Cell  growth  is  the  primary  response  of  viable  cells  to  substrates  and  
nutrients.  Substrates/nutrients  +  cells  →  products  +  more  cells

• Product  formation  is  a  secondary  response.


LAG  PHASE
Exponential  Phase
DECELERATION
STATIONARY  PHASE
DEATH  PHASE

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